Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,778,364 C→T 5.2% intergenic (+17/+26) ydiO → / ← ydiP putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,3640CT 5.2% 300.0 / 2.6 93intergenic (+17/+26)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP
Reads supporting (aligned to +/- strand):  ref base C (50/38);  new base T (2/3);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGG  >  NC_000913/1778215‑1778511
                                                                                                                                                     |                                                                                                                                                   
gggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGc                                                                                                                                                     >  2:114758/1‑150 (MQ=255)
  gCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGctt                                                                                                                                                   >  2:1100828/1‑150 (MQ=255)
     ataCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttctt                                                                                                                                                >  2:1250774/1‑150 (MQ=255)
        ccGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                                                                             <  1:1355751/150‑1 (MQ=255)
        ccGATGAGCCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTAGTGAAAGTATCGGAGGCGGTACAGACGAAATTATGATTTAAGTATCACGTAGGCTCATCCTGAAAAAAAATAATAACAAATAATCTAAAAGCGTCGAAGCTTCTTAAc                                                                                                                                             >  2:604777/1‑150 (MQ=255)
         cGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACa                                                                                                                                            >  1:1013157/1‑150 (MQ=255)
            tGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAAc                                                                                                                                         <  2:243492/150‑1 (MQ=255)
              aGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTg                                                                                                                                       >  1:978282/1‑150 (MQ=255)
               ggCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgc                                                                                                                                      >  1:329930/1‑150 (MQ=255)
               ggCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgc                                                                                                                                      >  2:745625/1‑150 (MQ=255)
                gcgcgcGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcg                                                                                                                                     >  1:1229120/1‑150 (MQ=255)
                  gcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgcc                                                                                                                                   <  2:465568/150‑1 (MQ=255)
                  gcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgcc                                                                                                                                   <  1:1301994/150‑1 (MQ=255)
                  gcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                                                                   <  2:26434/150‑1 (MQ=255)
                   cgcgTCTCGCGCTTCTGGCGTGAGGTCCGTTGTGACCGTATCCGCGGCGGTACTGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgccg                                                                                                                                  <  2:817979/150‑1 (MQ=255)
                          cccGCTTCTGGCGTGATGTCCGTTGTTAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                                                                           >  1:924862/1‑150 (MQ=255)
                          cccGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                                                                           <  2:416961/150‑1 (MQ=255)
                          cccGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                                                                           <  1:1370173/150‑1 (MQ=255)
                                 cTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaa                                                                                                                    <  1:320236/150‑1 (MQ=255)
                                       tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccgc                                                                                                                                 <  1:581183/131‑1 (MQ=255)
                                       tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccgc                                                                                                                                 >  2:581183/1‑131 (MQ=255)
                                       tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac                                                                                                                   >  1:505062/1‑145 (MQ=255)
                                       tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac                                                                                                                   <  2:505062/145‑1 (MQ=255)
                                          tGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                                                                           >  2:465297/1‑150 (MQ=255)
                                          tGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                                                                           >  1:864333/1‑150 (MQ=255)
                                            tCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAcc                                                                                                         <  2:1229120/150‑1 (MQ=255)
                                              cGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCgg                                                                                                       <  2:329930/150‑1 (MQ=255)
                                                  gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                   >  2:1400352/1‑150 (MQ=255)
                                                     aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGa                                                                                                <  1:1380546/150‑1 (MQ=255)
                                                     aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGa                                                                                                >  2:1380546/1‑150 (MQ=255)
                                                        gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                                                                             <  1:125119/150‑1 (MQ=255)
                                                        gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                                                                             <  1:1175112/150‑1 (MQ=255)
                                                            cggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAg                                                                                         >  2:1144633/1‑150 (MQ=255)
                                                             ggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGa                                                                                        >  1:762662/1‑150 (MQ=255)
                                                                  cggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGa                                                                                   <  1:745625/150‑1 (MQ=255)
                                                                     tACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACATATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAttt                                                                                >  2:859191/1‑150 (MQ=255)
                                                                      aCAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAGCAAGCCTTACCGGTTGAGGAAATTCAGATAGGAtttg                                                                               >  2:209729/1‑149 (MQ=255)
                                                                       cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCt                                                                              <  1:1238615/150‑1 (MQ=255)
                                                                        aGACGAAATTATGATTTACGTAGCAGGTCGGCAGCTCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCtt                                                                             >  1:509821/1‑150 (MQ=255)
                                                                        aGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTACtt                                                                             >  2:618724/1‑150 (MQ=255)
                                                                         gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                                                                            >  2:924848/1‑150 (MQ=255)
                                                                         gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                                                                            <  1:423271/150‑1 (MQ=255)
                                                                          aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGt                                                                           <  2:924862/150‑1 (MQ=255)
                                                                             aaaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                                                                        <  1:1100828/150‑1 (MQ=255)
                                                                             aaaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                                                                        <  1:465297/150‑1 (MQ=255)
                                                                              aaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCg                                                                       >  1:1257748/1‑150 (MQ=255)
                                                                              aaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCg                                                                       <  1:1353468/150‑1 (MQ=255)
                                                                                ttATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCa                                                                     <  1:924848/150‑1 (MQ=255)
                                                                                    gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGa                                                                                   <  1:610071/132‑1 (MQ=255)
                                                                                    gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGa                                                                                   >  2:610071/1‑132 (MQ=255)
                                                                                    gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCaaaaa                                                                 >  2:1399480/1‑149 (MQ=255)
                                                                                    gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGACGCAAGATTTATCAAACAAGACTTACCGGATGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                                                                 >  2:847321/1‑150 (MQ=255)
                                                                                    gATTTACGTAGCAGGTCGACAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTAAACAAACTGCGCCGCCAGATTAATCCAACAAGAATTACCGGTAGAGGAAATTCAGATAGCATTTCTTGTTCCGCaaaaa                                                                 >  2:759275/1‑149 (MQ=255)
                                                                                       ttACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGt                                                              >  1:834005/1‑150 (MQ=255)
                                                                                        tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                             >  1:925266/1‑150 (MQ=255)
                                                                                        tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                             >  1:920406/1‑150 (MQ=255)
                                                                                          cGTAGCAGGTCGGCAGATACTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt                                                           >  2:440639/1‑150 (MQ=255)
                                                                                           gTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt                                                          >  1:1145808/1‑150 (MQ=255)
                                                                                            tAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGTAAAACAGTTTTTa                                                         <  1:1144633/150‑1 (MQ=255)
                                                                                            tAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTa                                                         >  1:1022042/1‑150 (MQ=255)
                                                                                               cAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAAt                                                      >  2:57789/1‑150 (MQ=255)
                                                                                                     ggCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTa                                                >  1:255141/1‑150 (MQ=255)
                                                                                                      gCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTAc                                               <  2:920406/150‑1 (MQ=255)
                                                                                                      gCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTAc                                               <  2:925266/150‑1 (MQ=255)
                                                                                                          aTCCTGAAAGTCTATCAGAACAAAAAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCATATTTATCCTACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                           <  2:1045070/150‑1 (MQ=255)
                                                                                                           tCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTaa                                          >  2:3089/1‑150 (MQ=255)
                                                                                                           tCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTaa                                          >  2:345640/1‑150 (MQ=255)
                                                                                                                aaaGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACGAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATAGTCTACGGTAATCGCt                                     >  1:1027825/1‑150 (MQ=255)
                                                                                                                 aaGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATATGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg                                    <  2:762662/150‑1 (MQ=255)
                                                                                                                    aCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTACTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGc                                 <  1:618724/150‑1 (MQ=255)
                                                                                                                    aCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGCTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGc                                 <  2:978282/150‑1 (MQ=255)
                                                                                                                     cTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCg                                <  1:209729/150‑1 (MQ=255)
                                                                                                                     cTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCg                                >  1:877785/1‑150 (MQ=255)
                                                                                                                        tCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaac                             <  2:476985/150‑1 (MQ=255)
                                                                                                                          aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTATTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATAGCTGGGCGaacaa                           >  2:300147/1‑150 (MQ=255)
                                                                                                                           gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaacaac                          >  2:1184157/1‑150 (MQ=255)
                                                                                                                               aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGAc                      <  2:834005/150‑1 (MQ=255)
                                                                                                                               aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGAc                      <  2:690003/150‑1 (MQ=255)
                                                                                                                                aataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACa                     <  1:440639/150‑1 (MQ=255)
                                                                                                                                 ataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACAt                    >  1:469924/1‑150 (MQ=255)
                                                                                                                                  taatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATg                   >  2:426486/1‑150 (MQ=255)
                                                                                                                                   aatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATgg                  <  1:57789/150‑1 (MQ=255)
                                                                                                                                       tGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCa              >  1:1220278/1‑150 (MQ=255)
                                                                                                                                        gCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaa             >  2:230089/1‑150 (MQ=255)
                                                                                                                                          aGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaa           >  2:380818/1‑150 (MQ=255)
                                                                                                                                           ggcggcGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaaa          >  1:1398639/1‑150 (MQ=255)
                                                                                                                                           ggcggcGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaaa          >  1:731272/1‑150 (MQ=255)
                                                                                                                                              ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg       >  2:41782/1‑150 (MQ=255)
                                                                                                                                              ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg       >  2:1102571/1‑150 (MQ=255)
                                                                                                                                                 gcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt    >  1:318586/1‑150 (MQ=255)
                                                                                                                                                 gcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt    <  2:864333/150‑1 (MQ=255)
                                                                                                                                                  cAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTg   <  1:1250774/150‑1 (MQ=255)
                                                                                                                                                  cAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTg   >  2:1096316/1‑150 (MQ=255)
                                                                                                                                                   aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg  >  1:460603/1‑150 (MQ=255)
                                                                                                                                                   aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg  >  1:465456/1‑150 (MQ=255)
                                                                                                                                                   aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg  <  1:1302709/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGG  >  NC_000913/1778215‑1778511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑