Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,966,368 G→A 7.4% intergenic (‑177/+25) flhB ← / ← cheZ flagellar biosynthesis protein FlhB/chemotaxis protein CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,966,3680GA 7.4% 164.3 / 2.8 54intergenic (‑177/+25)flhB/cheZflagellar biosynthesis protein FlhB/chemotaxis protein CheZ
Reads supporting (aligned to +/- strand):  ref base G (21/29);  new base A (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

CGTGAATGATGCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTC  >  NC_000913/1966219‑1966515
                                                                                                                                                     |                                                                                                                                                   
cGTGAATGATGCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTgg                                                                                                                                                     >  1:1121073/1‑150 (MQ=255)
      tgatgCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGc                                                                                                                                               >  1:830832/1‑150 (MQ=255)
             agagCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTTGTCACGCCACATCa                                                                                                                                        <  2:539254/150‑1 (MQ=255)
              gagCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAg                                                                                                                                       <  1:389002/150‑1 (MQ=255)
                  gcAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATGTGGCGTGACCACGTCATATCAGGcgg                                                                                                                                   <  1:141151/150‑3 (MQ=255)
                   cAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATGTGGCGTGACCACGTCATATCAGGcggt                                                                                                                                  <  2:573527/150‑4 (MQ=255)
                   cAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAAt                                                                                                                                  <  1:712902/150‑1 (MQ=255)
                    aaaGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATa                                                                                                                                 <  2:167161/150‑1 (MQ=255)
                        cGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACaaa                                                                                                                             <  2:1324697/150‑1 (MQ=255)
                          ttCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATc                                                                                                                           <  2:51894/150‑1 (MQ=255)
                                  tttGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCa                                                                                                                   >  2:1168241/1‑150 (MQ=255)
                                    tGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAg                                                                                                                 <  2:1091380/150‑1 (MQ=255)
                                     gAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGa                                                                                                                <  1:651835/150‑1 (MQ=255)
                                        tAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTa                                                                                                             >  2:1357750/1‑150 (MQ=255)
                                        tAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTa                                                                                                             <  2:196515/150‑1 (MQ=255)
                                          aGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACAAATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATc                                                                                                           >  2:891827/1‑150 (MQ=255)
                                             ggCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCcaa                                                                                                        >  1:1299520/1‑150 (MQ=255)
                                                    cAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCg                                                                                                 <  1:1357750/150‑1 (MQ=255)
                                                       gCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTcc                                                                                              <  1:1065326/150‑1 (MQ=255)
                                                         gggATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCAc                                                                                            >  1:10718/1‑150 (MQ=255)
                                                         gggATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCAc                                                                                            <  2:769184/150‑1 (MQ=255)
                                                               tAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATc                                                                                      <  2:185934/150‑1 (MQ=255)
                                                                aGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTTTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTAACCAACAAATCGTACACCTGATcc                                                                                     >  2:1093408/1‑150 (MQ=255)
                                                                 gggCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCcac                                                                                                                                            <  2:608837/94‑1 (MQ=255)
                                                                 gggCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCcac                                                                                                                                            >  1:608837/1‑94 (MQ=255)
                                                                   gCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACAATCCAACAAATCGTCCACCTGATCCTGa                                                                                  >  2:719493/1‑150 (MQ=255)
                                                                         gctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCt                                                                            >  2:1282681/1‑150 (MQ=255)
                                                                          ctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTa                                                                           <  2:566362/150‑1 (MQ=255)
                                                                          ctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTa                                                                           <  2:143683/150‑1 (MQ=255)
                                                                                 acaTCAAGCATGGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACcacaac                                                                    >  2:1180266/1‑148 (MQ=255)
                                                                                     cAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCt                                                                >  1:806307/1‑150 (MQ=255)
                                                                                      aaGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCtt                                                               >  2:524729/1‑150 (MQ=255)
                                                                                          aTAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCt                                                           >  1:1299432/1‑150 (MQ=255)
                                                                                            aGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTgg                                                         <  1:1368164/150‑1 (MQ=255)
                                                                                              ggtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTa                                                       <  2:778096/149‑1 (MQ=255)
                                                                                               gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                      >  2:1189253/1‑150 (MQ=255)
                                                                                               gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                      >  2:1184287/1‑150 (MQ=255)
                                                                                               gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                      <  1:1168241/150‑1 (MQ=255)
                                                                                                    tttGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGAcc                                                 <  1:1189253/150‑1 (MQ=255)
                                                                                                    tttGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGAcc                                                 <  1:1184287/150‑1 (MQ=255)
                                                                                                      tGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTg                                               >  1:532155/1‑150 (MQ=255)
                                                                                                      tGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTg                                               <  2:288389/150‑1 (MQ=255)
                                                                                                         cGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAgg                                            >  2:773522/1‑150 (MQ=255)
                                                                                                                   gTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGc                                  <  2:996492/150‑1 (MQ=255)
                                                                                                                          ccTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAActct                           <  1:524729/150‑1 (MQ=255)
                                                                                                                                 gtattgCCTGATGTGGCGTGACCACGTCATATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTc                    >  1:202840/6‑150 (MQ=38)
                                                                                                                                 aGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTc                    <  2:10718/150‑1 (MQ=255)
                                                                                                                                  gACCGCCTGATATGACGTTGTAACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCAAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCa                   <  1:746232/150‑1 (MQ=255)
                                                                                                                                   attgCCTGATGTGGCGTGACCACGTCATATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCAc                  >  1:861165/4‑150 (MQ=255)
                                                                                                                                         tGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTg            <  1:773522/150‑1 (MQ=255)
                                                                                                                                         tGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAACAAACTCTGGTTTTCACGTTTTg            <  2:1100365/150‑1 (MQ=255)
                                                                                                                                          gATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTgg           <  2:532155/150‑1 (MQ=255)
                                                                                                                                          gATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTgg           >  2:190696/1‑150 (MQ=255)
                                                                                                                                               gACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGAcgcg      >  1:587494/1‑150 (MQ=255)
                                                                                                                                                   tGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTc  >  2:374692/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGTGAATGATGCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTC  >  NC_000913/1966219‑1966515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑