Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,966,374 C→T 7.4% intergenic (‑183/+19) flhB ← / ← cheZ flagellar biosynthesis protein FlhB/chemotaxis protein CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,966,3740CT 7.4% 159.5 / 2.8 54intergenic (‑183/+19)flhB/cheZflagellar biosynthesis protein FlhB/chemotaxis protein CheZ
Reads supporting (aligned to +/- strand):  ref base C (21/29);  new base T (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

TGATGCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGT  >  NC_000913/1966225‑1966519
                                                                                                                                                     |                                                                                                                                                 
tgatgCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGc                                                                                                                                                   >  1:830832/1‑150 (MQ=255)
       agagCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTTGTCACGCCACATCa                                                                                                                                            <  2:539254/150‑1 (MQ=255)
        gagCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAg                                                                                                                                           <  1:389002/150‑1 (MQ=255)
            gcAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATGTGGCGTGACCACGTCATATCAGGcgg                                                                                                                                       <  1:141151/150‑3 (MQ=255)
             cAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATGTGGCGTGACCACGTCATATCAGGcggt                                                                                                                                      <  2:573527/150‑4 (MQ=255)
             cAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAAt                                                                                                                                      <  1:712902/150‑1 (MQ=255)
              aaaGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATa                                                                                                                                     <  2:167161/150‑1 (MQ=255)
                  cGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACaaa                                                                                                                                 <  2:1324697/150‑1 (MQ=255)
                    ttCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATc                                                                                                                               <  2:51894/150‑1 (MQ=255)
                            tttGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCa                                                                                                                       >  2:1168241/1‑150 (MQ=255)
                              tGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAg                                                                                                                     <  2:1091380/150‑1 (MQ=255)
                               gAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGa                                                                                                                    <  1:651835/150‑1 (MQ=255)
                                  tAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTa                                                                                                                 <  2:196515/150‑1 (MQ=255)
                                  tAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTa                                                                                                                 >  2:1357750/1‑150 (MQ=255)
                                    aGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACAAATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATc                                                                                                               >  2:891827/1‑150 (MQ=255)
                                       ggCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCcaa                                                                                                            >  1:1299520/1‑150 (MQ=255)
                                              cAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCg                                                                                                     <  1:1357750/150‑1 (MQ=255)
                                                 gCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTcc                                                                                                  <  1:1065326/150‑1 (MQ=255)
                                                   gggATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCAc                                                                                                >  1:10718/1‑150 (MQ=255)
                                                   gggATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCAc                                                                                                <  2:769184/150‑1 (MQ=255)
                                                         tAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATc                                                                                          <  2:185934/150‑1 (MQ=255)
                                                          aGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTTTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTAACCAACAAATCGTACACCTGATcc                                                                                         >  2:1093408/1‑150 (MQ=255)
                                                           gggCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCcac                                                                                                                                                <  2:608837/94‑1 (MQ=255)
                                                           gggCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCcac                                                                                                                                                >  1:608837/1‑94 (MQ=255)
                                                             gCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACAATCCAACAAATCGTCCACCTGATCCTGa                                                                                      >  2:719493/1‑150 (MQ=255)
                                                                   gctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCt                                                                                >  2:1282681/1‑150 (MQ=255)
                                                                    ctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTa                                                                               <  2:143683/150‑1 (MQ=255)
                                                                    ctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTa                                                                               <  2:566362/150‑1 (MQ=255)
                                                                           acaTCAAGCATGGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACcacaac                                                                        >  2:1180266/1‑148 (MQ=255)
                                                                               cAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCt                                                                    >  1:806307/1‑150 (MQ=255)
                                                                                aaGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCtt                                                                   >  2:524729/1‑150 (MQ=255)
                                                                                    aTAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCt                                                               >  1:1299432/1‑150 (MQ=255)
                                                                                      aGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTgg                                                             <  1:1368164/150‑1 (MQ=255)
                                                                                        ggtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTa                                                           <  2:778096/149‑1 (MQ=255)
                                                                                         gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                          >  2:1184287/1‑150 (MQ=255)
                                                                                         gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                          <  1:1168241/150‑1 (MQ=255)
                                                                                         gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                          >  2:1189253/1‑150 (MQ=255)
                                                                                              tttGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGAcc                                                     <  1:1184287/150‑1 (MQ=255)
                                                                                              tttGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGAcc                                                     <  1:1189253/150‑1 (MQ=255)
                                                                                                tGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTg                                                   <  2:288389/150‑1 (MQ=255)
                                                                                                tGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTg                                                   >  1:532155/1‑150 (MQ=255)
                                                                                                   cGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAgg                                                >  2:773522/1‑150 (MQ=255)
                                                                                                             gTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGc                                      <  2:996492/150‑1 (MQ=255)
                                                                                                                    ccTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAActct                               <  1:524729/150‑1 (MQ=255)
                                                                                                                           gtattgCCTGATGTGGCGTGACCACGTCATATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTc                        >  1:202840/6‑150 (MQ=38)
                                                                                                                           aGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTc                        <  2:10718/150‑1 (MQ=255)
                                                                                                                            gACCGCCTGATATGACGTTGTAACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCAAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCa                       <  1:746232/150‑1 (MQ=255)
                                                                                                                             attgCCTGATGTGGCGTGACCACGTCATATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCAc                      >  1:861165/4‑150 (MQ=255)
                                                                                                                                   tGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTg                <  1:773522/150‑1 (MQ=255)
                                                                                                                                   tGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAACAAACTCTGGTTTTCACGTTTTg                <  2:1100365/150‑1 (MQ=255)
                                                                                                                                    gATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTgg               <  2:532155/150‑1 (MQ=255)
                                                                                                                                    gATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTgg               >  2:190696/1‑150 (MQ=255)
                                                                                                                                         gACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGAcgcg          >  1:587494/1‑150 (MQ=255)
                                                                                                                                             tGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTc      >  2:374692/1‑150 (MQ=255)
                                                                                                                                                 cACGCCACATCAGGCAATAAAAATCAAAATCCAAGACTATCAAACAAATCGTCCACCTGATCCTGACTGGCTACCACAACGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGt  >  2:92086/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TGATGCCAGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGT  >  NC_000913/1966225‑1966519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑