Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,966,377 C→T 7.7% intergenic (‑186/+16) flhB ← / ← cheZ flagellar biosynthesis protein FlhB/chemotaxis protein CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,966,3770CT 7.7% 153.8 / 2.8 52intergenic (‑186/+16)flhB/cheZflagellar biosynthesis protein FlhB/chemotaxis protein CheZ
Reads supporting (aligned to +/- strand):  ref base C (20/28);  new base T (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

AGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGA  >  NC_000913/1966232‑1966526
                                                                                                                                                 |                                                                                                                                                     
agagCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTTGTCACGCCACATCa                                                                                                                                                   <  2:539254/150‑1 (MQ=255)
 gagCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAg                                                                                                                                                  <  1:389002/150‑1 (MQ=255)
     gcAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATGTGGCGTGACCACGTCATATCAGGcgg                                                                                                                                              <  1:141151/150‑3 (MQ=255)
      cAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATGTGGCGTGACCACGTCATATCAGGcggt                                                                                                                                             <  2:573527/150‑4 (MQ=255)
      cAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAAt                                                                                                                                             <  1:712902/150‑1 (MQ=255)
       aaaGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATa                                                                                                                                            <  2:167161/150‑1 (MQ=255)
           cGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACaaa                                                                                                                                        <  2:1324697/150‑1 (MQ=255)
             ttCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATc                                                                                                                                      <  2:51894/150‑1 (MQ=255)
                     tttGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCa                                                                                                                              >  2:1168241/1‑150 (MQ=255)
                       tGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAg                                                                                                                            <  2:1091380/150‑1 (MQ=255)
                        gAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGa                                                                                                                           <  1:651835/150‑1 (MQ=255)
                           tAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTa                                                                                                                        <  2:196515/150‑1 (MQ=255)
                           tAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTa                                                                                                                        >  2:1357750/1‑150 (MQ=255)
                             aGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACAAATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATc                                                                                                                      >  2:891827/1‑150 (MQ=255)
                                ggCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCcaa                                                                                                                   >  1:1299520/1‑150 (MQ=255)
                                       cAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCg                                                                                                            <  1:1357750/150‑1 (MQ=255)
                                          gCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTcc                                                                                                         <  1:1065326/150‑1 (MQ=255)
                                            gggATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCAc                                                                                                       <  2:769184/150‑1 (MQ=255)
                                            gggATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCAc                                                                                                       >  1:10718/1‑150 (MQ=255)
                                                  tAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATc                                                                                                 <  2:185934/150‑1 (MQ=255)
                                                   aGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTTTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTAACCAACAAATCGTACACCTGATcc                                                                                                >  2:1093408/1‑150 (MQ=255)
                                                    gggCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCcac                                                                                                                                                       <  2:608837/94‑1 (MQ=255)
                                                    gggCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCcac                                                                                                                                                       >  1:608837/1‑94 (MQ=255)
                                                      gCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACAATCCAACAAATCGTCCACCTGATCCTGa                                                                                             >  2:719493/1‑150 (MQ=255)
                                                            gctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCt                                                                                       >  2:1282681/1‑150 (MQ=255)
                                                             ctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTa                                                                                      <  2:566362/150‑1 (MQ=255)
                                                             ctgcCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTa                                                                                      <  2:143683/150‑1 (MQ=255)
                                                                    acaTCAAGCATGGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACcacaac                                                                               >  2:1180266/1‑148 (MQ=255)
                                                                        cAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCt                                                                           >  1:806307/1‑150 (MQ=255)
                                                                         aaGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCtt                                                                          >  2:524729/1‑150 (MQ=255)
                                                                             aTAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCt                                                                      >  1:1299432/1‑150 (MQ=255)
                                                                               aGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTgg                                                                    <  1:1368164/150‑1 (MQ=255)
                                                                                 ggtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTa                                                                  <  2:778096/149‑1 (MQ=255)
                                                                                  gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                                 >  2:1184287/1‑150 (MQ=255)
                                                                                  gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                                 >  2:1189253/1‑150 (MQ=255)
                                                                                  gtgCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTAt                                                                 <  1:1168241/150‑1 (MQ=255)
                                                                                       tttGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGAcc                                                            <  1:1189253/150‑1 (MQ=255)
                                                                                       tttGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGAcc                                                            <  1:1184287/150‑1 (MQ=255)
                                                                                         tGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTg                                                          >  1:532155/1‑150 (MQ=255)
                                                                                         tGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTg                                                          <  2:288389/150‑1 (MQ=255)
                                                                                            cGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAgg                                                       >  2:773522/1‑150 (MQ=255)
                                                                                                      gTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGc                                             <  2:996492/150‑1 (MQ=255)
                                                                                                             ccTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAActct                                      <  1:524729/150‑1 (MQ=255)
                                                                                                                    gtattgCCTGATGTGGCGTGACCACGTCATATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTc                               >  1:202840/6‑150 (MQ=38)
                                                                                                                    aGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTc                               <  2:10718/150‑1 (MQ=255)
                                                                                                                     gACCGCCTGATATGACGTTGTAACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCAAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCa                              <  1:746232/150‑1 (MQ=255)
                                                                                                                      attgCCTGATGTGGCGTGACCACGTCATATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCAc                             >  1:861165/4‑150 (MQ=255)
                                                                                                                            tGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTg                       <  1:773522/150‑1 (MQ=255)
                                                                                                                            tGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAACAAACTCTGGTTTTCACGTTTTg                       <  2:1100365/150‑1 (MQ=255)
                                                                                                                             gATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTgg                      <  2:532155/150‑1 (MQ=255)
                                                                                                                             gATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTgg                      >  2:190696/1‑150 (MQ=255)
                                                                                                                                  gACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGAcgcg                 >  1:587494/1‑150 (MQ=255)
                                                                                                                                      tGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTc             >  2:374692/1‑150 (MQ=255)
                                                                                                                                          cACGCCACATCAGGCAATAAAAATCAAAATCCAAGACTATCAAACAAATCGTCCACCTGATCCTGACTGGCTACCACAACGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGt         >  2:92086/1‑150 (MQ=255)
                                                                                                                                                acaTCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCggg   >  1:610041/1‑150 (MQ=255)
                                                                                                                                                 caTCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGa  <  1:891827/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AGAGCGCAAAGCGTTCAATGGTTTGAGTAAGGGGCAAAACAGGCGGGATTTAGGGCTTTTGCTGCCACACATCAAGCATAGTGTGCGTTTGTCGGATGCGGCGTCATCGCCTTATCAGACCGCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAATCAAAATCCAAGACTATCCAACAAATCGTCCACCTGATCCTGACTGGCTACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGA  >  NC_000913/1966232‑1966526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑