Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,992,083 G→C 5.2% noncoding (35/76 nt) glyW ← tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,992,0830GC 5.2% 260.7 / 4.4 77noncoding (35/76 nt)glyWtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (35/38);  new base C (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCA  >  NC_000913/1991934‑1992230
                                                                                                                                                     |                                                                                                                                                   
gAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                     >  1:529471/1‑150 (MQ=255)
gAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                     <  2:446662/150‑1 (MQ=255)
   ccGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCaa                                                                                                                                                  <  2:688542/150‑1 (MQ=255)
    cGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                                                                                                                                 >  2:660184/1‑150 (MQ=255)
          aGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                           >  1:860769/1‑150 (MQ=255)
          aGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                           >  1:1132030/1‑150 (MQ=255)
           gACGGATTTGCAGTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgggg                                                                                                                                          <  1:238845/150‑4 (MQ=255)
            aCGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct                                                                                                                                         <  2:715088/150‑1 (MQ=255)
            aCGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct                                                                                                                                         >  1:1257082/1‑150 (MQ=255)
             cGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                        >  2:641706/1‑150 (MQ=255)
                  ttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAACGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCa                                                                                                                                   >  2:720252/1‑150 (MQ=255)
                   tGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                                                                                  >  1:993325/1‑150 (MQ=255)
                     cAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                                >  1:26287/1‑150 (MQ=255)
                     cAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                                <  1:52197/150‑1 (MQ=255)
                      aaTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                               >  1:1071072/1‑150 (MQ=255)
                         ccGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc                                                                                                                            >  2:465351/1‑150 (MQ=255)
                               cATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTTGTGCTCTACCAACTGAGCTATTcc                                                                                                                      <  1:969234/150‑1 (MQ=255)
                               cATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                                                                                      <  1:429254/150‑1 (MQ=255)
                                aTAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                     >  1:211312/1‑150 (MQ=255)
                                 tAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCg                                                                                                                    >  1:1301054/1‑150 (MQ=255)
                                        tttGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                             >  2:919314/1‑150 (MQ=255)
                                            ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                         <  2:1097837/150‑1 (MQ=255)
                                            ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                         <  1:1046552/150‑1 (MQ=255)
                                             tAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGc                                                                                                        >  1:494335/1‑150 (MQ=255)
                                             tAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGc                                                                                                        <  1:660184/150‑1 (MQ=255)
                                              aaCGCCCCAAATTCTTCAGTCCTTTCAGCCTGTCATCCGCTAGACGCCGATGTCTTTTTAACTGGAGCGGGTATCGAGAGGCGTACTCGCGACCCCGACCTTGGCAATGTCGTGCTCTACCAACTGACCTATTCCCTAATTCATCAAGCa                                                                                                       <  2:558822/150‑1 (MQ=21)
                                                 gcgcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCAATTCCCGCATTCATCAAGCAATc                                                                                                    <  2:109291/150‑1 (MQ=255)
                                                 gcgcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAACGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATc                                                                                                    <  1:720252/150‑1 (MQ=255)
                                                  cgcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCa                                                                                                   <  1:641555/150‑1 (MQ=255)
                                                           cttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCAc                                                                                          >  1:1246052/1‑150 (MQ=255)
                                                            ttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACt                                                                                         <  2:993325/150‑1 (MQ=255)
                                                              cAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTg                                                                                       <  2:26287/150‑1 (MQ=255)
                                                                ggCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAt                                                                                     >  2:289140/1‑150 (MQ=255)
                                                                ggCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAt                                                                                     >  2:11761/1‑150 (MQ=255)
                                                                     ttCAGCCAGACTTCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCTAACTCGAGACCCCGACCTTTGCAAGGTCGTGCTCTACCTACTGAGGTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTttat                                                                                <  1:1364234/150‑1 (MQ=37)
                                                                       cAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTttatta                                                                              <  1:465758/150‑1 (MQ=255)
                                                                           cAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGt                                                                          <  1:396104/150‑1 (MQ=255)
                                                                                  ccGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCaa                                                                   >  2:173566/1‑150 (MQ=255)
                                                                                  ccGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCaa                                                                   >  2:1419239/1‑150 (MQ=255)
                                                                                   cGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAAt                                                                  <  2:469542/150‑1 (MQ=255)
                                                                                      ttGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAg                                                               <  1:1202521/150‑1 (MQ=255)
                                                                                       tGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGt                                                              >  2:6788/1‑150 (MQ=255)
                                                                                        gACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTg                                                             <  1:740407/150‑1 (MQ=255)
                                                                                        gACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCT‑GCAATCAGTgc                                                            >  2:338580/1‑150 (MQ=255)
                                                                                           gCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccg                                                          <  2:211312/150‑1 (MQ=255)
                                                                                           gCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccg                                                          <  1:381246/150‑1 (MQ=255)
                                                                                            ccGATGTCTTTTAAACTGGAGCGTGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccgc                                                         <  2:646955/150‑1 (MQ=255)
                                                                                               aTGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGt                                                      >  2:990396/1‑150 (MQ=255)
                                                                                                  tCTTTTAAACTGGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGGCGTTCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCg                                                   <  1:713982/150‑1 (MQ=255)
                                                                                                   cTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGa                                                  >  2:474444/1‑150 (MQ=255)
                                                                                                         aaCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGtt                                            >  2:569048/1‑150 (MQ=255)
                                                                                                         aaCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGtt                                            <  1:641706/150‑1 (MQ=255)
                                                                                                          aCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTg                                           >  1:1427149/1‑150 (MQ=255)
                                                                                                           cTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGc                                          >  2:966176/1‑150 (MQ=255)
                                                                                                           cTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGc                                          <  2:726797/150‑1 (MQ=255)
                                                                                                                gCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCt                                     <  2:1243396/150‑1 (MQ=255)
                                                                                                                  gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                   <  1:990396/150‑1 (MQ=255)
                                                                                                                  gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                   >  1:856728/1‑150 (MQ=255)
                                                                                                                  gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                   <  2:856728/150‑1 (MQ=255)
                                                                                                                   ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACt                                  >  1:349977/1‑150 (MQ=255)
                                                                                                                      aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTAc                               <  2:1422788/150‑1 (MQ=255)
                                                                                                                             cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGAGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGgcgc                        <  1:851820/150‑1 (MQ=255)
                                                                                                                              tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGcgcg                       <  2:494335/150‑1 (MQ=255)
                                                                                                                              tCGAAATCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGATCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCTATGCGTTGCATTCTACTTACCTTGcgcg                       <  2:1301175/150‑1 (MQ=255)
                                                                                                                               cGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGa                      >  2:171748/1‑150 (MQ=255)
                                                                                                                                gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGAt                     >  1:1329233/1‑150 (MQ=255)
                                                                                                                                 aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATg                    <  2:1257082/150‑1 (MQ=255)
                                                                                                                                 aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATg                    >  2:1096636/1‑150 (MQ=255)
                                                                                                                                  aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGa                   >  1:753517/1‑150 (MQ=255)
                                                                                                                                   cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAg                  <  1:867933/150‑1 (MQ=255)
                                                                                                                                     cgcgACCCCGACCTTGGCAAGGTAGTGCTCTACCAACTGAGCTATTCCCGCATTAATCAAGAAATCATTTAATCACTTGATTTTATTATCTTCTGGAAATCAGTGCCGCAGTTCGATGCGTTTCATTATACTTACCTGGCGCGATGAGTc                >  2:212611/1‑150 (MQ=37)
                                                                                                                                       cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGTCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCaa              >  2:532757/1‑150 (MQ=255)
                                                                                                                                       cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCaa              <  1:11761/150‑1 (MQ=255)
                                                                                                                                          gcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGa           >  2:1334886/4‑150 (MQ=255)
                                                                                                                                           cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                   <  2:604605/123‑1 (MQ=255)
                                                                                                                                           cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                   >  1:604605/3‑125 (MQ=255)
                                                                                                                                            ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGata         >  2:1359457/1‑150 (MQ=255)
                                                                                                                                               aCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATAttt      <  2:1071072/150‑1 (MQ=255)
                                                                                                                                                   tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca  <  1:919314/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCA  >  NC_000913/1991934‑1992230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑