| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,151,969 | T→A | 7.8% | R168S (AGA→AGT) | pphC ← | protein‑serine/threonine phosphatase PphC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,969 | 0 | T | A | 7.8% | 176.8 / 3.8 | 59 | R168S (AGA→AGT) | pphC | protein‑serine/threonine phosphatase PphC |
| Reads supporting (aligned to +/- strand): ref base T (27/27); new base A (2/3); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
TGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGA > NC_000913/2151823‑2152118 | tGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTg > 2:308356/1‑150 (MQ=255) ctgcCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGATGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACgg < 1:419195/150‑1 (MQ=255) gccgccAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGACTGGAAActttc < 2:127350/150‑5 (MQ=255) ccgccAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGACTGGAAActttca < 2:119626/150‑6 (MQ=255) gccAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCt > 2:988391/1‑150 (MQ=255) gccAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGACTGGAAActttcacc < 2:1220839/150‑8 (MQ=255) aGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTtcg < 2:667556/150‑1 (MQ=255) tCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTtcgtc < 1:995586/150‑1 (MQ=255) aTTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGt > 2:1026214/1‑150 (MQ=255) gAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAAt > 2:1329953/1‑150 (MQ=255) gAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGAATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAAt < 2:1391469/150‑1 (MQ=255) aaaaaCGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAAc < 2:998567/131‑1 (MQ=255) aaaaaCGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAAc > 1:998567/1‑131 (MQ=255) ggggTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGc > 1:833361/1‑150 (MQ=255) ggTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGt > 1:408633/1‑150 (MQ=255) tAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCa > 1:121487/1‑150 (MQ=255) aagGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCg < 1:439459/141‑1 (MQ=255) aagGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCg > 2:439459/1‑141 (MQ=255) agGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGc < 2:121487/150‑1 (MQ=255) ggCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCa < 2:875073/150‑1 (MQ=255) ggCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCa < 2:1049736/150‑1 (MQ=255) cacaTGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCata > 1:309188/1‑150 (MQ=255) caTGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTCCTGGTGAAACTTTCCAGACTGGAAACGGCAACTTCCACGCTAATGAAGTGCCTCATCTACGCatata > 2:449044/1‑149 (MQ=255) caTGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATCCTGCAACATTGTGCGCACGCCCAGTGATGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATAtt > 1:1132770/1‑150 (MQ=255) tGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCa > 2:745546/1‑150 (MQ=255) aTCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCgg > 1:541388/1‑150 (MQ=255) aGCATATTTAACGCCTGTCGCTGGATAGCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCTTCGGTAATGAAGTGCGTCATTTTGGCATATTCACCCGCCATCGGCGTg < 2:1132770/150‑1 (MQ=255) aGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCg < 1:188879/118‑1 (MQ=255) aGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCg > 2:188879/1‑118 (MQ=255) cATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTgag < 2:309188/150‑1 (MQ=255) ttAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCa < 1:744027/150‑1 (MQ=255) gCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTCCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGc < 1:439150/150‑1 (MQ=255) gTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGAc < 1:745546/150‑1 (MQ=255) gCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGt > 2:884501/1‑150 (MQ=255) cTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTg < 2:207789/150‑1 (MQ=255) ggATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCg < 1:272245/146‑1 (MQ=255) ggATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCg > 2:272245/1‑146 (MQ=255) ccATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCaaa > 1:572597/1‑150 (MQ=255) ccATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACAGTGACCCaaa > 1:770010/1‑150 (MQ=255) cATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAAt > 1:243839/1‑150 (MQ=255) aTCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGTCAATTGCAGACCGTGACCCAAATc > 1:946690/1‑150 (MQ=255) aTCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATc > 1:1283741/1‑150 (MQ=255) cGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTac < 2:60128/150‑1 (MQ=255) cGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGGCATGGTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTac < 2:596922/150‑1 (MQ=255) gTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTacc < 1:175218/150‑1 (MQ=255) gctgcAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCAcg > 1:418358/1‑150 (MQ=255) aaCTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACgccgcc < 2:842541/150‑1 (MQ=255) tttGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATc > 2:879973/1‑150 (MQ=255) gtgCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGcc < 1:1329953/150‑1 (MQ=255) gtgCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGcc < 1:54890/150‑1 (MQ=255) cgcACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAAt > 1:555367/1‑150 (MQ=255) gcACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATc > 1:764696/1‑150 (MQ=255) gccgTTTCCAGACTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCg > 1:1329372/4‑150 (MQ=255) gTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCa < 1:928763/150‑1 (MQ=255) tttCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAg < 1:308356/150‑1 (MQ=255) ttCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAgg < 2:541388/150‑1 (MQ=255) cAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGa > 1:578996/1‑150 (MQ=255) cAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGa > 2:251312/1‑150 (MQ=255) gTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATg < 2:555367/150‑1 (MQ=255) tCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGa < 2:833361/150‑1 (MQ=255) | TGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTCGTCGGTAATGAAGTGCGTCATGTTGGCATATTCACCCGCCATCGGCGTGAGAGGCAATTGCAGACCGTGACCCAAATCTACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGA > NC_000913/2151823‑2152118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |