Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,376,935 G→A 6.2% L220L (CTG→TTG) ‡ menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,376,9350GA 6.2% 200.1 / 2.4 64L220L (CTG→TTG) ‡menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base A (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

CGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCATTATTG  >  NC_000913/2376789‑2377083
                                                                                                                                                  |                                                                                                                                                    
cGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTc                                                                                                                                                   >  1:1119121/1‑150 (MQ=255)
  tAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGACAGACTTGCGATTACGCCAGCTGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTccc                                                                                                                                                 >  1:528608/1‑149 (MQ=255)
    aaGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTccgcc                                                                                                                                               >  1:782628/1‑150 (MQ=255)
           gCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAggg                                                                                                                                        >  1:1300542/1‑150 (MQ=255)
                atcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCgg                                                                                                                                   <  2:963213/150‑1 (MQ=255)
                 tcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGa                                                                                                                                  <  2:760754/150‑1 (MQ=255)
                        aaaTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCt                                                                                                                           >  2:1084876/1‑150 (MQ=255)
                          aTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGt                                                                                                                         >  1:753233/1‑150 (MQ=255)
                          aTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGt                                                                                                                         >  2:757142/1‑150 (MQ=255)
                           tCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCAAATTCCGCGCCCTGGCGGCGGAACTGGCTgcc                                                                                                                        <  2:672598/150‑3 (MQ=38)
                           tCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCAAATTCCGCGCCCTGGCGGCGGAACTGGCTgcc                                                                                                                        <  2:675087/150‑3 (MQ=38)
                                     tCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcacca                                                                                                              >  1:1028436/1‑150 (MQ=255)
                                       cTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACcaca                                                                                                            <  1:1250370/150‑1 (MQ=255)
                                       cTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACcaca                                                                                                            >  2:1067569/1‑150 (MQ=255)
                                        tttAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACat                                                                                                           >  1:1290560/1‑150 (MQ=255)
                                           aGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaa                                                                                                        <  2:554237/150‑1 (MQ=255)
                                              cAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaataa                                                                                                     >  2:126930/1‑150 (MQ=255)
                                               aGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAaataat                                                                                                    <  2:1300542/150‑1 (MQ=255)
                                                gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAataata                                                                                                   <  2:856628/150‑1 (MQ=255)
                                                gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAataata                                                                                                   <  2:1247465/150‑1 (MQ=255)
                                                     gCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACg                                                                                              <  2:753233/150‑1 (MQ=255)
                                                                 ccAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCggg                                                                                  >  2:1277601/1‑150 (MQ=255)
                                                                 ccAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCggg                                                                                  <  1:1067569/150‑1 (MQ=255)
                                                                 ccAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCggg                                                                                  >  2:959696/1‑150 (MQ=255)
                                                                  cAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgc                                                                                 <  2:894399/150‑1 (MQ=255)
                                                                     tcTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCt                                                                              <  2:412012/149‑1 (MQ=255)
                                                                     aCTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCt                                                                              <  1:959696/150‑1 (MQ=255)
                                                                         gCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAgg                                                                          >  2:899703/1‑150 (MQ=255)
                                                                          cGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGt                                                                         >  2:280143/1‑150 (MQ=255)
                                                                          cGATTACGCCAGCGGGATTTACCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGt                                                                         >  2:821938/1‑150 (MQ=255)
                                                                             ttACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAg                                                                      <  1:280143/150‑1 (MQ=255)
                                                                               aCGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCa                                                                    >  1:1019280/1‑150 (MQ=255)
                                                                                cGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCAc                                                                   >  1:1071465/1‑150 (MQ=255)
                                                                                     gCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTaaa                                                              <  1:899703/150‑1 (MQ=255)
                                                                                        ggATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCa                                                           <  1:847666/150‑1 (MQ=255)
                                                                                           ttttCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGc                                                        <  1:1428528/150‑1 (MQ=255)
                                                                                             ttCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCg                                                                                    <  1:1408768/120‑1 (MQ=255)
                                                                                             ttCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCg                                                                                    >  2:1408768/1‑120 (MQ=255)
                                                                                                   aTGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACggg                                                <  1:330081/150‑2 (MQ=255)
                                                                                                     gcgcGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCga                                              >  2:515319/1‑150 (MQ=255)
                                                                                                      cgcgTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCgag                                             <  2:1145890/150‑1 (MQ=255)
                                                                                                       gcgTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCgaga                                            >  1:710701/1‑150 (MQ=255)
                                                                                                       gcgTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCgaga                                            >  1:632505/1‑150 (MQ=255)
                                                                                                           tATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAg                                        <  1:757142/150‑1 (MQ=255)
                                                                                                            aTGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGt                                       >  1:356318/1‑150 (MQ=255)
                                                                                                             tGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTc                                      >  1:868948/1‑150 (MQ=255)
                                                                                                                ccGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGcc                                   <  1:1038472/150‑1 (MQ=255)
                                                                                                                  ggCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTc                                 >  1:1308369/1‑150 (MQ=255)
                                                                                                                        aGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCgaga                                            >  1:1020682/1‑133 (MQ=255)
                                                                                                                        aGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCgaga                                            <  2:1020682/133‑1 (MQ=255)
                                                                                                                          gAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATgg                         >  1:1196834/1‑150 (MQ=255)
                                                                                                                              gACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATggcggc                     <  2:782628/150‑1 (MQ=255)
                                                                                                                              gACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATggcggc                     <  1:126930/150‑1 (MQ=255)
                                                                                                                                  tGGCAGTCGGCAGCCAGTTCCGCCGCCAGTGCGCGTAATTTGCTGTCACGTTCACGACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTATATCAGGCTGGACGGCGAGAGAAGTCTCCTCCAGCATGGCGGCAAGc                 <  2:528608/150‑1 (MQ=255)
                                                                                                                                  tGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGc                 >  2:404577/1‑150 (MQ=255)
                                                                                                                                     cAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGtt              >  2:971038/1‑150 (MQ=255)
                                                                                                                                       gTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTgc            <  1:1277601/150‑1 (MQ=255)
                                                                                                                                        gtgacAGCAAATTCCGCGCCCTGGCGGCGGAACTGGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTgcg           >  1:197366/5‑150 (MQ=38)
                                                                                                                                         cGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTgcgc          >  1:317271/1‑150 (MQ=255)
                                                                                                                                            cAGCAAATTCCGCGCCCTGGCGGCGGAACTGGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCat       >  1:244102/1‑150 (MQ=255)
                                                                                                                                             aGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCatt      <  2:1063048/150‑1 (MQ=255)
                                                                                                                                              gCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCatta     <  2:1257549/150‑1 (MQ=255)
                                                                                                                                               ccAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCattat    >  1:7223/1‑150 (MQ=255)
                                                                                                                                                 aGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCATTAttg  <  2:868948/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTGGACGGCGAGAGAAGTCGCCTCCAGCATGGCGGCAAGCGTTGCGCCATTATTG  >  NC_000913/2376789‑2377083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑