Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,441,661 C→A 9.0% intergenic (‑56/‑103) fabB ← / → mnmC beta‑ketoacyl‑[acyl carrier protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,441,6610CA 9.0% 176.7 / 4.5 56intergenic (‑56/‑103)fabB/mnmCbeta‑ketoacyl‑[acyl carrier protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base A (3/3);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGA  >  NC_000913/2441517‑2441810
                                                                                                                                                |                                                                                                                                                     
cGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTa                                                                                                                                                  >  1:1011267/1‑150 (MQ=255)
   ccTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGc                                                                                                                                               >  2:1081392/1‑150 (MQ=255)
    cTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGcc                                                                                                                                              <  1:955723/150‑1 (MQ=255)
      tCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctat                                                                                                                                            <  1:739236/150‑4 (MQ=255)
       cACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctatt                                                                                                                                           <  2:70887/150‑5 (MQ=255)
       cACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctatt                                                                                                                                           <  2:110094/150‑5 (MQ=255)
        aCGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAAc                                                                                                                                          >  1:1362263/1‑150 (MQ=255)
             gagaTGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTc                                                                                                                                     <  2:322175/150‑1 (MQ=255)
              agaTGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTcc                                                                                                                                    <  2:171649/150‑1 (MQ=255)
                   ccAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATcc                                                                                                                               >  2:1266637/1‑149 (MQ=255)
                     aGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGc                                                                                                                             >  2:100919/1‑150 (MQ=255)
                       gACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCa                                                                                                                           <  2:559791/150‑1 (MQ=255)
                           tCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTa                                                                                                                       >  2:700532/1‑150 (MQ=255)
                                 tgGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATagag                                                                                                                 <  1:398010/150‑1 (MQ=255)
                                  ggTTATTACCGATGATGGAAACAATGCACAGGTCAGTAATAACTGCACGTTTAATTCAATACCTCAGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAAAAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCAAATTAAAAgaga                                                                                                                >  2:1135955/1‑150 (MQ=255)
                                    ttattaCCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGaaa                                                                                                              <  1:409861/150‑1 (MQ=255)
                                     tattaCCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAAt                                                                                                             <  2:495482/150‑1 (MQ=255)
                                      attaCCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAAtt                                                                                                            <  2:1011267/150‑1 (MQ=255)
                                              tGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGACTACAATTGTAAGCCGAACAAGTACGATAAGACAATTAATAGAGAAATTTgataaga                                                                                                    >  2:976804/1‑144 (MQ=255)
                                                cTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCt                                                                                                  >  1:232452/1‑150 (MQ=255)
                                                     aaCAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTacac                                                                                             <  1:100919/150‑1 (MQ=255)
                                                     aaCAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTacac                                                                                             <  2:742605/150‑1 (MQ=255)
                                                          tGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATc                                                                                        >  1:1032249/1‑150 (MQ=255)
                                                                    aGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCg                                                                              <  1:958045/150‑1 (MQ=255)
                                                                            cTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGCACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCg                                                                      <  2:209563/150‑1 (MQ=255)
                                                                             tGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCAAAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGc                                                                     >  1:19074/1‑150 (MQ=255)
                                                                               cACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCt                                                                   <  2:589064/150‑1 (MQ=255)
                                                                                aCGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTg                                                                  <  2:51584/150‑1 (MQ=255)
                                                                                  gTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTa                                                                <  2:1236561/150‑1 (MQ=255)
                                                                                   tttCATTCAATAGCTCTGTAACTCGCACATAGAGTAAGTTTCGAATTCACAATAGGGTAAACATGTAGGCCCAACAAGGCCGCTCTGACATTAAATAGGGAAATTTGCGCCGGATTACACACTGCGCAAAGATCGAGCCAGCGCCGGtgg                                                               >  2:1156053/1‑148 (MQ=255)
                                                                                            aTACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTaa                                                      >  2:652044/1‑150 (MQ=255)
                                                                                              aCCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACTCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACt                                                    <  2:73575/150‑1 (MQ=255)
                                                                                              aCCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACt                                                    >  2:1109939/1‑150 (MQ=255)
                                                                                               ccTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACtt                                                   >  1:638005/1‑150 (MQ=255)
                                                                                                ctctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAAc                                                     >  2:18566/1‑147 (MQ=255)
                                                                                                ctctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAAc                                                     <  1:18566/147‑1 (MQ=255)
                                                                                                      aaGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGaa                                            <  2:1016491/150‑1 (MQ=255)
                                                                                                        gTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAac                                          <  1:1033096/150‑1 (MQ=255)
                                                                                                        gTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAac                                          <  2:517174/150‑1 (MQ=255)
                                                                                                        gTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGACACCGCCTGTAAGACGAGTAACTTACGTGAAac                                          <  2:1370302/150‑1 (MQ=255)
                                                                                                            cacaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACacta                                      >  2:733857/1‑150 (MQ=255)
                                                                                                                  gagTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCAt                                >  1:27109/1‑150 (MQ=255)
                                                                                                                   agTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATa                               >  2:935661/1‑150 (MQ=255)
                                                                                                                    gTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATAc                              >  2:967838/1‑150 (MQ=255)
                                                                                                                         tttCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCt                         >  1:1069518/1‑150 (MQ=255)
                                                                                                                         tttCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCt                         >  1:277055/1‑150 (MQ=255)
                                                                                                                          ttCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTg                        >  1:737425/1‑150 (MQ=255)
                                                                                                                                    caATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGa              >  1:718478/1‑150 (MQ=255)
                                                                                                                                     ttcggCGTACAAGTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGaa             >  1:257725/5‑150 (MQ=255)
                                                                                                                                     aaTAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGaa             >  1:1028570/1‑150 (MQ=255)
                                                                                                                                      tcggCGTACAACTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAt            >  2:1038821/4‑150 (MQ=255)
                                                                                                                                       tAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAtt           <  2:19074/150‑1 (MQ=255)
                                                                                                                                       tAGAGTACACTTGTACGCCTAACAATTCCTATCAGCCATTTAATAGAGAAATTTTCTCAGCCTTACACACATCGCTAAGATCTAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTAGTCCATACAACCTGCCAACCTCGAAtt           <  1:94534/150‑1 (MQ=255)
                                                                                                                                        aGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAttt          >  1:107587/1‑150 (MQ=255)
                                                                                                                                        aGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAttt          <  2:1362263/150‑1 (MQ=255)
                                                                                                                                          cGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTaa        >  1:1350805/1‑150 (MQ=255)
                                                                                                                                             acacTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGc     <  1:1081392/150‑1 (MQ=255)
                                                                                                                                              cacTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt    >  1:63803/1‑150 (MQ=255)
                                                                                                                                              cacTTGTACGCCGAACAAGTCCGATAAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCAAACAACCTGCCAACCTCGAATTTAATGCt    >  1:1394612/1‑150 (MQ=255)
                                                                                                                                                cTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGa  >  2:508649/1‑150 (MQ=255)
                                                                                                                                                cTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATCTAATGCTGa  >  2:27443/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGA  >  NC_000913/2441517‑2441810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑