Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 247,476 T→A 9.1% intergenic (+15/‑161) yafL → / → rayT NlpC/P60 family protein YafL/REP‑associated tyrosine transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913247,4760TA 9.1% 195.9 / 8.7 66intergenic (+15/‑161)yafL/rayTNlpC/P60 family protein YafL/REP‑associated tyrosine transposase
Reads supporting (aligned to +/- strand):  ref base T (27/33);  new base A (2/4);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

ATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGA  >  NC_000913/247328‑247623
                                                                                                                                                    |                                                                                                                                                   
aTTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGttt                                                                                                                                                    >  2:1367018/1‑150 (MQ=255)
  ttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTAc                                                                                                                                                  >  1:307190/1‑150 (MQ=255)
   tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAATCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGTACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTAcg                                                                                                                                                 <  2:1320051/150‑1 (MQ=255)
       cGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTAcgccgc                                                                                                                                             <  1:104513/150‑1 (MQ=255)
        gATGGGCAATTTATCGACTCGCCCCGTACCGGCGAAACCATACGGATAAGCCGCTTAGCCGACCCTTTCTGGCAGTACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGCACGGAAAAGGCCTTTAcgccgcc                                                                                                                                            >  2:553109/1‑149 (MQ=255)
           gggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTGCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATGCGGCGTAAACGCCTTATcc                                                                                                                                         >  2:205219/1‑150 (MQ=255)
            ggCAATTTATCGAGTCTCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                        <  2:1309297/150‑1 (MQ=255)
             gCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                       >  1:1331397/1‑150 (MQ=255)
              cAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                      <  2:145942/150‑1 (MQ=255)
              cAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                      <  2:1027199/150‑1 (MQ=255)
                aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATGCGGCGTAAACGCCTTATCCgtcct                                                                                                                                    <  1:723883/150‑5 (MQ=255)
                aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                                                    <  2:4786/150‑1 (MQ=255)
                 tttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                                                   >  1:1278289/1‑150 (MQ=255)
                 tttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTCCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATGCGGCGTAAACGCCTTATCCgtccta                                                                                                                                   <  1:205219/150‑6 (MQ=255)
                     tCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                                               <  2:853966/150‑1 (MQ=255)
                     tCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                                               >  2:1201146/1‑150 (MQ=255)
                      cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAACAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTAc                                                                                                                              >  2:1367325/1‑150 (MQ=255)
                       gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                                             >  2:909819/1‑150 (MQ=255)
                           cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGTACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTCGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAgg                                                                                                                         >  1:243551/1‑150 (MQ=255)
                           cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAgg                                                                                                                         >  2:220674/1‑150 (MQ=255)
                                cGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGAGCATTTTTTGGTCGCGCGCATGATTTTGTCGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACTCCGCATGCGGCAGTTGTACGCAGGTGCCt                                                                                                                    <  2:560114/150‑1 (MQ=255)
                                  tACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                                                  <  1:909819/150‑1 (MQ=255)
                                     cGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                                                                               <  2:193773/150‑1 (MQ=255)
                                        cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGAc                                                                                                            >  2:74980/1‑150 (MQ=255)
                                        cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGAc                                                                                                            >  1:84209/1‑150 (MQ=255)
                                                 tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                                                   <  1:74980/150‑1 (MQ=255)
                                                  cGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                                                  >  1:1127838/1‑150 (MQ=255)
                                                   ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                                                 <  1:649946/150‑1 (MQ=255)
                                                   ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                                                 <  1:1297584/150‑1 (MQ=255)
                                                   ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                                                 <  2:1061562/150‑1 (MQ=255)
                                                    gattagccgattagccGTAACTTTCTGGCAGGACCA‑TTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                                               <  1:276280/150‑1 (MQ=255)
                                                      tAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                                              <  1:685885/150‑1 (MQ=255)
                                                        aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                            >  1:1238333/1‑150 (MQ=255)
                                                        aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                            >  1:286917/1‑150 (MQ=255)
                                                        aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                            >  2:1233054/1‑150 (MQ=255)
                                                         gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                           <  1:74266/150‑1 (MQ=255)
                                                            gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGc                                                                                        >  1:3616/1‑150 (MQ=255)
                                                                aGCCGAACCTTTCTGGCTGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                                                    <  1:791271/150‑1 (MQ=255)
                                                                 gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                                                   <  2:1325918/150‑1 (MQ=255)
                                                                          ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                                                          <  1:1367018/150‑1 (MQ=255)
                                                                                   gACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAACAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                                                 <  1:1367325/150‑1 (MQ=255)
                                                                                      cATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGc                                                              >  1:369899/1‑150 (MQ=255)
                                                                                                gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCg                                                    <  2:1355466/150‑1 (MQ=255)
                                                                                                 cgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGa                                                   >  2:446881/1‑150 (MQ=255)
                                                                                                 cgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGa                                                   <  2:1278289/150‑1 (MQ=255)
                                                                                                   cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGata                                                 <  1:1201146/150‑1 (MQ=255)
                                                                                                    gcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                                                <  2:1331397/150‑1 (MQ=255)
                                                                                                        aGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGTCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCTATATaaaa                                            <  1:897902/150‑1 (MQ=255)
                                                                                                         ggATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac                                           >  1:13638/1‑150 (MQ=255)
                                                                                                            ttttGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac                                        <  1:475685/150‑1 (MQ=255)
                                                                                                            ttttGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac                                        >  2:1414042/1‑150 (MQ=255)
                                                                                                                   ggAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg                                 <  1:446881/150‑1 (MQ=255)
                                                                                                                           cGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAcctt                         >  1:605367/1‑150 (MQ=255)
                                                                                                                            gATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAccttt                        <  2:286917/150‑1 (MQ=255)
                                                                                                                             aTTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAcctttc                       >  1:201817/1‑150 (MQ=255)
                                                                                                                             aTTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAcctttc                       <  2:307190/150‑1 (MQ=255)
                                                                                                                               tttGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAcctttcct                     <  1:1233054/150‑1 (MQ=255)
                                                                                                                                     ggACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTttctt               >  1:1378979/1‑150 (MQ=255)
                                                                                                                                      gccGGATGCGGCGTAAACGCCTTATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTttcttc              >  1:12249/3‑150 (MQ=38)
                                                                                                                                      gACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTttcttc              <  2:84209/150‑1 (MQ=255)
                                                                                                                                       ccGGATGCGGCGTAAACGCCTTATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTtcttct             <  2:12249/149‑1 (MQ=38)
                                                                                                                                          gATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTAc          >  2:403018/1‑150 (MQ=255)
                                                                                                                                          gATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTAc          <  2:1238333/150‑1 (MQ=255)
                                                                                                                                                 cGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTgag   <  2:1015940/150‑1 (MQ=255)
                                                                                                                                                 cGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTgag   >  1:875113/1‑150 (MQ=255)
                                                                                                                                                 cGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTgag   >  2:729743/1‑150 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTgaga  >  1:892044/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGA  >  NC_000913/247328‑247623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑