Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,623,776	G→A	8.1%	E245K (GAG→AAG)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,623,776	0	G	A	8.1%	143.1 / 2.9	49	E245K (GAG→AAG)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base A (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

CTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCG  >  NC_000913/2623635‑2623908                                                                                                                                              |                                                                                                                                     cttctGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATg                                                                                                                              <  1:6014/150‑1 (MQ=255)    ctGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGAAGTTGATGTCt                                                                                                                           >  2:1399740/1‑150 (MQ=255)      ggATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTc                                                                                                                         >  2:1122362/1‑150 (MQ=255)       gATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTcc                                                                                                                        <  1:646151/150‑1 (MQ=255)             aTTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGtctc                                                                                                                  >  1:868300/1‑150 (MQ=255)               tCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCaa                                                                                                                <  2:313549/150‑1 (MQ=255)                 tGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAagc                                                                                                              <  2:192603/150‑1 (MQ=255)                  gCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAagca                                                                                                             >  2:1427141/1‑150 (MQ=255)                       aCTGCGTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGtt                                                                                                        >  2:412079/1‑150 (MQ=255)                        cTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGttt                                                                                                       >  2:77956/1‑150 (MQ=255)                         tcccTTGATGATATTTTCAAAGGCTTCTTTCATTAAGACGCGCTGAATGCCTATTCAATGAAGATGACACGCGATGCCGAATACTAATTAGAGCAAGAGATGGAAGCCAGCAAGAACAACTTGATGTCTTCCAGTCTCAAGCAGCGTTTa                                                                                                      >  2:1383610/3‑150 (MQ=255)                          gCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTg                                                                                                                                 >  1:535250/1‑122 (MQ=255)                          gCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTg                                                                                                                                 <  2:535250/122‑1 (MQ=255)                          gCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTaa                                                                                                     >  1:87874/1‑150 (MQ=255)                               gatgatATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAActgct                                                                                                >  2:1372773/1‑150 (MQ=255)                                    tatTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGcc                                                                                           >  2:536313/1‑150 (MQ=255)                                    tatTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCAGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGcc                                                                                           >  1:801151/1‑150 (MQ=255)                                      ttttCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCgg                                                                                         >  2:1098518/1‑150 (MQ=255)                                        ttCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTg                                                                                       <  2:375648/150‑1 (MQ=255)                                         tCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGc                                                                                      <  1:616807/150‑1 (MQ=255)                                         tCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGc                                                                                      <  1:1372773/150‑1 (MQ=255)                                          cAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCg                                                                                     <  1:562418/150‑1 (MQ=255)                                            aaGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGtt                                                                                   <  2:384631/150‑1 (MQ=255)                                               gCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTg                                                                                <  2:1156288/150‑1 (MQ=255)                                                 ttcttTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGtt                                                                              <  2:990833/150‑1 (MQ=255)                                                     ttGATTATGACGCGCTGAATGCCTATTGAATGAAGATGACCCGCGATGCCGTATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGAAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATc                                                                          <  1:898366/150‑1 (MQ=255)                                                     ttGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATc                                                                          <  2:801151/150‑1 (MQ=255)                                                      tGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCa                                                                         >  2:1297072/1‑150 (MQ=255)                                                       gATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAg                                                                        <  1:412079/150‑1 (MQ=255)                                                                gcgcTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATg                                                               <  1:1122362/150‑1 (MQ=255)                                                                 cgcTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGc                                                              <  2:87874/150‑1 (MQ=255)                                                                         gCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATgcg                                                      <  2:1092068/150‑1 (MQ=255)                                                                          ccTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATgcgc                                                     >  1:1078881/1‑150 (MQ=255)                                                                          ccTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATgcgc                                                     >  1:894044/1‑150 (MQ=255)                                                                            tATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTg                                                   <  1:205034/150‑1 (MQ=255)                                                                             aTTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTgg                                                  <  2:198185/150‑1 (MQ=255)                                                                                         tGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTAc                                      >  1:88133/1‑150 (MQ=255)                                                                                                   tGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAg                                            <  2:164517/134‑1 (MQ=255)                                                                                                   tGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAg                                            >  1:164517/1‑134 (MQ=255)                                                                                                      cGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGAc                         >  1:232691/1‑150 (MQ=255)                                                                                                      cGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGAAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGAc                         <  1:1399740/150‑1 (MQ=255)                                                                                                       gAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGCCt                        >  2:1388294/1‑150 (MQ=255)                                                                                                              aTTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTccc                 <  1:1427141/150‑1 (MQ=255)                                                                                                              aTTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTccc                 >  2:1305300/1‑150 (MQ=255)                                                                                                              aTTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTccc                 >  2:884719/1‑150 (MQ=255)                                                                                                               tttAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTcccc                >  2:625366/1‑149 (MQ=255)                                                                                                                  aGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTa             >  1:854751/1‑150 (MQ=255)                                                                                                                            aTGGAAGCCAGCCTGATGGAGTCGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATc   >  1:884843/1‑150 (MQ=255)                                                                                                                             tGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCg  >  1:535104/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                     CTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCG  >  NC_000913/2623635‑2623908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑