Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,648,452	C→A	11.8%	G1279V (GGT→GTT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,648,452	0	C	A	11.8%	185.8 / 10.5	59	G1279V (GGT→GTT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base A (4/3);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCA  >  NC_000913/2648303‑2648596                                                                                                                                                      |                                                                                                                                                  cACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTAc                                                                                                                                                   >  2:686516/1‑150 (MQ=255)     cAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCg                                                                                                                                               >  1:1001988/1‑150 (MQ=255)      aGCGCACCCAGCGGAGCGTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCgg                                                                                                                                              <  1:642829/150‑1 (MQ=255)      aGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCgg                                                                                                                                              >  2:395862/1‑150 (MQ=255)            cccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACAGGATCGACATCATGCCCGGATCTTTTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATg                                                                                                                                        <  2:1247574/150‑1 (MQ=255)             ccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATgag                                                                                                                                      <  2:847482/150‑3 (MQ=255)                gCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGt                                                                                                                                   >  1:1336927/1‑150 (MQ=255)                 cGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTc                                                                                                                                  <  2:1176589/150‑1 (MQ=255)                 cGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTc                                                                                                                                  <  1:857992/150‑1 (MQ=255)                    aGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgc                                                                                                                               <  1:1095564/150‑2 (MQ=255)                       cTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCgg                                                                                                                            <  1:429569/150‑1 (MQ=255)                        ttttGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGc                                                                                                                           <  1:395862/150‑1 (MQ=255)                               gACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGt                                                                                                                    >  2:120321/1‑150 (MQ=255)                                        acacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGtt                                                                                                           >  1:867116/1‑150 (MQ=255)                                         cacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTc                                                                                                          >  1:1108459/1‑150 (MQ=255)                                           ccAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGc                                                                                                        <  2:180557/150‑1 (MQ=255)                                                 cagcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTGGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcgc                                                                                                  <  1:756345/150‑1 (MQ=255)                                                  agcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGcgcg                                                                                                 <  1:44749/150‑1 (MQ=255)                                                  agcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGcgcg                                                                                                 <  1:1178545/150‑1 (MQ=255)                                                   gcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCgg                                                                                                >  2:333680/1‑150 (MQ=255)                                                   gcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCgg                                                                                                >  2:304113/1‑150 (MQ=255)                                                       aaGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCa                                                                                            <  1:798032/150‑1 (MQ=255)                                                        aGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAg                                                                                           <  2:1336927/150‑1 (MQ=255)                                                               aCGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATcc                                                                                    <  2:478322/150‑1 (MQ=255)                                                                         tACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAg                                                                          <  1:313969/150‑1 (MQ=255)                                                                            tGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCg                                                                       >  2:1212599/1‑150 (MQ=255)                                                                             ggCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGt                                                                      >  2:886803/1‑150 (MQ=255)                                                                               cTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACACCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCa                                                                    >  2:1279284/1‑150 (MQ=255)                                                                                     gaTTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGt                                                              <  1:574591/150‑1 (MQ=255)                                                                                      aTTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTa                                                             <  1:686516/150‑1 (MQ=255)                                                                                            agcgTAACGGATAGACATAAAGCCAGGATCTTGTAAAAACCCCAGCAGACGCTAATAACCCCGAAAAAAG‑GCGTCTGTAGGCACGCTGTAACCCTGTTCGCCAGCGCGGACCAGGAAATACATCACGTAAGCCGTCAGCCAGTACTAtta                                                       >  2:609039/2‑149 (MQ=255)                                                                                                  cGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCAcc                                                 <  1:120321/150‑1 (MQ=255)                                                                                                    ggATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGt                                               >  2:1252962/1‑150 (MQ=255)                                                                                                      aTCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCATCAGCCAGTACTCTTCGTCACCGttt                                             >  1:892656/1‑150 (MQ=255)                                                                                                       tCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGtttt                                            <  2:1108459/150‑1 (MQ=255)                                                                                                        cGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGttttt                                           >  2:1280795/1‑150 (MQ=255)                                                                                                         gACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTa                                          >  1:804229/1‑150 (MQ=255)                                                                                                         gACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTa                                          >  1:465807/1‑150 (MQ=255)                                                                                                         gACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTa                                          >  2:524335/1‑150 (MQ=255)                                                                                                              catGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCca                                     >  2:270411/1‑150 (MQ=255)                                                                                                                tGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCcaca                                   <  1:38319/150‑1 (MQ=255)                                                                                                                  cccGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTTGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgc                                 >  1:341678/1‑150 (MQ=255)                                                                                                                        tCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAg                           <  1:524335/150‑1 (MQ=255)                                                                                                                        tCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAg                           <  2:319939/150‑1 (MQ=255)                                                                                                                         cTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAgc                          >  1:593166/1‑150 (MQ=255)                                                                                                                          ttGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAgcc                         >  1:599695/1‑150 (MQ=255)                                                                                                                              aaaTAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGccgccg                     >  1:1201297/1‑150 (MQ=255)                                                                                                                                  aGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTAt                 >  1:1268239/1‑150 (MQ=255)                                                                                                                                   gcgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATc                >  1:1290298/1‑150 (MQ=255)                                                                                                                                   gcgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATc                <  2:833017/150‑1 (MQ=255)                                                                                                                                    gccgacagACGC‑CATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCa               >  1:52092/6‑150 (MQ=38)                                                                                                                                       gacagACGC‑CATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGt            >  2:532841/3‑150 (MQ=255)                                                                                                                                       gacagACGC‑CATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACCTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGt            >  2:1387639/3‑150 (MQ=255)                                                                                                                                         cagACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTg          >  2:1293285/1‑150 (MQ=255)                                                                                                                                         cagACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTg          <  2:599695/150‑1 (MQ=255)                                                                                                                                          agACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCCTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTga         >  1:866815/1‑149 (MQ=255)                                                                                                                                           gACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCa        <  2:777585/150‑1 (MQ=255)                                                                                                                                            gacgccATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCa        >  2:1002858/6‑150 (MQ=255)                                                                                                                                                tCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgc   <  2:1268239/150‑1 (MQ=255)                                                                                                                                                 cATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgca  <  2:867116/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCA  >  NC_000913/2648303‑2648596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑