Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,050,713 A→T 7.1% intergenic (‑46/‑245) gcvT ← / → yqfI aminomethyltransferase/protein YqfI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,050,7130AT 7.1% 171.5 / 3.3 56intergenic (‑46/‑245)gcvT/yqfIaminomethyltransferase/protein YqfI
Reads supporting (aligned to +/- strand):  ref base A (36/16);  new base T (2/2);  total (38/18)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATCGC  >  NC_000913/3050570‑3050862
                                                                                                                                               |                                                                                                                                                     
tGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGTGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGCCAACTTTTTCATGGTGAAATTATc                                                                                                                                                 >  2:456605/1‑150 (MQ=255)
   gAACCGTAATGCAGCGACATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATcacc                                                                                                                                              <  2:940131/150‑1 (MQ=255)
        gTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGTTAATTTCACCATGaa                                                                                                                                         <  1:1159411/150‑1 (MQ=255)
        gTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGaa                                                                                                                                         >  1:1121320/1‑150 (MQ=255)
          aaTGCAGCGGCATCATCCAGCCGGGGAACTCCACCAGGCGAGCGCCGCAAAGCGGGTGGTGGTCGTACAAAGGAGTCTGTTGGGCCAGCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAAc                                                                                                                                       <  1:938911/150‑1 (MQ=255)
            tGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCGTGGTGATAATTTCACCATGaaaaag                                                                                                                                     <  1:1112466/150‑4 (MQ=255)
                       catcCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGc                                                                                                                          <  2:99884/150‑1 (MQ=255)
                                gTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCgg                                                                                                                 >  2:214332/1‑150 (MQ=255)
                                  ggAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTc                                                                                                               >  1:601876/1‑150 (MQ=255)
                                        ccaccaTGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAAc                                                                                                         >  1:1139475/1‑150 (MQ=255)
                                                cGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                                                        >  1:310318/1‑143 (MQ=255)
                                                cGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                                                        <  2:310318/143‑1 (MQ=255)
                                                  aGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTtc                                                                                               <  2:1206432/150‑1 (MQ=255)
                                                     gccgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTctct                                                                                            >  1:131719/1‑150 (MQ=255)
                                                      ccgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCtt                                                                                           >  2:239350/1‑150 (MQ=255)
                                                        gcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCtttt                                                                                         >  1:537830/1‑150 (MQ=255)
                                                          aaaGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGc                                                                                       <  1:1207103/150‑1 (MQ=255)
                                                                  tgttgtTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACAACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACtt                                                                               >  2:482076/1‑150 (MQ=255)
                                                                      gttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTacca                                                                           >  2:1228664/1‑150 (MQ=255)
                                                                      gttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTacca                                                                           >  1:747785/1‑150 (MQ=255)
                                                                               aaGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAg                                                                  >  2:268602/1‑150 (MQ=255)
                                                                                    gTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGt                                                             <  1:214332/150‑1 (MQ=255)
                                                                                    gTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGt                                                             >  2:301718/1‑150 (MQ=255)
                                                                                      cTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTa                                                           <  2:799070/150‑1 (MQ=255)
                                                                                        gttgtGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAAc                                                         <  1:423726/150‑1 (MQ=255)
                                                                                             gCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATaa                                                    >  1:888125/1‑150 (MQ=255)
                                                                                               cATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAgg                                                  <  2:1021664/150‑1 (MQ=255)
                                                                                                     gTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTGTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAAt                                            >  2:617650/1‑150 (MQ=255)
                                                                                                       ccTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTg                                          >  2:852954/1‑150 (MQ=255)
                                                                                                        cTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGa                                         >  1:60210/1‑150 (MQ=255)
                                                                                                         tCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGat                                        >  2:972544/1‑150 (MQ=255)
                                                                                                          cATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatc                                       >  1:1313177/1‑150 (MQ=255)
                                                                                                           aTTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatca                                      <  2:888125/150‑1 (MQ=255)
                                                                                                             tGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatgatc                                    >  1:619801/1‑150 (MQ=255)
                                                                                                               aaTAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATcac                                  <  1:268602/150‑1 (MQ=255)
                                                                                                               aaTAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATcac                                  >  2:114793/1‑150 (MQ=255)
                                                                                                                 tAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTtctc                                                                                             >  1:1187523/1‑89 (MQ=255)
                                                                                                                 tAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTtctc                                                                                             <  2:1187523/89‑1 (MQ=255)
                                                                                                                    ggggggCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCGACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTAGCACCGAAACTGACTGTTAACGATAAGGTAATTTTGATCATCACATTAg                             <  2:1315906/148‑1 (MQ=255)
                                                                                                                      ggggCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCt                           <  1:239350/150‑1 (MQ=255)
                                                                                                                          cTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATg                       >  1:36349/1‑150 (MQ=255)
                                                                                                                          cTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATg                       >  1:1224524/1‑150 (MQ=255)
                                                                                                                            gACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGt                     >  1:599861/1‑150 (MQ=255)
                                                                                                                              cAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTa                   >  2:571991/1‑150 (MQ=255)
                                                                                                                              cAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCATATGGTTa                   >  1:1257805/1‑150 (MQ=255)
                                                                                                                               aaCTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaa                  >  1:367351/1‑150 (MQ=255)
                                                                                                                                aCTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGt                                                 <  2:358126/118‑1 (MQ=255)
                                                                                                                                aCTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGt                                                 >  1:358126/1‑118 (MQ=255)
                                                                                                                                aCTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaa                 >  2:1054698/1‑150 (MQ=255)
                                                                                                                                 cTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTtaaa                >  2:1063839/1‑150 (MQ=255)
                                                                                                                                  tttttCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaaaa               >  1:478034/1‑150 (MQ=255)
                                                                                                                                   gtttCGTGGTGATAATTTCACCATGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTaaaaaa              >  1:694745/2‑150 (MQ=255)
                                                                                                                                     ttCGTGGTGATAATTTCACCATGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATg            >  1:699321/1‑150 (MQ=255)
                                                                                                                                     ttCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATg            >  1:25418/1‑150 (MQ=255)
                                                                                                                                          ggTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCaaaa       >  1:863666/1‑150 (MQ=255)
                                                                                                                                           gTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCaaaaa      >  1:1070195/1‑150 (MQ=255)
                                                                                                                                               aaTTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATcgc  <  2:848297/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
TGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATCATCACATTAGCTTATGGTTAAAAAATGCAAAAATCGC  >  NC_000913/3050570‑3050862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑