| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,524,463 | Δ1 bp | 7.8% | intergenic (+95/+18) | yncE → / ← ansP | PQQ‑like domain‑containing protein YncE/L‑asparagine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,524,463 | 0 | A | . | 7.8% | 293.7 / 16.6 | 90 | intergenic (+95/+18) | yncE/ansP | PQQ‑like domain‑containing protein YncE/L‑asparagine transporter |
| Reads supporting (aligned to +/- strand): ref base A (35/48); new base . (5/2); total (40/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
CACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATAC > NC_000913/1524314‑1524608 | cACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTa > 2:361914/1‑150 (MQ=255)cACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTa > 1:177203/1‑150 (MQ=255) tAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGGGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGcc < 1:443922/150‑1 (MQ=255) aaaCAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCg > 1:1350861/1‑150 (MQ=255) aaaCAGCAGGAAGCTACCAAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCg > 2:1221573/1‑150 (MQ=255) agcagGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCa < 1:440933/150‑1 (MQ=255) agcagGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCa > 1:856763/1‑150 (MQ=255) ggAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAAttt > 2:643933/1‑150 (MQ=255) aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCAAattcag < 2:1267520/150‑4 (MQ=255) aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGc < 1:244345/150‑1 (MQ=255) aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGGGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGc < 1:1306968/150‑1 (MQ=255) gCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCa < 2:965864/150‑1 (MQ=255) tACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAAt < 1:769861/150‑1 (MQ=255) ccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCg > 2:1466772/1‑150 (MQ=255) ccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCg > 2:321259/1‑150 (MQ=255) agACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGcc > 1:355518/1‑150 (MQ=255) cGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGtt > 2:1373033/1‑150 (MQ=255) aTGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc < 2:861194/150‑1 (MQ=255) tgtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCa > 2:418601/1‑150 (MQ=255) gtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAg < 1:386347/150‑1 (MQ=255) gATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc < 1:1434249/150‑1 (MQ=255) gATTCGTATTGCGCTGTAATAGATAAGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc > 2:1368497/1‑150 (MQ=255) aTTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCt > 2:1268404/1‑150 (MQ=255) gcgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCCGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACaa > 1:904532/1‑150 (MQ=255) gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc < 1:1373033/150‑1 (MQ=255) gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCGGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc < 2:139172/150‑1 (MQ=255) tAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCt < 1:662994/150‑1 (MQ=255) gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt > 2:1300115/1‑150 (MQ=255) tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc > 2:792864/1‑127 (MQ=255) tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc < 1:792864/127‑1 (MQ=255) tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAATAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttc < 1:269777/150‑1 (MQ=255) atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 1:298265/150‑1 (MQ=255) atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 1:303878/150‑1 (MQ=255) atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCACCGTTTCAGGCTTAAACACAATTTCCTGTTTttct > 1:1214728/1‑150 (MQ=255) tGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt < 2:1021398/150‑1 (MQ=255) ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc < 1:1239578/150‑1 (MQ=255) gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa < 2:1145812/150‑1 (MQ=255) cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa < 1:355588/149‑1 (MQ=255) cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa > 2:355588/1‑149 (MQ=255) cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGCCTTAAACACAATTTCCTGTTTTTCTTCAt > 2:504280/1‑150 (MQ=255) ggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTc < 1:1300115/150‑1 (MQ=255) gTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTcc > 1:172871/1‑150 (MQ=255) tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt < 2:1462333/150‑1 (MQ=255) tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt > 1:1411557/1‑150 (MQ=255) aaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcga > 1:1147335/1‑150 (MQ=255) cGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacga < 1:418601/150‑1 (MQ=255) ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAAtt > 2:243774/1‑92 (MQ=255) ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAAtt < 1:243774/92‑1 (MQ=255) tGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg < 1:1269818/150‑1 (MQ=255) gaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcg > 2:1002005/1‑150 (MQ=255) gaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcg > 2:155276/1‑150 (MQ=255) cTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCa > 2:610063/1‑150 (MQ=255) cTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCa > 1:1096957/1‑150 (MQ=255) cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgtg < 2:1350861/150‑1 (MQ=255) cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCATCGACGACTGGCGCAGTGCtgtg < 1:1392533/150‑1 (MQ=255) tATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAAttt > 1:610417/1‑150 (MQ=255) aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTc < 2:1507216/150‑1 (MQ=255) aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAACTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTTCTGTGAATTTc < 1:966842/150‑1 (MQ=255) tCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa > 2:1211580/1‑150 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCTGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg < 1:466641/150‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg < 1:1152082/150‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg < 2:502773/150‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg > 1:343742/1‑150 (MQ=255) cGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTTGCTCAGTGCTGTGAATTTCAGCAAcg < 1:1040464/150‑1 (MQ=255) tATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcgcg > 2:1189712/1‑150 (MQ=255) tCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGtt > 1:1482152/1‑150 (MQ=255) gCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTgcg < 2:423124/150‑1 (MQ=255) cACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTgcgc < 2:29104/150‑1 (MQ=255) cTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcac > 2:838685/1‑150 (MQ=255) tCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACAcc < 1:361914/150‑1 (MQ=255) tCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCaaa > 1:30187/1‑150 (MQ=255) cAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAc < 1:1466772/150‑1 (MQ=255) aGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc < 1:1189712/150‑1 (MQ=255) aaTCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGc < 1:916430/150‑1 (MQ=255) aTCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc < 2:832315/150‑1 (MQ=255) aTCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc < 1:432703/150‑1 (MQ=255) caaaattGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCtat > 2:186198/6‑150 (MQ=255) tttGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAcc < 1:1368497/150‑1 (MQ=255) tttGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAcc < 1:1268404/150‑1 (MQ=255) tttGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAcc < 1:838685/150‑1 (MQ=255) tGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAg > 2:1271330/1‑150 (MQ=255) tGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCCGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAg > 1:1224875/1‑150 (MQ=255) gCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGc > 1:1475144/1‑150 (MQ=255) gCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGc > 1:145994/1‑150 (MQ=255) cccGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCTGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCa > 2:705334/1‑150 (MQ=255) cccGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTCGCGCAGTGCTGTGAATTTAAGCAACGAGTTTGCGCACAACAAACCAGCCTATAACCAGCa > 1:1420660/1‑150 (MQ=255) ccGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTTAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaa > 2:411192/1‑150 (MQ=255) cGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCTCACACCAAACCAGCCTATAACCAGCaaa < 1:155276/150‑1 (MQ=255) cGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaaa < 1:1221573/150‑1 (MQ=255) cGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaaa < 1:990710/150‑1 (MQ=255) cGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaaa < 1:1002005/150‑1 (MQ=255) aCGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATAc < 2:355518/150‑1 (MQ=255) | CACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATAC > NC_000913/1524314‑1524608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |