Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,491 G→C 7.3% P14R (CCG→CGG)  iscS ← cysteine desulfurase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,4910GC 7.3% 269.7 / 10.1 82P14R (CCG→CGG) iscScysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base G (34/42);  new base C (3/3);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

CGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTT  >  NC_000913/2661345‑2661639
                                                                                                                                                   |                                                                                                                                                    
cGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCg                                                                                                                                                   >  1:453459/1‑150 (MQ=255)
 gACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGt                                                                                                                                                  <  2:760698/150‑1 (MQ=255)
 gACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGt                                                                                                                                                  >  1:669067/1‑150 (MQ=255)
 gACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGt                                                                                                                                                  >  1:275799/1‑150 (MQ=255)
   ccAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTgg                                                                                                                                                <  1:600765/150‑1 (MQ=255)
      gATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTg                                                                                                                                             >  1:696463/1‑150 (MQ=255)
      gATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCCGGTGGACCCGCGTgttgc                                                                                                                                             <  2:677481/150‑5 (MQ=255)
          ggCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTGACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGa                                                                                                                                         <  1:319348/150‑1 (MQ=255)
           gCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAATCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGag                                                                                                                                        <  1:1309719/150‑1 (MQ=255)
           gCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGag                                                                                                                                        <  1:956950/150‑1 (MQ=255)
            cAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagt                                                                                                                                       >  2:673481/1‑150 (MQ=255)
            cAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagt                                                                                                                                       <  1:911169/150‑1 (MQ=255)
             aaTCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaa                                                                                                                                      <  1:599118/150‑3 (MQ=38)
              aTCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagtag                                                                                                                                     >  1:1151279/1‑150 (MQ=255)
               tCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGagtagt                                                                                                                                    >  2:1339094/1‑150 (MQ=255)
                 tGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCg                                                                                                                                  >  2:1464315/1‑150 (MQ=255)
                  gATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCga                                                                                                                                 <  1:1411026/150‑1 (MQ=255)
                   aTTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCgag                                                                                                                                <  1:537920/150‑1 (MQ=255)
                     tACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGAt                                                                                                                              <  2:1057464/150‑1 (MQ=255)
                     tACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGAt                                                                                                                              <  1:596813/150‑1 (MQ=255)
                          gCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATc                                                                                                                         <  1:923318/150‑1 (MQ=255)
                                tCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAt                                                                                                                   >  2:1439297/1‑150 (MQ=255)
                                tCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAt                                                                                                                   >  1:1177634/1‑150 (MQ=255)
                                 cTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAtt                                                                                                                  >  1:409785/1‑150 (MQ=255)
                                  tACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                                                                                                                 >  2:1115279/1‑150 (MQ=255)
                                  tACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAAttt                                                                                                                 >  1:1418910/1‑150 (MQ=255)
                                   aCCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTc                                                                                                                >  1:881424/1‑150 (MQ=255)
                                     cGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGGACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCAt                                                                                                              <  2:683100/150‑1 (MQ=255)
                                     cGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCAt                                                                                                              <  1:115623/150‑1 (MQ=255)
                                        ttcttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGc                                                                                                           <  2:1177634/150‑1 (MQ=255)
                                           ttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctct                                                                                                        <  2:1284676/150‑1 (MQ=255)
                                           ttcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGctct                                                                                                        <  2:441368/150‑1 (MQ=255)
                                            tcAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTTCTCta                                                                                                       <  2:483462/150‑1 (MQ=255)
                                             cAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCtat                                                                                                      >  2:252066/1‑150 (MQ=255)
                                              aGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCtata                                                                                                     >  2:728594/1‑150 (MQ=255)
                                                 cTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAAc                                                                                                  <  2:409785/150‑1 (MQ=255)
                                                   gCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTc                                                                                                <  2:94538/150‑1 (MQ=255)
                                                     cAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCg                                                                                              >  1:54883/1‑150 (MQ=255)
                                                        ccGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTAc                                                                                           <  2:990726/150‑1 (MQ=255)
                                                        ccGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTAc                                                                                           <  1:902804/150‑1 (MQ=255)
                                                           aaaCGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATc                                                                                        <  2:902421/150‑1 (MQ=255)
                                                           aaaCGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATc                                                                                        <  2:669067/150‑1 (MQ=255)
                                                           aaaCGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATc                                                                                        <  1:537484/150‑1 (MQ=255)
                                                             aCGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCAc                                                                                      >  2:1372442/1‑150 (MQ=255)
                                                                 tGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCaa                                                                                  >  2:1148118/1‑150 (MQ=255)
                                                                       cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAg                                                                            <  1:1163607/150‑1 (MQ=255)
                                                                       cGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAg                                                                            <  2:38887/150‑1 (MQ=255)
                                                                        gggAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAgg                                                                           <  1:587402/150‑1 (MQ=255)
                                                                         ggAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAAAGCAAGGa                                                                          >  2:770260/1‑150 (MQ=255)
                                                                            ggCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATc                                                                       >  1:1008691/1‑150 (MQ=255)
                                                                              ccGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAg                                                                     >  1:373951/1‑150 (MQ=255)
                                                                              ccGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGCAGTAGTCCAGATAAATCGGTAATTTCATTGCTCTATAAACTCAGTACATCACTCAATGCAAGGAATCaa                                                                     >  1:579212/1‑149 (MQ=255)
                                                                              ccGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaa                                                                                                                                      <  1:289183/85‑3 (MQ=17)
                                                                              ccGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCCGCAACCACGCCGGTGGACCCGCGT‑GTTGCCGagaa                                                                                                                                      >  2:289183/1‑83 (MQ=17)
                                                                               cGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAgg                                                                    <  1:595462/150‑1 (MQ=255)
                                                                                           ggTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGa                                                        <  1:1339094/150‑1 (MQ=255)
                                                                                              tCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGt                                                     >  2:623719/1‑150 (MQ=255)
                                                                                                 gTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACg                                                  <  2:300542/150‑1 (MQ=255)
                                                                                                             aaaCTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACg                                      <  2:1008691/150‑1 (MQ=255)
                                                                                                                  gCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTa                                 >  1:523861/1‑150 (MQ=255)
                                                                                                                   catcatTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTAt                                >  2:682168/1‑150 (MQ=255)
                                                                                                                      catTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTa                             <  1:728594/150‑1 (MQ=255)
                                                                                                                      catTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTa                             <  2:696463/150‑1 (MQ=255)
                                                                                                                      catTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTa                             <  2:603565/150‑1 (MQ=255)
                                                                                                                        ttttCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGc                           <  1:770260/150‑1 (MQ=255)
                                                                                                                          ttCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCAc                         >  1:364761/1‑150 (MQ=255)
                                                                                                                            ctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGc                           >  2:1470621/1‑146 (MQ=255)
                                                                                                                            ctcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGc                           <  1:1470621/146‑1 (MQ=255)
                                                                                                                             tcGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTcc                      >  2:106890/1‑150 (MQ=255)
                                                                                                                              ccgcaaccacGCCGGTGGACCCGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTcc                      >  1:779834/8‑150 (MQ=255)
                                                                                                                               ggCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGCCCGTGTTTACGGAGTATTTAGCACTCCgg                    >  1:1212851/1‑150 (MQ=255)
                                                                                                                                 cAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGcc                  <  1:715014/150‑1 (MQ=255)
                                                                                                                                   accacGCCGGTGGACCCGCGTGGTTGCGGAGTAGTCGAGATAACTCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCt                 >  1:867329/3‑150 (MQ=255)
                                                                                                                                        gcgGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTc            <  1:1464315/150‑1 (MQ=255)
                                                                                                                                         cgGGTCCACCGGCGTCCTTGCGCAGTAGTCGAGATAAATCGGTAATATAATTGCTCAATAAACACCCTACATAACACAAACAAAGCAATCAGGCTAAATGCTGCATCAACGCCCGAGTTTAATGAGTATTTAGCACTCCGGCCAGATTCt           >  2:227940/1‑150 (MQ=255)
                                                                                                                                           ggTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGa         >  2:528308/1‑150 (MQ=255)
                                                                                                                                           ggTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGa         <  1:628613/150‑1 (MQ=255)
                                                                                                                                            gTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGaa        <  1:20427/150‑1 (MQ=255)
                                                                                                                                             tCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGGTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAAt       <  2:275799/150‑1 (MQ=255)
                                                                                                                                             tCCACCGGCGTGGTTCCGGAGTAGTCGACATAAAACGGTAATTTAATAGCACTATAAACTCCGTACATCACTCAAGGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGACTATTTAGCAAACCGGCCTGATTCTGAAt       >  2:1219536/1‑150 (MQ=255)
                                                                                                                                              ccACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAAtt      >  2:543250/1‑150 (MQ=255)
                                                                                                                                                  cGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCttt  <  2:54883/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGACCAGATCGGCAATCTGATTACGGGCGATATCTACCGCTTCTTCAGCCTGCCAGCCGAAACGGTGAGAACGGGAGGCCGGGTTACCAAAGGTTCCGTCCATCGTCATAAACTGCATCATTTTCTCGGCAA‑CACGCGGGTCCACCGGCGTGGTTGCGGAGTAGTCGAGATAAATCGGTAATTTCATTGCTCTATAAACTCCGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTT  >  NC_000913/2661345‑2661639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑