BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	329,503	A→G	9.5%	N14S (AAT→AGT)	betT →	choline:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	329,503	0	A	G	9.5%	305.4 / 16.5	105	N14S (AAT→AGT)	betT	choline:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (52/43); new base G (4/6); total (56/49)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGC  >  NC_000913/329356‑329652

        aTCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGgtggtg                                                                                                                                             >  2:826505/1‑150 (MQ=255)

         tCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGgtgt                                                                                                                                            >  1:1301801/1‑150 (MQ=255)

           tATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGttt                                                                                                                                          >  1:1478397/1‑150 (MQ=255)

            aTTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGtttt                                                                                                                                         >  2:974933/1‑150 (MQ=255)

            aTTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGtttt                                                                                                                                         >  2:1135043/1‑150 (MQ=255)

              tAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTac                                                                                                                                       >  2:621536/1‑150 (MQ=255)

              tAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTac                                                                                                                                       >  2:38627/1‑150 (MQ=255)

                aaGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTacac                                                                                                                                     >  2:571226/1‑150 (MQ=255)

                aaGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTacac                                                                                                                                     <  1:782228/150‑1 (MQ=255)

                 aGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGTCAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACAcc                                                                                                                                    <  2:781287/150‑1 (MQ=255)

                 aGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACAcc                                                                                                                                    >  2:98664/1‑150 (MQ=255)

                 aGCGGTTATTGATTGGACGTTCAATATAAAATGTGCCTTAATTGTTACGAATTTGATTTAAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACAcc                                                                                                                                    <  1:801534/150‑1 (MQ=255)

                       tATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTccgcc                                                                                                                              >  2:938031/1‑150 (MQ=255)

                          tgattgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGa                                                                                                                           >  1:290031/1‑150 (MQ=255)

                               ggACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAt                                                                                                                      >  2:177002/1‑150 (MQ=255)

                                gACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAtt                                                                                                                     <  1:1514871/150‑1 (MQ=255)

                                 aCGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTccgccg                                                                                                                             >  2:1467441/1‑141 (MQ=255)

                                 aCGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAttt                                                                                                                    <  1:1084310/150‑1 (MQ=255)

                                   gTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttg                                                                                                                  >  1:928779/1‑150 (MQ=255)

                                   gTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttg                                                                                                                  >  1:569108/1‑150 (MQ=255)

                                    ttCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgt                                                                                                                 <  2:928779/150‑1 (MQ=255)

                                    ttCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgt                                                                                                                 <  1:1363469/150‑1 (MQ=255)

                                    ttCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgt                                                                                                                 <  1:1530886/150‑1 (MQ=255)

                                     tCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgtt                                                                                                                <  1:422235/150‑1 (MQ=255)

                                     tCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgtt                                                                                                                <  1:331883/150‑1 (MQ=255)

                                      cAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGTGGCT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgttg                                                                                                               <  1:1488680/150‑1 (MQ=255)

                                      cAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGTCAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgttg                                                                                                               <  1:1000697/150‑1 (MQ=255)

                                      cAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgttg                                                                                                               <  2:462239/150‑1 (MQ=255)

                                       aaTATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTtgttgt                                                                                                              >  2:381880/1‑150 (MQ=255)

                                        atatAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTgttgtt                                                                                                             >  1:306325/1‑150 (MQ=255)

                                         tataAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGttt                                                                                                            <  1:119049/150‑1 (MQ=255)

                                         tataAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGttt                                                                                                            <  2:102096/150‑1 (MQ=255)

                                          ataAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGtttt                                                                                                           >  2:284746/1‑150 (MQ=255)

                                           taAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAAACCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTc                                                                                                          >  2:948913/1‑150 (MQ=255)

                                           taAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTc                                                                                                          <  1:762182/150‑1 (MQ=18)

                                             aaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTccc                                                                                                        <  1:1353947/150‑1 (MQ=255)

                                             aaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTccc                                                                                                        <  1:381880/150‑1 (MQ=255)

                                             aaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTccc                                                                                                        <  1:786173/150‑1 (MQ=18)

                                              aaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGTCCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGTTTGATTTTGTCCTTTTCCCt                                                                                                       <  2:1267/150‑1 (MQ=18)

                                              aaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCt                                                                                                       <  1:837311/150‑1 (MQ=18)

                                                 gtgtCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGAc                                                                                                    <  1:735760/150‑1 (MQ=255)

                                                       tAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGAt                                                                                              >  2:356209/1‑150 (MQ=255)

                                                         aTTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATcc                                                                                            <  1:826505/150‑1 (MQ=255)

                                                          ttGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCt                                                                                           >  1:282093/1‑150 (MQ=255)

                                                            gTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGt                                                                                         >  1:575178/1‑150 (MQ=255)

                                                                 taaTTTTTTTTAAAATAGAAAAAATAACA‑TGGGGCTAACTGTATGCCATACCTTTCACACAGCCGGTAAACGTACAAATTAAAT‑CGGTGGGTGTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTcgcg                                                                                   <  1:638187/149‑1 (MQ=255)

                                                                 gAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttg                                                                                                                  >  1:195903/1‑120 (MQ=255)

                                                                 gAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttg                                                                                                                  <  2:195903/120‑1 (MQ=255)

                                                                 gAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTcgc                                                                                    >  2:125289/1‑150 (MQ=255)

                                                                   aTTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGa                                                                                  >  2:1149250/1‑150 (MQ=255)

                                                                   aTTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATACTGTTTCGCGa                                                                                  >  2:1361508/1‑150 (MQ=255)

                                                                    tttGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGAc                                                                                 >  1:1532710/1‑150 (MQ=255)

                                                                        aTTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCTGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTtct                                                                             >  1:362691/1‑150 (MQ=255)

                                                                             aaaTAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGcc                                                                        >  2:1096831/1‑150 (MQ=255)

                                                                                tAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCtg                                                                     <  2:1395458/150‑1 (MQ=255)

                                                                                  gTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCtgtg                                                                   <  1:188421/150‑1 (MQ=255)

                                                                                       aaTAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTg                                                              <  1:766373/150‑1 (MQ=255)

                                                                                       aaTAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTg                                                              >  1:1083741/1‑150 (MQ=255)

                                                                                        aTAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTgg                                                             <  2:1083741/150‑1 (MQ=255)

                                                                                                ggggAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGc                                                     >  1:1381460/1‑150 (MQ=255)

                                                                                                 gggAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCt                                                    >  1:16407/1‑150 (MQ=255)

                                                                                                        cTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTg                                              <  2:290309/150‑1 (MQ=255)

                                                                                                        cTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTg                                              <  2:700083/150‑1 (MQ=255)

                                                                                                          ggATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTggg                                            >  1:1354692/1‑150 (MQ=255)

                                                                                                           gATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGt                                           <  1:540747/150‑1 (MQ=255)

                                                                                                              gACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTc                                        <  2:354006/150‑1 (MQ=255)

                                                                                                               aCAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCt                                       <  2:306325/150‑1 (MQ=255)

                                                                                                                  gACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTaaa                                    >  2:1002096/1‑150 (MQ=255)

                                                                                                                      tttCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCt                                >  1:1017170/1‑150 (MQ=255)

                                                                                                                      tttCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCt                                >  1:1032506/1‑150 (MQ=255)

                                                                                                                        tCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTc                              <  2:1355093/150‑1 (MQ=255)

                                                                                                                        tCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTc                              <  2:980474/150‑1 (MQ=255)

                                                                                                                           cacaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTAGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGt                           >  2:567389/1‑150 (MQ=255)

                                                                                                                            ccagcagGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACCCCGCCGCCGGACTGATTTTGTTGTTTTCCCTGACCACGCTCCTGTGTCGCGCCTTCTCGGCCCGGTGGATTGGCCGCACGCTGGACTGGGGGTCTAAAACCTTCgggt                          >  1:1401809/2‑148 (MQ=255)

                                                                                                                            acaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGtt                          <  1:621536/150‑1 (MQ=255)

                                                                                                                            acaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGtt                          <  1:356209/150‑1 (MQ=255)

                                                                                                                             tgtcagGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCAGCGGATTGATTTTGTCCTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTg                         >  1:975569/4‑150 (MQ=17)

                                                                                                                                cagGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTa                      >  1:36213/1‑150 (MQ=18)

                                                                                                                                 agGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTAc                     >  1:693161/1‑150 (MQ=255)

                                                                                                                                  gggAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACt                    <  2:501759/150‑1 (MQ=255)

                                                                                                                                  gggAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACt                    >  2:791036/1‑150 (MQ=255)

                                                                                                                                   ggAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTa                   >  1:924570/1‑150 (MQ=255)

                                                                                                                                    gAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTAt                  >  1:1273645/1‑150 (MQ=255)

                                                                                                                                     aaaaGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATc                 <  1:1146824/150‑1 (MQ=255)

                                                                                                                                       aaGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctg               >  1:645512/1‑150 (MQ=255)

                                                                                                                                         ggACAAAATCAATCCCGTGGTGTTTTACACATCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgat             >  2:849853/1‑148 (MQ=255)

                                                                                                                                          gACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgctg            >  1:281965/1‑150 (MQ=255)

                                                                                                                                          aaCAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgctg            >  2:52913/2‑150 (MQ=25)

                                                                                                                                           aCAAAATCAGTCCGGCGGAGGTGTAAAACACCAGCGGATTGATTTTGTCCTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTgg           <  2:975569/150‑1 (MQ=21)

                                                                                                                                            cAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggc          >  2:1379398/1‑150 (MQ=255)

                                                                                                                                            cAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggc          >  2:1335997/1‑150 (MQ=255)

                                                                                                                                              aaaTCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcgg        >  2:1210063/1‑150 (MQ=255)

                                                                                                                                               aatcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcggc       >  2:418060/1‑150 (MQ=255)

                                                                                                                                               aatcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcggc       >  1:695484/1‑150 (MQ=255)

                                                                                                                                                atcaatcCGGTTGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCa      <  2:819941/150‑1 (MQ=255)

                                                                                                                                                atcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCa      >  1:160064/1‑150 (MQ=255)

                                                                                                                                                atcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGATTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGTCa      <  2:825197/150‑1 (MQ=255)

                                                                                                                                                 tcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCaa     >  1:1331653/1‑150 (MQ=255)

                                                                                                                                                   aatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACg   >  2:172526/1‑150 (MQ=255)

                                                                                                                                                    atcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCATGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTCGCGGCAACGc  >  2:295128/1‑150 (MQ=255)

CGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGAT‑ACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGC  >  NC_000913/329356‑329652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`