Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,776,135 A→C 6.7% N73H (AAT→CAT)  yfjX → CP4‑57 prophage; putative antirestriction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,776,1350AC 6.7% 275.8 / 10.8 90N73H (AAT→CAT) yfjXCP4‑57 prophage; putative antirestriction protein
Reads supporting (aligned to +/- strand):  ref base A (57/27);  new base C (3/3);  total (60/30)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

AGGTACCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCA  >  NC_000913/2775986‑2776282
                                                                                                                                                     |                                                                                                                                                   
aGGTACCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCa                                                                                                                                                     <  2:944543/150‑1 (MQ=255)
    aCCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATgg                                                                                                                                                 >  2:22164/1‑150 (MQ=255)
       cGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCgg                                                                                                                                              >  1:210650/1‑150 (MQ=255)
             aCAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCCGCCATTGCTGAGCGtg                                                                                                                                        <  2:233144/150‑2 (MQ=255)
                gCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTAt                                                                                                                                     >  1:606972/1‑150 (MQ=255)
                  atatCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTTATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCCGCCATTGCTGAGCGTGTaaaa                                                                                                                                   <  2:1458573/150‑4 (MQ=37)
                   tatCCGCTTCTGTCCGCAGCACTTTGGCACCATCCCGCAGTGTATAACGCTGTAAACGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTTGTCCTTTTACACGCTCCGCCATTGCTGAGCGTGTaaaag                                                                                                                                  <  2:1001048/150‑5 (MQ=37)
                   tatCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGc                                                                                                                                  >  2:766187/1‑150 (MQ=255)
                    atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGCGCGTTTATGGcg                                                                                                                                 >  2:1428115/1‑149 (MQ=255)
                    atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCt                                                                                                                                 >  1:713584/1‑150 (MQ=255)
                    atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCt                                                                                                                                 >  2:760076/1‑150 (MQ=255)
                      ccGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTcc                                                                                                                               <  2:568060/150‑1 (MQ=255)
                       cGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCt                                                                                                                              <  2:1467398/150‑1 (MQ=255)
                           tCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATg                                                                                                                          >  2:1331657/1‑150 (MQ=255)
                            cTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGc                                                                                                                         >  2:1111244/1‑150 (MQ=255)
                            cTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGc                                                                                                                         <  1:1002521/150‑1 (MQ=255)
                                   cagcaCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGt                                                                                                                  <  2:919464/150‑1 (MQ=255)
                                   cagcaCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGt                                                                                                                  <  2:213589/150‑1 (MQ=255)
                                      caCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGAc                                                                                                               <  1:1329444/150‑1 (MQ=255)
                                      caCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGAc                                                                                                               >  1:39657/1‑150 (MQ=255)
                                             gCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAAt                                                                                                        >  1:1431381/1‑150 (MQ=255)
                                              cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCg                                                                                                                                          >  2:559417/1‑115 (MQ=255)
                                              cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCg                                                                                                                                          <  1:559417/115‑1 (MQ=255)
                                              cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATg                                                                                                       >  1:475449/1‑150 (MQ=255)
                                              cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATg                                                                                                       <  2:683174/150‑1 (MQ=255)
                                                ccATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGc                                                                                                     >  1:1156472/1‑150 (MQ=255)
                                                ccATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGc                                                                                                     <  2:713584/150‑1 (MQ=255)
                                                   tCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCAtt                                                                                                  >  1:788834/1‑150 (MQ=255)
                                                      cGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGc                                                                                               <  1:1111244/150‑1 (MQ=255)
                                                       gCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCt                                                                                              >  2:981504/1‑150 (MQ=255)
                                                        cAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTc                                                                                             <  1:283633/150‑1 (MQ=255)
                                                         aGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCa                                                                                            >  1:508457/1‑150 (MQ=255)
                                                           tGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGGGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAAc                                                                                          >  1:1492971/1‑150 (MQ=255)
                                                            ggATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACg                                                                                         >  2:16045/1‑150 (MQ=255)
                                                            ggATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCGCAAcc                                                                                         >  2:683021/1‑149 (MQ=255)
                                                              aTAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGc                                                                                       >  1:481189/1‑150 (MQ=255)
                                                               tAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGAc                                                                                                                     <  1:1082825/119‑1 (MQ=255)
                                                               tAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGAc                                                                                                                     >  2:1082825/1‑119 (MQ=255)
                                                               tAACGCTCGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGCTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCa                                                                                      >  2:43818/1‑150 (MQ=255)
                                                                 aCGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATg                                                                                    >  2:246951/1‑150 (MQ=255)
                                                                    cTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAAt                                                                                 >  1:1453546/1‑150 (MQ=255)
                                                                         aCCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCaa                                                                            >  1:566782/1‑150 (MQ=255)
                                                                         aCCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCaa                                                                            >  1:911967/1‑150 (MQ=255)
                                                                           cgcgCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATg                                                                          >  1:900685/1‑150 (MQ=255)
                                                                           cgcgCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATg                                                                          >  2:1294687/1‑150 (MQ=255)
                                                                                 ttttCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCgg                                                                    >  2:582462/1‑150 (MQ=255)
                                                                                 ttttCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCgg                                                                    >  2:1374133/1‑150 (MQ=255)
                                                                                         tggatggaCCGCTTCTGTGATGAGTACTTCGGGGGTATCTGGTCCTTTTACACCCTCAGCAATGGCGGAGCGTTTATCGCTCCTGATGCTGACGGTGACGATAAATGGCATTTCCTCAACGGCATGAATGTCAATCGTGACGAAATAAgc                                                            >  2:1156381/1‑150 (MQ=37)
                                                                                          ggatggaCCGCTTCTGGGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAgcg                                                           <  1:747292/150‑1 (MQ=255)
                                                                                           gatggaCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAgcgc                                                          >  1:203495/1‑150 (MQ=255)
                                                                                             tggaCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCgg                                                        >  1:754289/1‑150 (MQ=255)
                                                                                                aCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAg                                                     >  2:902711/1‑150 (MQ=255)
                                                                                                   gCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCg                                                  >  2:571756/1‑150 (MQ=255)
                                                                                                    cTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGc                                                 <  2:754289/150‑1 (MQ=255)
                                                                                                     ttCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCa                                                <  2:1302466/150‑1 (MQ=255)
                                                                                                      tCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAg                                               >  1:724232/1‑150 (MQ=255)
                                                                                                         gtgATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTa                                            <  2:1431381/150‑1 (MQ=255)
                                                                                                         gtgATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTa                                            >  1:1114685/1‑150 (MQ=255)
                                                                                                          tgatgaGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTAt                                           <  1:766187/150‑1 (MQ=255)
                                                                                                                gTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGt                                     >  2:742543/1‑150 (MQ=255)
                                                                                                                   cTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTg                                  >  2:56166/1‑150 (MQ=255)
                                                                                                                    tGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGc                                 >  1:712627/1‑150 (MQ=255)
                                                                                                                     gCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGcc                                <  2:1453546/150‑1 (MQ=255)
                                                                                                                          ggTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                           <  1:16045/150‑1 (MQ=255)
                                                                                                                          ggTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                           <  2:210650/150‑1 (MQ=255)
                                                                                                                            tATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGa                         >  1:508930/1‑150 (MQ=255)
                                                                                                                              tCTGGTCCTTTTACACGCGCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAat                       <  2:1219877/150‑1 (MQ=255)
                                                                                                                               cTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                      <  2:788834/150‑1 (MQ=255)
                                                                                                                               cTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                      >  2:1408473/1‑150 (MQ=255)
                                                                                                                                tGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAatat                     >  2:714439/1‑150 (MQ=255)
                                                                                                                                 ggTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                    >  2:551799/1‑150 (MQ=255)
                                                                                                                                 agTCCTTTTAAAAGCTCAACAATTGCGGATCTTTTATGGATACTGATGCTGACGTTTAATATAAATAGAATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                    >  2:735328/2‑150 (MQ=255)
                                                                                                                                     gccataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                >  2:896955/8‑150 (MQ=255)
                                                                                                                                     cTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                >  2:301211/1‑150 (MQ=255)
                                                                                                                                      ttttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAt               <  1:474756/150‑1 (MQ=255)
                                                                                                                                       tttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  2:1032625/1‑150 (MQ=255)
                                                                                                                                       tttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  1:507035/1‑150 (MQ=255)
                                                                                                                                       cataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  1:1531510/6‑150 (MQ=255)
                                                                                                                                       cataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  1:1193294/6‑150 (MQ=255)
                                                                                                                                          acacGCTCAGCAATTGCTGAGCGTTTATATCTCCTGATTCTCACTATCAAGAAAAAAAAAATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCAGCCTGATTGAATATAGACATCAcc           >  2:1179007/1‑149 (MQ=255)
                                                                                                                                          acacGCTCAGCAATGGCGGAGCGTTTATGTCTCCTGATTCTGACGGTCAAGATAAAAAGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           >  2:421698/1‑150 (MQ=255)
                                                                                                                                          acacGCTCAGCAATGGCGGAGCGTTTATGTCTCCTGATGCTGACGTTGACGATAAATGTCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           >  2:1050678/1‑150 (MQ=255)
                                                                                                                                          acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           <  2:39657/150‑1 (MQ=255)
                                                                                                                                          acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           <  1:325927/150‑1 (MQ=255)
                                                                                                                                          acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACCGTGACGATAAATGCCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           >  2:385136/1‑150 (MQ=255)
                                                                                                                                             cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct        >  1:1509148/1‑150 (MQ=255)
                                                                                                                                             cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct        >  1:304849/1‑150 (MQ=255)
                                                                                                                                             cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct        >  1:1353432/1‑150 (MQ=255)
                                                                                                                                               cTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgc      <  1:990875/150‑1 (MQ=255)
                                                                                                                                                tCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCATCGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgcc     <  1:518643/150‑1 (MQ=255)
                                                                                                                                                   gCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCa  >  2:1107989/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGGTACCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCA  >  NC_000913/2775986‑2776282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑