Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,776,138 G→T 8.6% G74C (GGC→TGC)  yfjX → CP4‑57 prophage; putative antirestriction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,776,1380GT 8.6% 293.3 / 9.0 89G74C (GGC→TGC) yfjXCP4‑57 prophage; putative antirestriction protein
Reads supporting (aligned to +/- strand):  ref base G (54/27);  new base T (5/3);  total (59/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCA  >  NC_000913/2775990‑2776282
                                                                                                                                                    |                                                                                                                                                
aCCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATgg                                                                                                                                                 >  2:22164/1‑150 (MQ=255)
   cGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCgg                                                                                                                                              >  1:210650/1‑150 (MQ=255)
         aCAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCCGCCATTGCTGAGCGtg                                                                                                                                        <  2:233144/150‑2 (MQ=255)
            gCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTAt                                                                                                                                     >  1:606972/1‑150 (MQ=255)
              atatCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTTATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCCGCCATTGCTGAGCGTGTaaaa                                                                                                                                   <  2:1458573/150‑4 (MQ=37)
               tatCCGCTTCTGTCCGCAGCACTTTGGCACCATCCCGCAGTGTATAACGCTGTAAACGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTTGTCCTTTTACACGCTCCGCCATTGCTGAGCGTGTaaaag                                                                                                                                  <  2:1001048/150‑5 (MQ=37)
               tatCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGc                                                                                                                                  >  2:766187/1‑150 (MQ=255)
                atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGCGCGTTTATGGcg                                                                                                                                 >  2:1428115/1‑149 (MQ=255)
                atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCt                                                                                                                                 >  1:713584/1‑150 (MQ=255)
                atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCt                                                                                                                                 >  2:760076/1‑150 (MQ=255)
                  ccGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTcc                                                                                                                               <  2:568060/150‑1 (MQ=255)
                   cGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCt                                                                                                                              <  2:1467398/150‑1 (MQ=255)
                       tCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATg                                                                                                                          >  2:1331657/1‑150 (MQ=255)
                        cTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGc                                                                                                                         >  2:1111244/1‑150 (MQ=255)
                        cTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGc                                                                                                                         <  1:1002521/150‑1 (MQ=255)
                               cagcaCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGt                                                                                                                  <  2:213589/150‑1 (MQ=255)
                               cagcaCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGt                                                                                                                  <  2:919464/150‑1 (MQ=255)
                                  caCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGAc                                                                                                               >  1:39657/1‑150 (MQ=255)
                                  caCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGAc                                                                                                               <  1:1329444/150‑1 (MQ=255)
                                         gCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAAt                                                                                                        >  1:1431381/1‑150 (MQ=255)
                                          cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCg                                                                                                                                          >  2:559417/1‑115 (MQ=255)
                                          cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCg                                                                                                                                          <  1:559417/115‑1 (MQ=255)
                                          cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATg                                                                                                       >  1:475449/1‑150 (MQ=255)
                                          cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATg                                                                                                       <  2:683174/150‑1 (MQ=255)
                                            ccATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGc                                                                                                     >  1:1156472/1‑150 (MQ=255)
                                            ccATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGc                                                                                                     <  2:713584/150‑1 (MQ=255)
                                               tCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCAtt                                                                                                  >  1:788834/1‑150 (MQ=255)
                                                  cGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGc                                                                                               <  1:1111244/150‑1 (MQ=255)
                                                   gCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCt                                                                                              >  2:981504/1‑150 (MQ=255)
                                                    cAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTc                                                                                             <  1:283633/150‑1 (MQ=255)
                                                     aGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCa                                                                                            >  1:508457/1‑150 (MQ=255)
                                                       tGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGGGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAAc                                                                                          >  1:1492971/1‑150 (MQ=255)
                                                        ggATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACg                                                                                         >  2:16045/1‑150 (MQ=255)
                                                        ggATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCGCAAcc                                                                                         >  2:683021/1‑149 (MQ=255)
                                                          aTAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGc                                                                                       >  1:481189/1‑150 (MQ=255)
                                                           tAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGAc                                                                                                                     <  1:1082825/119‑1 (MQ=255)
                                                           tAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGAc                                                                                                                     >  2:1082825/1‑119 (MQ=255)
                                                           tAACGCTCGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGCTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCa                                                                                      >  2:43818/1‑150 (MQ=255)
                                                             aCGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATg                                                                                    >  2:246951/1‑150 (MQ=255)
                                                                cTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAAt                                                                                 >  1:1453546/1‑150 (MQ=255)
                                                                     aCCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCaa                                                                            >  1:911967/1‑150 (MQ=255)
                                                                     aCCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCaa                                                                            >  1:566782/1‑150 (MQ=255)
                                                                       cgcgCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATg                                                                          >  1:900685/1‑150 (MQ=255)
                                                                       cgcgCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATg                                                                          >  2:1294687/1‑150 (MQ=255)
                                                                             ttttCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCgg                                                                    >  2:582462/1‑150 (MQ=255)
                                                                             ttttCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCgg                                                                    >  2:1374133/1‑150 (MQ=255)
                                                                                     tggatggaCCGCTTCTGTGATGAGTACTTCGGGGGTATCTGGTCCTTTTACACCCTCAGCAATGGCGGAGCGTTTATCGCTCCTGATGCTGACGGTGACGATAAATGGCATTTCCTCAACGGCATGAATGTCAATCGTGACGAAATAAgc                                                            >  2:1156381/1‑150 (MQ=37)
                                                                                      ggatggaCCGCTTCTGGGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAgcg                                                           <  1:747292/150‑1 (MQ=255)
                                                                                       gatggaCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAgcgc                                                          >  1:203495/1‑150 (MQ=255)
                                                                                         tggaCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCgg                                                        >  1:754289/1‑150 (MQ=255)
                                                                                            aCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAg                                                     >  2:902711/1‑150 (MQ=255)
                                                                                               gCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCg                                                  >  2:571756/1‑150 (MQ=255)
                                                                                                cTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGc                                                 <  2:754289/150‑1 (MQ=255)
                                                                                                 ttCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCa                                                <  2:1302466/150‑1 (MQ=255)
                                                                                                  tCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAg                                               >  1:724232/1‑150 (MQ=255)
                                                                                                     gtgATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTa                                            >  1:1114685/1‑150 (MQ=255)
                                                                                                     gtgATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTa                                            <  2:1431381/150‑1 (MQ=255)
                                                                                                      tgatgaGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTAt                                           <  1:766187/150‑1 (MQ=255)
                                                                                                            gTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGt                                     >  2:742543/1‑150 (MQ=255)
                                                                                                               cTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTg                                  >  2:56166/1‑150 (MQ=255)
                                                                                                                tGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGc                                 >  1:712627/1‑150 (MQ=255)
                                                                                                                 gCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGcc                                <  2:1453546/150‑1 (MQ=255)
                                                                                                                      ggTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                           <  2:210650/150‑1 (MQ=255)
                                                                                                                      ggTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                           <  1:16045/150‑1 (MQ=255)
                                                                                                                        tATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGa                         >  1:508930/1‑150 (MQ=255)
                                                                                                                          tCTGGTCCTTTTACACGCGCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAat                       <  2:1219877/150‑1 (MQ=255)
                                                                                                                           cTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                      >  2:1408473/1‑150 (MQ=255)
                                                                                                                           cTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                      <  2:788834/150‑1 (MQ=255)
                                                                                                                            tGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAatat                     >  2:714439/1‑150 (MQ=255)
                                                                                                                             ggTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                    >  2:551799/1‑150 (MQ=255)
                                                                                                                             agTCCTTTTAAAAGCTCAACAATTGCGGATCTTTTATGGATACTGATGCTGACGTTTAATATAAATAGAATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                    >  2:735328/2‑150 (MQ=255)
                                                                                                                                 gccataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                >  2:896955/8‑150 (MQ=255)
                                                                                                                                 cTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                >  2:301211/1‑150 (MQ=255)
                                                                                                                                  ttttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAt               <  1:474756/150‑1 (MQ=255)
                                                                                                                                   tttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  2:1032625/1‑150 (MQ=255)
                                                                                                                                   tttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  1:507035/1‑150 (MQ=255)
                                                                                                                                   cataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  1:1531510/6‑150 (MQ=255)
                                                                                                                                   cataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc              >  1:1193294/6‑150 (MQ=255)
                                                                                                                                      acacGCTCAGCAATTGCTGAGCGTTTATATCTCCTGATTCTCACTATCAAGAAAAAAAAAATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCAGCCTGATTGAATATAGACATCAcc           >  2:1179007/1‑149 (MQ=255)
                                                                                                                                      acacGCTCAGCAATGGCGGAGCGTTTATGTCTCCTGATTCTGACGGTCAAGATAAAAAGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           >  2:421698/1‑150 (MQ=255)
                                                                                                                                      acacGCTCAGCAATGGCGGAGCGTTTATGTCTCCTGATGCTGACGTTGACGATAAATGTCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           >  2:1050678/1‑150 (MQ=255)
                                                                                                                                      acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           <  2:39657/150‑1 (MQ=255)
                                                                                                                                      acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           <  1:325927/150‑1 (MQ=255)
                                                                                                                                      acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACCGTGACGATAAATGCCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg           >  2:385136/1‑150 (MQ=255)
                                                                                                                                         cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct        >  1:1353432/1‑150 (MQ=255)
                                                                                                                                         cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct        >  1:1509148/1‑150 (MQ=255)
                                                                                                                                         cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct        >  1:304849/1‑150 (MQ=255)
                                                                                                                                           cTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgc      <  1:990875/150‑1 (MQ=255)
                                                                                                                                            tCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCATCGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgcc     <  1:518643/150‑1 (MQ=255)
                                                                                                                                               gCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCa  >  2:1107989/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACCCGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCA  >  NC_000913/2775990‑2776282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑