Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,776,141 G→T 7.7% G75* (GGA→TGA)  yfjX → CP4‑57 prophage; putative antirestriction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,776,1410GT 7.7% 287.3 / 8.3 88G75* (GGA→TGA) yfjXCP4‑57 prophage; putative antirestriction protein
Reads supporting (aligned to +/- strand):  ref base G (54/27);  new base T (4/3);  total (58/30)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

CGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGC  >  NC_000913/2775993‑2776290
                                                                                                                                                    |                                                                                                                                                     
cGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCgg                                                                                                                                                      >  1:210650/1‑150 (MQ=255)
      aCAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCCGCCATTGCTGAGCGtg                                                                                                                                                <  2:233144/150‑2 (MQ=255)
         gCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTAt                                                                                                                                             >  1:606972/1‑150 (MQ=255)
           atatCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTTATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCCGCCATTGCTGAGCGTGTaaaa                                                                                                                                           <  2:1458573/150‑4 (MQ=37)
            tatCCGCTTCTGTCCGCAGCACTTTGGCACCATCCCGCAGTGTATAACGCTGTAAACGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTTGTCCTTTTACACGCTCCGCCATTGCTGAGCGTGTaaaag                                                                                                                                          <  2:1001048/150‑5 (MQ=37)
            tatCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGc                                                                                                                                          >  2:766187/1‑150 (MQ=255)
             atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGCGCGTTTATGGcg                                                                                                                                         >  2:1428115/1‑149 (MQ=255)
             atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCt                                                                                                                                         >  1:713584/1‑150 (MQ=255)
             atCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCt                                                                                                                                         >  2:760076/1‑150 (MQ=255)
               ccGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTcc                                                                                                                                       <  2:568060/150‑1 (MQ=255)
                cGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCt                                                                                                                                      <  2:1467398/150‑1 (MQ=255)
                    tCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATg                                                                                                                                  >  2:1331657/1‑150 (MQ=255)
                     cTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGc                                                                                                                                 <  1:1002521/150‑1 (MQ=255)
                     cTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGc                                                                                                                                 >  2:1111244/1‑150 (MQ=255)
                            cagcaCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGt                                                                                                                          <  2:213589/150‑1 (MQ=255)
                            cagcaCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGt                                                                                                                          <  2:919464/150‑1 (MQ=255)
                               caCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGAc                                                                                                                       >  1:39657/1‑150 (MQ=255)
                               caCTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGAc                                                                                                                       <  1:1329444/150‑1 (MQ=255)
                                      gCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAAt                                                                                                                >  1:1431381/1‑150 (MQ=255)
                                       cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCg                                                                                                                                                  >  2:559417/1‑115 (MQ=255)
                                       cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCg                                                                                                                                                  <  1:559417/115‑1 (MQ=255)
                                       cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATg                                                                                                               <  2:683174/150‑1 (MQ=255)
                                       cACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATg                                                                                                               >  1:475449/1‑150 (MQ=255)
                                         ccATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGc                                                                                                             >  1:1156472/1‑150 (MQ=255)
                                         ccATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGc                                                                                                             <  2:713584/150‑1 (MQ=255)
                                            tCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCAtt                                                                                                          >  1:788834/1‑150 (MQ=255)
                                               cGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGc                                                                                                       <  1:1111244/150‑1 (MQ=255)
                                                gCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCt                                                                                                      >  2:981504/1‑150 (MQ=255)
                                                 cAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTc                                                                                                     <  1:283633/150‑1 (MQ=255)
                                                  aGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCa                                                                                                    >  1:508457/1‑150 (MQ=255)
                                                    tGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGGGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAAc                                                                                                  >  1:1492971/1‑150 (MQ=255)
                                                     ggATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACg                                                                                                 >  2:16045/1‑150 (MQ=255)
                                                     ggATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCGCAAcc                                                                                                 >  2:683021/1‑149 (MQ=255)
                                                       aTAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGc                                                                                               >  1:481189/1‑150 (MQ=255)
                                                        tAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGAc                                                                                                                             >  2:1082825/1‑119 (MQ=255)
                                                        tAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGAc                                                                                                                             <  1:1082825/119‑1 (MQ=255)
                                                        tAACGCTCGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGCTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCa                                                                                              >  2:43818/1‑150 (MQ=255)
                                                          aCGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATg                                                                                            >  2:246951/1‑150 (MQ=255)
                                                             cTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAAt                                                                                         >  1:1453546/1‑150 (MQ=255)
                                                                  aCCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCaa                                                                                    >  1:911967/1‑150 (MQ=255)
                                                                  aCCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCaa                                                                                    >  1:566782/1‑150 (MQ=255)
                                                                    cgcgCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATg                                                                                  >  1:900685/1‑150 (MQ=255)
                                                                    cgcgCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATg                                                                                  >  2:1294687/1‑150 (MQ=255)
                                                                          ttttCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCgg                                                                            >  2:1374133/1‑150 (MQ=255)
                                                                          ttttCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCgg                                                                            >  2:582462/1‑150 (MQ=255)
                                                                                  tggatggaCCGCTTCTGTGATGAGTACTTCGGGGGTATCTGGTCCTTTTACACCCTCAGCAATGGCGGAGCGTTTATCGCTCCTGATGCTGACGGTGACGATAAATGGCATTTCCTCAACGGCATGAATGTCAATCGTGACGAAATAAgc                                                                    >  2:1156381/1‑150 (MQ=37)
                                                                                   ggatggaCCGCTTCTGGGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAgcg                                                                   <  1:747292/150‑1 (MQ=255)
                                                                                    gatggaCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAgcgc                                                                  >  1:203495/1‑150 (MQ=255)
                                                                                      tggaCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCgg                                                                >  1:754289/1‑150 (MQ=255)
                                                                                         aCCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAg                                                             >  2:902711/1‑150 (MQ=255)
                                                                                            gCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCg                                                          >  2:571756/1‑150 (MQ=255)
                                                                                             cTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGc                                                         <  2:754289/150‑1 (MQ=255)
                                                                                              ttCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCa                                                        <  2:1302466/150‑1 (MQ=255)
                                                                                               tCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAg                                                       >  1:724232/1‑150 (MQ=255)
                                                                                                  gtgATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTa                                                    >  1:1114685/1‑150 (MQ=255)
                                                                                                  gtgATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTa                                                    <  2:1431381/150‑1 (MQ=255)
                                                                                                   tgatgaGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTAt                                                   <  1:766187/150‑1 (MQ=255)
                                                                                                         gTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGt                                             >  2:742543/1‑150 (MQ=255)
                                                                                                            cTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTg                                          >  2:56166/1‑150 (MQ=255)
                                                                                                             tGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGc                                         >  1:712627/1‑150 (MQ=255)
                                                                                                              gCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGcc                                        <  2:1453546/150‑1 (MQ=255)
                                                                                                                   ggTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                                   <  2:210650/150‑1 (MQ=255)
                                                                                                                   ggTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                                   <  1:16045/150‑1 (MQ=255)
                                                                                                                     tATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGa                                 >  1:508930/1‑150 (MQ=255)
                                                                                                                       tCTGGTCCTTTTACACGCGCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAat                               <  2:1219877/150‑1 (MQ=255)
                                                                                                                        cTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                              >  2:1408473/1‑150 (MQ=255)
                                                                                                                        cTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                              <  2:788834/150‑1 (MQ=255)
                                                                                                                         tGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAatat                             >  2:714439/1‑150 (MQ=255)
                                                                                                                          ggTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                            >  2:551799/1‑150 (MQ=255)
                                                                                                                          agTCCTTTTAAAAGCTCAACAATTGCGGATCTTTTATGGATACTGATGCTGACGTTTAATATAAATAGAATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                            >  2:735328/2‑150 (MQ=255)
                                                                                                                              gccataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                        >  2:896955/8‑150 (MQ=255)
                                                                                                                              cTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                        >  2:301211/1‑150 (MQ=255)
                                                                                                                               ttttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAt                       <  1:474756/150‑1 (MQ=255)
                                                                                                                                tttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                      >  1:507035/1‑150 (MQ=255)
                                                                                                                                tttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                      >  2:1032625/1‑150 (MQ=255)
                                                                                                                                cataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                      >  1:1531510/6‑150 (MQ=255)
                                                                                                                                cataaacGCTCCGCCATTGCTGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                      >  1:1193294/6‑150 (MQ=255)
                                                                                                                                   acacGCTCAGCAATTGCTGAGCGTTTATATCTCCTGATTCTCACTATCAAGAAAAAAAAAATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCAGCCTGATTGAATATAGACATCAcc                   >  2:1179007/1‑149 (MQ=255)
                                                                                                                                   acacGCTCAGCAATGGCGGAGCGTTTATGTCTCCTGATTCTGACGGTCAAGATAAAAAGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg                   >  2:421698/1‑150 (MQ=255)
                                                                                                                                   acacGCTCAGCAATGGCGGAGCGTTTATGTCTCCTGATGCTGACGTTGACGATAAATGTCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg                   >  2:1050678/1‑150 (MQ=255)
                                                                                                                                   acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg                   <  1:325927/150‑1 (MQ=255)
                                                                                                                                   acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg                   <  2:39657/150‑1 (MQ=255)
                                                                                                                                   acacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACCGTGACGATAAATGCCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACg                   >  2:385136/1‑150 (MQ=255)
                                                                                                                                      cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct                >  1:1509148/1‑150 (MQ=255)
                                                                                                                                      cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct                >  1:304849/1‑150 (MQ=255)
                                                                                                                                      cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct                >  1:1353432/1‑150 (MQ=255)
                                                                                                                                        cTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgc              <  1:990875/150‑1 (MQ=255)
                                                                                                                                         tCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCATCGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgcc             <  1:518643/150‑1 (MQ=255)
                                                                                                                                            gCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCa          >  2:1107989/1‑150 (MQ=255)
                                                                                                                                                    ggAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGc  <  1:760076/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATGAACAGCATATCCGCTTCTGGCCGCAGCACTTTGGCACCATCCCGCAGTGGATAACGCTGGAACCGCGCATTTTCGCCTGGATGGACCGCTTCTGTGATGAGTACTGCGGGGGTATCTGGTCCTTTTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGC  >  NC_000913/2775993‑2776290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑