Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,780,552 G→A 6.0% G725G (GGC→GGT)  ypjA ← adhesin‑like autotransporter YpjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,780,5520GA 6.0% 254.1 / 3.4 82G725G (GGC→GGT) ypjAadhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (42/34);  new base A (3/2);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTACCATAATT  >  NC_000913/2780408‑2780701
                                                                                                                                                |                                                                                                                                                     
gTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTgccgcc                                                                                                                                                  >  1:121294/1‑150 (MQ=255)
    gccgcACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTGCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAACGCGCCCTGGCTACTCTGCGGTCTCCCCGTTGCGCGGCCTTTCTCCCGCACCTCGCGTGTGCCGCCGTcg                                                                                                                                              >  2:1520529/1‑149 (MQ=255)
       gcACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTa                                                                                                                                           <  1:910183/150‑1 (MQ=255)
        cACCCTGCGCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGGGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTaa                                                                                                                                          <  2:722173/150‑1 (MQ=255)
           ccTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCa                                                                                                                                       <  2:153683/150‑1 (MQ=255)
              gCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTCGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCg                                                                                                                                    >  1:147004/1‑150 (MQ=255)
                tCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGCCCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCCCagag                                                                                                                                  >  1:1167949/1‑150 (MQ=255)
                tCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAg                                                                                                                                  <  2:919499/150‑1 (MQ=255)
                 cGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGt                                                                                                                                 >  1:1257768/1‑150 (MQ=255)
                   aTGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGttt                                                                                                                               <  1:1118882/150‑1 (MQ=255)
                      cTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCa                                                                                                                            <  2:121294/150‑1 (MQ=255)
                       tGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAg                                                                                                                           <  1:640884/150‑1 (MQ=255)
                       tGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAg                                                                                                                           <  1:1168998/150‑1 (MQ=255)
                       tGAACGCGACGCCATCCGCGCGTGTTACTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAg                                                                                                                           >  2:659011/1‑150 (MQ=255)
                        gAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGc                                                                                                                          >  1:325178/1‑150 (MQ=255)
                        gAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGc                                                                                                                          <  2:234354/150‑1 (MQ=255)
                             cgACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGgc                                                                                                                     <  2:928594/150‑1 (MQ=255)
                             cgACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGgc                                                                                                                     >  1:188509/1‑150 (MQ=255)
                                   cATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCt                                                                                                               >  1:258038/1‑150 (MQ=255)
                                    aTCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTc                                                                                                              <  1:1363206/150‑1 (MQ=255)
                                       cgcgcgTGTTCCTGTGACCCGCGTCGCCCTGGTGGGTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCAtt                                                                                                           <  2:160190/150‑1 (MQ=255)
                                        gcgcgTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTa                                                                                                          <  1:1481670/150‑1 (MQ=255)
                                         cgcgTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTaa                                                                                                         <  2:1257768/150‑1 (MQ=255)
                                          gcgTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCACACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAAc                                                                                                        <  1:607482/150‑1 (MQ=255)
                                                 ccTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCtgt                                                                                                 >  2:987239/1‑150 (MQ=255)
                                                 ccTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCCAGTTTTCAGCCAGGCCGCCCTCATTAACTACCtgt                                                                                                 >  2:114515/1‑150 (MQ=255)
                                                      gACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTCCTCCCGCACATCGAGTGTACCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAAcc                                                                                            >  2:1427482/1‑150 (MQ=255)
                                                      gACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTCCTCCCGCACATCGAGTGTACCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAAcc                                                                                            >  2:1422182/1‑150 (MQ=255)
                                                        cccGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAAcccc                                                                                          >  1:1529523/1‑150 (MQ=255)
                                                         ccGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAAccccc                                                                                         >  1:622046/1‑150 (MQ=255)
                                                               cGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATtaat                                                                                   >  2:135175/1‑150 (MQ=255)
                                                                  ccTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTaataat                                                                                >  1:401772/1‑150 (MQ=255)
                                                                    tggtggTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATgg                                                                              >  2:11272/1‑150 (MQ=255)
                                                                        ggtTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAAcc                                                                          >  1:1392609/1‑150 (MQ=255)
                                                                         gtTGCAACCAACGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGACCCCCCATTAATAATGGAACCt                                                                         >  2:1329498/1‑150 (MQ=255)
                                                                          ttGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTACCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCtt                                                                        >  2:1050787/1‑150 (MQ=255)
                                                                            gCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTcc                                                                      <  1:706481/150‑1 (MQ=255)
                                                                              aaCCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGc                                                                    <  1:390525/150‑1 (MQ=255)
                                                                                ccAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTg                                                                  >  1:455269/1‑150 (MQ=255)
                                                                                  aaaGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTc                                                                <  2:568126/150‑1 (MQ=255)
                                                                                   aaGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTcc                                                               <  1:444569/150‑1 (MQ=255)
                                                                                    aGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTccc                                                              >  1:185585/1‑150 (MQ=255)
                                                                                     gcgcCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCATCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCg                                                             >  1:179563/1‑150 (MQ=255)
                                                                                        cccTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGttt                                                          <  2:622046/150‑1 (MQ=255)
                                                                                          cTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGc                                                        >  2:881514/1‑150 (MQ=255)
                                                                                           tGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGcc                                                       <  2:258038/150‑1 (MQ=255)
                                                                                             gCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCAt                                                     <  2:465011/150‑1 (MQ=255)
                                                                                               tACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATaa                                                   >  1:994781/1‑150 (MQ=255)
                                                                                                aCTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGGCGTCATTAACTACCTGTGAACCCCCATTAATAATGGAAGCTTCCGCTGTCCCGTTTGCCATAAt                                                  <  1:800164/150‑1 (MQ=255)
                                                                                                aCTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAAt                                                  >  1:611833/1‑150 (MQ=255)
                                                                                                 ctctGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAAtt                                                 >  2:336060/1‑150 (MQ=255)
                                                                                                     gctgTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATttgtt                                             >  1:740093/1‑150 (MQ=255)
                                                                                                       tgTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTtgttgt                                           >  2:1274836/1‑150 (MQ=255)
                                                                                                         tATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAg                                         <  2:347596/150‑1 (MQ=255)
                                                                                                         tATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAg                                         <  1:1329498/150‑1 (MQ=255)
                                                                                                         tATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAg                                         <  1:457732/150‑1 (MQ=255)
                                                                                                            ccccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAGGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGcc                                      >  2:465026/1‑150 (MQ=255)
                                                                                                            ccccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGcc                                      <  2:147004/150‑1 (MQ=255)
                                                                                                            ccccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGcc                                      <  2:994781/150‑1 (MQ=255)
                                                                                                            ccccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGcc                                      >  2:1145836/1‑150 (MQ=255)
                                                                                                            ccccGTTGCGCTGCCTTCCTCCCGCACATCGAGTGTACCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGcc                                      <  1:1422182/150‑1 (MQ=255)
                                                                                                            ccccGTTGCGCTGCCTTCCTCCCGCACATCGAGTGTACCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGcc                                      <  1:1427482/150‑1 (MQ=255)
                                                                                                             cccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCa                                     >  1:929687/1‑150 (MQ=255)
                                                                                                              ccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTTCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCag                                    <  1:1050787/150‑1 (MQ=255)
                                                                                                              ccGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCag                                    <  1:1482170/150‑1 (MQ=255)
                                                                                                                    cgcTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTCCCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGa                              >  1:985192/1‑150 (MQ=255)
                                                                                                                     gcTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAgaga                                 >  1:722766/1‑146 (MQ=255)
                                                                                                                     gcTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAgaga                                 <  2:722766/146‑1 (MQ=255)
                                                                                                                     gcTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGat                             >  1:314947/1‑150 (MQ=255)
                                                                                                                          cTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCgg                        <  2:1312261/150‑1 (MQ=255)
                                                                                                                              ctcCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTgtt                    <  1:386384/150‑1 (MQ=255)
                                                                                                                              ctcCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTatt                    >  1:351581/1‑150 (MQ=255)
                                                                                                                              ctcCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCACCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTatt                    >  1:1092478/1‑150 (MQ=255)
                                                                                                                                cccGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATCTCGGTattga                  >  2:889039/1‑150 (MQ=255)
                                                                                                                                cccGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTattga                  >  1:1122729/1‑150 (MQ=255)
                                                                                                                                cccGCACATCGAGTGTGCCGCCGTCATTAAGCAACGAGTTTTAAGCCAGGCCGCCCTCATTAACTACCAGTGAACCCCCATTAATAATGGAACCTTACGCTGTCCCGTTTGCCATAATTTCTTGTAGGCCAGAGACGATATCGGTattga                  >  1:1255857/1‑150 (MQ=255)
                                                                                                                                 ccGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGCACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTattgat                 >  1:713148/1‑150 (MQ=255)
                                                                                                                                  cGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTattgatt                >  2:454702/1‑150 (MQ=255)
                                                                                                                                    cacaTCGAGTGTGCCGCCGTCATTAGGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGATATTGATTGc              <  1:465026/150‑1 (MQ=255)
                                                                                                                                      caTCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCt            >  1:372413/1‑150 (MQ=255)
                                                                                                                                       aTCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCtt           <  1:1133915/150‑1 (MQ=255)
                                                                                                                                        tCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTa          >  2:975467/1‑150 (MQ=255)
                                                                                                                                          gAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTAcc        >  2:149396/1‑150 (MQ=255)
                                                                                                                                             tgtgCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATTGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTACCATa     <  1:262287/150‑1 (MQ=255)
                                                                                                                                               tgCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTACCATAAt   <  2:188509/150‑1 (MQ=255)
                                                                                                                                                gccgccGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTTCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTACCATAAtt  <  1:616154/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTTCCGCTGTCCCGTTTGCCATAATTTGTTGTAGGCCAGAGACGATATCGGTATTGATTGCCTTACCATAATT  >  NC_000913/2780408‑2780701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑