Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,008,630 T→C 6.3% I374T (ATC→ACC)  ygeX → 2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,008,6300TC 6.3% 237.5 / 4.6 79I374T (ATC→ACC) ygeX2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (40/34);  new base C (3/2);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTAAGAATATTC  >  NC_000913/3008482‑3008779
                                                                                                                                                    |                                                                                                                                                     
ggTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATc                                                                                                                                                      <  1:211420/150‑1 (MQ=255)
   aaTCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGc                                                                                                                                                   >  2:1351262/1‑150 (MQ=255)
     tCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCAc                                                                                                                                                 <  1:1031223/150‑1 (MQ=255)
     tCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCAc                                                                                                                                                 <  1:10083/150‑1 (MQ=255)
     tCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCAc                                                                                                                                                 >  2:1230883/1‑150 (MQ=255)
     tCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCAc                                                                                                                                                 <  1:105973/150‑1 (MQ=255)
          aCGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCCTGGTGCTGGTTATCACCACTGaac                                                                                                                                            >  2:1297559/1‑149 (MQ=255)
          aCGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGGTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGATAACCAGCAccacgg                                                                                                                                            <  1:790112/150‑6 (MQ=255)
            ggCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGc                                                                                                                                          <  1:1094564/150‑1 (MQ=255)
             gCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCg                                                                                                                                         <  2:1203914/150‑1 (MQ=255)
             gCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCg                                                                                                                                         >  1:888517/1‑150 (MQ=255)
              cAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGa                                                                                                                                        >  2:1182486/1‑150 (MQ=255)
               aaCGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGac                                                                                                                                       <  2:319452/150‑1 (MQ=255)
                  gACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACAcc                                                                                                                                    <  2:618480/150‑1 (MQ=255)
                      cgcgCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACg                                                                                                                                <  1:226560/150‑1 (MQ=255)
                         gcATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGa                                                                                                                             <  2:380806/150‑1 (MQ=255)
                         gcATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGa                                                                                                                             <  2:1034993/150‑1 (MQ=255)
                           atcatcTCCGGTGAATCCGGCGCTGTCCGTTTGGGCGTTATCGCAGCGGATCATTAACACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAAAAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAg                                                                                                                           >  2:22312/1‑150 (MQ=255)
                            tcatcTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGc                                                                                                                          <  1:1529036/150‑1 (MQ=255)
                              atcTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGACGCAc                                                                                                                        >  1:1086385/1‑150 (MQ=255)
                              atcTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCAc                                                                                                                        >  1:1233958/1‑150 (MQ=255)
                               tctcCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACt                                                                                                                       >  2:764742/1‑150 (MQ=255)
                                  ccGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTAcc                                                                                                                    >  1:10809/1‑150 (MQ=255)
                                    ggTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTGATTATCAGCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAAGAAAGATGCCGTGGTGCTGGTTTTCAGCACTGAAGTCGACACCGACGTGAAGCACTACggc                                                                                                                  <  2:1520332/150‑1 (MQ=255)
                                             ggCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTc                                                                                                         <  1:433476/150‑1 (MQ=255)
                                             ggCGCTGTCGGATTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGACGCACTACCGCGAAGTTGTc                                                                                                         >  2:436118/1‑150 (MQ=255)
                                                   gTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAATGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGaa                                                                                                   >  1:1115925/1‑150 (MQ=255)
                                                     cgttttGGTCGTTCTCGCAGAGGTTAATTATAACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAgg                                                                                                 >  2:750536/4‑150 (MQ=255)
                                                       ttttGGGCGTTCTCGCAGCGGTTCATTATCACCCTCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTTCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCa                                                                                               <  1:660412/149‑1 (MQ=255)
                                                       gTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCTCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCa                                                                                               <  1:656227/150‑1 (MQ=255)
                                                        tttGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCATAACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCaa                                                                                              <  2:962347/150‑1 (MQ=255)
                                                           gggCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGAGAAGTTGTCTGGGAAGGCAAaca                                                                                           >  2:1260304/1‑150 (MQ=255)
                                                              cGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGc                                                                                        <  2:10809/150‑1 (MQ=255)
                                                                 gctcGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGt                                                                                     <  1:330546/149‑1 (MQ=255)
                                                                  ctcGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTa                                                                                    >  2:173898/1‑150 (MQ=255)
                                                                    cGCAGCGGTTCATTATCCCCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCAGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGc                                                                                  >  2:863708/1‑150 (MQ=255)
                                                                    cGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGc                                                                                                                          >  2:854921/1‑110 (MQ=255)
                                                                    cGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGc                                                                                                                          <  1:854921/110‑1 (MQ=255)
                                                                        gCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGTCAAACACGCTGTAGCACCt                                                                              <  2:888517/150‑1 (MQ=255)
                                                                         cGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCtt                                                                             <  1:1182486/150‑1 (MQ=255)
                                                                           gTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTaa                                                                           >  1:584769/1‑150 (MQ=255)
                                                                           gTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTaa                                                                           >  1:544756/1‑150 (MQ=255)
                                                                             tCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATc                                                                         <  2:1153126/150‑1 (MQ=255)
                                                                               aTTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCAc                                                                       >  1:687156/1‑150 (MQ=255)
                                                                                ttATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCAcc                                                                      >  2:962733/1‑150 (MQ=255)
                                                                                ttATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCAcc                                                                      >  2:557755/1‑150 (MQ=255)
                                                                                 tATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCAccc                                                                     >  2:1153175/1‑150 (MQ=255)
                                                                                    cACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTAt                                                                  >  1:1291122/1‑150 (MQ=255)
                                                                                     aaacccAACTTCAAAGCATGATCGAAAAACTGGATATGAACAAATATGCCGTGGTGATGGTTATCAGCAATGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTAtt                                                                 >  2:450862/6‑150 (MQ=255)
                                                                                        cGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGa                                                              <  1:1230883/150‑1 (MQ=255)
                                                                                         ccAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGaa                                                             <  2:1045941/149‑1 (MQ=255)
                                                                                          cAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAAc                                                            >  2:1493819/1‑150 (MQ=255)
                                                                                               tCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTc                                                       <  1:1422623/150‑1 (MQ=255)
                                                                                                 aaaGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTccc                                                     >  1:204631/1‑150 (MQ=255)
                                                                                                  aaGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCt                                                    >  1:840249/1‑150 (MQ=255)
                                                                                                   aGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTg                                                   <  2:1367451/150‑1 (MQ=255)
                                                                                                       tGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGCCCCCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCaaa                                               >  2:237660/1‑150 (MQ=255)
                                                                                                       tGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCaaa                                               >  1:1303627/1‑150 (MQ=255)
                                                                                                        gATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCaaaa                                              >  2:1457251/1‑150 (MQ=255)
                                                                                                            gAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACggg                                          >  2:98299/1‑150 (MQ=255)
                                                                                                             aaaaaCTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACgggg                                         >  1:75904/1‑150 (MQ=255)
                                                                                                             aaaaaCTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACgggg                                         <  1:750536/150‑1 (MQ=255)
                                                                                                             aaaaaCTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACgggg                                         <  2:544756/150‑1 (MQ=255)
                                                                                                                     gcgcTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTaaaaa                                 >  1:28188/1‑150 (MQ=255)
                                                                                                                      cgcTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCTACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTaaaaaa                                >  2:1219777/1‑150 (MQ=255)
                                                                                                                      cgcTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTaaaaaa                                >  2:485120/1‑150 (MQ=255)
                                                                                                                       gcTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAAt                               <  1:173898/150‑1 (MQ=255)
                                                                                                                        cTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATc                              <  2:30365/150‑1 (MQ=255)
                                                                                                                          gAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGCAGGCGCCCCCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTCGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTg                            >  1:694310/1‑150 (MQ=255)
                                                                                                                             cAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGgag                         <  2:1182374/150‑1 (MQ=255)
                                                                                                                                  aTGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGaaaaa                    >  2:1538064/1‑150 (MQ=255)
                                                                                                                                   tGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAaat                   <  1:1263937/150‑1 (MQ=255)
                                                                                                                                   tGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCAGGAGAAAaat                   >  2:636486/1‑150 (MQ=255)
                                                                                                                                   gccttcagtgCTGATAACCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAaat                   >  1:519262/8‑150 (MQ=255)
                                                                                                                                    ccttcagtgCTGATAACCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAaata                  >  1:1442077/7‑150 (MQ=255)
                                                                                                                                     cttcagtgCTGATAACCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTgg                           >  1:1279610/6‑140 (MQ=255)
                                                                                                                                     cttcagtgCTGATAACCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTgg                           <  2:1279610/135‑1 (MQ=255)
                                                                                                                                           tgCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTa           <  1:1457251/150‑1 (MQ=255)
                                                                                                                                             cTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTAAg         >  2:950740/1‑150 (MQ=255)
                                                                                                                                                    tCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTAAGAATATTc  <  1:863708/150‑1 (MQ=255)
                                                                                                                                                    tCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTAAGAATATTc  <  1:557755/150‑1 (MQ=255)
                                                                                                                                                    tCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTAAGAATATTc  <  1:300817/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACGTGAAGCACTACCGCGAAGTTGTCTGGGAAGGCAAACACGCTGTAGCACCTTAATCACCCTATTGGAACTGCTCCCTGCAAAACGGGGAGTAAAAAATCTGGAGAAAAATAATGGCTAAGAATATTC  >  NC_000913/3008482‑3008779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑