Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,477,878	T→A	8.6%	intergenic (+20/+29)	slyX → / ← slyD	protein SlyX/FKBP‑type peptidyl‑prolyl cis‑trans isomerase SlyD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,878	0	T	A	8.6%	198.2 / 7.0	69	intergenic (+20/+29)	slyX/slyD	protein SlyX/FKBP‑type peptidyl‑prolyl cis‑trans isomerase SlyD
Reads supporting (aligned to +/- strand):  ref base T (31/32);  new base A (4/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

GACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGG  >  NC_000913/3477732‑3478024                                                                                                                                                   |                                                                                                                                                   caCCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTcc                                                                                                                                                 >  1:1494356/2‑150 (MQ=255)   ccGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTcccc                                                                                                                                               <  1:1420859/150‑1 (MQ=255)         aTGAAATGGAGATGGCGACACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttt                                                                                                                                         <  1:1061148/150‑1 (MQ=255)         aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                         <  2:806809/150‑1 (MQ=255)          tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                        <  2:391911/150‑1 (MQ=255)            aaaTGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttttt                                                                                                                                      <  2:645652/150‑2 (MQ=255)              aTGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTc                                                                                                                                    <  2:1507642/150‑1 (MQ=255)               tGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCa                                                                                                                                   <  2:498456/150‑1 (MQ=255)                 gagaTGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACt                                                                                                                                 >  2:753307/1‑150 (MQ=255)                     tGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGTGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACttttt                                                                                                                             >  2:987499/1‑150 (MQ=255)                       gCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCg                                                                                                                           >  1:438820/1‑150 (MQ=255)                       gCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCg                                                                                                                           <  2:343118/150‑1 (MQ=255)                        cGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCgg                                                                                                                          >  2:1158677/1‑150 (MQ=255)                          aaaCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTa                                                                                                                        >  1:1390910/1‑150 (MQ=255)                           aaCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                        >  2:714701/1‑133 (MQ=255)                           aaCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                        <  1:714701/133‑1 (MQ=255)                            aCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTAtt                                                                                                                      <  2:1309734/150‑1 (MQ=255)                            aCTGCGCGATCATATGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTAtt                                                                                                                      <  2:96891/150‑1 (MQ=255)                              tGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAg                                                                                                                    <  2:1133995/150‑1 (MQ=255)                              tGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAg                                                                                                                    <  2:438820/150‑1 (MQ=255)                                cgcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTg                                                                                                                  <  1:70582/150‑1 (MQ=255)                                 gcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTgg                                                                                                                 <  1:349814/150‑1 (MQ=255)                                 gcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTgg                                                                                                                 <  2:804402/150‑1 (MQ=255)                                              tCTGCTGACCGAGAAGTTAAAAGCCAGCCAACCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCaaccgcaaccgc                                                                                                    >  1:1532448/1‑150 (MQ=255)                                                    gACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGACACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGc                                                                                              >  2:998924/1‑150 (MQ=255)                                                     aCCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGcc                                                                                             >  2:1011021/1‑150 (MQ=255)                                                     aCCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGcc                                                                                             >  1:97979/1‑150 (MQ=255)                                                      ccGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTATTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCt                                                                                            >  1:1391285/1‑150 (MQ=255)                                                       cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCtt                                                                                           <  2:1140824/150‑1 (MQ=255)                                                       cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCtt                                                                                           <  2:1302581/150‑1 (MQ=255)                                                          gaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAc                                                                                        >  1:1010585/1‑150 (MQ=255)                                                           aaGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGTTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAcc                                                                                       <  1:998924/150‑1 (MQ=255)                                                            aGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTATCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCg                                                                                      >  2:512647/1‑150 (MQ=255)                                                               tAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCca                                                                                   >  2:255487/1‑150 (MQ=255)                                                                aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCcac                                                                                  <  1:544651/150‑1 (MQ=255)                                                                     ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcagcag                                                                             <  1:1401078/150‑1 (MQ=255)                                                                     ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcagcag                                                                             <  1:546311/150‑1 (MQ=255)                                                                      cagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACagcagc                                                                            >  1:1359504/1‑150 (MQ=255)                                                                                 cGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCg                                                                 <  2:1010585/150‑1 (MQ=255)                                                                                  gAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgt                                                                >  2:459201/1‑150 (MQ=255)                                                                                     cATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt                                                             <  2:749785/150‑1 (MQ=255)                                                                                      aTCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTc                                                            <  2:97979/150‑1 (MQ=255)                                                                                       tCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCa                                                           >  2:234129/1‑150 (MQ=255)                                                                                       tCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCa                                                           >  1:496008/1‑150 (MQ=255)                                                                                         gcgTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTTGGTTTTAGTGGCAAGCGCAACCGCCGTTGCCTTTACCGCCACAGCAGTCTTCGCCACCGTGTTGAtt                                                         <  2:1494356/150‑2 (MQ=255)                                                                                               cAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTg                                                   <  2:51073/150‑1 (MQ=255)                                                                                                  gCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATc                                                >  2:1169489/1‑150 (MQ=255)                                                                                                       agaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGc                                           >  2:1028689/1‑150 (MQ=255)                                                                                                        gaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGcc                                          >  2:124835/1‑150 (MQ=255)                                                                                                            cgccaccgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGa                                      <  2:415449/150‑1 (MQ=255)                                                                                                            cgccaccgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCCGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCAAGa                                      >  2:227448/1‑150 (MQ=255)                                                                                                                    ccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgca                              <  2:384891/150‑1 (MQ=255)                                                                                                                     cacaTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgcag                             >  2:162977/1‑150 (MQ=255)                                                                                                                     cacaTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgcag                             <  2:880795/150‑1 (MQ=255)                                                                                                                       caTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgcagca                           <  2:891763/150‑1 (MQ=255)                                                                                                                              gAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcgtcg                    >  2:24156/1‑150 (MQ=255)                                                                                                                              gAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcgtcg                    >  2:959052/1‑150 (MQ=255)                                                                                                                                ggCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtg                  >  2:209523/1‑150 (MQ=255)                                                                                                                                   gTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtc               >  1:1198769/1‑150 (MQ=255)                                                                                                                                    acaaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAGCCgtcgtggtcg              >  1:1326401/3‑150 (MQ=255)                                                                                                                                     caaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt             >  1:441689/2‑150 (MQ=255)                                                                                                                                     caaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt             >  1:916937/2‑150 (MQ=255)                                                                                                                                     aaaaaaaGCGGGGATTCGCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCTCCGCAGCAACCgtcgtggtcgt             <  1:512647/150‑1 (MQ=255)                                                                                                                                     aaaaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTAATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt             >  2:1028157/1‑150 (MQ=255)                                                                                                                                      aaaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgtg            >  1:1493795/1‑150 (MQ=255)                                                                                                                                      aaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgtg            >  2:118593/1‑150 (MQ=255)                                                                                                                                           aGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgt       >  2:493259/1‑150 (MQ=255)                                                                                                                                             cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtgg     <  1:255487/150‑1 (MQ=255)                                                                                                                                                ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCGTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCTCAGCAACCGTCGTGGTCGTGATCgtggtgg  <  1:1314816/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGG  >  NC_000913/3477732‑3478024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑