BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,529,744	G→T	5.1%	E133* (GAA→TAA)	hslR →	heat shock protein Hsp15

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,529,744	0	G	T	5.1%	334.9 / 5.6	98	E133* (GAA→TAA)	hslR	heat shock protein Hsp15
Reads supporting (aligned to +/- strand): ref base G (47/46); new base T (2/3); total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 103 total aligned reads displayed.

TGCTGTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCC  >  NC_000913/3529595‑3529893

tGCTGTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTg                                                                                                                                                       <  2:1346125/150‑1 (MQ=255)

tGCTGTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTg                                                                                                                                                       <  1:1350303/150‑1 (MQ=255)

tGCTGTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTg                                                                                                                                                       <  2:370849/150‑1 (MQ=255)

   tGTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAAt                                                                                                                                                    <  1:422180/150‑1 (MQ=255)

    gTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATa                                                                                                                                                   <  2:767456/150‑1 (MQ=255)

    gTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGACGCACCCGGAACGACGCCCGGACAAAAAAGAGCGACGCGACCTGTTACGATTTAAACACGTCGACAGTGAATa                                                                                                                                                   >  2:517293/1‑150 (MQ=255)

    gTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGACACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATa                                                                                                                                                   <  1:1440167/150‑1 (MQ=255)

     tATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGCCGCCCGGACAAAAAAGAGCGCCGCGACCTCTTACGATTTAAACACGGCGACAGTGAATaa                                                                                                                                                  >  2:495558/1‑150 (MQ=255)

      aTGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAAc                                                                                                                                                 >  2:483488/1‑150 (MQ=255)

       tGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACt                                                                                                                                                >  2:208947/1‑150 (MQ=255)

        gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                               >  2:32918/1‑150 (MQ=255)

        gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                               <  1:238659/150‑1 (MQ=255)

        gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                               <  1:208947/150‑1 (MQ=255)

        gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                               <  1:874400/150‑1 (MQ=255)

        gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                               >  1:1314450/1‑150 (MQ=255)

          agagACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTc                                                                                                                                             >  1:477283/1‑150 (MQ=255)

          agagACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTc                                                                                                                                             <  1:244242/150‑1 (MQ=255)

            agaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCAc                                                                                                                                           <  1:640130/150‑1 (MQ=255)

              aCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGTCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccg                                                                                                                                         <  1:34861/150‑2 (MQ=255)

              aCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccg                                                                                                                                         <  1:281500/150‑2 (MQ=255)

               cTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccgt                                                                                                                                        <  1:375741/150‑3 (MQ=255)

               cTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTg                                                                                                                                        >  1:1446464/1‑150 (MQ=255)

               cTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTg                                                                                                                                        >  1:981317/1‑150 (MQ=255)

                 gCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCa                                                                                                                                      >  2:1458506/1‑150 (MQ=255)

                  cGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCaa                                                                                                                                     >  2:401380/1‑150 (MQ=255)

                   ggAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAag                                                                                                                                    <  1:896448/150‑1 (MQ=255)

                       aGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAagagag                                                                                                                                >  2:809901/1‑150 (MQ=255)

                        gtgtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAgagaga                                                                                                                               >  1:1500919/1‑150 (MQ=255)

                        gtgtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAgagaga                                                                                                                               >  1:1436220/1‑150 (MQ=255)

                          gtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgatg                                                                                                                             <  2:1314450/150‑1 (MQ=255)

                            agagAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgatgat                                                                                                                           <  2:566669/150‑1 (MQ=255)

                               gaAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTAt                                                                                                                        >  2:289583/1‑150 (MQ=255)

                                 aaCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGc                                                                                                                      <  1:1271485/150‑1 (MQ=255)

                                 aaCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGc                                                                                                                      >  2:1317299/1‑150 (MQ=255)

                                   cgcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCg                                                                                                                    >  2:955545/1‑150 (MQ=255)

                                    gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGc                                                                                                                   <  1:809901/150‑1 (MQ=255)

                                    gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGc                                                                                                                   >  2:80986/1‑150 (MQ=255)

                                     cgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGACGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCa                                                                                                                  >  2:1228111/1‑150 (MQ=255)

                                        aaaaaTGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACa                                                                                                               <  1:269615/150‑1 (MQ=255)

                                          aaaTGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATg                                                                                                             <  1:80986/150‑1 (MQ=255)

                                          aaaTGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATg                                                                                                             <  2:1161641/150‑1 (MQ=255)

                                             tGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGTCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGAcc                                                                                                          <  2:806485/150‑1 (MQ=255)

                                                 gcTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                      <  2:1535077/150‑1 (MQ=255)

                                                 gcTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                      <  2:895429/150‑1 (MQ=255)

                                                 gcTGGCACGTAAACATAATGCCTTAACCATGCCGCACCCGGGCCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                      <  2:435693/150‑1 (MQ=255)

                                                   tGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTAc                                                                                                    <  2:670020/150‑1 (MQ=255)

                                                    ggCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACa                                                                                                   >  1:1393676/1‑150 (MQ=255)

                                                      cACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATc                                                                                                 >  2:700211/1‑150 (MQ=255)

                                                      cACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATc                                                                                                 >  2:1055050/1‑150 (MQ=255)

                                                        cGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGc                                                                                               <  2:43985/150‑1 (MQ=255)

                                                          tAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTa                                                                                             >  2:1248308/1‑150 (MQ=255)

                                                           aaaCTTAATGCCTTAACCATGCCGCAACCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGACGCAACATGACCAATTACATCGCTAt                                                                                            >  1:77561/1‑150 (MQ=255)

                                                            aaCTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATc                                                                                           >  1:242861/1‑150 (MQ=255)

                                                                 aaTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGttt                                                                                      >  1:588454/1‑150 (MQ=255)

                                                                  aTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTg                                                                                     <  1:1138189/150‑1 (MQ=255)

                                                                  aTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTg                                                                                     <  2:1475832/150‑1 (MQ=255)

                                                                   tGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGa                                                                                    <  1:1513345/150‑1 (MQ=255)

                                                                          aCCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACttt                                                                             >  1:594185/1‑150 (MQ=255)

                                                                            cATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGc                                                                           >  2:284268/1‑150 (MQ=255)

                                                                            cATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGc                                                                           <  1:1317299/150‑1 (MQ=255)

                                                                               gCCACACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGt                                                                        >  2:624965/1‑150 (MQ=255)

                                                                                ccGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTg                                                                       >  2:1019878/1‑150 (MQ=255)

                                                                                     cccGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcgg                                                                  >  2:189426/1‑150 (MQ=255)

                                                                                     cccGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcgg                                                                  <  1:1055050/150‑1 (MQ=255)

                                                                                       cGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcggcg                                                                >  2:1011621/1‑150 (MQ=255)

                                                                                        ggACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGa                                                               <  1:289583/150‑1 (MQ=255)

                                                                                           ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                            >  2:1288511/1‑150 (MQ=255)

                                                                                           ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                            <  1:158933/150‑1 (MQ=255)

                                                                                           ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                            <  1:32918/150‑1 (MQ=255)

                                                                                            cGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTg                                                           <  1:514757/150‑1 (MQ=255)

                                                                                              aCGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGt                                                         <  1:587053/150‑1 (MQ=255)

                                                                                               cGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTa                                                        <  1:1288511/150‑1 (MQ=255)

                                                                                                       cAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTc                                                >  2:696022/1‑150 (MQ=255)

                                                                                                        aaaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCg                                               <  2:941564/150‑1 (MQ=255)

                                                                                                         aaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAAGTTTCCCGGGCGCGGCGAAATGGAACCCGTTGCgg                                              >  2:953406/1‑150 (MQ=255)

                                                                                                           aaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGaa                                            <  1:1349798/150‑1 (MQ=255)

                                                                                                               agCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAAccc                                        <  1:401380/150‑1 (MQ=255)

                                                                                                                  gccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGc                                     >  1:1507482/1‑150 (MQ=255)

                                                                                                                   ccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCa                                    >  2:1206395/1‑150 (MQ=255)

                                                                                                                   ccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCa                                    >  2:934651/1‑150 (MQ=255)

                                                                                                                      cgACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACa                                 <  1:1011621/150‑1 (MQ=255)

                                                                                                                        aCCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGa                               <  2:981317/150‑1 (MQ=255)

                                                                                                                               aCGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTga                        <  1:867793/150‑1 (MQ=255)

                                                                                                                                cGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgag                       >  2:167727/1‑150 (MQ=255)

                                                                                                                                 gATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgaga                      <  1:1435795/150‑1 (MQ=255)

                                                                                                                                 gATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgaga                      >  2:1367182/1‑150 (MQ=255)

                                                                                                                                      aaaCACGGCGACAGTGCATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCAc                 <  1:1325422/150‑1 (MQ=255)

                                                                                                                                       aaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACg                >  1:402829/1‑150 (MQ=255)

                                                                                                                                       aaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACg                >  1:604560/1‑150 (MQ=255)

                                                                                                                                          caggtgACAGTTATTCACTGTCGCCTGCAAGAGAGATGATTATTCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCAGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGAtt             >  2:201637/6‑150 (MQ=255)

                                                                                                                                          caggtgACAGTTATTCACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGAtt             >  2:1248905/6‑150 (MQ=255)

                                                                                                                                           cGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTa            >  2:228165/1‑150 (MQ=255)

                                                                                                                                              cGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGAATATcc         <  2:594424/150‑1 (MQ=255)

                                                                                                                                               gACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCg        >  2:1398183/1‑150 (MQ=255)

                                                                                                                                                  aGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAg     >  1:86063/1‑150 (MQ=255)

                                                                                                                                                   ttGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTTAAAACTTTGCCGTGCGCGGCTAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGc    <  1:517293/149‑1 (MQ=255)

                                                                                                                                                   gTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGc    >  1:1424836/1‑150 (MQ=255)

                                                                                                                                                    tGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGcc   <  2:242861/150‑1 (MQ=255)

                                                                                                                                                     gAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGccc  >  1:523324/1‑150 (MQ=255)

                                                                                                                                                     gAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACACTGCAACAGATCCTTGAGAACCACGATTATCCGCAGccc  >  1:926934/1‑150 (MQ=255)

TGCTGTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCC  >  NC_000913/3529595‑3529893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`