BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,529,748	A→C	5.2%	*134S (TAA→TCA)	hslR →	heat shock protein Hsp15

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,529,748	0	A	C	5.2%	307.0 / 7.1	97	*134S (TAA→TCA)	hslR	heat shock protein Hsp15
Reads supporting (aligned to +/- strand): ref base A (48/44); new base C (2/3); total (50/47)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

Only 100 of 102 total aligned reads displayed.

GTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTA  >  NC_000913/3529599‑3529897

gTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATa                                                                                                                                                       <  2:767456/150‑1 (MQ=255)

gTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGACGCACCCGGAACGACGCCCGGACAAAAAAGAGCGACGCGACCTGTTACGATTTAAACACGTCGACAGTGAATa                                                                                                                                                       >  2:517293/1‑150 (MQ=255)

gTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGACACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATa                                                                                                                                                       <  1:1440167/150‑1 (MQ=255)

 tATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGCCGCCCGGACAAAAAAGAGCGCCGCGACCTCTTACGATTTAAACACGGCGACAGTGAATaa                                                                                                                                                      >  2:495558/1‑150 (MQ=255)

  aTGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAAc                                                                                                                                                     >  2:483488/1‑150 (MQ=255)

   tGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACt                                                                                                                                                    >  2:208947/1‑150 (MQ=255)

    gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                                   >  1:1314450/1‑150 (MQ=255)

    gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                                   >  2:32918/1‑150 (MQ=255)

    gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                                   <  1:874400/150‑1 (MQ=255)

    gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                                   <  1:238659/150‑1 (MQ=255)

    gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                                   <  1:208947/150‑1 (MQ=255)

      agagACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTc                                                                                                                                                 >  1:477283/1‑150 (MQ=255)

      agagACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTc                                                                                                                                                 <  1:244242/150‑1 (MQ=255)

        agaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCAc                                                                                                                                               <  1:640130/150‑1 (MQ=255)

          aCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGTCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccg                                                                                                                                             <  1:34861/150‑2 (MQ=255)

          aCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccg                                                                                                                                             <  1:281500/150‑2 (MQ=255)

           cTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccgt                                                                                                                                            <  1:375741/150‑3 (MQ=255)

           cTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTg                                                                                                                                            >  1:1446464/1‑150 (MQ=255)

             gCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCa                                                                                                                                          >  2:1458506/1‑150 (MQ=255)

              cGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCaa                                                                                                                                         >  2:401380/1‑150 (MQ=255)

               ggAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAag                                                                                                                                        <  1:896448/150‑1 (MQ=255)

                   aGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAagagag                                                                                                                                    >  2:809901/1‑150 (MQ=255)

                    gtgtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAgagaga                                                                                                                                   >  1:1500919/1‑150 (MQ=255)

                    gtgtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAgagaga                                                                                                                                   >  1:1436220/1‑150 (MQ=255)

                      gtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgatg                                                                                                                                 <  2:1314450/150‑1 (MQ=255)

                        agagAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgatgat                                                                                                                               <  2:566669/150‑1 (MQ=255)

                           gaAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTAt                                                                                                                            >  2:289583/1‑150 (MQ=255)

                             aaCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGc                                                                                                                          <  1:1271485/150‑1 (MQ=255)

                             aaCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGc                                                                                                                          >  2:1317299/1‑150 (MQ=255)

                               cgcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCg                                                                                                                        >  2:955545/1‑150 (MQ=255)

                                gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGc                                                                                                                       <  1:809901/150‑1 (MQ=255)

                                gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGc                                                                                                                       >  2:80986/1‑150 (MQ=255)

                                 cgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGACGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCa                                                                                                                      >  2:1228111/1‑150 (MQ=255)

                                    aaaaaTGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACa                                                                                                                   <  1:269615/150‑1 (MQ=255)

                                      aaaTGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATg                                                                                                                 <  2:1161641/150‑1 (MQ=255)

                                      aaaTGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATg                                                                                                                 <  1:80986/150‑1 (MQ=255)

                                         tGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGTCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGAcc                                                                                                              <  2:806485/150‑1 (MQ=255)

                                             gcTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                          <  2:895429/150‑1 (MQ=255)

                                             gcTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                          <  2:1535077/150‑1 (MQ=255)

                                             gcTGGCACGTAAACATAATGCCTTAACCATGCCGCACCCGGGCCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                          <  2:435693/150‑1 (MQ=255)

                                              cTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGTTTATGCCGCAACTTGACCAATTa                                                                                                         <  2:1500919/150‑1 (MQ=255)

                                               tGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTAc                                                                                                        <  2:670020/150‑1 (MQ=255)

                                                ggCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACa                                                                                                       >  1:1393676/1‑150 (MQ=255)

                                                  cACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATc                                                                                                     >  2:1055050/1‑150 (MQ=255)

                                                  cACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATc                                                                                                     >  2:700211/1‑150 (MQ=255)

                                                    cGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGc                                                                                                   <  2:43985/150‑1 (MQ=255)

                                                      tAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTa                                                                                                 >  2:1248308/1‑150 (MQ=255)

                                                       aaaCTTAATGCCTTAACCATGCCGCAACCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGACGCAACATGACCAATTACATCGCTAt                                                                                                >  1:77561/1‑150 (MQ=255)

                                                        aaCTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATc                                                                                               >  1:242861/1‑150 (MQ=255)

                                                             aaTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGttt                                                                                          >  1:588454/1‑150 (MQ=255)

                                                              aTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTg                                                                                         <  1:1138189/150‑1 (MQ=255)

                                                              aTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTg                                                                                         <  2:1475832/150‑1 (MQ=255)

                                                               tGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGa                                                                                        <  1:1513345/150‑1 (MQ=255)

                                                                   ttAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAAc                                                                                    <  1:483488/150‑1 (MQ=255)

                                                                      aCCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACttt                                                                                 >  1:594185/1‑150 (MQ=255)

                                                                        cATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGc                                                                               >  2:284268/1‑150 (MQ=255)

                                                                        cATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGc                                                                               <  1:1317299/150‑1 (MQ=255)

                                                                           gCCACACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGt                                                                            >  2:624965/1‑150 (MQ=255)

                                                                            ccGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTg                                                                           >  2:1019878/1‑150 (MQ=255)

                                                                                 cccGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcgg                                                                      >  2:189426/1‑150 (MQ=255)

                                                                                 cccGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcgg                                                                      <  1:1055050/150‑1 (MQ=255)

                                                                                   cGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcggcg                                                                    >  2:1011621/1‑150 (MQ=255)

                                                                                    ggACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGa                                                                   <  1:289583/150‑1 (MQ=255)

                                                                                       ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                                <  1:32918/150‑1 (MQ=255)

                                                                                       ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                                >  2:1288511/1‑150 (MQ=255)

                                                                                       ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                                <  1:158933/150‑1 (MQ=255)

                                                                                        cGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTg                                                               <  1:514757/150‑1 (MQ=255)

                                                                                          aCGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGt                                                             <  1:587053/150‑1 (MQ=255)

                                                                                           cGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTa                                                            <  1:1288511/150‑1 (MQ=255)

                                                                                                   cAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTc                                                    >  2:696022/1‑150 (MQ=255)

                                                                                                    aaaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCg                                                   <  2:941564/150‑1 (MQ=255)

                                                                                                     aaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAAGTTTCCCGGGCGCGGCGAAATGGAACCCGTTGCgg                                                  >  2:953406/1‑150 (MQ=255)

                                                                                                       aaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGaa                                                <  1:1349798/150‑1 (MQ=255)

                                                                                                           agCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAAccc                                            <  1:401380/150‑1 (MQ=255)

                                                                                                              gccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGc                                         >  1:1507482/1‑150 (MQ=255)

                                                                                                               ccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCa                                        >  2:1206395/1‑150 (MQ=255)

                                                                                                               ccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCa                                        >  2:934651/1‑150 (MQ=255)

                                                                                                                  cgACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACa                                     <  1:1011621/150‑1 (MQ=255)

                                                                                                                    aCCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGa                                   <  2:981317/150‑1 (MQ=255)

                                                                                                                           aCGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTga                            <  1:867793/150‑1 (MQ=255)

                                                                                                                            cGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgag                           >  2:167727/1‑150 (MQ=255)

                                                                                                                             gATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgaga                          >  2:1367182/1‑150 (MQ=255)

                                                                                                                             gATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgaga                          <  1:1435795/150‑1 (MQ=255)

                                                                                                                                  aaaCACGGCGACAGTGCATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCAc                     <  1:1325422/150‑1 (MQ=255)

                                                                                                                                   aaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACg                    >  1:604560/1‑150 (MQ=255)

                                                                                                                                   aaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACg                    >  1:402829/1‑150 (MQ=255)

                                                                                                                                      caggtgACAGTTATTCACTGTCGCCTGCAAGAGAGATGATTATTCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCAGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGAtt                 >  2:201637/6‑150 (MQ=255)

                                                                                                                                      caggtgACAGTTATTCACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGAtt                 >  2:1248905/6‑150 (MQ=255)

                                                                                                                                       cGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTa                >  2:228165/1‑150 (MQ=255)

                                                                                                                                          cGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGAATATcc             <  2:594424/150‑1 (MQ=255)

                                                                                                                                           gACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCg            >  2:1398183/1‑150 (MQ=255)

                                                                                                                                              aGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAg         >  1:86063/1‑150 (MQ=255)

                                                                                                                                               ttGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTTAAAACTTTGCCGTGCGCGGCTAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGc        <  1:517293/149‑1 (MQ=255)

                                                                                                                                               gTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGc        >  1:1424836/1‑150 (MQ=255)

                                                                                                                                                tGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGcc       <  2:242861/150‑1 (MQ=255)

                                                                                                                                                 gAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGccc      >  1:523324/1‑150 (MQ=255)

                                                                                                                                                 gAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACACTGCAACAGATCCTTGAGAACCACGATTATCCGCAGccc      >  1:926934/1‑150 (MQ=255)

                                                                                                                                                  aaTAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAACCCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCg     >  2:311690/1‑150 (MQ=255)

                                                                                                                                                    tAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGATATCTGTTTGAAAACTTTGCCGTGCGCTGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATTCTTGAGAACCACGATTATCCGCAGCCCGtt   <  2:1446464/150‑1 (MQ=255)

                                                                                                                                                     aaCTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTa  <  1:922945/150‑1 (MQ=255)

GTATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTA  >  NC_000913/3529599‑3529897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`