Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,647,412 A→T 5.4% K372* (AAA→TAA) ‡ gor → glutathione reductase (NADPH)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,647,4120AT 5.4% 286.0 / 5.3 92K372* (AAA→TAA) ‡gorglutathione reductase (NADPH)
Reads supporting (aligned to +/- strand):  ref base A (48/39);  new base T (2/3);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

Only 100 of 102 total aligned reads displayed.

GAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTG  >  NC_000913/3647265‑3647561
                                                                                                                                                   |                                                                                                                                                     
gAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTGCTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATaaa                                                                                                                                                     >  2:952793/1‑150 (MQ=255)
       tGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCtt                                                                                                                                              >  2:24297/1‑150 (MQ=255)
        gTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCttt                                                                                                                                             >  1:1002147/1‑150 (MQ=255)
          ttAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCa                                                                                                                                           >  1:648973/1‑150 (MQ=255)
          ttAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCa                                                                                                                                           >  1:520973/1‑150 (MQ=255)
           taataaCAATCCGGATGAGCATCTTGATTACAGCAACATTCCGACCGTGTTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCATGCTCGCGAGCAGTATGGCGACGATCAGGTTAAAGTTTATAAATCCTCTTTCAc                                                                                                                                          <  2:1313906/150‑1 (MQ=255)
           taataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCAc                                                                                                                                          >  2:873955/1‑150 (MQ=255)
           taataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCAc                                                                                                                                          >  2:750114/1‑150 (MQ=255)
           taataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCAc                                                                                                                                          <  1:627012/150‑1 (MQ=255)
             ataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGAGGATTTATACACTTTCAcct                                                                                                                                        <  1:363826/150‑2 (MQ=255)
              taaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACcgc                                                                                                                                       >  2:1244116/1‑150 (MQ=255)
               aaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGAGGATTTATACACTTTCAcctga                                                                                                                                      <  2:1521947/150‑4 (MQ=255)
               aaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGAGGATTTATACACTTTCAcctga                                                                                                                                      <  1:390821/150‑4 (MQ=255)
                 cAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGAt                                                                                                                                    >  1:1052733/1‑150 (MQ=255)
                      cGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGtata                                                                                                                               >  1:1411022/1‑150 (MQ=255)
                       ggATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATAc                                                                                                                              >  1:1488685/1‑150 (MQ=255)
                            aGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATAccgccg                                                                                                                         >  2:1198260/1‑150 (MQ=255)
                             gCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTCGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGt                                                                                                                        >  1:1534535/1‑150 (MQ=255)
                                tCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcac                                                                                                                     >  1:1307235/1‑150 (MQ=255)
                                 cTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcacc                                                                                                                    <  1:26965/150‑1 (MQ=255)
                                 cTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcacc                                                                                                                    <  1:1025812/150‑1 (MQ=255)
                                 cTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcacc                                                                                                                    >  1:149639/1‑150 (MQ=255)
                                   ggATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcaccac                                                                                                                  >  1:969467/1‑150 (MQ=255)
                                    gATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACt                                                                                                                 <  1:990929/150‑1 (MQ=255)
                                         cAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCg                                                                                                            >  1:924348/1‑150 (MQ=255)
                                         cAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCg                                                                                                            >  2:1108309/1‑150 (MQ=255)
                                           gCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGcc                                                                                                          >  2:265715/1‑150 (MQ=255)
                                            cAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCa                                                                                                         >  2:382608/1‑150 (MQ=255)
                                              aCATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCtt                                                                                                                                              <  1:1482407/111‑1 (MQ=255)
                                              aCATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCtt                                                                                                                                              >  2:1482407/1‑111 (MQ=255)
                                               cATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGcc                                                                                                      <  2:605255/150‑1 (MQ=255)
                                                    cGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGtata                                                                                                                                                       >  1:982727/1‑96 (MQ=255)
                                                    cGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGcc                                                                                                 >  2:944355/1‑150 (MQ=255)
                                                        cGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCAt                                                                                             <  1:783485/150‑1 (MQ=255)
                                                        cGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCAt                                                                                             <  1:1373709/150‑1 (MQ=255)
                                                         gTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCATGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATg                                                                                            <  2:888815/150‑1 (MQ=255)
                                                          tGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGa                                                                                           <  1:402618/150‑1 (MQ=255)
                                                           ggTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGaa                                                                                          >  2:1360196/1‑150 (MQ=255)
                                                            gTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGCTGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAg                                                                                         <  1:1347917/150‑1 (MQ=255)
                                                              cTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCGGCCGTGCCGCATGAAGCt                                                                                       >  2:801207/1‑150 (MQ=255)
                                                              cTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGc                                                                                        >  2:1137325/1‑149 (MQ=255)
                                                              cTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGc                                                                                        <  1:1137325/149‑1 (MQ=255)
                                                                  aGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGgtg                                                                                   >  2:1397335/1‑150 (MQ=255)
                                                                        ccgccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGtt                                                                             <  1:898917/150‑1 (MQ=255)
                                                                         cgccgATTGGTACTCGTGGTTTCACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTTGGGTGCGTTg                                                                            <  2:1032928/150‑1 (MQ=255)
                                                                             gATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATc                                                                        <  1:1244116/150‑1 (MQ=255)
                                                                             gATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATc                                                                        >  1:1235249/1‑150 (MQ=255)
                                                                              aTTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCg                                                                       <  1:1198260/150‑1 (MQ=255)
                                                                                tGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGga                                                                     >  1:1031696/1‑150 (MQ=255)
                                                                                 ggTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTAAGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaa                                                                    <  2:1145096/150‑1 (MQ=255)
                                                                                  gTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaag                                                                   <  2:52510/150‑1 (MQ=255)
                                                                                  gTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaag                                                                   <  2:520973/150‑1 (MQ=255)
                                                                                   tACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaaga                                                                  >  1:5383/1‑150 (MQ=255)
                                                                                    aCTGTTGGTTTGACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaagag                                                                 <  2:794518/150‑1 (MQ=255)
                                                                                    aCTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaagag                                                                 <  1:944355/150‑1 (MQ=255)
                                                                                       gTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaagagaag                                                              >  1:74353/1‑150 (MQ=255)
                                                                                       gTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaagagaag                                                              >  1:720650/1‑150 (MQ=255)
                                                                                           gTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTg                                                          >  1:453321/1‑150 (MQ=255)
                                                                                            tttAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACAGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATAGGAAGAGAAGATTGt                                                         >  1:1517405/1‑150 (MQ=255)
                                                                                               aaCGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCgg                                                      <  1:24297/150‑1 (MQ=255)
                                                                                               aaCGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCgg                                                      <  2:1535716/150‑1 (MQ=255)
                                                                                                aCGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGt                                                     >  2:1164078/1‑150 (MQ=255)
                                                                                                  ggAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGTATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTAt                                                   <  2:1130842/150‑1 (MQ=255)
                                                                                                     aCCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCa                                                >  1:710295/1‑150 (MQ=255)
                                                                                                      ccGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCAc                                               >  1:531557/1‑150 (MQ=255)
                                                                                                        gCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACgg                                             <  1:382608/150‑1 (MQ=255)
                                                                                                        gCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACgg                                             <  1:952793/150‑1 (MQ=255)
                                                                                                         cAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGc                                            >  1:583089/1‑150 (MQ=255)
                                                                                                             cgcgcgAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTg                                        <  1:873955/150‑1 (MQ=255)
                                                                                                               cgcgAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGc                                      >  1:1537954/1‑150 (MQ=255)
                                                                                                                 cgAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCtt                                    >  1:490275/1‑150 (MQ=255)
                                                                                                                   aGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTg                                  >  2:1480743/1‑150 (MQ=255)
                                                                                                                    gCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTgg                                 <  1:1189350/150‑1 (MQ=255)
                                                                                                                    gCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTgg                                 <  2:1411022/150‑1 (MQ=255)
                                                                                                                      aGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTa                               >  2:1218963/1‑150 (MQ=255)
                                                                                                                      aGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTa                               >  1:199410/1‑150 (MQ=255)
                                                                                                                       gTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTAt                              >  2:540492/1‑150 (MQ=255)
                                                                                                                        tATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATg                             <  1:1227885/150‑1 (MQ=255)
                                                                                                                         aTGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGTTGCATGAATTTTTTGTGCGTTGTATGTGATGAGAAGTTTTTGTGTATTCTTGGCATTTGGTTTGGTATgg                            <  2:418756/150‑1 (MQ=255)
                                                                                                                         aTGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATgg                            <  2:1523569/150‑1 (MQ=255)
                                                                                                                         aTGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATgg                            >  1:257007/1‑150 (MQ=255)
                                                                                                                           ggCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGAc                          >  1:69158/1‑150 (MQ=255)
                                                                                                                            gCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACg                         >  2:1234319/1‑150 (MQ=255)
                                                                                                                             cgacgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGa                        <  2:1031696/150‑1 (MQ=255)
                                                                                                                               acgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGaaa                      <  1:1108309/150‑1 (MQ=255)
                                                                                                                                 gaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATg                    <  1:1234319/150‑1 (MQ=255)
                                                                                                                                    cAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTg                 >  1:1367516/1‑150 (MQ=255)
                                                                                                                                     cggTGAAAGAGGATTTATACACTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGc                >  1:393963/2‑150 (MQ=255)
                                                                                                                                     cggTGAAAGAGGATTTATACACTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGc                >  1:964201/2‑150 (MQ=255)
                                                                                                                                     aGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGc                <  1:265715/150‑1 (MQ=255)
                                                                                                                                      ggTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCa               >  1:698565/1‑150 (MQ=255)
                                                                                                                                        tGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAgg             <  1:591375/150‑1 (MQ=255)
                                                                                                                                              tgtATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTcg       >  2:1333806/1‑150 (MQ=255)
                                                                                                                                                 ataAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCgg    <  1:693396/150‑1 (MQ=255)
                                                                                                                                                 ataAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCgg    >  1:220137/1‑150 (MQ=255)
                                                                                                                                                 ataAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCgg    >  2:329280/1‑150 (MQ=255)
                                                                                                                                                 ataAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCgg    >  1:140081/1‑150 (MQ=255)
                                                                                                                                                  taAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGt   >  1:1173441/1‑150 (MQ=255)
                                                                                                                                                  taAATCCTCTTTCACCGAAATGTATACCCCCTTCACAACTCACCGACAGCCGAGCATCATTAACATAAATTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGATATGTTGCAGGGCTTCGCGGt   >  2:399095/1‑150 (MQ=255)
                                                                                                                                                   aaaTCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGTTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTg  <  2:506412/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTG  >  NC_000913/3647265‑3647561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑