Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,692,676 C→A 5.4% R854L (CGC→CTC)  bcsA ← cellulose synthase catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,692,6760CA 5.4% 371.6 / 6.0 110R854L (CGC→CTC) bcsAcellulose synthase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (52/52);  new base A (4/2);  total (56/54)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 111 total aligned reads displayed.

GTCGCCTGCGTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCC  >  NC_000913/3692531‑3692825
                                                                                                                                                 |                                                                                                                                                     
gTCGCCTGCGTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCgg                                                                                                                                                   <  1:182784/150‑1 (MQ=255)
gTCGCCTGCGTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCgg                                                                                                                                                   <  2:1321915/150‑1 (MQ=255)
  cGCCTGCGTCATGAAAGAGGGGAGCGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGaa                                                                                                                                                 >  1:997365/1‑150 (MQ=255)
     cTGCGTCATGGAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCGCCGCCCGGAGCGGagcga                                                                                                                                              <  2:743644/150‑5 (MQ=255)
        cGTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACg                                                                                                                                           <  1:1187545/150‑1 (MQ=255)
         gTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGa                                                                                                                                          >  1:895456/1‑150 (MQ=255)
         gTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGa                                                                                                                                          <  1:860573/150‑1 (MQ=255)
           cATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATa                                                                                                                                        >  1:506580/1‑150 (MQ=255)
            aTGAAAGAGGGGAACGCACTCATTCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATAc                                                                                                                                       >  1:786072/1‑150 (MQ=255)
               aaaGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAAc                                                                                                                                    >  1:1397380/1‑150 (MQ=255)
                  gagGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCa                                                                                                                                 <  2:352021/150‑1 (MQ=255)
                   agGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAg                                                                                                                                <  1:1104214/150‑1 (MQ=255)
                    ggggAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAgg                                                                                                                               <  1:818778/150‑1 (MQ=255)
                      ggAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGGTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGGGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGaa                                                                                                                             <  1:1064164/150‑1 (MQ=255)
                          cGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAACCCa                                                                                                                         >  1:1454115/1‑150 (MQ=255)
                            cACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCaga                                                                                                                       >  2:83520/1‑150 (MQ=255)
                            cACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCaga                                                                                                                       >  1:457835/1‑150 (MQ=255)
                            cACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCaga                                                                                                                       >  2:496308/1‑150 (MQ=255)
                             aCTCATTCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAACCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCagag                                                                                                                      <  2:786072/150‑1 (MQ=255)
                             aCTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCagag                                                                                                                      <  2:1109491/150‑1 (MQ=255)
                             aCTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCagag                                                                                                                      <  2:413376/150‑1 (MQ=255)
                             aCTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTCAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCagag                                                                                                                      <  2:124678/150‑1 (MQ=255)
                                cATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAg                                                                                                                   >  2:1413635/1‑150 (MQ=255)
                                cATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAg                                                                                                                   >  1:66314/1‑150 (MQ=255)
                                  tCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTc                                                                                                                 >  1:1152843/1‑150 (MQ=255)
                                   ccccATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGTGAAGTCa                                                                                                                >  2:88561/1‑150 (MQ=255)
                                   ccccATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCa                                                                                                                >  2:186596/1‑150 (MQ=255)
                                    cccATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAg                                                                                                               >  1:301656/1‑150 (MQ=255)
                                    cccATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAg                                                                                                               >  1:675755/1‑150 (MQ=255)
                                      cATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGca                                                                                                             >  2:1229934/1‑150 (MQ=255)
                                           ccACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGTAACCAGAGAAGTCAGCACACgg                                                                                                        <  2:1268990/150‑1 (MQ=255)
                                           ccACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACgg                                                                                                        >  2:641974/1‑150 (MQ=255)
                                            cACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGa                                                                                                       >  1:1330025/1‑150 (MQ=255)
                                             aCTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATAc                                                                                                                                       <  1:1430002/117‑1 (MQ=255)
                                             aCTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATAc                                                                                                                                       >  2:1430002/1‑117 (MQ=255)
                                                  cacaaTCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGc                                                                                                 <  1:837363/147‑1 (MQ=255)
                                                   caAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTATCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGcc                                                                                                <  2:295720/150‑1 (MQ=255)
                                                     aaTCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCt                                                                                              >  1:1203633/1‑150 (MQ=255)
                                                      aTCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCtt                                                                                             <  1:1372879/150‑1 (MQ=255)
                                                          aGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCAcc                                                                                         >  2:20257/1‑150 (MQ=255)
                                                                  tttcttttcATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAgg                                                                                 >  2:195543/1‑150 (MQ=255)
                                                                  tttcttttcATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAgg                                                                                 <  1:1277074/150‑1 (MQ=255)
                                                                     cttttcATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCCCACGGAATATGCCCTTCACCGAAGAAGgcgc                                                                              >  2:1091371/1‑150 (MQ=255)
                                                                      ttttcATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCa                                                                             >  1:824339/1‑150 (MQ=255)
                                                                        ttcATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCaaa                                                                           >  2:466032/1‑150 (MQ=255)
                                                                           aTCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTc                                                                        >  1:769276/1‑150 (MQ=255)
                                                                            tCGCGTTATCATCATTGTTGAGCCACAGCCGGTTCGGATGGTTGGGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTcc                                                                       <  2:1306375/150‑1 (MQ=255)
                                                                            tCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCaa                                                                            <  1:1204403/145‑1 (MQ=255)
                                                                            tCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCaa                                                                            >  2:1204403/1‑145 (MQ=255)
                                                                            tCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTcc                                                                       >  2:270256/1‑150 (MQ=255)
                                                                                     catcatTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATgg                                                              >  2:1507861/1‑150 (MQ=255)
                                                                                      atcatTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCTAAGAAGGCGCAAACTCCGCCAGATggg                                                             <  2:52662/150‑2 (MQ=255)
                                                                                      atcatTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGc                                                             <  1:1068834/150‑1 (MQ=255)
                                                                                      atcatTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGc                                                             <  2:832018/150‑1 (MQ=255)
                                                                                       tcatTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAACCGATACACCCCAGGAAACCAGAGAAGTCAGCCCACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCg                                                            >  1:1134433/1‑150 (MQ=255)
                                                                                        catTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCgg                                                           <  2:1395850/150‑1 (MQ=255)
                                                                                         atTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGt                                                          >  1:1031356/1‑150 (MQ=255)
                                                                                            gttgAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGc                                                       >  2:715771/1‑150 (MQ=255)
                                                                                              tgACCCAAACCCCGAACCCCTGGTTGTGCCCTTTCGCTCCGCCCCCGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCATCTGGCggagtttg                                                     <  1:442588/150‑7 (MQ=255)
                                                                                                 gCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCCCACGGAATATGCCCTTCACCGAAGCAGGCGCAAACTCCGCCAGATGGCGGTAGCCACgg                                                  >  2:1511610/1‑150 (MQ=255)
                                                                                                 gCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACgg                                                  >  2:1348648/1‑150 (MQ=255)
                                                                                                   cAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGaa                                                >  1:1045291/1‑150 (MQ=255)
                                                                                                   cAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGaa                                                <  2:1397380/150‑1 (MQ=255)
                                                                                                    aaaGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAg                                               >  2:1157768/1‑150 (MQ=255)
                                                                                                      aGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGcc                                             <  1:853681/150‑1 (MQ=255)
                                                                                                         cTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAg                                          >  1:1221845/1‑150 (MQ=255)
                                                                                                         cTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAg                                          >  2:222659/1‑150 (MQ=255)
                                                                                                           gATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCt                                        <  2:804638/150‑1 (MQ=255)
                                                                                                           gATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCt                                        <  2:1456954/150‑1 (MQ=255)
                                                                                                            aTCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCtt                                       >  1:1279163/1‑150 (MQ=255)
                                                                                                              ccGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCa                                     <  2:457835/150‑1 (MQ=255)
                                                                                                               cGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAg                                    <  1:911960/150‑1 (MQ=255)
                                                                                                                gATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGa                                   >  2:1176531/1‑150 (MQ=255)
                                                                                                                gATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGa                                   <  1:1413635/150‑1 (MQ=255)
                                                                                                                  tGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAat                                 >  1:1268898/1‑150 (MQ=255)
                                                                                                                   ggTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAata                                <  1:1274266/150‑1 (MQ=255)
                                                                                                                     ttGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATc                              <  1:496308/150‑1 (MQ=255)
                                                                                                                      tgtgCCGTTTCACTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGGCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATcc                             <  1:830999/150‑1 (MQ=255)
                                                                                                                        tgCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCcag                           <  2:1222239/150‑1 (MQ=255)
                                                                                                                          ccGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGc                                              >  2:741020/1‑129 (MQ=255)
                                                                                                                          ccGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCcagca                         <  1:1283340/150‑1 (MQ=255)
                                                                                                                           cGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCcagcag                        >  2:681222/1‑150 (MQ=255)
                                                                                                                             tttCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGAc                      <  1:166411/150‑1 (MQ=255)
                                                                                                                              ttCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGTGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCAGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACt                     <  1:88561/150‑1 (MQ=255)
                                                                                                                              ttCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACt                     >  2:361667/1‑150 (MQ=255)
                                                                                                                               ccgcgccgcccGGAGCGGAGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACtt                    >  2:1131352/2‑150 (MQ=255)
                                                                                                                                cgctccgctcCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACttt                   <  2:301656/150‑1 (MQ=255)
                                                                                                                                cgctccgctcCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACttt                   >  2:72671/1‑150 (MQ=255)
                                                                                                                                cgcgccgcccGGAGCGGAGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCt                                        <  1:106493/129‑1 (MQ=255)
                                                                                                                                cgcgccgcccGGAGCGGAGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCt                                        >  2:106493/1‑129 (MQ=255)
                                                                                                                                 gctccgctccGGGCGGCGCGGAATAAACGATACCACCCAGGAAACCAGAGAAGTCAGCACACGTAATATGACCTTAACCGAAGAAGGCGCAAACTCCGGCAGATGGCGGTAGACACGGAAGCCGAGCTGCAGAATATCCAGCCGCCTTTc                  >  1:400915/1‑150 (MQ=255)
                                                                                                                                 gctccgctccGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTc                  >  2:1374805/1‑150 (MQ=255)
                                                                                                                                 attccgcgccgCCCGGAGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGCCTTTc                  >  2:504934/3‑150 (MQ=255)
                                                                                                                                   tccgcgccgCCCGGAGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCa                >  2:646032/1‑150 (MQ=255)
                                                                                                                                    ccgctccgGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAg               <  1:1265359/150‑1 (MQ=255)
                                                                                                                                     cgctccgGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGc              <  1:65511/150‑1 (MQ=255)
                                                                                                                                         ccgGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCt          <  1:1176531/150‑1 (MQ=255)
                                                                                                                                         ccgGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCt          >  1:900672/1‑150 (MQ=255)
                                                                                                                                           gggCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTa        <  1:757429/150‑1 (MQ=255)
                                                                                                                                                 cgcgGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTcc  <  1:1229934/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GTCGCCTGCGTCATGAAAGAGGGGAACGCACTCATCCCCATAGCCACTGCACAAATCCAGAATAGTTTTCTTTTCATCGCGTTATCATCATTGTTGAGCCAAAGCCTGATCCGATGGTTGTGCCGTTTCGCTCCGCTCCGGGCGGCGCGGAATAAACGATACAACCCAGGAAACCAGAGAAGTCAGCACACGGAATATGCCCTTCACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCC  >  NC_000913/3692531‑3692825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑