Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,975,732 A→C 6.2% D196A (GAT→GCT)  wecE → dTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,975,7320AC 6.2% 288.5 / 8.3 93D196A (GAT→GCT) wecEdTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase
Reads supporting (aligned to +/- strand):  ref base A (44/43);  new base C (4/2);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGC  >  NC_000913/3975583‑3975881
                                                                                                                                                     |                                                                                                                                                     
tttgtttgTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGa                                                                                                                                                       <  2:353579/150‑1 (MQ=255)
   gtatgtgGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAAACATTAGTCATATTGGCTGCTTTAGCTTCCATGAATCCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATaa                                                                                                                                                    >  2:871873/4‑150 (MQ=255)
      tgtgGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGc                                                                                                                                                 <  1:1179412/150‑1 (MQ=255)
         ggTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGtt                                                                                                                                              >  2:240377/1‑150 (MQ=255)
          gTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTa                                                                                                                                             >  2:465373/1‑150 (MQ=255)
            agaagaTGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAAt                                                                                                                                           <  1:110042/150‑1 (MQ=255)
               agaTGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGa                                                                                                                                        <  1:1188501/150‑1 (MQ=255)
                 aTGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAAc                                                                                                                                      <  2:452354/150‑1 (MQ=255)
                  tGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACg                                                                                                                                     >  2:1135536/1‑150 (MQ=255)
                  tGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACg                                                                                                                                     <  2:146193/150‑1 (MQ=255)
                      gCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGcc                                                                                                                                 >  2:1002133/1‑150 (MQ=255)
                              cGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGatcat                                                                                                                         >  2:382825/1‑150 (MQ=255)
                              cGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGatcat                                                                                                                         >  2:1518297/1‑150 (MQ=255)
                                 gATGTCCACTTACAAAGGGCGGGCACTGGGAACCAGTGGGCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGGGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCg                                                                                                                      <  2:1071402/150‑1 (MQ=255)
                                   tGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTg                                                                                                                    <  2:1087009/150‑1 (MQ=255)
                                   tGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTg                                                                                                                    <  1:39474/150‑1 (MQ=255)
                                    gTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGa                                                                                                                   >  1:486999/1‑150 (MQ=255)
                                     tCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGCTTTATCGTTAATc                                                                                                                                          >  1:776099/1‑126 (MQ=255)
                                     tCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGCTTTATCGTTAATc                                                                                                                                          <  2:776099/126‑1 (MQ=255)
                                     tCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGaa                                                                                                                  >  1:901729/1‑150 (MQ=255)
                                      ccACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGaaa                                                                                                                 >  1:1285737/1‑150 (MQ=255)
                                       cACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGaaaa                                                                                                                <  1:1420082/150‑1 (MQ=255)
                                         cTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAgg                                                                                                              <  2:1036342/150‑1 (MQ=255)
                                           tACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGc                                                                                                            >  2:407293/1‑150 (MQ=255)
                                           tACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGc                                                                                                            <  1:1135536/150‑1 (MQ=255)
                                             cAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCAc                                                                                                          >  2:614394/1‑150 (MQ=255)
                                             cAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCAc                                                                                                          >  2:626505/1‑150 (MQ=255)
                                             cAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCAc                                                                                                          <  1:917693/150‑1 (MQ=255)
                                               aaGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTa                                                                                                        >  2:994228/1‑150 (MQ=255)
                                                  ggCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAAcc                                                                                                     <  1:482945/150‑1 (MQ=255)
                                                   gCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGatca                                                                                                                          <  1:1012003/128‑1 (MQ=255)
                                                   gCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGatca                                                                                                                          >  2:1012003/1‑128 (MQ=255)
                                                      tGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAg                                                                                                 >  2:1381379/1‑150 (MQ=255)
                                                       gCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGc                                                                                                <  1:1493678/150‑1 (MQ=255)
                                                          cTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGAGCACTAACCGCAGCCAg                                                                                             >  2:643798/1‑150 (MQ=255)
                                                           tGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGt                                                                                            <  1:1168736/150‑1 (MQ=255)
                                                            gggAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGCTTTATCGTTaa                                                                                                                                            >  1:895080/1‑101 (MQ=255)
                                                            gggAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGCTTTATCGTTaa                                                                                                                                            <  2:895080/101‑1 (MQ=255)
                                                             ggAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGttc                                                                                          >  1:1094563/1‑150 (MQ=255)
                                                                  cATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCg                                                                                     <  2:1020885/150‑1 (MQ=255)
                                                                    ttGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTg                                                                                   <  1:323413/150‑1 (MQ=255)
                                                                     tGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTgg                                                                                  <  2:1130546/150‑1 (MQ=255)
                                                                      ggTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGCGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGt                                                                                 >  1:1043396/1‑150 (MQ=255)
                                                                      ggTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGt                                                                                 >  1:1216297/1‑150 (MQ=255)
                                                                        tCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCa                                                                               <  2:212501/150‑1 (MQ=255)
                                                                        tCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCa                                                                               <  2:1094563/150‑1 (MQ=255)
                                                                         cATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAg                                                                              >  2:1174174/1‑150 (MQ=255)
                                                                          atatTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAgg                                                                             >  2:973948/1‑150 (MQ=255)
                                                                           tatTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGTGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGt                                                                            >  1:818297/1‑150 (MQ=255)
                                                                               ggCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGAt                                                                        >  2:908416/1‑150 (MQ=255)
                                                                               ggCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGAt                                                                        >  2:199689/1‑150 (MQ=255)
                                                                                gctgctTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATa                                                                       <  1:382825/150‑1 (MQ=255)
                                                                                gctgctTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATa                                                                       <  2:1508159/150‑1 (MQ=255)
                                                                                 ctgctTTAGCTTCCATGAATCCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATaa                                                                      <  1:871873/150‑1 (MQ=255)
                                                                                  tgctTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATaaa                                                                     <  1:1301135/150‑1 (MQ=255)
                                                                                   gctTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAat                                                                    <  1:614394/150‑1 (MQ=255)
                                                                                       tAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATAc                                                                >  2:742890/1‑150 (MQ=255)
                                                                                       tAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATAc                                                                >  2:1037092/1‑150 (MQ=255)
                                                                                       tAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGATAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATAc                                                                >  2:604654/1‑150 (MQ=255)
                                                                                             ccATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGgcg                                                          >  2:1471805/1‑150 (MQ=255)
                                                                                              cATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGgcgc                                                         >  2:542387/1‑150 (MQ=255)
                                                                                                tGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGa                                                       <  2:56941/150‑1 (MQ=255)
                                                                                                 gAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGat                                                      >  1:534345/1‑150 (MQ=255)
                                                                                                    aCCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATAtt                                                   >  1:1502858/1‑150 (MQ=255)
                                                                                                     ccAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTg                                                  >  2:1397356/1‑150 (MQ=255)
                                                                                                     ccAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTg                                                  <  2:369010/150‑1 (MQ=255)
                                                                                                      cAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTgg                                                 <  1:742890/150‑1 (MQ=255)
                                                                                                          aaCTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTcc                                             <  1:931428/150‑1 (MQ=255)
                                                                                                               cacGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAat                                                                    <  1:1309836/122‑1 (MQ=255)
                                                                                                               cacGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAat                                                                    >  2:1309836/1‑122 (MQ=255)
                                                                                                                   gcgggcggTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGTGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTg                                    <  1:643798/150‑1 (MQ=255)
                                                                                                                    cgggcggTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGa                                   <  2:780831/150‑1 (MQ=255)
                                                                                                                          gTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCg                             >  2:1129690/1‑150 (MQ=255)
                                                                                                                          gTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCg                             >  1:1503701/1‑150 (MQ=255)
                                                                                                                              aGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCt                         >  1:1081790/1‑150 (MQ=255)
                                                                                                                              aGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCt                         <  1:973948/150‑1 (MQ=255)
                                                                                                                              aGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCt                         <  1:1172519/150‑1 (MQ=255)
                                                                                                                               ggcggcGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTg                        >  2:1503583/1‑150 (MQ=255)
                                                                                                                                 cggcgCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCa                      <  2:459210/150‑1 (MQ=255)
                                                                                                                                  ggcgCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCaa                     >  1:555281/1‑150 (MQ=255)
                                                                                                                                   gcgcGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAg                    <  2:119004/150‑1 (MQ=255)
                                                                                                                                    cgcgACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGc                   <  1:994228/150‑1 (MQ=255)
                                                                                                                                      cgACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTg                 >  2:895586/1‑150 (MQ=255)
                                                                                                                                      cgACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTg                 <  2:1530112/150‑1 (MQ=255)
                                                                                                                                        cgttcgATTAACGCTTTATCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCa               >  1:934880/6‑150 (MQ=255)
                                                                                                                                         cGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCAt              <  1:465373/150‑1 (MQ=255)
                                                                                                                                           cTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATAc            <  1:981562/150‑1 (MQ=255)
                                                                                                                                           cTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATAc            >  2:791236/1‑150 (MQ=255)
                                                                                                                                             gATTAACGCTTTATCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACAGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCt          >  1:9085/1‑150 (MQ=255)
                                                                                                                                             gATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCACGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCt          >  1:819261/1‑150 (MQ=255)
                                                                                                                                               ttAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCtgt        >  1:17616/1‑150 (MQ=255)
                                                                                                                                                  aCGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTggg     >  1:28820/1‑150 (MQ=255)
                                                                                                                                                  aCGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTggg     <  2:901729/150‑1 (MQ=255)
                                                                                                                                                    gATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGgcg   <  1:518723/150‑1 (MQ=255)
                                                                                                                                                     aTAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGgcgc  >  1:1218352/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGCGGTGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCAGCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGC  >  NC_000913/3975583‑3975881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑