Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	436,699	T→A	6.2%	P37P (CCT→CCA)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,699	0	T	A	6.2%	305.4 / 7.8	96	P37P (CCT→CCA)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base T (49/41);  new base A (2/4);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGTGCACGAT  >  NC_000913/436551‑436846                                                                                                                                                     |                                                                                                                                                     tCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTg                                                                                                                                                     <  1:767073/150‑1 (MQ=255) tCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTAGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTg                                                                                                                                                     <  1:478308/150‑1 (MQ=255)  cGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTgg                                                                                                                                                    <  1:99903/150‑1 (MQ=255)   gTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTggg                                                                                                                                                   <  2:1181263/150‑1 (MQ=255)   gTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTggg                                                                                                                                                   >  1:912651/1‑150 (MQ=255)   gTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTggg                                                                                                                                                   <  1:1398321/150‑1 (MQ=255)   gTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTggg                                                                                                                                                   <  2:519485/150‑1 (MQ=255)     gACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGAc                                                                                                                                                 >  1:258557/1‑150 (MQ=255)            aaCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGAT‑cgggc                                                                                                                                        <  1:596834/150‑3 (MQ=255)               cTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCg                                                                                                                                      >  2:966399/1‑150 (MQ=255)               cTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCg                                                                                                                                      >  1:578013/1‑150 (MQ=255)                 gTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCt                                                                                                                                    <  1:1444583/150‑1 (MQ=255)                 gTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCt                                                                                                                                    >  1:48640/1‑150 (MQ=255)                  ttACATTAGACTGGAATGGATATGACCATTTTGCCACGCCTTTAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTTATCCGTGGCATCTAGTTGCTGTCGGATTCGGAAGTGTATTAAG‑CCGATCGTCCCAGGAACGATCGGGCttaatc                                                                                                                                   <  1:786517/150‑6 (MQ=255)                  ttACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGATCGGGCttaatc                                                                                                                                   <  2:1229033/150‑6 (MQ=255)                   tACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTgg                                                                                                                                  >  1:1298666/1‑150 (MQ=255)                   tACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTgg                                                                                                                                  >  1:832652/1‑150 (MQ=255)                   tACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCCATCGTCCCAGGAACGATCGGGCttaatc                                                                                                                                   <  2:1100915/150‑6 (MQ=255)                    aCATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGc                                                                                                                                 <  2:438342/150‑1 (MQ=255)                    aCATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGc                                                                                                                                 >  1:1068192/1‑150 (MQ=255)                    aCATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGCCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGc                                                                                                                                 <  2:555209/150‑1 (MQ=255)                     cATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCa                                                                                                                                >  1:264716/1‑150 (MQ=255)                     cATTAGACTGGAAAGGATATGACCATTTTCCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCa                                                                                                                                >  2:58029/1‑150 (MQ=255)                       ttAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGc                                                                                                                              <  1:316768/150‑1 (MQ=255)                       ttAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGc                                                                                                                              <  1:1419438/150‑1 (MQ=255)                            cTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTc                                                                                                                         >  1:1398788/1‑150 (MQ=255)                             tGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTcc                                                                                                                        <  2:1340404/150‑1 (MQ=255)                              ggAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCg                                                                                                                       >  2:1000738/1‑150 (MQ=255)                               gAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGt                                                                                                                      >  2:255582/1‑150 (MQ=255)                                    gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTgg                                                                                                                 <  1:972844/150‑1 (MQ=255)                                        tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATc                                                                                                             >  1:237086/1‑150 (MQ=255)                                         gACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCt                                                                                                            <  1:616521/150‑1 (MQ=255)                                          aCCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCtg                                                                                                           <  1:189815/150‑1 (MQ=255)                                           ccATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCtga                                                                                                          <  1:1000738/150‑1 (MQ=255)                                           ccAATTTGCCACGCCATAAAGCTGTCGCGAAAAGTCGCCTGAACATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGAGCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGGGAATCCGTTCTGGTATCtga                                                                                                          <  2:1415145/150‑1 (MQ=255)                                                   ccACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                                                                                  <  2:832652/150‑1 (MQ=255)                                                    cACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                                                                                 <  1:396552/150‑1 (MQ=255)                                                        ccATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGcc                                                                                             >  2:774841/1‑150 (MQ=255)                                                         cATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGccc                                                                                            >  2:584729/1‑150 (MQ=255)                                                          aTAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                                                                           <  2:863437/150‑1 (MQ=255)                                                           tAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTg                                                                                          <  2:265668/150‑1 (MQ=255)                                                            aaaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTgg                                                                                         >  1:1127647/1‑150 (MQ=255)                                                              aGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCa                                                                                       <  1:20965/150‑1 (MQ=255)                                                                aTGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGc                                                                                     <  2:1398788/150‑1 (MQ=255)                                                                 tGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGct                                                                                    >  1:344033/1‑150 (MQ=255)                                                                  gTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctc                                                                                   >  1:855788/1‑150 (MQ=255)                                                                    cgcgAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCAGTCGGATTCGGAAGAGGATTAAGCCCGATCGTTCCTGGCACGAT‑GGGCTCGCTGGCAGCCATTCCGTACTGATATCTCAAGACATTTTTGCCCAGGCacatata                                                                                 >  1:222359/1‑144 (MQ=255)                                                                           aGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtg                                                                          >  1:533732/1‑150 (MQ=255)                                                                            gTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtgg                                                                         >  1:944209/1‑150 (MQ=255)                                                                            gTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtgg                                                                         <  1:594749/150‑1 (MQ=255)                                                                            gTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtgg                                                                         >  1:1371514/1‑150 (MQ=255)                                                                                 cTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGgtggtg                                                                    <  1:966399/150‑1 (MQ=255)                                                                                  tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAATCACGATCGTTCATGGGACGAT‑GGTATCGATGGCAGCGATTACGTTCTGGTATCTGATGACCTTTTTGCACTGGCAGCTCTACTCGCTGGTGGTGa                                                                   >  1:1229929/1‑150 (MQ=255)                                                                                  tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGa                                                                   <  1:1151687/150‑1 (MQ=255)                                                                                   gAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGCT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCACTGGCAGCTCTACTCGCTGGTGGTGAt                                                                  >  2:922427/1‑150 (MQ=255)                                                                                   gAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt                                                                  >  1:649173/1‑150 (MQ=255)                                                                                    aaGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATg                                                                 >  2:32900/1‑150 (MQ=255)                                                                                    aaGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATg                                                                 >  2:489830/1‑150 (MQ=255)                                                                                     aGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGc                                                                <  2:201574/150‑1 (MQ=255)                                                                                      gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                                                                               <  2:1393371/118‑1 (MQ=255)                                                                                      gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                                                                               >  1:1393371/1‑118 (MQ=255)                                                                                          aGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg                                                           >  1:1187294/1‑150 (MQ=255)                                                                                          aGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg                                                           >  1:1182070/1‑150 (MQ=255)                                                                                          aGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg                                                           >  1:973595/1‑150 (MQ=255)                                                                                           gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGGGGTGATGCTGGGGa                                                          <  2:1462686/150‑1 (MQ=255)                                                                                               tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGTCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATTCTGGGGATCTg                                                      <  2:258557/150‑1 (MQ=255)                                                                                               tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCGGCGATGCGGTGCTGGTAGATGATGCCGTTTTTGCCCTGGCCGGTCTACTCGGGGGTGGTGAGGCggggtacggg                                                      >  1:793170/1‑144 (MQ=255)                                                                                                  gTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTAt                                                   >  1:1088926/1‑150 (MQ=255)                                                                                                    ggCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCg                                                                                                                             >  2:151263/1‑74 (MQ=255)                                                                                                    ggCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCg                                                                                                                             <  1:151263/74‑1 (MQ=255)                                                                                                      cATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc                                                        >  2:225062/1‑141 (MQ=255)                                                                                                      cATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc                                                        <  1:225062/141‑1 (MQ=255)                                                                                                      cATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGc                                               >  1:1398134/1‑150 (MQ=255)                                                                                                       aTCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCg                                              <  1:58029/150‑1 (MQ=255)                                                                                                          tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCt                                           <  2:264716/150‑1 (MQ=255)                                                                                                              tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTc                                            >  2:969848/1‑145 (MQ=255)                                                                                                              tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTc                                            <  1:969848/145‑1 (MQ=255)                                                                                                               gCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCtt                                      <  1:388851/150‑1 (MQ=255)                                                                                                                cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCttt                                     <  1:499366/150‑1 (MQ=255)                                                                                                                cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCttt                                     >  2:722513/1‑150 (MQ=255)                                                                                                                    cGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGtca                                 >  2:16281/1‑150 (MQ=255)                                                                                                                    cGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGtca                                 >  1:679879/1‑150 (MQ=255)                                                                                                                     ggATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGtcat                                >  2:1219185/1‑150 (MQ=255)                                                                                                                             aaGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGc                        >  1:93206/1‑150 (MQ=255)                                                                                                                                  gATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAg                   <  2:93206/150‑1 (MQ=255)                                                                                                                                  gATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAg                   >  2:960507/1‑150 (MQ=255)                                                                                                                                   aTTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc                                                        >  2:1110790/1‑112 (MQ=255)                                                                                                                                   aTTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc                                                        <  1:1110790/112‑1 (MQ=255)                                                                                                                                    ttAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGATGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGAc                 >  2:74293/1‑150 (MQ=255)                                                                                                                                        gCCCGCTCGTTCCTGCGACCAT‑GCGCTCTATCGCAGCGATTCAGTTCTGGTATCTGATGACCTATTTGCCCTGGCAGCTCTACTCGATGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATAAAACGGAGAAAGACAtcg             >  2:880333/1‑148 (MQ=255)                                                                                                                                        agcccaTCGTCCCAGGAACGATCGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTATGACATCAAACGGCGAAAGACATg              >  2:1435409/6‑150 (MQ=255)                                                                                                                                          cccaTCGTCCCAGGAACGATCGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATggg            >  1:552660/4‑150 (MQ=255)                                                                                                                                           cgttCGTTCCTTGGACGAT‑TTGCTCGCTGGCAGCGATTCAGTTCTTGTTTCTGATGACCTTTTTGCCCTGGCAGGTCTACTCGCTGGTGTTGATGGTGTTGATATGTATCTGCGTCTATCTTTGTAATGAAACGGCTAAAGAAATTGgtg          <  2:222359/147‑1 (MQ=255)                                                                                                                                             aTCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGtgtg        <  2:715077/150‑1 (MQ=255)                                                                                                                                                gTTCCTGTGACGAT‑GGGCTATCTGCCAGCGATTCCGTTCTGGTATCTGAAGACCTTTTTGCCATGGAAGCTCTAATCGCTCGAGGTCAAGCTGGGGATCTGTATCCGCGTCTAAATTTGTCATCAAACAGCGAAAGACATGCCTGTGCAc     >  2:139623/1‑150 (MQ=255)                                                                                                                                                   ccTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGTGCACGAt  >  2:1103948/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGTGCACGAT  >  NC_000913/436551‑436846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑