Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 494,034 A→T 8.3% intergenic (+11/‑42) dnaX → / → ybaB DNA polymerase III subunit tau/putative nucleoid‑associated protein YbaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913494,0340AT 8.3% 290.0 / 11.2 95intergenic (+11/‑42)dnaX/ybaBDNA polymerase III subunit tau/putative nucleoid‑associated protein YbaB
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base T (5/3);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCG  >  NC_000913/493887‑494178
                                                                                                                                                   |                                                                                                                                                
cgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGc                                                                                                                                                <  2:584063/150‑1 (MQ=255)
  cTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCtt                                                                                                                                              <  2:529907/150‑1 (MQ=255)
  cTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCtt                                                                                                                                              <  1:861357/150‑1 (MQ=255)
    ggAGTGGCGTCAGGGGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTAc                                                                                                                                            >  1:1021663/1‑150 (MQ=255)
    ggAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTAc                                                                                                                                            <  1:1341021/150‑1 (MQ=255)
      aGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGt                                                                                                                                          <  2:79672/150‑1 (MQ=255)
        gggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGAGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCTGTGCTTACGATc                                                                                                                                        <  1:1443999/149‑1 (MQ=255)
         ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                       <  2:93377/150‑1 (MQ=255)
          gCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGt                                                                                                                                      <  2:1251298/150‑1 (MQ=255)
            gTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCa                                                                                                                                    <  2:323784/150‑1 (MQ=255)
               aGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGAACGTAAGCTGTGCTTACGATcaaatggg                                                                                                                                 <  1:1219150/150‑8 (MQ=255)
                       taCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTg                                                                                                                         >  2:381754/1‑150 (MQ=255)
                       taCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTg                                                                                                                         >  1:476834/1‑150 (MQ=255)
                       taCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTg                                                                                                                         >  1:192881/1‑150 (MQ=255)
                        aCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGa                                                                                                                        >  1:663921/1‑150 (MQ=255)
                          gaagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAtt                                                                                                                      <  2:773966/150‑1 (MQ=255)
                          gaagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAtt                                                                                                                      <  2:1057188/150‑1 (MQ=255)
                           aagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGTATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTg                                                                                                                     <  2:697299/150‑1 (MQ=255)
                             gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                   >  1:298008/1‑150 (MQ=255)
                               aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                 >  1:590722/1‑150 (MQ=255)
                                 aaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagagag                                                                                                               <  2:227554/150‑1 (MQ=255)
                                  aCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagagaga                                                                                                              <  1:1175659/150‑1 (MQ=255)
                                   cTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGaa                                                                                                             <  2:1489771/150‑1 (MQ=255)
                                         cAGGCGCGCGAGTCCATTATTCCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTAt                                                                                                       <  2:568948/150‑1 (MQ=255)
                                             cgcgcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGttc                                                                                                   >  2:8076/1‑149 (MQ=255)
                                              gcgcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTg                                                                                                  <  2:1355220/150‑1 (MQ=255)
                                                gcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGt                                                                                                <  2:403240/150‑1 (MQ=255)
                                                gcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGt                                                                                                <  1:1493835/150‑1 (MQ=255)
                                                gcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGt                                                                                                <  1:756278/150‑1 (MQ=255)
                                                      ccATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGc                                                                                          <  2:41112/150‑1 (MQ=255)
                                                      ccATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGc                                                                                          >  1:771495/1‑150 (MQ=255)
                                                        attattGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAgg                                                                                           >  1:524810/1‑147 (MQ=255)
                                                        attattGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAgg                                                                                           <  2:524810/147‑1 (MQ=255)
                                                            ttGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTg                                                                                    >  1:285069/1‑150 (MQ=255)
                                                             tGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTgg                                                                                   >  1:353938/1‑150 (MQ=255)
                                                              gCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTggg                                                                                  <  1:574883/150‑1 (MQ=255)
                                                              gCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTggg                                                                                  <  2:252236/150‑1 (MQ=255)
                                                               cGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGt                                                                                 <  2:1021663/150‑1 (MQ=255)
                                                                 gATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTaa                                                                               <  1:192330/150‑1 (MQ=255)
                                                                 gATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTaa                                                                               <  2:770392/150‑1 (MQ=255)
                                                                   taataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAcc                                                                             <  2:192881/150‑1 (MQ=255)
                                                                    aataataTGCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGCGAGAAACCTATGTTTGGTAAAGGCTGTCGGGGTAACCt                                                                            <  2:1531500/150‑1 (MQ=255)
                                                                     ataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtg                                                                           >  2:592561/1‑150 (MQ=255)
                                                                     ataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtg                                                                           >  1:931908/1‑150 (MQ=255)
                                                                      taataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtga                                                                          >  2:1321107/1‑150 (MQ=255)
                                                                      taataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtga                                                                          >  2:806529/1‑150 (MQ=255)
                                                                           ttCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaag                                                                     >  1:484210/1‑150 (MQ=255)
                                                                            tCAGACCGTGCGTCGGTTCTTCGATGCGGAGCTGGATGAATAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCTTCCTTATCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagc                                                                    <  1:173122/150‑1 (MQ=255)
                                                                            tCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagc                                                                    >  2:322526/1‑150 (MQ=255)
                                                                            tCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagc                                                                    <  1:1397939/150‑1 (MQ=255)
                                                                             cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                   >  1:1444719/1‑150 (MQ=255)
                                                                             cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                   >  2:1153740/1‑150 (MQ=255)
                                                                                 cccTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTGTGGGAAAGGCGGTCTGGGTAACCTGATGAAGCAAGcc                                                               >  2:1449909/1‑150 (MQ=255)
                                                                                       gTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagcag                                                         <  2:298008/150‑1 (MQ=255)
                                                                                         cGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGAt                                                       <  2:771495/150‑1 (MQ=255)
                                                                                          ggTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATg                                                      <  2:931467/150‑1 (MQ=255)
                                                                                           gTTCTTCGATGCGGAGCTGTATGAAGAAAGTATCCGCCCAATTTGATCGTAAGCACAGCTTACGTTCGTCATACTTAACGTGATTTAGAGTTTAACCTATGTTTGGTAATGGCGGTCTTGGTAACCTGATGAAGCAAGCCCAGCAGATGc                                                     <  2:254901/150‑1 (MQ=255)
                                                                                           gTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGc                                                     >  1:179302/1‑150 (MQ=255)
                                                                                            ttcttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                                                    >  2:397199/1‑150 (MQ=255)
                                                                                            ttcttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                                                    <  2:70970/150‑1 (MQ=255)
                                                                                            ttcttcGATGCGGAGCTGGATGAAGAAAGTAACCGACACATTTGATCGTAAGCACAGCTTACGTTCGTCATAATTAACGTGATAGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAACTGATGAAGCAAGCCAAGCAGATGCa                                                    >  2:183777/1‑150 (MQ=255)
                                                                                              cttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                  <  1:556326/150‑1 (MQ=255)
                                                                                                tcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGaa                                                >  1:719397/1‑150 (MQ=255)
                                                                                                  gATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGaaaa                                              >  2:1035726/1‑150 (MQ=255)
                                                                                                  gATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGaaaa                                              <  1:528027/150‑1 (MQ=255)
                                                                                                     gCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaat                                           <  1:1284650/150‑1 (MQ=255)
                                                                                                      cGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatg                                          >  1:366915/1‑150 (MQ=255)
                                                                                                      cGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatg                                          >  1:420889/1‑150 (MQ=255)
                                                                                                       ggAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAAAAACCTATGATTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgc                                         <  2:358719/150‑1 (MQ=255)
                                                                                                        gAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgca                                        >  2:555585/1‑150 (MQ=255)
                                                                                                                 gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgca                               <  2:476834/150‑1 (MQ=255)
                                                                                                                 gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgca                               >  2:651669/1‑150 (MQ=255)
                                                                                                                   agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCCAGCCAAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                             >  1:1275345/1‑150 (MQ=255)
                                                                                                                   agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                             >  1:639277/1‑150 (MQ=255)
                                                                                                                   agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                             >  1:347343/1‑150 (MQ=255)
                                                                                                                    gaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGAAGGa                            >  1:663381/1‑150 (MQ=255)
                                                                                                                    gaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGGCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGa                            <  2:375045/150‑1 (MQ=255)
                                                                                                                            ccGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcg                    >  2:1206168/1‑150 (MQ=255)
                                                                                                                             cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                   >  2:1252375/1‑150 (MQ=255)
                                                                                                                              gCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgcg                  >  1:1142378/1‑150 (MQ=255)
                                                                                                                               ccccATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCgcgc                 <  1:907246/150‑1 (MQ=255)
                                                                                                                                     acgaacGTAAGCTGTGCTTACGATCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg           >  1:666454/6‑150 (MQ=255)
                                                                                                                                      tGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGa          <  1:1127398/150‑1 (MQ=255)
                                                                                                                                      cgaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGa          >  1:896084/5‑150 (MQ=255)
                                                                                                                                      cgaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGa          >  1:89519/5‑150 (MQ=255)
                                                                                                                                      cgaacGTAAGCTGTGCTTACGATCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAaga                         >  2:742986/5‑135 (MQ=255)
                                                                                                                                      cgaacGTAAGCTGTGCTTACGATCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAaga                         <  1:742986/131‑1 (MQ=255)
                                                                                                                                       gATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGaa         <  2:1353845/150‑1 (MQ=255)
                                                                                                                                        ttCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAGCTATGTTTGGTAAAGGCGGTCTGGGTATCCTGATGAAGCAAGCCGAGCAGATGCAAGAAAAATTGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg        <  2:575955/149‑1 (MQ=255)
                                                                                                                                        aacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg        >  1:874985/3‑150 (MQ=255)
                                                                                                                                        aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg        >  1:1396627/1‑150 (MQ=255)
                                                                                                                                        aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg        <  1:592561/150‑1 (MQ=255)
                                                                                                                                            tAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCAc    >  1:846900/1‑150 (MQ=255)
                                                                                                                                              aGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCg  >  2:87291/1‑150 (MQ=255)
                                                                                                                                              aGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCg  >  1:1010063/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCG  >  NC_000913/493887‑494178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑