| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,160,449 | C→A | 6.4% | G368V (GGC→GTC) | fhuE ← | ferric coprogen/ferric rhodotorulic acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,160,449 | 0 | C | A | 6.4% | 207.2 / 3.8 | 62 | G368V (GGC→GTC) | fhuE | ferric coprogen/ferric rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (26/32); new base A (2/2); total (28/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
TGGCCCATGAACTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAG > NC_000913/1160300‑1160596 | tGGCCCATGAACTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTgccgc > 1:602566/1‑150 (MQ=255) ccATGAACTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGAc > 2:805840/1‑150 (MQ=255) cATGAACTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACa > 2:971867/1‑150 (MQ=255) tGAACTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATa < 2:20354/150‑1 (MQ=255) cTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCa < 1:1061784/150‑1 (MQ=255) gATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTc < 1:383648/150‑1 (MQ=255) aTTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTcc < 2:601893/150‑1 (MQ=255) gtttgCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTAc > 1:584276/1‑150 (MQ=255) gtttgCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTAc < 1:1408046/150‑1 (MQ=255) gCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTa > 1:110801/1‑150 (MQ=255) gCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTa < 1:478427/150‑1 (MQ=255) tAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGc < 1:946059/150‑1 (MQ=255) aCTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGccc > 2:921046/1‑150 (MQ=255) gCCACCAAACATTAGATTGTGCTGACGCCCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATATTCAAAGCCAGGTCCATAATTACTGTATGGCCCAAc > 2:1072882/1‑150 (MQ=255) ccaccaAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAAcc < 1:225292/150‑1 (MQ=255) ccaccaAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAAcc > 2:956521/1‑150 (MQ=255) caccaAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCa > 1:74411/1‑150 (MQ=255) accaAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCTAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAg > 2:107903/1‑150 (MQ=255) accaAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAg > 1:998699/1‑150 (MQ=255) aaaCATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCAt < 1:355803/150‑1 (MQ=255) aCATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATAc < 2:110801/150‑1 (MQ=255) ttGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCt < 1:805840/150‑1 (MQ=255) tgtgCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGcc > 1:1446017/1‑129 (MQ=255) tgtgCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGcc < 2:1446017/129‑1 (MQ=255) gtgCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCttt < 1:614631/150‑1 (MQ=255) gCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGt < 1:525573/150‑1 (MQ=255) gCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAACCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGt < 1:528801/150‑1 (MQ=255) tGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGttt < 2:1406109/150‑1 (MQ=255) tGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGttt < 2:745641/150‑1 (MQ=255) cgacCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACa < 1:1233415/150‑1 (MQ=255) cAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAgg < 1:1140603/150‑1 (MQ=255) cAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTc > 1:646164/1‑95 (MQ=255) cAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTc < 2:646164/95‑1 (MQ=255) aTTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGa > 2:1383369/1‑150 (MQ=255) aTAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGacata < 2:602566/150‑1 (MQ=255) aTAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCC‑ACGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGacatac > 1:1227750/1‑150 (MQ=255) aTAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCC‑ACGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGacatac > 1:1233084/1‑150 (MQ=255) tAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGacatac > 2:1494392/1‑150 (MQ=255) aCCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATAcatcat > 2:910114/1‑150 (MQ=255) ccGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCAtt > 2:1386268/1‑150 (MQ=255) ccGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCAtt < 1:124612/150‑1 (MQ=255) aGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGacataca < 2:232765/140‑1 (MQ=255) aGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGacataca > 1:232765/1‑140 (MQ=255) gCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGa > 1:108067/1‑133 (MQ=255) gCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGa < 2:108067/133‑1 (MQ=255) cGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCCACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCATTGTTTACATAGGCATCGACATACATAATTTTGCTGt > 2:698095/1‑150 (MQ=255) cAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTG‑TTACATAGGCATCGACATACATCATTTTGCTGTCAAAt < 1:971867/150‑1 (MQ=255) ataTCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAAt < 1:1494392/148‑1 (MQ=255) tCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCg < 2:584276/150‑1 (MQ=255) cAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAgt > 2:1336549/1‑150 (MQ=255) ttACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAgtgggtg < 2:1057261/150‑1 (MQ=255) aCGTTTGCCACTGTTCCAACCGGTGCC‑ACGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCa < 2:1233084/150‑1 (MQ=255) aCGTTTGCCACTGTTCCAACCGGTGCC‑ACGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCa < 2:1227750/150‑1 (MQ=255) ttGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTc < 2:998699/150‑1 (MQ=255) ttGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTc < 1:1518051/150‑1 (MQ=255) gCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAg > 2:345876/1‑150 (MQ=255) ccACTGTTCCAACCGGTGCCACCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCa < 2:1430635/148‑1 (MQ=255) ccACTGTTCCAACCGGTGCCACCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCa > 1:1430635/1‑148 (MQ=255) cACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAgtg > 2:208510/1‑150 (MQ=255) gTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGc > 2:612252/1‑150 (MQ=255) cccaCCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGCTTg > 2:144841/4‑150 (MQ=255) cAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGCTTGc > 2:750351/1‑150 (MQ=255) cAACCGGTGACGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGCTTGc < 2:971751/150‑1 (MQ=255) cgccgACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCTTACCATCCGCTTTGTTTACATAGGCATCTACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGCTATAAg < 2:1269535/150‑1 (MQ=255) | TGGCCCATGAACTGAAGTAACGATTGTTTTGTTTGCTGTAACTGCCACCAAACATTAGATTGTGCTGACGACCAAACAATTCATAACTACCGTCAGCGAACAAATCCAGCGCATCAACTTTACGTTTGCCACTGTTCCAACCGGTGCCGCCGACATAATCAAAGCCAGGTCCATAATTACTGTATGGCCCAACCAGCATACCATCCGCTTTGTTTACATAGGCATCGACATACATCATTTTGCTGTCAAATTCGACTTCAGAGTGGGTGGCATTCAGTGTCGCTTGCCAGGTATCAG > NC_000913/1160300‑1160596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |