Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,408,546 A→T 5.4% H166L (CAC→CTC)  zntB → Zn(2(+)):H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,5460AT 5.4% 299.7 / 5.0 93H166L (CAC→CTC) zntBZn(2(+)):H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (41/47);  new base T (3/2);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

GTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACG  >  NC_000913/1408398‑1408693
                                                                                                                                                    |                                                                                                                                                   
gTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCAc                                                                                                                                                    <  1:1043362/150‑1 (MQ=255)
 tttCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGTCTGGTGGTTGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGGTGCACg                                                                                                                                                   <  2:269042/150‑1 (MQ=255)
    cGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATa                                                                                                                                                >  1:1158633/1‑150 (MQ=255)
     gACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaa                                                                                                                                               >  1:27829/1‑150 (MQ=255)
      aCCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGG‑AGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaat                                                                                                                                             <  2:685232/150‑2 (MQ=255)
       cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                             <  1:587796/150‑1 (MQ=255)
       cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                             <  1:931838/150‑1 (MQ=255)
       cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                             >  2:292198/1‑150 (MQ=255)
         cGACAACGCAACGGGCGGGCGCTGGACGCTGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGTTTGCGGGTGAGGTGTGGTGGATGTGTGCGATGCGTTGAGGGATCATTCCCGTGATTTTATCGAGCAGCTGCACGATAAAAtt                                                                                                                                           <  1:125241/150‑1 (MQ=255)
         cGACAACGCAAAGTGATGGCGCTTGAAGATGTGGTGAGCGATCTGGAAGAGGCAACGGGTACGACCGATTTCGGGGGATGTCTGGTGGATGTGTTCGATGCGTTGACCTATCATTACATTGAATTTATCGAGCAGCTTCACGATAAAAtt                                                                                                                                           <  1:130698/150‑1 (MQ=255)
            cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATc                                                                                                                                        <  2:869151/150‑1 (MQ=255)
             aaCGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGGCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGGTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCg                                                                                                                                       <  2:1339242/150‑1 (MQ=255)
              aCGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGa                                                                                                                                      >  2:646301/1‑150 (MQ=255)
               cGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAc                                                                                                                                     <  2:375072/150‑1 (MQ=255)
               cGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAc                                                                                                                                     >  1:286493/1‑150 (MQ=255)
                 cAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCg                                                                                                                                       <  2:1339181/146‑1 (MQ=255)
                 cAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCg                                                                                                                                       >  1:1339181/1‑146 (MQ=255)
                 cAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCt                                                                                                                                   >  2:500218/1‑150 (MQ=255)
                 cAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCt                                                                                                                                   >  1:1492028/1‑150 (MQ=255)
                  aaaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCtt                                                                                                                                  >  2:996506/1‑150 (MQ=255)
                     gTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                                         >  1:802350/1‑135 (MQ=255)
                     gTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                                         <  2:802350/140‑6 (MQ=255)
                         tGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATa                                                                                                                           <  2:1528237/150‑1 (MQ=255)
                            cgcTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAtc                                                                                                                        <  1:911103/150‑1 (MQ=255)
                                  aCGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTg                                                                                                                  <  2:126567/150‑1 (MQ=255)
                                    gATGTGGTGTGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTTGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTCCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGAt                                                                                                                <  2:432775/150‑1 (MQ=255)
                                     aTGTGGTGAGCGATCTGGAAGAGGGGACGGGTCCGACGGATTTCGGGGGATGGGTGGTGGATGTGTGCGATGCGTTGACCTATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATc                                                                                                               <  2:803598/150‑1 (MQ=255)
                                     aTGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATc                                                                                                               >  1:1122468/1‑150 (MQ=255)
                                         ggtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGGAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCa                                                                                                           <  2:1112491/150‑1 (MQ=255)
                                         ggtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCa                                                                                                           >  2:1223633/1‑150 (MQ=255)
                                          gtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCaa                                                                                                          <  1:616938/150‑1 (MQ=255)
                                           tgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCaaa                                                                                                         <  1:605981/150‑1 (MQ=255)
                                            gAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAt                                                                                                        <  2:903227/150‑1 (MQ=255)
                                             aGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTTGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAtt                                                                                                       <  1:95340/150‑1 (MQ=255)
                                                gATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCa                                                                                                    >  1:1350852/1‑150 (MQ=255)
                                                   gtGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAcct                                                                                                 <  2:586262/149‑2 (MQ=255)
                                                    tGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcgc                                                                                                >  1:877312/1‑150 (MQ=255)
                                                     ggAAGAGGGCACCGGTCCGACCCATTCCGGGCGATGCCTGGTGGATGTGTGCGAAGCGATGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTAAAGATAATCTACTTGATCAGCAAATTCCACcgcg                                                                                               >  2:652939/1‑150 (MQ=255)
                                                        agagGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTgg                                                                                            >  1:1505303/1‑150 (MQ=255)
                                                             gCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGATTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTcc                                                                                       <  2:1275693/150‑1 (MQ=255)
                                                             gCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTcc                                                                                       <  2:1280946/150‑1 (MQ=255)
                                                              cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                                      >  1:347100/1‑150 (MQ=255)
                                                              cACGGGTCAGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                                      >  2:1318714/1‑150 (MQ=255)
                                                                cGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTgg                                                                                    <  2:1492028/150‑1 (MQ=255)
                                                                 gggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                                   <  1:1407484/150‑1 (MQ=255)
                                                                 gggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                                   <  2:1158633/150‑1 (MQ=255)
                                                                  ggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGAACAGCAAATTCCACCGCGTGGATTCCTGGct                                                                                  >  1:1345048/1‑150 (MQ=255)
                                                                        accgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgctg                                                                            <  1:64012/150‑1 (MQ=255)
                                                                        accgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgctg                                                                            >  2:1401994/1‑150 (MQ=255)
                                                                          cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                                      <  1:729640/138‑1 (MQ=255)
                                                                          cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                                      >  2:729640/1‑138 (MQ=255)
                                                                           gaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTgcgc                                                                         <  2:27829/150‑1 (MQ=255)
                                                                               gCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAAc                                                                     >  2:722106/1‑150 (MQ=255)
                                                                                cGGGGGATAGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACa                                                                    <  2:442475/150‑1 (MQ=255)
                                                                                cGGGGGACGGCTCGGGTATGTGTGCGATTCTTTTACCGATCATTCCAGTGAATTTATCGATCAGCTGCACGATAAAATTATCGACCTTGAAGCTAATCTCCTTGATGAGCAAATTCCACCTCGTGGATTCCTGGCTCTGCTGCGCAAACa                                                                    <  2:1012600/150‑1 (MQ=255)
                                                                                 gggggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaa                                                                   <  2:139324/150‑1 (MQ=255)
                                                                                  ggggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaat                                                                  >  2:835716/1‑150 (MQ=255)
                                                                                      aTGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaat                                                              <  1:424342/150‑1 (MQ=255)
                                                                                       tGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaatt                                                             >  2:1227549/1‑150 (MQ=255)
                                                                                         tcTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgt                                                           <  1:190132/149‑1 (MQ=255)
                                                                                                 aTGTGTGCGAAGCCTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCAAGATAAAATTATCGACCTTGAAGATAATCTACTTGATCAGCAAATTCCACCGCGTGGAATCGAGGCTCTGCTGCGCAAACAATTAATTGTGATGCGta                                                   >  2:1412386/1‑149 (MQ=255)
                                                                                                   gtgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGc                                                 <  1:500218/150‑1 (MQ=255)
                                                                                                    tgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCt                                                >  2:1069374/1‑150 (MQ=255)
                                                                                                     gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCta                                               >  2:1110596/1‑150 (MQ=255)
                                                                                                       gCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtata                                             >  1:1450125/1‑150 (MQ=255)
                                                                                                         gATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATg                                           >  1:1171671/1‑150 (MQ=255)
                                                                                                         gATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATg                                           <  1:495365/150‑1 (MQ=255)
                                                                                                          aTGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAAATGTGATGCGTCGCTATATgg                                          >  2:741930/1‑150 (MQ=255)
                                                                                                             cGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGa                                                         <  1:413240/132‑1 (MQ=255)
                                                                                                             cGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGa                                                         >  2:413240/1‑132 (MQ=255)
                                                                                                             cGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCAc                                       >  1:415074/1‑150 (MQ=255)
                                                                                                              gTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTGATTGTGATGCGTCGCTATATGGCaac                                      <  2:1505303/150‑3 (MQ=255)
                                                                                                                 gACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCa                                   >  2:69472/1‑150 (MQ=255)
                                                                                                                     gATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                               >  1:1322643/1‑150 (MQ=255)
                                                                                                                      aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCATCAAATTCCACCTCGTGGTTTCCTGTCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCAGCGCAACGTg                              <  2:1318367/150‑1 (MQ=255)
                                                                                                                      aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTg                              <  2:415074/150‑1 (MQ=255)
                                                                                                                        cATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGAt                            >  1:730631/1‑150 (MQ=255)
                                                                                                                        cATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGAt                            >  2:1006525/1‑150 (MQ=255)
                                                                                                                             cgataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTa                       >  1:1238338/8‑150 (MQ=255)
                                                                                                                               ataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATg                     >  1:416852/6‑150 (MQ=255)
                                                                                                                                tGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGc                    <  1:305424/150‑1 (MQ=255)
                                                                                                                                 gAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt                   <  1:1318714/150‑1 (MQ=255)
                                                                                                                                   aTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCg                 <  2:1004499/150‑1 (MQ=255)
                                                                                                                                          gAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGcc          <  1:1006525/150‑1 (MQ=255)
                                                                                                                                          gAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGcc          >  1:189168/1‑150 (MQ=255)
                                                                                                                                          gAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCACAACGTGATGTTTATGCTCGTCTTGcc          >  2:928329/1‑150 (MQ=255)
                                                                                                                                           agcagcTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCa         >  2:864350/1‑150 (MQ=255)
                                                                                                                                              agcTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTg      <  1:1401994/150‑1 (MQ=255)
                                                                                                                                                  gCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCaa                                  >  1:1354185/1‑118 (MQ=255)
                                                                                                                                                  gCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCaa                                  <  2:1354185/118‑1 (MQ=255)
                                                                                                                                                  gCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACg  <  2:1450125/150‑1 (MQ=255)
                                                                                                                                                  gCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACg  >  1:1003187/1‑150 (MQ=255)
                                                                                                                                                  gCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACg  >  1:228278/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACG  >  NC_000913/1408398‑1408693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑