Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,966 T→A 5.1% intergenic (‑39/+18) ralR ← / ← recT Rac prophage; endodeoxyribonuclease toxin RalR/Rac prophage; recombinase, DNA renaturation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,9660TA 5.1% 349.6 / 4.4 108intergenic (‑39/+18)ralR/recTRac prophage; endodeoxyribonuclease toxin RalR/Rac prophage; recombinase, DNA renaturation
Reads supporting (aligned to +/- strand):  ref base T (44/58);  new base A (2/4);  total (46/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTT  >  NC_000913/1413823‑1414115
                                                                                                                                               |                                                                                                                                                     
ggATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACAcc                                                                                                                                                 <  1:1437613/150‑1 (MQ=255)
 gATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCg                                                                                                                                                >  1:1369971/1‑150 (MQ=255)
  aTTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGc                                                                                                                                               <  2:421194/150‑1 (MQ=255)
   ttCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGcc                                                                                                                                              <  1:696578/150‑1 (MQ=255)
   ttCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGcc                                                                                                                                              >  1:1410729/1‑150 (MQ=255)
      gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATTATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                                                                           >  2:1429021/1‑150 (MQ=255)
      gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                           <  2:238846/150‑3 (MQ=255)
      gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                                                                           >  1:1162065/1‑150 (MQ=255)
        aTCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCt                                                                                                                                         <  2:1328132/150‑1 (MQ=255)
          ccGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaacttga                                                                                                                                       <  1:1422688/150‑7 (MQ=255)
          ccGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                                                                       <  1:513346/150‑1 (MQ=255)
          ccGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                                                                       <  1:1485640/150‑1 (MQ=255)
           cGTTACCCTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTGAAGTTGGCGGTGCATTACCCCGCCAGGCTGaa                                                                                                                                      >  2:189045/1‑150 (MQ=255)
            gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAat                                                                                                                                     <  1:1230177/150‑1 (MQ=255)
            gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAat                                                                                                                                     <  1:322953/150‑1 (MQ=255)
             ttACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatt                                                                                                                                    <  1:846005/150‑1 (MQ=255)
             ttACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatt                                                                                                                                    <  2:190890/150‑1 (MQ=255)
               acacTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAattat                                                                                                                                  >  2:391709/1‑150 (MQ=255)
                cacTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAattatt                                                                                                                                 >  1:485893/1‑150 (MQ=255)
                  cTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                               >  1:446325/1‑150 (MQ=255)
                  cTTCGCTCGGTAATATCCTGAAATGGCTATCACCGTTACTGATGGACAACCACAAAATGGACATGGATTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                               >  2:778301/1‑150 (MQ=255)
                  cTTCGCTCGGTAATATCCTGAAATGCCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                               <  1:1174412/150‑1 (MQ=255)
                     cGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCAACTTGAaatatttttta                                                                                                                            <  1:1116387/150‑6 (MQ=38)
                       cTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGa                                                                                                                          <  2:106985/150‑1 (MQ=255)
                       cTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGa                                                                                                                          >  2:813340/1‑150 (MQ=255)
                                atCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 <  1:467031/150‑1 (MQ=255)
                                 tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAtt                                                                                                                >  2:101465/1‑150 (MQ=255)
                                  ccTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa                                                                                                               >  1:290942/1‑150 (MQ=255)
                                  ccTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa                                                                                                               >  1:597118/1‑150 (MQ=255)
                                     gAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                            <  1:91045/150‑1 (MQ=255)
                                       aaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTGTTCCTCTGAATTATCGATTACACTg                                                                                                          <  1:346838/150‑1 (MQ=255)
                                       aaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTg                                                                                                          <  1:177579/150‑1 (MQ=255)
                                       aaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTg                                                                                                          <  2:1470483/150‑1 (MQ=255)
                                            cTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTc                                                                                                     >  1:245254/1‑150 (MQ=255)
                                                 aCCGTTACTGATGGACAACCACAAAATGGACATTGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCgg                                                                                                <  2:165971/150‑1 (MQ=255)
                                                 aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCgg                                                                                                >  2:1518004/1‑150 (MQ=255)
                                                  ccGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGt                                                                                               <  1:1444507/150‑1 (MQ=255)
                                                   cGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                                                                              <  2:42054/150‑1 (MQ=255)
                                                      tACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAAt                                                                                           <  2:382603/150‑1 (MQ=255)
                                                      tACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACAC‑‑CCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATAc                                                                                         <  2:3782/150‑1 (MQ=255)
                                                       aCTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                          <  2:53675/150‑1 (MQ=255)
                                                          gATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACag                                                                                       <  2:735468/150‑1 (MQ=255)
                                                          gATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGATGAATTATTCGTATGATTTATCAATTAAAATGTATGCCCCGGTAAATACac                                                                                       >  2:1425293/1‑149 (MQ=255)
                                                            ttgACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACagag                                                                                     <  2:1449137/148‑1 (MQ=255)
                                                             ggACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAgg                                                                                    <  2:1410729/150‑1 (MQ=255)
                                                               aCAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGaa                                                                                  >  1:1331233/1‑150 (MQ=255)
                                                                  aCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCACTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCt                                                                               >  2:1363321/1‑150 (MQ=255)
                                                                   ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                                                                              >  1:596708/1‑150 (MQ=255)
                                                                    cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGc                                                                             >  2:396296/1‑150 (MQ=255)
                                                                    cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGc                                                                             <  2:735965/150‑1 (MQ=255)
                                                                    cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGc                                                                             >  1:453672/1‑150 (MQ=255)
                                                                     acaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCa                                                                            <  1:226781/150‑1 (MQ=255)
                                                                     acaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCa                                                                            >  1:834804/1‑150 (MQ=255)
                                                                      caAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                                                                           <  2:963078/150‑1 (MQ=255)
                                                                       aaaaTTGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAgg                                                                          <  2:1514676/150‑1 (MQ=255)
                                                                       aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAgg                                                                          >  1:1191513/1‑150 (MQ=255)
                                                                       aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAgg                                                                          >  1:1185953/1‑150 (MQ=255)
                                                                             gACATCGTCTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTAAATACAGAGGAATCTGCAGGATCGAt                                                                    <  2:286284/150‑1 (MQ=255)
                                                                               cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTg                                                                  <  1:1030802/150‑1 (MQ=255)
                                                                                 tGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa                                                                                                                  >  1:327642/1‑100 (MQ=255)
                                                                                   gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg                                                              >  1:390582/1‑150 (MQ=255)
                                                                                   gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg                                                              <  2:261935/150‑1 (MQ=255)
                                                                                    tttAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt                                                             <  2:834804/150‑1 (MQ=255)
                                                                                     ttAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTg                                                            >  1:1452364/1‑150 (MQ=255)
                                                                                      tAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTgg                                                           <  1:1451920/150‑1 (MQ=255)
                                                                                        aCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGtt                                                         >  2:885821/1‑150 (MQ=255)
                                                                                          aTTGTCATATCTCATTATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTACTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc                                                       <  1:1429021/150‑1 (MQ=255)
                                                                                           ttGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCt                                                      <  2:1191513/150‑1 (MQ=255)
                                                                                           ttGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCt                                                      <  2:1185953/150‑1 (MQ=255)
                                                                                               cATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTc                                                  >  1:15887/1‑150 (MQ=255)
                                                                                                 tatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCAt                                                <  1:1472937/150‑1 (MQ=255)
                                                                                                    ctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCa                                             <  1:1092351/150‑1 (MQ=255)
                                                                                                      cATAATTTTTCTCATAAAAAATAATTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCAtt                                           >  2:183455/1‑150 (MQ=255)
                                                                                                       aTAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTg                                          >  2:709770/1‑150 (MQ=255)
                                                                                                        tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGa                                         <  1:778301/150‑1 (MQ=255)
                                                                                                           tttttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATAc                                      <  2:1444211/150‑1 (MQ=255)
                                                                                                           tttttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATAc                                      <  1:692821/150‑1 (MQ=255)
                                                                                                                  caaaaaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAAGACAGAGGAAACTGCAGGATCGATTGTCAGTGGATCCTTTTCATCCATTGATACTGCACGc                               >  1:147681/4‑150 (MQ=255)
                                                                                                                     aaaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATACATTGATACTGCACGCTgg                            <  1:966886/150‑1 (MQ=255)
                                                                                                                     aaaaaaTATTTCAAGTTGACGGTGCAATACACAGCCACGCTGAATTATTCCTCTCAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                            >  2:85874/1‑150 (MQ=255)
                                                                                                                        aaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAtc                         <  2:269617/150‑1 (MQ=255)
                                                                                                                            atTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCaa                     <  2:596708/150‑1 (MQ=255)
                                                                                                                              ttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAtt                   <  2:15887/150‑1 (MQ=255)
                                                                                                                              ttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAtt                   <  1:391709/150‑1 (MQ=255)
                                                                                                                               tCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTg                  >  1:1406380/1‑150 (MQ=255)
                                                                                                                                cAAGTTGGCTGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTAACCGGTTAATACAGAGGAATCTGCTGGATCGATTGTCAGTGGTTCCTTTGCATCCATTGATACTGCACGCTGGATCTCAATTGa                 >  2:593043/1‑150 (MQ=255)
                                                                                                                                  aGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa               <  1:885821/150‑1 (MQ=255)
                                                                                                                                  aGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCACTGATACTGCACGCTGGATCTCAATTGATa               <  2:1331233/150‑1 (MQ=255)
                                                                                                                                   cctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTACACGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTACTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATAc              >  2:714725/3‑150 (MQ=255)
                                                                                                                                     tGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACgg            >  2:1470339/1‑150 (MQ=255)
                                                                                                                                      ggCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCa                                  >  1:1029597/1‑127 (MQ=255)
                                                                                                                                      ggCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCa                                  <  2:1029597/127‑1 (MQ=255)
                                                                                                                                         ggTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                          >  1:1400440/1‑68 (MQ=255)
                                                                                                                                         ggTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                          <  2:1400440/68‑1 (MQ=255)
                                                                                                                                           tGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAat      >  1:913464/1‑150 (MQ=255)
                                                                                                                                            gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAata     >  2:1226587/1‑150 (MQ=255)
                                                                                                                                              aTTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAtt   <  1:977574/150‑1 (MQ=255)
                                                                                                                                              aTTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAtt   <  2:1366575/150‑1 (MQ=255)
                                                                                                                                               ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAttt  <  2:485893/150‑1 (MQ=255)
                                                                                                                                               ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAttt  <  2:236112/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTT  >  NC_000913/1413823‑1414115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑