Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,499,365 A→T 8.3% Q143L (CAG→CTG)  rimL → ribosomal‑protein‑L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,499,3650AT 8.3% 253.5 / 10.9 84Q143L (CAG→CTG) rimLribosomal‑protein‑L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (41/36);  new base T (4/3);  total (45/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

CGCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCC  >  NC_000913/1499216‑1499513
                                                                                                                                                     |                                                                                                                                                    
cGCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCa                                                                                                                                                      <  1:614401/150‑1 (MQ=255)
cGCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCa                                                                                                                                                      <  1:19815/150‑1 (MQ=255)
    gAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTc                                                                                                                                                  <  1:1530419/150‑1 (MQ=255)
            cTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                                                                          <  1:1313774/150‑1 (MQ=255)
             tACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGTGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                                                                         <  1:437494/150‑2 (MQ=255)
              aCTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCa                                                                                                                                        >  1:1059091/1‑150 (MQ=255)
               ctggctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggat                                                                                                                                       <  1:72079/150‑4 (MQ=255)
                 ggctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATg                                                                                                                                     >  2:1297400/1‑150 (MQ=255)
                   ctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGt                                                                                                                                   >  1:1138032/1‑150 (MQ=255)
                     ggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGttt                                                                                                                                 <  1:747557/150‑1 (MQ=255)
                     ggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGttt                                                                                                                                 >  1:841074/1‑150 (MQ=255)
                      gACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                                                                                >  1:1429474/1‑150 (MQ=255)
                      gACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAAACAGGTCGCTTTGCGCAATGGtttt                                                                                                                                >  1:1345830/1‑150 (MQ=255)
                       aCGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                                                                               <  1:954439/150‑1 (MQ=255)
                          actctcATCACGGGCAGGGGATCATTTCTCAGGCGCGGCAGGCATTGATTCATCATTACGCCCAGTCTGCTGAACTTAGACGCTGCGTCATCAAATGGCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATcc                                                                                                                            <  2:573893/148‑1 (MQ=255)
                          aaTCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCAAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATcc                                                                                                                            >  2:423625/1‑150 (MQ=255)
                           aTCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCt                                                                                                                           >  1:547668/1‑150 (MQ=255)
                            tctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCtt                                                                                                                          <  2:365853/150‑1 (MQ=255)
                            tctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCtt                                                                                                                          >  2:45105/1‑150 (MQ=255)
                             ctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                                                                         >  2:1152295/1‑150 (MQ=255)
                                atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                      <  2:1494588/150‑1 (MQ=255)
                                    ggggcaggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTg                                                                                                                  >  2:325963/1‑150 (MQ=255)
                                     gggcaggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGc                                                                                                                 >  2:1023001/1‑150 (MQ=255)
                                      ggcaggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                                <  1:323893/150‑1 (MQ=255)
                                          ggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGaa                                                                                                            >  1:32370/1‑150 (MQ=255)
                                          ggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGaa                                                                                                            >  1:1293375/1‑150 (MQ=255)
                                              aTCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAg                                                                                                        >  2:1321140/1‑150 (MQ=255)
                                              aTCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAg                                                                                                        <  2:1059091/150‑1 (MQ=255)
                                                cATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGTGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGc                                                                                                      <  1:1130653/150‑1 (MQ=255)
                                                cATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGc                                                                                                      <  2:680630/150‑1 (MQ=255)
                                                 aTTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCt                                                                                                     >  2:253269/1‑150 (MQ=255)
                                                   ttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCTAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGa                                                                                                   <  2:1405566/150‑1 (MQ=255)
                                                     ctcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGt                                                                                                 >  2:324047/1‑150 (MQ=255)
                                                        aGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTcc                                                                                              >  2:312719/1‑150 (MQ=255)
                                                         ggCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCt                                                                                             <  1:835283/150‑1 (MQ=255)
                                                             cTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAat                                                                                         >  1:1459367/1‑150 (MQ=255)
                                                              tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatg                                                                                        <  2:23074/150‑1 (MQ=255)
                                                              tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatg                                                                                        >  2:499613/1‑150 (MQ=255)
                                                                 aGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                                                                     <  1:1321140/150‑1 (MQ=255)
                                                                  ggCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGc                                                                                    >  2:829897/1‑150 (MQ=255)
                                                                     aTTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTa                                                                                 <  1:490332/150‑1 (MQ=255)
                                                                        gATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatga                                                                              <  1:1206895/150‑1 (MQ=255)
                                                                            catcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgat                                                                          >  2:1208094/1‑150 (MQ=255)
                                                                               catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                                                                       <  2:547668/150‑1 (MQ=255)
                                                                                  tACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                                                                    <  2:1030733/150‑1 (MQ=255)
                                                                                  tACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                                                                    <  1:325963/150‑1 (MQ=255)
                                                                                    cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt                                                                  >  2:37703/1‑150 (MQ=255)
                                                                                    cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGGTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt                                                                  >  2:725104/1‑150 (MQ=255)
                                                                                    cGCCCAGTCTGGTGAACTTACACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt                                                                  >  1:407910/1‑150 (MQ=255)
                                                                                     gCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTta                                                                 >  1:403027/1‑150 (MQ=255)
                                                                                                tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                      >  1:1451715/1‑150 (MQ=255)
                                                                                                 gAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtatt                                                     <  2:1429474/150‑1 (MQ=255)
                                                                                                   aCTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtattat                                                   <  1:1297400/150‑1 (MQ=255)
                                                                                                   aCTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGG‑TTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATc                                                  <  2:33739/150‑1 (MQ=255)
                                                                                                    cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATc                                                  >  2:257062/1‑150 (MQ=255)
                                                                                                     ttAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCGTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCg                                                 <  1:499613/150‑1 (MQ=255)
                                                                                                      tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGa                                                >  1:760070/1‑150 (MQ=255)
                                                                                                           gCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcac                                           <  1:45105/150‑1 (MQ=255)
                                                                                                           gCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcac                                           <  1:458577/150‑1 (MQ=255)
                                                                                                                 tGATCAATTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATGCTTGAAGGTTGCCTGAAACAGGCTGAGTTGCTGCATGATGCCTTTGATGATGTGCACTTATACGCGCGTAGTTTCGCTTCACAATCAc                                     >  2:1244714/1‑150 (MQ=255)
                                                                                                                 tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAAc                                     >  1:1063341/1‑150 (MQ=255)
                                                                                                                 tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAAc                                     >  1:105322/1‑150 (MQ=255)
                                                                                                                  gATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAAcc                                    <  1:583982/150‑1 (MQ=255)
                                                                                                                         tGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAg                             <  2:841074/150‑1 (MQ=255)
                                                                                                                          gTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgc                            >  2:139038/1‑150 (MQ=255)
                                                                                                                          gTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgc                            <  2:309497/150‑1 (MQ=255)
                                                                                                                            cGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgcgg                          <  1:1208094/150‑1 (MQ=255)
                                                                                                                             gggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgcggc                         >  2:851819/1‑150 (MQ=255)
                                                                                                                              ggTGTACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACACTGCCAgcggcg                        <  2:1459367/150‑1 (MQ=255)
                                                                                                                              ggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgcggcg                        <  1:37703/150‑1 (MQ=255)
                                                                                                                              ggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgcggcg                        <  1:829897/150‑1 (MQ=255)
                                                                                                                                 ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCGTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTc                     >  2:1346559/1‑150 (MQ=255)
                                                                                                                                  gACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGTCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCACGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcg                    >  1:450434/1‑150 (MQ=255)
                                                                                                                                  gACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcg                    >  1:768626/1‑150 (MQ=255)
                                                                                                                                    cattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcgcg                  >  2:1530567/6‑150 (MQ=255)
                                                                                                                                      aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTa                >  1:1126446/1‑150 (MQ=255)
                                                                                                                                      aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGACGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTa                >  2:913006/1‑150 (MQ=255)
                                                                                                                                        gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAAt              >  1:147295/2‑150 (MQ=255)
                                                                                                                                         cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATg             >  1:674094/1‑150 (MQ=255)
                                                                                                                                         cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATg             >  1:1077677/1‑150 (MQ=255)
                                                                                                                                         cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGGAACAGGCTGAGTTCCTGAATGATGCCTATTATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCTTGCGAGCGGAGGTCTCGTTATg             <  2:1138032/150‑1 (MQ=255)
                                                                                                                                         cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTAGAAGGTTGCCTGAAACAGGCTGAGTTCATGAATGATGCCTATGATGATGTGAACTTATAAGAGCGTATTATCGATTCACAATAACCCTGACAGCGGCGTTCGCGTAATg             >  2:1133285/1‑150 (MQ=255)
                                                                                                                                          gCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATgc            >  1:1424024/1‑150 (MQ=255)
                                                                                                                                          gCAAAGCAACCAGGTCGCTTTGCGCAATGGGTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATgc            <  1:725104/150‑1 (MQ=255)
                                                                                                                                               gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGctcc       <  2:1451715/150‑1 (MQ=255)
                                                                                                                                                    cAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGcc  <  2:32370/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCC  >  NC_000913/1499216‑1499513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑