Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,543,229 G→T 6.5% L278M (CTG→ATG)  narU ← nitrate/nitrite transporter NarU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,2290GT 6.5% 336.8 / 6.9 100L278M (CTG→ATG) narUnitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base G (52/41);  new base T (4/3);  total (56/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATG  >  NC_000913/1543081‑1543378
                                                                                                                                                    |                                                                                                                                                      
gCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGc                                                                                                                                                       >  2:370411/1‑150 (MQ=255)
gCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGc                                                                                                                                                       >  1:44025/1‑150 (MQ=255)
gCCATAAAAATAAAGTTGATCAACGTCACCAGCACGCCGCCTAACTTATCGGAAATAGCACCACAAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGc                                                                                                                                                       >  2:534783/1‑150 (MQ=255)
  cATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCCCCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCAt                                                                                                                                                     >  1:789860/1‑150 (MQ=255)
   aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATg                                                                                                                                                    <  2:415556/150‑1 (MQ=255)
    tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                   >  1:620937/1‑150 (MQ=255)
    tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAAAATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                   >  1:656914/1‑150 (MQ=255)
            aaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGGTTTTGCCATGCTGGC‑AAAAacc                                                                                                                                          <  1:1157467/150‑1 (MQ=255)
            aaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTGCCATGCTGGC‑AAAAaccc                                                                                                                                         <  2:323532/150‑2 (MQ=255)
            aaGTTGATCAACGTCACCCGCACGCCGCCGAACCTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTGCCATGCTGGC‑AAAAaccc                                                                                                                                         <  1:1031318/150‑2 (MQ=255)
             aGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAAcccc                                                                                                                                         >  2:812599/1‑149 (MQ=255)
              gTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGc                                                                                                                                        <  2:419475/150‑1 (MQ=255)
               ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCa                                                                                                                                       >  1:1014813/1‑150 (MQ=255)
               ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGCGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCa                                                                                                                                       <  1:243204/150‑1 (MQ=255)
                tGATCAACGTCAACCGCACGCCGCCGAACTTATCGGAATTAGCACCACCAACCGACCGCGCGATGGCCCCGATACATGGGCCAAAGAACGCCAGGCGCTGAATTATCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAACCGCGCAg                                                                                                                                      >  2:1240926/1‑150 (MQ=255)
                 gATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCCCCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGa                                                                                                                                     >  1:139440/1‑150 (MQ=255)
                 gATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGa                                                                                                                                     >  1:1324693/1‑150 (MQ=255)
                 gATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGa                                                                                                                                     >  2:756957/1‑150 (MQ=255)
                  aTCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGaa                                                                                                                                    >  1:289153/1‑150 (MQ=255)
                     aaCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGaaaaa                                                                                                                                 >  2:1007598/1‑150 (MQ=255)
                      aCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAc                                                                                                                                >  1:1267474/1‑150 (MQ=255)
                       cGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAcc                                                                                                                               <  1:933614/150‑1 (MQ=255)
                       cGTCACCAGCACGCCGCCGAACTTATCGGAAATACCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAcc                                                                                                                               <  2:404569/150‑1 (MQ=255)
                          cACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGAt                                                                                                                            >  2:740731/1‑150 (MQ=255)
                            cccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaa                                                                                                                          <  1:54133/150‑1 (MQ=255)
                              cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAAc                                                                                                                        >  2:952043/1‑150 (MQ=255)
                              cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAAc                                                                                                                        <  1:416438/150‑1 (MQ=255)
                              cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAAc                                                                                                                        <  1:417828/150‑1 (MQ=255)
                                          aaCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCAGCGATAAATGGGCCTAAGAAGGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAATCCGATAAACGAACCGAAGGTg                                                                                                            <  2:552750/150‑1 (MQ=255)
                                           aCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTgg                                                                                                           <  2:647117/150‑1 (MQ=255)
                                           aCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTgg                                                                                                           <  1:740731/150‑1 (MQ=255)
                                             ttATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCa                                                                                                         >  1:496662/1‑150 (MQ=255)
                                              tATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCaa                                                                                                        >  2:136407/1‑150 (MQ=255)
                                              tATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGCCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCaa                                                                                                        >  2:1155698/1‑150 (MQ=255)
                                               aTCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAg                                                                                                       <  1:412393/150‑1 (MQ=255)
                                               aTCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAg                                                                                                       <  2:579898/150‑1 (MQ=255)
                                                 cGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGt                                                                                                     <  2:44025/150‑1 (MQ=255)
                                                  ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCATAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    <  1:1313208/150‑1 (MQ=255)
                                                  ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    <  1:1007598/150‑1 (MQ=255)
                                                  ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    <  2:1248019/150‑1 (MQ=255)
                                                  ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    >  1:511184/1‑150 (MQ=255)
                                                         gCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGACCGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAACCAgg                                                                                             >  1:638799/1‑150 (MQ=255)
                                                            ccaccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc                                                                                          >  1:1393089/1‑150 (MQ=255)
                                                            ccaccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc                                                                                          <  1:140219/150‑1 (MQ=255)
                                                             caccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca                                                                                         >  2:253993/1‑150 (MQ=255)
                                                                 aaCCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTcagcag                                                                                     >  2:1240642/1‑150 (MQ=255)
                                                                 aaCCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGA‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTcagcag                                                                                     >  2:1473078/1‑150 (MQ=255)
                                                                   ccgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGcc                                                                                   <  2:458393/150‑1 (MQ=255)
                                                                     gatcgcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCag                                                                                 <  1:660314/147‑1 (MQ=255)
                                                                     gaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCag                                                                                 >  1:638731/1‑150 (MQ=255)
                                                                      accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCaga                                                                                <  1:756957/150‑1 (MQ=255)
                                                                       ccgCGCGATGGCACCGATAAATGGGCCAAAGAACGACAGGCGCAGAATATTGACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCagag                                                                               >  2:329404/1‑150 (MQ=255)
                                                                         gcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGAt                                                                             >  1:1404002/1‑150 (MQ=255)
                                                                           gcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGc                                                                           <  2:927343/150‑1 (MQ=255)
                                                                           gcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGc                                                                           <  1:1216241/150‑1 (MQ=255)
                                                                               tGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGc                                                                       >  1:1518406/1‑150 (MQ=255)
                                                                               tGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGc                                                                       >  1:391666/1‑150 (MQ=255)
                                                                                ggCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCg                                                                      <  2:511184/150‑1 (MQ=255)
                                                                                ggCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCg                                                                      >  1:1222609/1‑150 (MQ=255)
                                                                                  cACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                    >  1:13981/1‑150 (MQ=255)
                                                                                    ccGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGt                                                                  <  1:52028/150‑1 (MQ=255)
                                                                                     cGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTa                                                                 <  1:812599/150‑1 (MQ=255)
                                                                                      gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                >  1:615411/1‑150 (MQ=255)
                                                                                      gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGCTGGCAAGGTAAAGCAGGCTCAGCAGCCAGCGATGCAGGCGTTGTaa                                                                >  2:52702/1‑150 (MQ=255)
                                                                                        tAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGTAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGa                                                              <  1:647274/150‑1 (MQ=255)
                                                                                          aaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                            <  2:1267474/150‑1 (MQ=255)
                                                                                             gggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAggg                                                         <  2:1122837/150‑1 (MQ=255)
                                                                                               gCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                       >  1:623885/1‑150 (MQ=255)
                                                                                                ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                      <  2:638731/150‑1 (MQ=255)
                                                                                                ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                      >  2:937760/1‑150 (MQ=255)
                                                                                                ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                      <  1:927931/150‑1 (MQ=255)
                                                                                                 cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                     >  2:911610/1‑150 (MQ=255)
                                                                                                 cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                     >  2:1325547/1‑150 (MQ=255)
                                                                                                             ggCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTg                                         <  2:151372/150‑1 (MQ=255)
                                                                                                                 cAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                     >  2:1280964/1‑150 (MQ=255)
                                                                                                                  aGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcg                                    <  2:1014813/150‑1 (MQ=255)
                                                                                                                  aGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcg                                    >  2:740494/1‑150 (MQ=255)
                                                                                                                      tatTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTg                                <  2:1404002/150‑1 (MQ=255)
                                                                                                                          cacaTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAAcg                            >  2:1282961/1‑149 (MQ=255)
                                                                                                                          cacaTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACt                            <  2:1324693/150‑1 (MQ=255)
                                                                                                                          cacaTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACt                            >  2:743063/1‑150 (MQ=255)
                                                                                                                            caTCCGGGAACTGGGTTTTTGCCAGAATCGC‑AAAACCCGCAGAAAAACCGATAAACGAAACGAACCTGGCAAGCTAAAGAAGGCTCAGCAGCCAGAGATGCAGGCGTAGTAAGACACGGAGCTGGTCGGCAATTGAGGCGCGTGAACTgg                          >  2:116606/1‑150 (MQ=255)
                                                                                                                             aTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                         <  1:952043/150‑1 (MQ=255)
                                                                                                                              tCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCTGCAATTGAGGCGCGTGAACTGGCg                        <  2:13981/150‑1 (MQ=255)
                                                                                                                                cGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGat                      >  1:127685/1‑150 (MQ=255)
                                                                                                                                  ggAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGatat                    <  1:566424/150‑1 (MQ=255)
                                                                                                                                   taaCTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtc                   <  2:642624/149‑1 (MQ=255)
                                                                                                                                       tGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattc               >  2:132172/1‑150 (MQ=255)
                                                                                                                                        gggTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattca              >  2:460056/1‑150 (MQ=255)
                                                                                                                                        cgggttttGCCATGCTGGCAAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattc               >  2:220985/5‑150 (MQ=255)
                                                                                                                                         gggttttGCCATGCTGGC‑AAAACCCGCAGAAAAACCGATAAACGCACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat             >  1:1409367/4‑150 (MQ=255)
                                                                                                                                         gggttttGCCATGCTGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat             >  1:1353403/4‑150 (MQ=255)
                                                                                                                                          ggttttGCCATGCTGGCAAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat             >  2:461163/3‑150 (MQ=255)
                                                                                                                                            ttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc          <  1:1155698/150‑1 (MQ=255)
                                                                                                                                               tGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGa       >  2:454116/1‑150 (MQ=255)
                                                                                                                                                gCCAGCATGGC‑AAAACCCGCAGAAAACCCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCNANAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGNACTGGCGATATCATTCATNCCTGAc      >  2:1212877/1‑150 (MQ=255)
                                                                                                                                                gCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGAc      >  1:1423673/1‑150 (MQ=255)
                                                                                                                                                gCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGAc      >  1:1217510/1‑150 (MQ=255)
                                                                                                                                                 ccAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGAcc     >  1:710342/1‑150 (MQ=255)
                                                                                                                                                    gCATGGC‑AAAACCCGCAGAAAACCCGATCAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTCAGACAGTGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCTTTCATCCCTGACCATg  <  2:656914/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATG  >  NC_000913/1543081‑1543378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑