BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	192,579	A→T	5.7%	*242L (TAA→TTA)	pyrH →	UMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,579	0	A	T	5.7%	331.5 / 7.2	106	*242L (TAA→TTA)	pyrH	UMP kinase
Reads supporting (aligned to +/- strand): ref base A (56/44); new base T (3/3); total (59/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 108 total aligned reads displayed.

GGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGC  >  NC_000913/192433‑192726

ggAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCATTATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAAtt                                                                                                                                                  >  1:903456/1‑150 (MQ=255)

ggAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGATTAAtt                                                                                                                                                  >  1:687423/1‑150 (MQ=255)

ggAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAAtt                                                                                                                                                  >  2:623987/1‑150 (MQ=255)

ggAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAAtt                                                                                                                                                  <  2:1429737/150‑1 (MQ=255)

ggAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAAtt                                                                                                                                                  >  2:821247/1‑150 (MQ=255)

ggAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAACGGACTTTAATCAC‑GGAATAAtt                                                                                                                                                  >  2:1150504/1‑150 (MQ=255)

     aaGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGt                                                                                                                                             >  1:11042/1‑150 (MQ=255)

     aaGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGt                                                                                                                                             <  2:1036023/150‑1 (MQ=255)

      agagCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTg                                                                                                                                            >  1:43046/1‑150 (MQ=255)

        agCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGAt                                                                                                                                          <  1:631179/150‑1 (MQ=255)

        agCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGAt                                                                                                                                          <  1:626035/150‑1 (MQ=255)

         tcTGAAAGTCATGGAGCTGGCGTCCTTCACGGTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGTGTGCGCTGCGCCGTTTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATg                                                                                                                                         <  2:43046/149‑1 (MQ=255)

         gCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATg                                                                                                                                         >  1:595310/1‑150 (MQ=255)

         gCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATg                                                                                                                                         <  1:1346092/150‑1 (MQ=255)

          cTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCACGGGAATTATT‑CCGTGAtta                                                                                                                                        <  2:135072/150‑3 (MQ=255)

           tGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCACGGGAATTATT‑CCGTGAttaa                                                                                                                                       <  1:681887/150‑4 (MQ=255)

           tGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGa                                                                                                                                       >  1:381118/1‑150 (MQ=255)

                gTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaat                                                                                                                                  <  2:861625/150‑1 (MQ=255)

                  cATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaataa                                                                                                                                >  1:1506310/1‑150 (MQ=255)

                       aCCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCATTATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTa                                                                                                                           <  2:903456/150‑1 (MQ=255)

                       aCCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTa                                                                                                                           <  2:1383606/150‑1 (MQ=255)

                          tGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGa                                                                                                                        >  1:1002989/1‑150 (MQ=255)

                               gcCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcg                                                                                                                   <  1:144029/150‑1 (MQ=255)

                                ccTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgc                                                                                                                  >  2:552472/1‑150 (MQ=255)

                                 cTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgcg                                                                                                                 <  2:256138/150‑1 (MQ=255)

                                  ttCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGt                                                                                                                >  2:1142526/1‑150 (MQ=255)

                                   tCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGTGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTa                                                                                                               <  1:610070/150‑1 (MQ=255)

                                    cACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTaa                                                                                                              >  2:865315/1‑150 (MQ=255)

                                        cTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTAt                                                                                                          >  1:251749/1‑150 (MQ=255)

                                        cTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTAt                                                                                                          <  2:793022/150‑1 (MQ=255)

                                           gCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATcgc                                                                                                       >  2:1535248/1‑150 (MQ=255)

                                                  aCCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCg                                                                                                <  1:1100824/150‑1 (MQ=255)

                                                   ccATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGt                                                                                               >  1:125886/1‑150 (MQ=255)

                                                    cATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTTCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTa                                                                                              <  1:851814/150‑1 (MQ=255)

                                                    cATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTa                                                                                              <  2:472782/150‑1 (MQ=255)

                                                    cATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTa                                                                                              <  1:552472/150‑1 (MQ=255)

                                                      tAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAg                                                                                            <  1:865315/150‑1 (MQ=255)

                                                         aTTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCt                                                                                         >  2:627227/1‑150 (MQ=255)

                                                            aCCGATTCGTGTTTTCAATATGAAGAAACCGGGTGCGCTGCGCCGTGTGGTAATGTGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGTGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGt                                                                                      <  2:422599/150‑1 (MQ=255)

                                                            aCCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGt                                                                                      <  1:1092689/150‑1 (MQ=255)

                                                                      gtTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGt                                                                            >  1:379191/1‑150 (MQ=255)

                                                                          tCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAtt                                                                        >  2:569015/1‑150 (MQ=255)

                                                                           cAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttt                                                                       >  2:620449/1‑149 (MQ=255)

                                                                           cAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttg                                                                       <  2:1086004/150‑1 (MQ=255)

                                                                           cAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttg                                                                       >  2:1051726/1‑150 (MQ=255)

                                                                              tatGAACAAACCGGGTGGGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttgttg                                                                    >  2:961255/1‑150 (MQ=255)

                                                                               atGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgc                                                                   <  2:1168202/150‑1 (MQ=255)

                                                                                tGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgca                                                                  <  2:381118/150‑1 (MQ=255)

                                                                                tGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgca                                                                  >  2:799488/1‑150 (MQ=255)

                                                                                 gAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCTGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcag                                                                 >  1:1101178/1‑150 (MQ=255)

                                                                                 gAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcag                                                                 <  1:185928/150‑1 (MQ=255)

                                                                                 gAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcag                                                                 >  2:1348243/1‑150 (MQ=255)

                                                                                 gAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcag                                                                 >  2:287895/1‑150 (MQ=255)

                                                                                 gAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCAGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGCCGTTATTGTTgcag                                                                 >  1:1234566/1‑150 (MQ=255)

                                                                                  aaCAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcagg                                                                >  1:848740/1‑150 (MQ=255)

                                                                                   aCAAACCGGGTGCGCTGCGCCGTGAGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCCTGATGGATAAATAAGGGTAAGATTCCGCCTAAGTATCCCGGCGGCGTAAGTGAGGTTATAAGGCGTTATTGTTccagga                                                               >  2:1338276/1‑149 (MQ=255)

                                                                                      aaCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGt                                                            >  2:935469/1‑150 (MQ=255)

                                                                                      aaCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGt                                                            >  2:1088791/1‑150 (MQ=255)

                                                                                       aCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGtt                                                           <  2:556885/150‑1 (MQ=255)

                                                                                          gggTGCGCTGCGCCGTGTGGTAATGTGTGAAAAATAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTgg                                                        <  1:523793/150‑1 (MQ=255)

                                                                                          gggTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTgg                                                        <  1:287895/150‑1 (MQ=255)

                                                                                           ggTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGt                                                       <  1:222591/150‑1 (MQ=255)

                                                                                           ggTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGt                                                       <  1:1404406/150‑1 (MQ=255)

                                                                                              gcgctgcgcCGTGTGGTAATGGGTGAAAAACAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCAc                                                    <  2:228194/150‑1 (MQ=255)

                                                                                                 ctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGc                                                 >  1:1033416/1‑150 (MQ=255)

                                                                                                 ctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGc                                                 <  2:501359/150‑1 (MQ=255)

                                                                                                 ctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGc                                                 >  2:788087/1‑150 (MQ=255)

                                                                                                   gcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCa                                               >  1:353355/1‑150 (MQ=255)

                                                                                                            ggTAATGGGTGAAAAAGAAGGGAGTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCgcagc                                      >  1:205184/1‑150 (MQ=255)

                                                                                                             gTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCgcagca                                     >  2:706197/1‑150 (MQ=255)

                                                                                                              tAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCaa                                    >  1:1459997/1‑150 (MQ=255)

                                                                                                                 tGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGAGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCg                                 >  2:385359/1‑150 (MQ=255)

                                                                                                                  gggTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCgg                                <  2:1242497/150‑1 (MQ=255)

                                                                                                                    gTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAg                              >  2:492343/1‑150 (MQ=255)

                                                                                                                        aaaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTa                          >  2:870922/1‑150 (MQ=255)

                                                                                                                        aaaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTa                          <  1:965191/150‑1 (MQ=255)

                                                                                                                         aaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTAc                         <  1:627227/150‑1 (MQ=255)

                                                                                                                           agaagGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACaa                       >  2:1056159/1‑150 (MQ=255)

                                                                                                                              agGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAg                    >  1:1022456/1‑150 (MQ=255)

                                                                                                                               gggACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGt                   >  1:185132/1‑150 (MQ=255)

                                                                                                                                   tatccaTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGt               >  2:904072/6‑150 (MQ=255)

                                                                                                                                   cTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGt               <  1:1150504/150‑1 (MQ=255)

                                                                                                                                   cTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGt               >  1:368134/1‑150 (MQ=255)

                                                                                                                                   cTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGt               >  1:619368/1‑150 (MQ=255)

                                                                                                                                    atccaTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTg              >  1:512051/5‑150 (MQ=255)

                                                                                                                                      tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAATATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGTCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTActttag            <  2:1101178/150‑6 (MQ=255)

                                                                                                                                      tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAg            >  2:961812/1‑150 (MQ=255)

                                                                                                                                      tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAg            >  1:993389/1‑150 (MQ=255)

                                                                                                                                      tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAg            >  1:1020949/1‑150 (MQ=255)

                                                                                                                                       caTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAgg           >  1:544554/2‑150 (MQ=255)

                                                                                                                                       aaTCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAATATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAgg           >  2:564074/1‑150 (MQ=255)

                                                                                                                                        aTCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGa          <  1:620449/150‑1 (MQ=255)

                                                                                                                                          cAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATg        >  2:304981/1‑150 (MQ=255)

                                                                                                                                            cgggAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGc      <  1:904072/148‑1 (MQ=255)

                                                                                                                                            c‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGc      <  1:799488/150‑1 (MQ=255)

                                                                                                                                            c‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGc      >  1:660367/1‑150 (MQ=255)

                                                                                                                                              ggAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCg     <  2:1459997/150‑1 (MQ=255)

                                                                                                                                              ggAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCg     <  1:1133122/150‑1 (MQ=255)

                                                                                                                                                aataatTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAg   <  1:1348243/150‑1 (MQ=255)

                                                                                                                                                 ataatTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGc  >  1:1068647/1‑150 (MQ=255)

GGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGC  >  NC_000913/192433‑192726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`