Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,698,587 T→A 7.4% A198A (GCA→GCT) ‡ malI ← DNA‑binding transcriptional repressor MalI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,698,5870TA 7.4% 201.2 / 3.8 66A198A (GCA→GCT) ‡malIDNA‑binding transcriptional repressor MalI
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base A (2/3);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CCACGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAA  >  NC_000913/1698439‑1698736
                                                                                                                                                    |                                                                                                                                                     
ccACGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTg                                                                                                                                                      <  2:388683/150‑1 (MQ=255)
   cGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCAc                                                                                                                                                   >  1:1491093/1‑150 (MQ=255)
    ggCACTGCTGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCCATCGGTATGAACCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCTACCCGTTCTGCACg                                                                                                                                                  <  2:15714/150‑1 (MQ=255)
      cACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCGAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACggg                                                                                                                                                <  2:751133/150‑1 (MQ=255)
        cTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATTAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGtt                                                                                                                                              <  2:1107445/150‑1 (MQ=255)
        cTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGtt                                                                                                                                              <  1:30822/150‑1 (MQ=255)
          taTGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTGCAGAACGGGttgg                                                                                                                                            <  1:1243633/149‑3 (MQ=255)
            tGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATg                                                                                                                                          >  1:833582/1‑150 (MQ=255)
            gggTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCGGGAAGGGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTGCAGACCGGGttgggg                                                                                                                                          <  1:1528116/149‑5 (MQ=255)
             ggTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTGCAGAACGGGttggggg                                                                                                                                         <  1:559669/150‑6 (MQ=255)
                cGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGa                                                                                                                                      >  2:984722/1‑150 (MQ=255)
                cGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGa                                                                                                                                      >  2:710670/1‑150 (MQ=255)
                   gTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACt                                                                                                                                   >  1:1467312/1‑150 (MQ=255)
                       tGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGc                                                                                                                               <  1:1385052/150‑1 (MQ=255)
                         aCGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGccc                                                                                                                             <  2:547476/150‑1 (MQ=255)
                           gTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTc                                                                                                                           >  1:855551/1‑150 (MQ=255)
                             aaaaGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTccc                                                                                                                         <  1:729450/150‑1 (MQ=255)
                                     cGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACTGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGc                                                                                                                 <  2:1491093/150‑1 (MQ=255)
                                       tGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCACCACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCCGCCAGGCGa                                                                                                               >  1:1436345/1‑150 (MQ=255)
                                        gATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGAt                                                                                                              >  2:57611/1‑150 (MQ=255)
                                          tAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCACAGCCAGGCGATcc                                                                                                            >  1:610282/1‑150 (MQ=255)
                                            gCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCAAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGAACAATAGCCCCCAACCCGTTATGCACGGGTTAATGAGTAACTTTGCACTCCCAGCCACGCGATCCgg                                                                                                          >  2:296218/1‑149 (MQ=255)
                                             cTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCACCACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGCACTTTGCCCTCCCCGCCCGGCGATCCGCt                                                                                                         >  1:303190/1‑150 (MQ=255)
                                                ccGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGAt                                                                                                      >  1:883584/1‑150 (MQ=255)
                                                 cgcgGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATg                                                                                                     >  2:188775/1‑150 (MQ=255)
                                                 cgcgGCTTGCTTCTGGCGGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATg                                                                                                     <  2:339579/150‑1 (MQ=255)
                                                     gcttgcttCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCa                                                                                                 <  1:1459961/150‑1 (MQ=255)
                                                         gcttCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGc                                                                                             <  2:833582/150‑1 (MQ=255)
                                                           ttCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACAGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGa                                                                                           >  1:1492961/1‑150 (MQ=255)
                                                             ctggctggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt                                                                                         >  2:632886/1‑150 (MQ=255)
                                                                 ctggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATgaga                                                                                     <  1:477154/150‑1 (MQ=255)
                                                                     aaGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCt                                                                                 <  1:771789/150‑1 (MQ=255)
                                                                     aaGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCt                                                                                 <  2:1467312/150‑1 (MQ=255)
                                                                              ccAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca                                                                        >  2:204401/1‑150 (MQ=255)
                                                                              ccAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca                                                                        <  1:1157093/150‑1 (MQ=255)
                                                                                aaCACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCaacaac                                                                      >  1:981718/1‑150 (MQ=255)
                                                                                aaCACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCaacaac                                                                      >  1:1147161/1‑150 (MQ=255)
                                                                                    cccAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg                                                                  >  1:916215/1‑150 (MQ=255)
                                                                                    cccAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg                                                                  <  1:710670/150‑1 (MQ=255)
                                                                                    cccAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg                                                                  >  2:378276/1‑150 (MQ=255)
                                                                                         tCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGcc                                                             >  2:601774/1‑150 (MQ=255)
                                                                                           gCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg                                                           >  1:1057361/1‑150 (MQ=255)
                                                                                            cTATGAAACTGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGc                                                          <  2:1492961/150‑1 (MQ=255)
                                                                                                 aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgtt                                                     >  2:1186988/1‑150 (MQ=255)
                                                                                                      gCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCg                                                <  2:294066/150‑1 (MQ=255)
                                                                                                        aGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCggg                                              <  2:1316534/150‑1 (MQ=255)
                                                                                                        aGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCggg                                              >  2:848119/1‑150 (MQ=255)
                                                                                                         ggCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGc                                             >  2:217732/1‑150 (MQ=255)
                                                                                                           ccAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGa                                           >  1:1270290/1‑150 (MQ=255)
                                                                                                            cAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGaa                                          >  2:1389794/1‑150 (MQ=255)
                                                                                                                 tttAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTa                                     <  1:204401/150‑1 (MQ=255)
                                                                                                                     gtagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCaa                                 <  2:1193021/150‑1 (MQ=255)
                                                                                                                     gtagAGTTGCACAATAGCCCCCAACCCGCGCTGCACGGGTTAATGAGGAACTTTCCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCaa                                 <  2:1535785/150‑1 (MQ=255)
                                                                                                                       agagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAca                               <  1:1186988/150‑1 (MQ=255)
                                                                                                                            tGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc                          >  2:705207/1‑150 (MQ=255)
                                                                                                                               acaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg                       <  1:57611/150‑1 (MQ=255)
                                                                                                                                  aTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATa                    >  1:1455509/1‑150 (MQ=255)
                                                                                                                                  aTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATa                    <  2:790203/150‑1 (MQ=255)
                                                                                                                                     gCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTTGGTTAACTTCTGTGGAGCCTGCATGTTGTTCGGGGGTACTGTATGATTTTCATCAAGTTatgt                 <  2:730345/150‑4 (MQ=255)
                                                                                                                                     ctcattaaCCCGTGCAGAACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACt                 >  1:758488/7‑150 (MQ=255)
                                                                                                                                     ctcattaaCCCGTGCAGAACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACt                 >  2:910822/7‑150 (MQ=255)
                                                                                                                                       ccccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg               >  1:22240/1‑150 (MQ=255)
                                                                                                                                       ccccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg               >  1:1211493/1‑150 (MQ=255)
                                                                                                                                       ccccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAACGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg               >  2:282827/1‑150 (MQ=255)
                                                                                                                                            aCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACgg          <  1:475326/150‑1 (MQ=255)
                                                                                                                                                   cTGAACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGa   >  1:786053/1‑150 (MQ=255)
                                                                                                                                                    tGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGaa  <  1:984722/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCACGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGGGAAGCGAA  >  NC_000913/1698439‑1698736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑