Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,829,334	G→T	10.6%	P134Q (CCG→CAG)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,334	0	G	T	10.6%	202.4 / 11.0	66	P134Q (CCG→CAG)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (36/23);  new base T (4/3);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

AAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTG  >  NC_000913/1829187‑1829483                                                                                                                                                    |                                                                                                                                                      aaaCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCggg                                                                                                                                                     >  2:101517/1‑150 (MQ=255)  aaCTCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGNCGTAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGc                                                                                                                                                    <  2:660892/150‑1 (MQ=255)  aaCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACATCGCCCGCACGGCGTGCTGGc                                                                                                                                                    <  2:1177435/150‑1 (MQ=255)    cGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCgg                                                                                                                                                  <  2:457022/150‑2 (MQ=255)       aTTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGt                                                                                                                                               >  2:624405/1‑150 (MQ=255)         tACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCCCGCCGTGCGGGCGATGTcc                                                                                                                                             >  2:1501753/1‑149 (MQ=255)           ccGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCa                                                                                                                                           >  2:812250/1‑150 (MQ=255)           ccGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCa                                                                                                                                           >  2:305198/1‑150 (MQ=255)             gCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCGgtgtttgggc                                                                                                                                         <  1:746808/150‑7 (MQ=255)                 cGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcg                                                                                                                                     <  2:186230/150‑1 (MQ=255)                  gCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgc                                                                                                                                    <  1:624405/150‑1 (MQ=255)                        ccACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcgcc                                                                                                                              >  2:1145688/1‑150 (MQ=255)                         cACGGTGTCAGTTCGCTGGGTTTAAAGATATTGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcgccg                                                                                                                             >  2:1279671/1‑150 (MQ=255)                           cGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTc                                                                                                                           >  2:193827/1‑150 (MQ=255)                              tgtCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTTGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCgg                                                                                                                        >  1:685631/1‑150 (MQ=255)                                     tCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCa                                                                                                                 <  1:1431461/150‑1 (MQ=255)                                      cGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCAc                                                                                                                >  1:1283540/1‑150 (MQ=255)                                       gCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACt                                                                                                               >  1:510542/1‑150 (MQ=255)                                        cTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTa                                                                                                              <  2:1194918/150‑1 (MQ=255)                                               tAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgctgct                                                                                                       >  2:379009/1‑150 (MQ=255)                                               tAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgctgct                                                                                                       >  2:114986/1‑150 (MQ=255)                                                        tGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTAc                                                                                              >  2:1075174/1‑150 (MQ=255)                                                          gtgtTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGa                                                                                            >  2:952239/1‑150 (MQ=255)                                                              tACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGt                                                                                        >  2:697948/1‑150 (MQ=255)                                                              tACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAAGGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGt                                                                                        >  1:90646/1‑150 (MQ=255)                                                               aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTg                                                                                       >  2:438030/1‑150 (MQ=255)                                                                ccTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGa                                                                                      <  2:917586/150‑1 (MQ=255)                                                                  tGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                                    >  1:597058/1‑150 (MQ=255)                                                                  tGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                                    >  1:1446641/1‑150 (MQ=255)                                                                     cAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGc                                                                                 <  2:955531/150‑1 (MQ=255)                                                                         aaTGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTa                                                                             >  1:42721/1‑150 (MQ=255)                                                                           tGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAAt                                                                           <  1:896562/150‑1 (MQ=255)                                                                           tGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAAt                                                                           >  2:859876/1‑150 (MQ=255)                                                                             ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                         <  1:930586/150‑1 (MQ=255)                                                                             ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                         >  2:617325/1‑150 (MQ=255)                                                                             ccGGAACGATATGTCCGTTCGGCAAATGACCAGGCAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                         <  1:1305560/150‑1 (MQ=255)                                                                                    gATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcgc                                                                  >  1:1057347/1‑150 (MQ=255)                                                                                      tatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGa                                                                >  1:1209834/1‑150 (MQ=255)                                                                                      tatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGa                                                                >  2:612921/1‑150 (MQ=255)                                                                                         gTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCCTTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGAttt                                                             >  2:1279824/1‑150 (MQ=255)                                                                                         gTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGAttt                                                             <  2:1188171/150‑1 (MQ=255)                                                                                              ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTg                                                        <  2:1259760/150‑1 (MQ=255)                                                                                                 ggCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGatc                                                     <  1:251704/150‑1 (MQ=255)                                                                                                  gCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGatca                                                    <  1:697948/150‑1 (MQ=255)                                                                                                   cAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGatcat                                                   >  1:62458/1‑150 (MQ=255)                                                                                                      aTGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGc                                                <  1:34082/150‑1 (MQ=255)                                                                                                         aCCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGt                                             >  1:1091604/1‑150 (MQ=255)                                                                                                            aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGATCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCAc                                          >  2:22570/1‑150 (MQ=255)                                                                                                              ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACtt                                        >  1:1389496/1‑150 (MQ=255)                                                                                                                 aaTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCgg                                     >  2:735125/1‑150 (MQ=255)                                                                                                                  aTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGt                                    <  1:379009/150‑1 (MQ=255)                                                                                                                  aTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGt                                    <  1:812250/150‑1 (MQ=255)                                                                                                                     ataCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGc                                 >  2:305033/1‑150 (MQ=255)                                                                                                                      taCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGcc                                <  1:1337413/150‑1 (MQ=255)                                                                                                                         ggCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCt                             >  2:683260/1‑150 (MQ=255)                                                                                                                         gcctgcgacatCGCCCGCACGGCGTGCTGGCGGTGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCt                             >  2:1139553/8‑150 (MQ=39)                                                                                                                         gcctgcgacatCGCCCGCACGGCGTGCTGGCGGTGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCt                             >  2:1144913/8‑150 (MQ=39)                                                                                                                            tgcgacatCGCCCGCACGGCGTGCTGGCGGTGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTcc                          >  2:527902/5‑150 (MQ=255)                                                                                                                            ccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTcc                          <  2:685631/150‑1 (MQ=255)                                                                                                                                 catcGCCCGCACGGCGTGCTGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgcg                     >  1:1284647/4‑150 (MQ=255)                                                                                                                                   ccGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGcgcg                   >  1:703686/1‑150 (MQ=255)                                                                                                                                     gCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGc                 <  1:114986/150‑1 (MQ=255)                                                                                                                                       cAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCtt               <  2:171637/150‑1 (MQ=255)                                                                                                                                       cAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCtt               <  2:709213/150‑1 (MQ=255)                                                                                                                                         gCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTAc             <  1:814889/150‑1 (MQ=255)                                                                                                                                               cGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGttt       >  2:595380/1‑150 (MQ=255)                                                                                                                                                  gCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTtctc    >  2:471487/1‑150 (MQ=255)                                                                                                                                                    gggCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTg  >  2:1430987/1‑150 (MQ=255)                                                                                                                                                    gggCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTg  <  2:510542/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTG  >  NC_000913/1829187‑1829483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑