Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,851,206 G→T 7.5% G116V (GGG→GTG) ‡ ansA → L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,851,2060GT 7.5% 260.5 / 7.6 80G116V (GGG→GTG) ‡ansAL‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base G (33/41);  new base T (3/3);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

AGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATG  >  NC_000913/1851057‑1851355
                                                                                                                                                     |                                                                                                                                                     
aGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAgg                                                                                                                                                       >  2:660570/1‑150 (MQ=255)
  aCTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGt                                                                                                                                                     <  1:819434/150‑1 (MQ=255)
   cTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTc                                                                                                                                                    <  2:709860/150‑1 (MQ=255)
      gcaggaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTTTCATTCTGCACGGAACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTcaca                                                                                                                                                 <  2:810105/150‑1 (MQ=255)
       cagcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACaa                                                                                                                                                >  1:531850/1‑150 (MQ=255)
         gcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatg                                                                                                                                              <  1:666510/150‑3 (MQ=255)
         gcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatg                                                                                                                                              <  1:1177070/150‑3 (MQ=255)
         gcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAAt                                                                                                                                              >  1:412337/1‑150 (MQ=255)
             atTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatgaccg                                                                                                                                          <  1:86681/150‑7 (MQ=255)
              ttGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgc                                                                                                                                         >  1:524885/1‑150 (MQ=255)
               tGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgct                                                                                                                                        <  1:55670/150‑1 (MQ=255)
                gCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctg                                                                                                                                       >  1:1347955/1‑150 (MQ=255)
                  tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggc                                                                                                                                     >  1:1246334/1‑150 (MQ=255)
                  tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggc                                                                                                                                     <  2:1293255/150‑1 (MQ=255)
                    aaGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggctg                                                                                                                                   >  2:642508/1‑150 (MQ=255)
                    aaGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggctg                                                                                                                                   <  2:179662/150‑1 (MQ=255)
                    aaGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCAAGGAACCGACAAGATGGCGTATACCGCCTCTGAGCTGTCGTTCATGAACGAGAATCTCGGAAAACCGGTCAATGTGACAGGGTCAAAAATCCCgctggctg                                                                                                                                   >  2:1410878/1‑150 (MQ=255)
                      gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAg                                                                                                                                 <  1:162889/150‑1 (MQ=255)
                        tatTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGtt                                                                                                                               >  1:983437/1‑150 (MQ=255)
                              aGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGctc                                                                                                                         <  2:154337/150‑1 (MQ=255)
                                   aCTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACg                                                                                                                    <  2:1170570/150‑1 (MQ=255)
                                   aCTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACg                                                                                                                    <  2:1165003/150‑1 (MQ=255)
                                               atgatgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATc                                                                                                        <  1:1392729/150‑1 (MQ=255)
                                                   tgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgct                                                                                                    <  1:657384/150‑1 (MQ=255)
                                                   tgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgct                                                                                                    >  1:37168/1‑150 (MQ=255)
                                                    ggTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgctg                                                                                                   <  1:962110/150‑1 (MQ=255)
                                                    ggTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgctg                                                                                                   >  2:1459469/1‑150 (MQ=255)
                                                    ggTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgctg                                                                                                   <  2:531850/150‑1 (MQ=255)
                                                     gTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGa                                                                                                  <  2:802533/150‑1 (MQ=255)
                                                      ttttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGaa                                                                                                 <  1:1002752/150‑1 (MQ=255)
                                                          gTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGGTCATGCTCGAGAAGCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTCCGCTCGGACGGACAAATTAATCTGCTGAATGCg                                                                                             >  2:1486950/1‑150 (MQ=255)
                                                             aTTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTg                                                                                          <  1:482117/150‑1 (MQ=255)
                                                             aTTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTg                                                                                          >  1:1424365/1‑150 (MQ=255)
                                                             aTTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTg                                                                                          <  1:737352/150‑1 (MQ=255)
                                                             aTTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTg                                                                                          <  1:65944/150‑1 (MQ=255)
                                                               tCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTa                                                                                        <  1:837939/150‑1 (MQ=255)
                                                                 tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACg                                                                                      >  2:1074532/1‑150 (MQ=255)
                                                                 tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACg                                                                                      >  1:865122/1‑150 (MQ=255)
                                                                 tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACg                                                                                      <  1:1052650/150‑1 (MQ=255)
                                                                   cACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGtt                                                                                    >  1:520077/1‑150 (MQ=255)
                                                                    aCGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTg                                                                                   <  1:457271/150‑1 (MQ=255)
                                                                        cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcggc                                                                               >  1:653764/1‑150 (MQ=255)
                                                                        cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcggc                                                                               <  1:1074532/150‑1 (MQ=255)
                                                                            gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                           <  2:685480/150‑1 (MQ=255)
                                                                            gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                           >  1:1460674/1‑150 (MQ=255)
                                                                            gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                           <  1:1424523/150‑1 (MQ=255)
                                                                              cacGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTa                                                                         <  1:1117681/150‑1 (MQ=255)
                                                                                 gATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATcc                                                                      <  2:1408728/150‑1 (MQ=255)
                                                                                       gTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCaa                                                                <  1:656424/150‑1 (MQ=255)
                                                                                         ataCCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACg                                                              <  1:1459469/150‑1 (MQ=255)
                                                                                            ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAg                                                           <  2:291018/150‑1 (MQ=255)
                                                                                            ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAg                                                           >  2:1414415/1‑150 (MQ=255)
                                                                                             cGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGt                                                          >  1:650190/1‑150 (MQ=255)
                                                                                             cGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGt                                                          >  1:1523518/1‑150 (MQ=255)
                                                                                                        tGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCtttt                                               <  1:1533042/150‑1 (MQ=255)
                                                                                                         gTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCttttt                                              >  1:587669/1‑150 (MQ=255)
                                                                                                           ggTTCATGCTCGAGAATCTCGGTATACCGGTCATTTTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGATGAATGCGTTGTACGTTGCGGCTAATTATCCGATCAACGAAGTAACGCTCTTTTTCa                                            <  2:412337/149‑1 (MQ=255)
                                                                                                           cGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCa                                            <  1:1270243/150‑1 (MQ=255)
                                                                                                             ttCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAAt                                          >  1:921196/1‑150 (MQ=255)
                                                                                                               cATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTATTTCAATaa                                        <  2:983437/150‑1 (MQ=255)
                                                                                                                 tGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc                                      >  1:1481253/1‑150 (MQ=255)
                                                                                                                  gCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCg                                     >  1:344791/1‑150 (MQ=255)
                                                                                                                   cTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                    >  1:173072/1‑150 (MQ=255)
                                                                                                                    tCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATATCCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                                   >  2:1463958/1‑150 (MQ=255)
                                                                                                                           tctcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                                   <  1:51396/143‑1 (MQ=255)
                                                                                                                           tctcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                                   >  2:51396/1‑143 (MQ=255)
                                                                                                                             tcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg                          >  1:907129/1‑150 (MQ=255)
                                                                                                                                    aCCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg                   >  2:934575/1‑150 (MQ=255)
                                                                                                                                    aCCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg                   <  2:1080913/150‑1 (MQ=255)
                                                                                                                                       ggTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc                >  2:60833/1‑150 (MQ=255)
                                                                                                                                       ggTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc                >  1:1442690/1‑150 (MQ=255)
                                                                                                                                        ggatttGTGACCCTGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACt               >  2:286465/5‑150 (MQ=255)
                                                                                                                                        ggatttGTGACCCTGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACt               >  1:216205/5‑150 (MQ=255)
                                                                                                                                        ggatttGTGACCCTGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACt               >  1:3636/5‑150 (MQ=255)
                                                                                                                                        gTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACt               <  1:642508/150‑1 (MQ=255)
                                                                                                                                           aTTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTAcc            <  2:1460674/150‑1 (MQ=255)
                                                                                                                                            ttGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACAAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCa           <  2:524885/150‑1 (MQ=255)
                                                                                                                                              gtgACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaaa         >  2:1468082/1‑150 (MQ=255)
                                                                                                                                               tgACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAg        <  2:482118/150‑1 (MQ=255)
                                                                                                                                                gACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGc       >  1:449739/1‑150 (MQ=255)
                                                                                                                                                gACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGc       <  2:921196/150‑1 (MQ=255)
                                                                                                                                                    gggTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCAt   >  2:1281554/1‑150 (MQ=255)
                                                                                                                                                    gggTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGAGGCAACCGCACTACCAAAGCCCAt   >  1:695515/1‑150 (MQ=255)
                                                                                                                                                     ggTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATg  >  1:57384/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATG  >  NC_000913/1851057‑1851355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑