Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,962,270	C→G	5.7%	A100G (GCC→GGC)	yecT →	protein YecT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,270	0	C	G	5.7%	326.4 / 7.1	99	A100G (GCC→GGC)	yecT	protein YecT
Reads supporting (aligned to +/- strand):  ref base C (43/48);  new base G (4/2);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTATACCG  >  NC_000913/1962121‑1962417                                                                                                                                                      |                                                                                                                                                     aTCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGAGAGc                                                                                                                                                      >  1:295526/1‑150 (MQ=255)        cTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCctggctg                                                                                                                                               <  1:37276/150‑1 (MQ=255)          gCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTCTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑Ga                                                                                                                                            >  2:1164727/1‑150 (MQ=255)            gACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GAtt                                                                                                                                          <  2:980432/150‑1 (MQ=255)            gACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GAtt                                                                                                                                          >  1:434866/1‑150 (MQ=255)             aCAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GAttt                                                                                                                                         >  2:474543/1‑150 (MQ=255)              cAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GAtttt                                                                                                                                        <  2:1151535/150‑1 (MQ=255)               aaGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTa                                                                                                                                       <  1:1466175/150‑1 (MQ=255)                aGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAg                                                                                                                                      <  2:785461/150‑1 (MQ=255)                aGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAg                                                                                                                                      >  2:414160/1‑150 (MQ=255)                aGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTCTAg                                                                                                                                      <  1:694626/150‑1 (MQ=255)                 gAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAgg                                                                                                                                     >  1:942803/1‑150 (MQ=255)                 gAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAgg                                                                                                                                     <  2:1043760/150‑1 (MQ=255)                  aaTACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAggg                                                                                                                                    <  2:1382599/150‑1 (MQ=255)                  aaTACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAggg                                                                                                                                    <  1:59123/150‑1 (MQ=255)                      cAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACaa                                                                                                                                >  1:879751/1‑150 (MQ=255)                               cgATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGa                                                                                                                       <  2:1453206/150‑1 (MQ=255)                                gATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGaa                                                                                                                      <  1:1501057/150‑1 (MQ=255)                                    cgcAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTaa                                                                                                                  >  1:1222664/1‑150 (MQ=255)                                      cAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATc                                                                                                                >  2:594461/1‑150 (MQ=255)                                         ttAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTTTATTTCTTTAAAATTCTAAGCGAGACTGGTTG‑TATTTTAGTGACAAAGAATGTTAATTAATCCtt                                                                                                             <  2:624252/150‑1 (MQ=255)                                         ttAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCtt                                                                                                             >  1:498625/1‑150 (MQ=255)                                          taaataaaGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCttt                                                                                                            >  2:284441/1‑150 (MQ=255)                                          taaataaaGCCTATGCTGAAGCTTATCGTTATATAGAACAAGTGACACGCACAGGTGTAAAAAAAACTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGACTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCtat                                                                                                            >  2:1430632/1‑148 (MQ=255)                                            aataaaGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCa                                                                                                          >  2:242602/1‑150 (MQ=255)                                              taaaGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCaaa                                                                                                        >  1:697614/1‑150 (MQ=255)                                                aaGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAAtgtg                                                                                                                        <  2:625012/132‑1 (MQ=255)                                                aaGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAAtgtg                                                                                                                        >  1:625012/1‑132 (MQ=255)                                                aaGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATg                                                                                                      <  1:474543/150‑1 (MQ=255)                                                  gCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAg                                                                                                    <  2:648396/150‑1 (MQ=255)                                                   ccTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAgg                                                                                                   <  2:447955/150‑1 (MQ=255)                                                      aTGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACg                                                                                                >  2:315362/1‑150 (MQ=255)                                                        ggTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGtt                                                                                              >  1:1254435/1‑150 (MQ=255)                                                         gTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTc                                                                                             >  1:1282377/1‑150 (MQ=255)                                                         gTGAAGCTAATCGTAATATACAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTc                                                                                             <  2:1449133/150‑1 (MQ=255)                                                          tGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCa                                                                                            >  1:572459/1‑150 (MQ=255)                                                             aGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGa                                                                                         >  2:1288194/1‑150 (MQ=255)                                                              gCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCTAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGAt                                                                                        >  1:402424/1‑150 (MQ=255)                                                                   tCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAg                                                                                   <  2:834414/150‑1 (MQ=255)                                                                     gTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTg                                                                                 <  2:942803/150‑1 (MQ=255)                                                                     gTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTg                                                                                 <  2:929132/150‑1 (MQ=255)                                                                     gTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTg                                                                                 >  1:87787/1‑150 (MQ=255)                                                                      ttATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGa                                                                                <  2:434866/150‑1 (MQ=255)                                                                          ataGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCt                                                                            <  2:138202/150‑1 (MQ=255)                                                                           taGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCtt                                                                           >  2:877449/1‑150 (MQ=255)                                                                            aGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTa                                                                          >  1:1202122/1‑150 (MQ=255)                                                                            aGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGAAGTTCAGGATTTAAGTGACCCTTa                                                                          >  2:807447/1‑150 (MQ=255)                                                                             gAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTAt                                                                         >  1:894413/1‑150 (MQ=255)                                                                              aaCAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTAtt                                                                        >  2:1283656/1‑150 (MQ=255)                                                                               aCAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTc                                                                       <  1:940794/150‑1 (MQ=255)                                                                                 aaGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTg                                                                     <  1:1283656/150‑1 (MQ=255)                                                                                  aGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGa                                                                    <  2:879751/150‑1 (MQ=255)                                                                                    tGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAAt                                                                  <  1:414160/150‑1 (MQ=255)                                                                                     gCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATc                                                                 >  2:693765/1‑150 (MQ=255)                                                                                     gCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCGGAATc                                                                 >  2:1497255/1‑150 (MQ=255)                                                                                       cACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAg                                                               <  1:1293537/150‑1 (MQ=255)                                                                                       cACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAg                                                               >  2:1339934/1‑150 (MQ=255)                                                                                           cacaGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATg                                                           >  2:222989/1‑150 (MQ=255)                                                                                               ggTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAgg                                                                                          <  2:168329/115‑1 (MQ=255)                                                                                               ggTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAgg                                                                                          >  1:168329/1‑115 (MQ=255)                                                                                               ggTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGctc                                                       >  2:1021853/1‑150 (MQ=255)                                                                                               ggTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGctc                                                       >  1:325171/1‑150 (MQ=255)                                                                                               ggTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGctc                                                       >  2:640161/1‑150 (MQ=255)                                                                                                gtgtAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGctct                                                      <  1:693765/150‑1 (MQ=255)                                                                                                    aaaaaaaCCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATg                                                  <  1:1069118/150‑1 (MQ=255)                                                                                                     aaaaaaCCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGt                                                 >  1:1042043/1‑150 (MQ=255)                                                                                                     aaaaaaCCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGt                                                 <  2:1393885/150‑1 (MQ=255)                                                                                                     aaaaaaCCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGt                                                 <  2:1399509/150‑1 (MQ=255)                                                                                                            cTGATACCGAACAACTTAACTTGCATAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGACGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCa                                          <  2:421224/150‑1 (MQ=255)                                                                                                               aaaCCGCACAACCTAACCTGCTTAAAAAATCCCACCGCCGCCGGCCG‑GCTTTTACGGCCAAACAAAGGGAAATAAACCATTCAAAAGCCGCCCGTCACGACTTAACTGCCCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATAc                                       <  2:832820/148‑1 (MQ=255)                                                                                                                tACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGGCGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACa                                      >  1:368706/1‑150 (MQ=255)                                                                                                                 aCCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAg                                     <  1:202678/150‑1 (MQ=255)                                                                                                                   cGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGAc                                   <  1:812202/150‑1 (MQ=255)                                                                                                                    gAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGAcc                                  >  1:1328807/1‑150 (MQ=255)                                                                                                                         acttaacttGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAc                             <  1:284441/150‑1 (MQ=255)                                                                                                                          cttaacttGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcg                            >  1:1418546/1‑150 (MQ=255)                                                                                                                          cttaacttGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcg                            >  1:133857/1‑150 (MQ=255)                                                                                                                            taacttGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcgcg                          <  1:1037442/150‑1 (MQ=255)                                                                                                                            taacttGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcgcg                          <  1:1024636/150‑1 (MQ=255)                                                                                                                               cttGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAAc                       <  1:164579/150‑1 (MQ=255)                                                                                                                                 tGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTc                     <  1:1164727/150‑1 (MQ=255)                                                                                                                                  gCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCg                    <  1:452460/150‑1 (MQ=255)                                                                                                                                   tccctaaaaTC‑CAGCCAGGCTCGCTGTGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATAc                                       <  1:42346/125‑1 (MQ=38)                                                                                                                                   tccctaaaaTC‑CAGCCAGGCTCGCTGTGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATAc                                       >  2:42346/6‑130 (MQ=38)                                                                                                                                   cTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTTGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGc                   >  2:1358145/1‑150 (MQ=255)                                                                                                                                   cTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGc                   >  2:530605/1‑150 (MQ=255)                                                                                                                                   cTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGc                   <  1:594461/150‑1 (MQ=255)                                                                                                                                     tAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCa                 <  1:877449/150‑1 (MQ=255)                                                                                                                                     tAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCa                 >  1:1274027/1‑150 (MQ=255)                                                                                                                                     cctaaaaTC‑CAGCCAGGCTCGCTGTGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCa                 >  2:250607/4‑150 (MQ=255)                                                                                                                                     cctaaaaTC‑CAGCCAGGCTCGCTGTGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCa                 >  1:409505/4‑150 (MQ=255)                                                                                                                                     cctaaaaTC‑CAGCCAGGCTCGCTGTGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCa                 >  2:256017/4‑150 (MQ=255)                                                                                                                                      aaaaaaTCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAg                <  1:1021853/150‑1 (MQ=255)                                                                                                                                      aaaaaaTCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAg                >  1:353972/1‑150 (MQ=255)                                                                                                                                      aaaaaaTCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAg                >  1:348445/1‑150 (MQ=255)                                                                                                                                       aaaaaTCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGt               <  2:894413/150‑1 (MQ=255)                                                                                                                                         aaaTCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGAAGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTg             <  1:807447/150‑1 (MQ=255)                                                                                                                                             cacaGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGc         <  2:1274027/150‑1 (MQ=255)                                                                                                                                                   gAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTATAcc   <  2:1202122/150‑1 (MQ=255)                                                                                                                                                    aGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTATACCg  <  2:1282377/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     ATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTG‑GATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTATACCG  >  NC_000913/1962121‑1962417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑