Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,015,703 T→C 6.0% L275S (TTG→TCG)  fliG → flagellar motor switch protein FliG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,015,7030TC 6.0% 304.0 / 6.5 99L275S (TTG→TCG) fliGflagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base T (52/41);  new base C (3/3);  total (55/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAATTGGCA  >  NC_000913/2015558‑2015852
                                                                                                                                                 |                                                                                                                                                     
cAGAAACTCATCGACGAGATGGTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGGCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgcgc                                                                                                                                                   <  2:1048054/150‑1 (MQ=255)
  gAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCag                                                                                                                                                 <  1:500379/150‑2 (MQ=255)
    aaaTCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGGCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCGGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCagtg                                                                                                                                               <  1:951119/150‑4 (MQ=255)
       tcatcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCagtggct                                                                                                                                            <  1:884183/150‑7 (MQ=255)
            gacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAg                                                                                                                                       <  1:1045168/150‑1 (MQ=255)
            gacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAg                                                                                                                                       >  1:937420/1‑150 (MQ=255)
            gacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAg                                                                                                                                       <  1:817743/150‑1 (MQ=255)
                agaTGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTg                                                                                                                                   >  2:298954/1‑150 (MQ=255)
                   tGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccg                                                                                                                                >  1:553344/1‑150 (MQ=255)
                    gTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccgc                                                                                                                               >  1:443057/1‑150 (MQ=255)
                     ttCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccgcc                                                                                                                              <  2:1288658/150‑1 (MQ=255)
                      tCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGccgccg                                                                                                                             <  2:1340242/150‑1 (MQ=255)
                          gTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGatat                                                                                                                         >  2:216373/1‑150 (MQ=255)
                          gTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGatat                                                                                                                         >  2:2919/1‑150 (MQ=255)
                          gTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGCGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGatat                                                                                                                         >  2:1514978/1‑150 (MQ=255)
                            tCGAGAATCTGGTGGATGTCGATGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTc                                                                                                                       >  1:684799/1‑150 (MQ=255)
                               agaATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTgc                                                                                                                    <  1:10181/150‑1 (MQ=255)
                                 aaTCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTgcgc                                                                                                                  <  2:399635/150‑1 (MQ=255)
                                   tCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGcga                                                                                                                >  2:1534548/1‑150 (MQ=255)
                                    cTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCTCTGATAGTAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGctac                                                                                                               <  2:2912/150‑1 (MQ=255)
                                    cTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCATGCCGCCGATATTCTGCGcgac                                                                                                               <  1:945336/150‑1 (MQ=255)
                                      ggtggATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGcgacga                                                                                                             >  1:1101495/1‑150 (MQ=255)
                                            tGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGc                                                                                                       <  1:146825/150‑1 (MQ=255)
                                            tGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGc                                                                                                       >  2:418567/1‑150 (MQ=255)
                                             gTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGcc                                                                                                      >  2:1005962/1‑150 (MQ=255)
                                             gTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGcc                                                                                                      <  2:553344/150‑1 (MQ=255)
                                               cgacgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCaa                                                                                                    >  1:889174/1‑150 (MQ=255)
                                               cgacgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCaa                                                                                                    >  2:651892/1‑150 (MQ=255)
                                               ccacgaTCGCAGCAATCAGCCTCTCTTCCAGGAACTCGATTCCGAATCGCTGTTGATCCCCCTCAAAGCAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAAAATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCaa                                                                                                    >  2:310861/3‑150 (MQ=255)
                                                gacgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAAc                                                                                                   >  1:1408043/1‑150 (MQ=255)
                                                   gaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGt                                                                                                >  2:47816/1‑150 (MQ=255)
                                                       gcagcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTc                                                                                            >  1:1245231/1‑150 (MQ=255)
                                                         agcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCg                                                                                          <  1:587702/150‑1 (MQ=255)
                                                           caTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGt                                                                                        >  1:1493993/1‑150 (MQ=255)
                                                            aTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTg                                                                                       <  1:2919/150‑1 (MQ=255)
                                                              tCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCg                                                                                     <  1:651892/150‑1 (MQ=255)
                                                              tCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCg                                                                                     <  1:390664/150‑1 (MQ=255)
                                                                aGCGTCTGTTGCAGGTAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTc                                                                                   <  1:167568/150‑1 (MQ=255)
                                                                aGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTc                                                                                   <  1:216373/150‑1 (MQ=255)
                                                                 gCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCt                                                                                  >  2:1340110/1‑150 (MQ=255)
                                                                    tCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTc                                                                               >  2:124633/1‑150 (MQ=255)
                                                                         tGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAgg                                                                          >  1:1197079/1‑150 (MQ=255)
                                                                           cAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTg                                                                        <  1:1436167/150‑1 (MQ=255)
                                                                            aGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTgg                                                                       <  2:1358841/150‑1 (MQ=255)
                                                                               aaGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGaaa                                                                    <  2:703765/150‑1 (MQ=255)
                                                                                 gTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAAc                                                                  >  1:1201849/1‑150 (MQ=255)
                                                                                 gTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAAc                                                                  <  2:873361/150‑1 (MQ=255)
                                                                                  tGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACg                                                                 <  2:850997/150‑1 (MQ=255)
                                                                                  tGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACg                                                                 <  1:940514/150‑1 (MQ=255)
                                                                                     aTTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAAc                                                              >  1:72070/1‑150 (MQ=255)
                                                                                       tCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAg                                                            <  2:1312721/150‑1 (MQ=255)
                                                                                       tCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGAGTCTGTCGCAGGTGGAAAACGAACAg                                                            <  2:1307372/150‑1 (MQ=255)
                                                                                          gAATCGCTGTTGCTCGCGCTGAAAGTAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCTATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGGGGAAAACGAACAGaaa                                                         <  2:1377194/150‑1 (MQ=255)
                                                                                           aaTCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAg                                                        <  2:929650/150‑1 (MQ=255)
                                                                                             tCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTTGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCg                                                      <  2:684799/150‑1 (MQ=255)
                                                                                              cGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGa                                                     <  1:129695/150‑1 (MQ=255)
                                                                                               gCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGAt                                                    >  1:1030088/1‑150 (MQ=255)
                                                                                                cTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGAtt                                                   <  2:1152717/150‑1 (MQ=255)
                                                                                                   ttGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctg                                                >  2:1514877/1‑150 (MQ=255)
                                                                                                      aTCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCCTCGTCCGGTGATTCTGACGCAAGTCGAAAACGAACAGAAAAAGAttatccta                                             >  2:1147149/1‑144 (MQ=255)
                                                                                                         gcgcTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGAtt                                          <  1:432896/150‑1 (MQ=255)
                                                                                                            cTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATtgtg                                       >  1:777597/1‑150 (MQ=255)
                                                                                                             tGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACTGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTgc                                      >  1:136147/1‑150 (MQ=255)
                                                                                                              gAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTgcg                                     >  1:370488/1‑150 (MQ=255)
                                                                                                               aaaGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTgcgc                                    <  2:72070/150‑1 (MQ=255)
                                                                                                                 aGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGcgccg                                  >  2:1221628/1‑150 (MQ=255)
                                                                                                                  ggAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGcgccgc                                 >  1:263310/1‑150 (MQ=255)
                                                                                                                   gAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCgccgcc                                >  1:1192212/1‑150 (MQ=255)
                                                                                                                   gAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCgccgcc                                >  2:589185/1‑150 (MQ=255)
                                                                                                                       cGATCATCCACTGCGGGTTAAATTCTTGCGCAATATGTCGCAGCGGGGCGGCGTTATTTTGGGCGTCGTTCTCGCCAACCGTGGTCCGGTGGGGCTGTCGCATGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGc                            <  2:443057/150‑1 (MQ=255)
                                                                                                                         agcagcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCg                          <  1:1221628/150‑1 (MQ=255)
                                                                                                                           gacatattGCGCAAGAATTTCTCGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGaa                        >  1:476468/8‑150 (MQ=255)
                                                                                                                           cagcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCACCGTGCCGCCGATATTCTGCGCGCCGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGAGGACAACCAACAGAAAGCGAGTCTGCTGATTGTGCGCCGCCTTGCCGaa                        >  1:837909/1‑150 (MQ=255)
                                                                                                                            agcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGaaa                       >  1:967454/1‑150 (MQ=255)
                                                                                                                            agcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGaaa                       >  2:498134/1‑150 (MQ=255)
                                                                                                                            agcCACTGCGCGAGAAATTCTTGCGCAATATGTAGCAGCGTGCGGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTTTGCGCCGCCTTGACGaaa                       >  2:498131/1‑150 (MQ=255)
                                                                                                                             gcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAAc                      >  1:292853/1‑150 (MQ=255)
                                                                                                                             gcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAAc                      >  2:1169401/1‑150 (MQ=255)
                                                                                                                             gcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAAAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAAc                      >  2:420414/1‑150 (MQ=255)
                                                                                                                               cACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGc                            >  2:68164/1‑142 (MQ=255)
                                                                                                                               cACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGc                            <  1:68164/142‑1 (MQ=255)
                                                                                                                                attGCGCAAGAATTTCTCGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTgg                   >  1:634084/3‑150 (MQ=255)
                                                                                                                                attGCGCAAGAATTTCTCGCGCAATATCTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTCATTGTGCGCCGCCTTGCCGAAACTgg                   >  2:1406676/3‑150 (MQ=255)
                                                                                                                                 cTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGc                  >  1:1084013/1‑150 (MQ=255)
                                                                                                                                  tGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCg                 <  1:1534548/150‑1 (MQ=255)
                                                                                                                                  tGCGCCAGAAATTATTAAGCAATATGTAGCAGCGTGCCGCAGATAATCAGCCCGACGATCTAGCCACCCATGGTCCTGTGCGTCTGACGCAGGTTGAAAACGAACAGAAAGCAAATCTGATGATTGTACGCCGCCTTGCCGAAACTGGCg                 >  2:1172266/1‑150 (MQ=255)
                                                                                                                                   gcgcGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCga                >  2:721376/1‑150 (MQ=255)
                                                                                                                                    cgcgAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCgag               <  1:298954/150‑1 (MQ=255)
                                                                                                                                      cgAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGAt             >  1:1070960/1‑150 (MQ=255)
                                                                                                                                      cgAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGAt             <  1:518796/150‑1 (MQ=255)
                                                                                                                                      cgAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGAt             <  2:263310/150‑1 (MQ=255)
                                                                                                                                       gagaAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATg            >  2:1516395/1‑150 (MQ=255)
                                                                                                                                        agaAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATgg           >  1:372098/1‑150 (MQ=255)
                                                                                                                                        agaAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATgg           <  1:1340110/150‑1 (MQ=255)
                                                                                                                                         gaAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGt          >  2:853206/1‑150 (MQ=255)
                                                                                                                                             ttCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAAtt      <  2:1466303/150‑1 (MQ=255)
                                                                                                                                              tCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAATTg     >  1:672496/1‑150 (MQ=255)
                                                                                                                                               cTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAATTgg    >  1:1069445/1‑150 (MQ=255)
                                                                                                                                                 tGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAATTGGCa  >  1:448727/1‑150 (MQ=255)
                                                                                                                                                 tGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAATTGGCa  >  1:1051206/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAATTGGCA  >  NC_000913/2015558‑2015852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑