BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,338,410	C→G	5.1%	L337F (TTG→TTC) ‡	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,410	0	C	G	5.1%	333.0 / 6.6	99	L337F (TTG→TTC) ‡	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand): ref base C (40/54); new base G (3/2); total (43/56)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 101 total aligned reads displayed.

AGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCC  >  NC_000913/2338261‑2338559

aGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑Gc                                                                                                                                                       <  1:472142/150‑1 (MQ=255)

  cgcCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCaa                                                                                                                                                     <  2:1380547/150‑1 (MQ=255)

    ccACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATg                                                                                                                                                   <  2:617374/150‑1 (MQ=255)

     cACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGc                                                                                                                                                  <  1:830037/150‑1 (MQ=255)

        ggCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGccac                                                                                                                                               >  2:1390253/1‑150 (MQ=255)

        ggCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGccac                                                                                                                                               <  2:508198/150‑1 (MQ=255)

         gCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGccacc                                                                                                                                              >  1:1352663/1‑150 (MQ=255)

           tAATGCTTCTATGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACTCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCAt                                                                                                                                            <  2:1425435/150‑1 (MQ=255)

            aaTGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATg                                                                                                                                           <  2:308021/150‑1 (MQ=255)

            aaTGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATg                                                                                                                                           <  2:32764/150‑1 (MQ=255)

             aTGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGt                                                                                                                                          <  1:1466408/150‑1 (MQ=255)

              tGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAGCAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGtt                                                                                                                                         <  1:424286/150‑1 (MQ=255)

              tGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGtt                                                                                                                                         <  2:197223/150‑1 (MQ=255)

               gCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GGCATTGCACCATggtc                                                                                                                                        <  2:59846/150‑4 (MQ=255)

                  tCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATa                                                                                                                                     >  1:1185791/1‑150 (MQ=255)

                   cAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGAACATGGT‑GGCATTGCACCATggtcagcc                                                                                                                                    <  1:459695/150‑8 (MQ=255)

                    aaGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATAcc                                                                                                                                   >  1:493496/1‑150 (MQ=255)

                      ggATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCga                                                                                                                                 >  1:986900/1‑150 (MQ=255)

                       gATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 <  1:941179/133‑1 (MQ=255)

                       gATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 >  2:941179/1‑133 (MQ=255)

                         tatGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCgaaag                                                                                                                              >  2:564577/1‑150 (MQ=255)

                         tatGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCgaaag                                                                                                                              <  2:720953/150‑1 (MQ=255)

                            gAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATAcc                                                                                                                                   <  1:1003125/142‑1 (MQ=255)

                            gAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATAcc                                                                                                                                   >  2:1003125/1‑142 (MQ=255)

                              gCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAAcc                                                                                                                         <  2:331555/150‑1 (MQ=255)

                              gCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAAcc                                                                                                                         >  1:90884/1‑150 (MQ=255)

                              gCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAAcc                                                                                                                         <  1:820330/150‑1 (MQ=255)

                                 cGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGc                                                                                                                      >  1:1287108/1‑150 (MQ=255)

                                 cGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGc                                                                                                                      >  1:1114731/1‑150 (MQ=255)

                                  gATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCa                                                                                                                     <  1:166665/150‑1 (MQ=255)

                                      gcgAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTg                                                                                                                 >  2:82715/1‑150 (MQ=255)

                                             ttACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTgg                                                                                                          >  1:856330/1‑150 (MQ=255)

                                              tACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTggg                                                                                                         >  2:46064/1‑150 (MQ=255)

                                                cGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAg                                                                                                       <  2:260277/150‑1 (MQ=255)

                                                 gCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGt                                                                                                      <  1:919677/150‑1 (MQ=255)

                                                       cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 >  2:683513/1‑101 (MQ=255)

                                                       cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 <  1:683513/101‑1 (MQ=255)

                                                       cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgt                                                                                                >  2:449429/1‑150 (MQ=255)

                                                       cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgt                                                                                                <  2:980565/150‑1 (MQ=255)

                                                       cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCCGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGTTAGTAGAGgt                                                                                                <  2:822695/150‑1 (MQ=255)

                                                          aaaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgttgt                                                                                             >  2:817336/1‑150 (MQ=255)

                                                          aaaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgttgt                                                                                             <  1:279025/150‑1 (MQ=255)

                                                           aaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgttgtt                                                                                            <  2:1185791/150‑1 (MQ=255)

                                                            aTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGttgttg                                                                                           <  2:90884/150‑1 (MQ=255)

                                                             tAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGa                                                                                          <  1:1116599/150‑1 (MQ=255)

                                                                  cgacgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGcaca                                                                                     >  1:494884/1‑150 (MQ=255)

                                                                   gacgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACaa                                                                                    <  1:1390253/150‑1 (MQ=255)

                                                                    acgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAAc                                                                                   <  1:420932/150‑1 (MQ=255)

                                                                     cgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACt                                                                                  <  1:1452901/150‑1 (MQ=255)

                                                                       ggTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTc                                                                                <  1:596142/150‑1 (MQ=255)

                                                                         tCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTGCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCac                                                                              <  2:338549/150‑1 (MQ=255)

                                                                          ttgttcgtCACCGCCGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCacc                                                                             >  2:354292/8‑150 (MQ=255)

                                                                           accacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCaccg                                                                            >  1:1257069/1‑150 (MQ=255)

                                                                            ccacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCaccga                                                                           >  1:368032/1‑150 (MQ=255)

                                                                              acTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCaccgacc                                                                         >  2:333698/1‑150 (MQ=255)

                                                                                  cACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCAt                                                                     >  2:754494/1‑150 (MQ=255)

                                                                                     ggcggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAACCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGCCCGCATcgc                                                                  >  2:253102/1‑150 (MQ=255)

                                                                                        ggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGtt                                                               <  1:796175/150‑1 (MQ=255)

                                                                                        ggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGtt                                                               <  2:1257069/150‑1 (MQ=255)

                                                                                        ggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGtt                                                               >  2:119570/1‑150 (MQ=255)

                                                                                         gTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGttt                                                              <  1:221474/150‑1 (MQ=255)

                                                                                          tGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTc                                                             <  1:82715/150‑1 (MQ=255)

                                                                                           gACGAACAAACGCCGCGATGATGTCTTTCGGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCa                                                            <  1:210851/150‑1 (MQ=255)

                                                                                              gAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCCATGCCACCATGTTCATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAAGTTCACCGACCGCCTCGCGTTTCACtt                                                         >  2:980267/1‑150 (MQ=255)

                                                                                                  aaaCGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAAt                                                     <  2:1287108/150‑1 (MQ=255)

                                                                                                   aaCGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATc                                                    >  1:596077/1‑150 (MQ=255)

                                                                                                          cgATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATgc                                             <  2:2399/150‑1 (MQ=255)

                                                                                                                 ggcTTTCAGGTTCATGATCTTCGGCTGAGCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGGCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGAGCGCATCGCGTTTCACTTCAATCACGATGCGCATTcc                                      <  2:1276324/148‑1 (MQ=255)

                                                                                                                  tCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCg                                     <  1:484237/150‑1 (MQ=255)

                                                                                                                   cTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                 <  1:644196/137‑1 (MQ=255)

                                                                                                                   cTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                 >  2:644196/1‑137 (MQ=255)

                                                                                                                     ttCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCt                                  <  2:918324/150‑1 (MQ=255)

                                                                                                                     ttCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCt                                  >  2:112652/1‑150 (MQ=255)

                                                                                                                      tCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCtt                                 >  1:498704/1‑150 (MQ=255)

                                                                                                                       cAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATgcg                                            <  1:1243599/138‑1 (MQ=255)

                                                                                                                       cAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATgcg                                            >  2:1243599/1‑138 (MQ=255)

                                                                                                                        aGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTg                               <  1:327853/150‑1 (MQ=255)

                                                                                                                          gTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTc                             <  2:1187061/150‑1 (MQ=255)

                                                                                                                           ttCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                            <  1:817336/150‑1 (MQ=255)

                                                                                                                           ttCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                            <  1:646741/150‑1 (MQ=255)

                                                                                                                           ttCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGGTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                            <  2:45648/150‑1 (MQ=255)

                                                                                                                              aTGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGAc                         <  1:449429/150‑1 (MQ=255)

                                                                                                                               taaTCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        >  1:1059247/3‑150 (MQ=255)

                                                                                                                               tGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        >  1:461158/1‑150 (MQ=255)

                                                                                                                               tGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        <  1:46064/150‑1 (MQ=255)

                                                                                                                               tGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        >  1:949203/1‑150 (MQ=255)

                                                                                                                                gATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTc                       >  2:1513888/1‑150 (MQ=255)

                                                                                                                                 aTCTTCGGCTGACCATGGG‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCg                      <  2:1352663/150‑1 (MQ=255)

                                                                                                                                  tCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGt                     <  1:548715/150‑1 (MQ=255)

                                                                                                                                    ttCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCa                   >  2:313530/1‑150 (MQ=255)

                                                                                                                                    ttCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCa                   <  1:921754/150‑1 (MQ=255)

                                                                                                                                        gCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCa               >  2:226083/1‑150 (MQ=255)

                                                                                                                                           caacATGGT‑GGCATTGCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGCCCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcg            >  2:272284/4‑150 (MQ=255)

                                                                                                                                           caacATGGT‑GGCATTGCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcg            >  1:333981/4‑150 (MQ=255)

                                                                                                                                            aacATGGTGGCATTGCC‑CCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgc           >  1:259527/3‑150 (MQ=255)

                                                                                                                                            aacATGGT‑GGCATTGCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgc           >  2:933179/3‑150 (MQ=255)

                                                                                                                                            aCCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgc           >  2:613590/1‑150 (MQ=255)

                                                                                                                                                   t‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATg    >  1:1011957/1‑150 (MQ=255)

                                                                                                                                                     gCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGc   <  2:368032/150‑1 (MQ=255)

                                                                                                                                                      cAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGcc  >  2:1063104/1‑150 (MQ=255)

AGCGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCC  >  NC_000913/2338261‑2338559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`