Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,338,411 A→C 5.7% L337W (TTG→TGG) ‡ gyrA ← DNA gyrase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,338,4110AC 5.7% 320.0 / 5.9 99L337W (TTG→TGG) ‡gyrADNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base A (40/53);  new base C (4/2);  total (44/55)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCC  >  NC_000913/2338263‑2338559
                                                                                                                                                     |                                                                                                                                                    
cgcCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCaa                                                                                                                                                     <  2:1380547/150‑1 (MQ=255)
  ccACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATg                                                                                                                                                   <  2:617374/150‑1 (MQ=255)
   cACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGc                                                                                                                                                  <  1:830037/150‑1 (MQ=255)
      ggCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGccac                                                                                                                                               >  2:1390253/1‑150 (MQ=255)
      ggCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGccac                                                                                                                                               <  2:508198/150‑1 (MQ=255)
       gCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGccacc                                                                                                                                              >  1:1352663/1‑150 (MQ=255)
         tAATGCTTCTATGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACTCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCAt                                                                                                                                            <  2:1425435/150‑1 (MQ=255)
          aaTGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATg                                                                                                                                           <  2:32764/150‑1 (MQ=255)
          aaTGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATg                                                                                                                                           <  2:308021/150‑1 (MQ=255)
           aTGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGt                                                                                                                                          <  1:1466408/150‑1 (MQ=255)
            tGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAGCAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGtt                                                                                                                                         <  1:424286/150‑1 (MQ=255)
            tGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGtt                                                                                                                                         <  2:197223/150‑1 (MQ=255)
             gCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GGCATTGCACCATggtc                                                                                                                                        <  2:59846/150‑4 (MQ=255)
                tCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATa                                                                                                                                     >  1:1185791/1‑150 (MQ=255)
                 cAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGAACATGGT‑GGCATTGCACCATggtcagcc                                                                                                                                    <  1:459695/150‑8 (MQ=255)
                  aaGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATAcc                                                                                                                                   >  1:493496/1‑150 (MQ=255)
                    ggATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCga                                                                                                                                 >  1:986900/1‑150 (MQ=255)
                     gATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 <  1:941179/133‑1 (MQ=255)
                     gATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 >  2:941179/1‑133 (MQ=255)
                       tatGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCgaaag                                                                                                                              >  2:564577/1‑150 (MQ=255)
                       tatGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCgaaag                                                                                                                              <  2:720953/150‑1 (MQ=255)
                          gAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATAcc                                                                                                                                   <  1:1003125/142‑1 (MQ=255)
                          gAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATAcc                                                                                                                                   >  2:1003125/1‑142 (MQ=255)
                            gCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAAcc                                                                                                                         <  2:331555/150‑1 (MQ=255)
                            gCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAAcc                                                                                                                         >  1:90884/1‑150 (MQ=255)
                            gCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAAcc                                                                                                                         <  1:820330/150‑1 (MQ=255)
                               cGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGc                                                                                                                      >  1:1287108/1‑150 (MQ=255)
                               cGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGc                                                                                                                      >  1:1114731/1‑150 (MQ=255)
                                gATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCa                                                                                                                     <  1:166665/150‑1 (MQ=255)
                                    gcgAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTg                                                                                                                 >  2:82715/1‑150 (MQ=255)
                                           ttACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTgg                                                                                                          >  1:856330/1‑150 (MQ=255)
                                            tACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTggg                                                                                                         >  2:46064/1‑150 (MQ=255)
                                              cGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAg                                                                                                       <  2:260277/150‑1 (MQ=255)
                                               gCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGt                                                                                                      <  1:919677/150‑1 (MQ=255)
                                                     cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 >  2:683513/1‑101 (MQ=255)
                                                     cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGcc                                                                                                                                                 <  1:683513/101‑1 (MQ=255)
                                                     cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgt                                                                                                >  2:449429/1‑150 (MQ=255)
                                                     cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgt                                                                                                <  2:980565/150‑1 (MQ=255)
                                                     cGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCCGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGTTAGTAGAGgt                                                                                                <  2:822695/150‑1 (MQ=255)
                                                        aaaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgttgt                                                                                             >  2:817336/1‑150 (MQ=255)
                                                        aaaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgttgt                                                                                             <  1:279025/150‑1 (MQ=255)
                                                         aaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGgttgtt                                                                                            <  2:1185791/150‑1 (MQ=255)
                                                          aTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGttgttg                                                                                           <  2:90884/150‑1 (MQ=255)
                                                           tAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGa                                                                                          <  1:1116599/150‑1 (MQ=255)
                                                                cgacgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGcaca                                                                                     >  1:494884/1‑150 (MQ=255)
                                                                 gacgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACaa                                                                                    <  1:1390253/150‑1 (MQ=255)
                                                                  acgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAAc                                                                                   <  1:420932/150‑1 (MQ=255)
                                                                   cgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACt                                                                                  <  1:1452901/150‑1 (MQ=255)
                                                                     ggTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTc                                                                                <  1:596142/150‑1 (MQ=255)
                                                                       tCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTGCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCac                                                                              <  2:338549/150‑1 (MQ=255)
                                                                        ttgttcgtCACCGCCGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCacc                                                                             >  2:354292/8‑150 (MQ=255)
                                                                         accacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCaccg                                                                            >  1:1257069/1‑150 (MQ=255)
                                                                          ccacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCaccga                                                                           >  1:368032/1‑150 (MQ=255)
                                                                            acTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCaccgacc                                                                         >  2:333698/1‑150 (MQ=255)
                                                                                cACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCAt                                                                     >  2:754494/1‑150 (MQ=255)
                                                                                   ggcggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAACCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGCCCGCATcgc                                                                  >  2:253102/1‑150 (MQ=255)
                                                                                      ggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGtt                                                               >  2:119570/1‑150 (MQ=255)
                                                                                      ggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGtt                                                               <  2:1257069/150‑1 (MQ=255)
                                                                                      ggTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGtt                                                               <  1:796175/150‑1 (MQ=255)
                                                                                       gTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGttt                                                              <  1:221474/150‑1 (MQ=255)
                                                                                        tGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTc                                                             <  1:82715/150‑1 (MQ=255)
                                                                                         gACGAACAAACGCCGCGATGATGTCTTTCGGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCa                                                            <  1:210851/150‑1 (MQ=255)
                                                                                            gAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCCATGCCACCATGTTCATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAAGTTCACCGACCGCCTCGCGTTTCACtt                                                         >  2:980267/1‑150 (MQ=255)
                                                                                             aaCAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTc                                                        <  2:986900/150‑1 (MQ=255)
                                                                                                aaaCGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAAt                                                     <  2:1287108/150‑1 (MQ=255)
                                                                                                 aaCGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATc                                                    >  1:596077/1‑150 (MQ=255)
                                                                                                        cgATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATgc                                             <  2:2399/150‑1 (MQ=255)
                                                                                                               ggcTTTCAGGTTCATGATCTTCGGCTGAGCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGGCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGAGCGCATCGCGTTTCACTTCAATCACGATGCGCATTcc                                      <  2:1276324/148‑1 (MQ=255)
                                                                                                                tCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCg                                     <  1:484237/150‑1 (MQ=255)
                                                                                                                 cTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                 <  1:644196/137‑1 (MQ=255)
                                                                                                                 cTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                 >  2:644196/1‑137 (MQ=255)
                                                                                                                   ttCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCt                                  >  2:112652/1‑150 (MQ=255)
                                                                                                                   ttCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCt                                  <  2:918324/150‑1 (MQ=255)
                                                                                                                    tCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCtt                                 >  1:498704/1‑150 (MQ=255)
                                                                                                                     cAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATgcg                                            >  2:1243599/1‑138 (MQ=255)
                                                                                                                     cAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATgcg                                            <  1:1243599/138‑1 (MQ=255)
                                                                                                                      aGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTg                               <  1:327853/150‑1 (MQ=255)
                                                                                                                        gTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTc                             <  2:1187061/150‑1 (MQ=255)
                                                                                                                         ttCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                            <  1:646741/150‑1 (MQ=255)
                                                                                                                         ttCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                            <  1:817336/150‑1 (MQ=255)
                                                                                                                         ttCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGGTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                            <  2:45648/150‑1 (MQ=255)
                                                                                                                            aTGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGAc                         <  1:449429/150‑1 (MQ=255)
                                                                                                                             taaTCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        >  1:1059247/3‑150 (MQ=255)
                                                                                                                             tGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        >  1:949203/1‑150 (MQ=255)
                                                                                                                             tGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        >  1:461158/1‑150 (MQ=255)
                                                                                                                             tGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACt                        <  1:46064/150‑1 (MQ=255)
                                                                                                                              gATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTc                       >  2:1513888/1‑150 (MQ=255)
                                                                                                                               aTCTTCGGCTGACCATGGG‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCg                      <  2:1352663/150‑1 (MQ=255)
                                                                                                                                tCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGt                     <  1:548715/150‑1 (MQ=255)
                                                                                                                                  ttCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCa                   <  1:921754/150‑1 (MQ=255)
                                                                                                                                  ttCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCa                   >  2:313530/1‑150 (MQ=255)
                                                                                                                                      gCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCa               >  2:226083/1‑150 (MQ=255)
                                                                                                                                         caacATGGT‑GGCATTGCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGCCCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcg            >  2:272284/4‑150 (MQ=255)
                                                                                                                                         caacATGGT‑GGCATTGCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcg            >  1:333981/4‑150 (MQ=255)
                                                                                                                                          aacATGGTGGCATTGCC‑CCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgc           >  1:259527/3‑150 (MQ=255)
                                                                                                                                          aacATGGT‑GGCATTGCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgc           >  2:933179/3‑150 (MQ=255)
                                                                                                                                          aCCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgc           >  2:613590/1‑150 (MQ=255)
                                                                                                                                                 t‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATg    >  1:1011957/1‑150 (MQ=255)
                                                                                                                                                   gCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGc   <  2:368032/150‑1 (MQ=255)
                                                                                                                                                    cAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGcc  >  2:1063104/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCCACGGCTAATGCTTCAAGGATATGAGCACGATCGCGAGCTTTACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGT‑GCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCC  >  NC_000913/2338263‑2338559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑