Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,965:1 +C 6.3% coding (134/567 nt) yecD → putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,9651.C 6.3% 320.3 / 16.9 79coding (134/567 nt)yecDputative hydrolase
Reads supporting (aligned to +/- strand):  ref base . (34/40);  new base C (3/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

TTTTTCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAAC  >  NC_000913/1950816‑1951109
                                                                                                                                                      |                                                                                                                                                
tttttCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg                                                                                                                                                   >  1:800225/1‑150 (MQ=255)
     cAGGAGTCAATATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGAAGTTAATCGCGCCGGGAAGCTGGAGGCGAAAATTCG‑CGCCa                                                                                                                                             >  1:1551082/1‑150 (MQ=255)
      aGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGC‑GCGAAATTTCGCCGCCAg                                                                                                                                            <  1:1007098/150‑1 (MQ=255)
            cATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGC‑GCGAAATTTCGCCGCCAGcttccc                                                                                                                                      <  1:392334/150‑6 (MQ=255)
                 tGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGAATTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCg                                                                                                                                 >  2:966194/1‑150 (MQ=255)
                   cTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCgtg                                                                                                                               >  2:346657/1‑150 (MQ=255)
                    ttGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCgtgt                                                                                                                              >  1:1614267/1‑150 (MQ=255)
                     tGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGtt                                                                                                                             >  1:1285184/1‑150 (MQ=255)
                       aaCTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTc                                                                                                                           <  1:596606/150‑1 (MQ=255)
                        aCTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCt                                                                                                                          <  2:1293740/150‑1 (MQ=255)
                        aCTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCt                                                                                                                          >  1:624675/1‑150 (MQ=255)
                        aCTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCt                                                                                                                          >  1:1010621/1‑150 (MQ=255)
                         cTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTg                                                                                                                         <  2:1211444/150‑1 (MQ=255)
                          ttAATGCTAAAACCACTGCGCTGGGGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTgg                                                                                                                        <  1:1176725/150‑1 (MQ=255)
                              tGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGTTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTgcg                                                                                                                    <  2:718652/150‑1 (MQ=255)
                              tGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg‑cg                                                                                                                                                >  2:212120/1‑122 (MQ=255)
                              tGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg‑cg                                                                                                                                                <  1:212120/122‑1 (MQ=255)
                               gCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTgcgc                                                                                                                   >  1:1194824/1‑150 (MQ=255)
                                cTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGcgcg                                                                                                                  <  2:991056/150‑1 (MQ=255)
                                 tAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGt                                                                                                                 >  1:336140/1‑150 (MQ=255)
                                 tAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGt                                                                                                                 >  2:391900/1‑150 (MQ=255)
                                  aaaaCCACTGCGCTTGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGtt                                                                                                                <  1:954918/150‑1 (MQ=255)
                                   aaaCCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTg                                                                                                               <  1:1221049/150‑1 (MQ=255)
                                     aCCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGc                                                                                                             <  2:983128/150‑1 (MQ=255)
                                     aCCACTGCGCTGGTGGTGATCGATTTAAAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGAAGAGGTAGTTAATCGCGCCGGGAAGCTGGCTGCGAAATATCG‑CGCCAGCGGTCAGCCCGTGTTACTGGTGCGCGTTGGc                                                                                                             >  2:872880/1‑150 (MQ=255)
                                                ggtggtGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCTCGTTGGCTTGTCTGCCGa                                                                                                  <  2:1596515/150‑1 (MQ=255)
                                                  tggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAtt                                                                                                <  1:1005985/150‑1 (MQ=255)
                                                      gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                                                                            <  2:1118186/150‑1 (MQ=255)
                                                          gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGaa                                                                                        <  1:223051/150‑1 (MQ=255)
                                                           aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAg                                                                                       <  1:942247/150‑1 (MQ=255)
                                                             ttACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGATGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCa                                                                                     <  2:64914/150‑1 (MQ=255)
                                                               aCAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAtt                                                                                   <  1:95605/150‑1 (MQ=255)
                                                                   gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaaa                                                                               <  2:603524/150‑1 (MQ=255)
                                                                         aTCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCg                                                                         <  2:1614267/150‑1 (MQ=255)
                                                                           cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGcc                                                                                                    <  1:1453437/121‑1 (MQ=255)
                                                                           cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGcc                                                                                                    >  2:1453437/1‑121 (MQ=255)
                                                                            ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                                                                      >  1:1014354/1‑150 (MQ=255)
                                                                             tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                                                                     <  2:1144666/150‑1 (MQ=255)
                                                                             tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                                                                     >  1:402830/1‑150 (MQ=255)
                                                                              aCCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGtgag                                                                    >  1:565464/1‑147 (MQ=255)
                                                                                            gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg                                                      >  2:246683/1‑150 (MQ=255)
                                                                                              ccACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCt                                                    <  1:117416/150‑1 (MQ=255)
                                                                                                acaTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaa                                                  >  1:500453/1‑150 (MQ=255)
                                                                                                acaTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaa                                                  >  1:1194815/1‑150 (MQ=255)
                                                                                                 caTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaaa                                                 >  2:1091828/1‑150 (MQ=255)
                                                                                                    aCTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgtg                                              <  1:726077/150‑1 (MQ=255)
                                                                                                    aCTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATT‑AAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgtgt                                             <  1:1606227/150‑1 (MQ=255)
                                                                                                     cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                                                                      >  2:859295/1‑125 (MQ=255)
                                                                                                     cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                                                                      <  1:859295/125‑1 (MQ=255)
                                                                                                     cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgtgt                                             >  2:739279/1‑150 (MQ=255)
                                                                                                     cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgtgt                                             >  1:112192/1‑150 (MQ=255)
                                                                                                      tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGtt                                            >  1:80833/1‑150 (MQ=255)
                                                                                                       ggcGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGTCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGGTTCTGGTGCGCGTTGGGTGGTGTGCCGATTACGCTGAAGGATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTg                                           <  2:1551082/148‑1 (MQ=255)
                                                                                                       gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTg                                           <  1:1275246/150‑1 (MQ=255)
                                                                                                        ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGc                                          <  1:806519/150‑1 (MQ=255)
                                                                                                        ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGc                                          <  1:450428/150‑1 (MQ=255)
                                                                                                            tGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGa                                      >  1:1533568/1‑150 (MQ=255)
                                                                                                               ggTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGaaaa                                   >  1:37810/1‑150 (MQ=255)
                                                                                                                  aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtg                                >  2:556500/1‑150 (MQ=255)
                                                                                                                  aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGATGAAGCATTAAAACAGCCGGTCGATGCGACCTCACCGTCTAAAGTGTTGCCCGAAAATtg                                >  1:193283/1‑150 (MQ=255)
                                                                                                                   gTTAATCGCGCCGGGAAGCTGGCGGCGAATTTTCG‑CGCCAGCGGTCAGGCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGGATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                               <  2:340721/150‑1 (MQ=255)
                                                                                                                    ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtggt                              <  1:556500/150‑1 (MQ=255)
                                                                                                                    ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtggt                              <  2:624675/150‑1 (MQ=255)
                                                                                                                       aTCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGc                           <  1:763702/150‑1 (MQ=255)
                                                                                                                       aTCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGc                           >  2:1482225/1‑150 (MQ=255)
                                                                                                                       aTCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGACCGAAAATTGGTGGc                           <  1:346657/150‑1 (MQ=255)
                                                                                                                         cgcgCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCaa                         <  2:272856/150‑1 (MQ=255)
                                                                                                                         cgcgCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCaa                         >  2:141615/1‑150 (MQ=255)
                                                                                                                         cgcgCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCaa                         >  2:1173600/1‑150 (MQ=255)
                                                                                                                             ccGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATc                     >  2:354244/1‑150 (MQ=255)
                                                                                                                              ctgaccgCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGAGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATcc                    >  2:459794/7‑150 (MQ=255)
                                                                                                                                  ccgCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgct                >  1:1271447/3‑150 (MQ=255)
                                                                                                                                   aGCTGGCGGCGAAATTTCG‑CGCCAGCGGTGAGCCCGTCTTTCTGGTGCGCGTTAGCTGGTCTGACGATTAAGCTGAAGCATTAAAACAGCCGGTCGATGCTCACTAACAGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgctg               >  2:858616/1‑150 (MQ=255)
                                                                                                                                    gCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCtgctgc              >  1:157428/1‑150 (MQ=255)
                                                                                                                                      tGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCAt            <  1:141615/150‑1 (MQ=255)
                                                                                                                                         cggcgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAg         <  1:537489/150‑1 (MQ=255)
                                                                                                                                         cggcgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAg         <  2:1010621/150‑1 (MQ=255)
                                                                                                                                            cgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTg      <  1:1091828/150‑1 (MQ=255)
                                                                                                                                            cgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTg      >  1:296640/1‑150 (MQ=255)
                                                                                                                                              aaaTTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCa    <  2:1285184/150‑1 (MQ=255)
                                                                                                                                                aTTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAAc  <  1:4370/150‑1 (MQ=255)
                                                                                                                                                aTTTCG‑CGCCAGCGGTCAGCCCGTGGTACTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCatc  >  2:1171573/1‑148 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
TTTTTCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAAC  >  NC_000913/1950816‑1951109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑