BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,926,891	G→C	10.6%	S650* (TCA→TGA)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,891	0	G	C	10.6%	381.2 / 30.1	123	S650* (TCA→TGA)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand): ref base G (53/57); new base C (5/8); total (58/65)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 124 total aligned reads displayed.

AGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGT  >  NC_000913/1926742‑1927036

aGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTg                                                                                                                                                   <  2:764450/150‑1 (MQ=255)

  cTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAg                                                                                                                                                 <  1:531232/150‑1 (MQ=255)

   tGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                                                                                >  1:1167667/1‑150 (MQ=255)

    gCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAAAGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACAAGATTTACCGCCATGGCCTGAGTc                                                                                                                                               >  2:695992/1‑150 (MQ=255)

      gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                             >  1:500115/1‑150 (MQ=255)

      gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                             <  1:733694/150‑1 (MQ=255)

      gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                             <  1:128550/150‑1 (MQ=255)

      gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                             >  1:1197093/1‑150 (MQ=255)

      gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGCTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                             <  2:213060/150‑1 (MQ=255)

           acacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCg                                                                                                                                        <  2:206919/150‑1 (MQ=255)

           acacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCg                                                                                                                                        <  2:856105/150‑1 (MQ=255)

            cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                                                                       <  2:1412394/150‑1 (MQ=255)

              cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTa                                                                                                                                     <  1:1160/150‑1 (MQ=255)

              cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTa                                                                                                                                     <  2:124300/150‑1 (MQ=255)

                gAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                                                                   >  2:1256301/1‑150 (MQ=255)

                gAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaa                                                                                                                                   <  1:1309246/150‑3 (MQ=255)

                 aaaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag                                                                                                                                  <  2:1010790/150‑1 (MQ=255)

                    cATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatctg                                                                                                                               <  2:1262126/150‑7 (MQ=255)

                     aTTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCa                                                                                                                              >  1:1463334/1‑150 (MQ=255)

                     aTTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATGTACCGACATGGACTCAGGCCATGGCGGTaaatctg                                                                                                                               >  1:1379954/1‑143 (MQ=38)

                      ttATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCaa                                                                                                                             >  1:803855/1‑150 (MQ=255)

                      ttATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCaa                                                                                                                             <  2:1217160/150‑1 (MQ=255)

                       tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                                                            >  2:926781/1‑150 (MQ=255)

                       tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                                                            >  2:921147/1‑150 (MQ=255)

                       tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                                                            >  2:1052007/1‑150 (MQ=255)

                       tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                                                            <  2:1328258/150‑1 (MQ=255)

                        aTCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATa                                                                                                                           <  2:1044574/150‑1 (MQ=255)

                            ggAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAg                                                                                                                       >  1:246892/1‑150 (MQ=255)

                             gAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGa                                                                                                                      >  1:118076/1‑150 (MQ=255)

                                aaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATgg                                                                                                                   <  1:80369/150‑1 (MQ=255)

                                 taccTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGt                                                                                                                  <  1:1033791/147‑1 (MQ=255)

                                 aTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGt                                                                                                                  <  2:1236959/150‑1 (MQ=255)

                                  tACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTc                                                                                                                 >  1:1542034/1‑150 (MQ=255)

                                     ttAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATc                                                                                                              >  1:16917/1‑150 (MQ=255)

                                      tAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCg                                                                                                             >  2:1045351/1‑150 (MQ=255)

                                        gTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGt                                                                                                           >  1:382350/1‑150 (MQ=255)

                                          ccGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGttt                                                                                                         >  1:878244/1‑150 (MQ=255)

                                          ccGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGttt                                                                                                         <  1:1078614/150‑1 (MQ=255)

                                            gCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCTTGTCGGTACAGAGCAATAAAAGATGGTCATCGGtttta                                                                                                       <  1:1068611/150‑2 (MQ=255)

                                            gCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                                                                       >  2:413280/1‑150 (MQ=255)

                                              aGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                                                                                     <  2:500115/150‑1 (MQ=255)

                                               ggCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGc                                                                                                    >  2:1540179/1‑150 (MQ=255)

                                                 cGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTc                                                                                                  <  1:598652/150‑1 (MQ=255)

                                                   tAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgc                                                                                                <  1:74239/150‑1 (MQ=255)

                                                    aGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                                                               >  2:1106299/1‑150 (MQ=255)

                                                          tAATGTTCCCTGCGCCAGCGCGACCAGAGAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAttt                                                                                         <  1:1382458/150‑1 (MQ=255)

                                                            aTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAg                                                                                       >  1:694671/1‑150 (MQ=255)

                                                             tGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                                                                                      <  2:126817/150‑1 (MQ=255)

                                                              gTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                                                                                     >  2:209365/1‑150 (MQ=255)

                                                                   cTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCa                                                                                >  2:1016440/1‑150 (MQ=255)

                                                                    tGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAt                                                                               >  2:411581/1‑150 (MQ=255)

                                                                    tGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAt                                                                               >  1:1598784/1‑150 (MQ=255)

                                                                      cgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAttt                                                                             >  2:69809/1‑150 (MQ=255)

                                                                      cgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAttt                                                                             >  2:152189/1‑150 (MQ=255)

                                                                          aGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                         <  1:1384227/150‑1 (MQ=255)

                                                                           gcgcGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCg                                                                        <  2:1325188/150‑1 (MQ=255)

                                                                            cgcgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCgg                                                                       <  1:1042945/150‑1 (MQ=255)

                                                                             gcgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGt                                                                      <  1:209365/150‑1 (MQ=255)

                                                                                aCCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTcc                                                                   >  2:596808/1‑150 (MQ=255)

                                                                                 ccAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                                                                  <  1:152189/150‑1 (MQ=255)

                                                                                  cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCa                                                                 >  1:792404/1‑150 (MQ=255)

                                                                                   aGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCaa                                                                >  1:681983/1‑150 (MQ=255)

                                                                                    gAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAat                                                               >  2:1144071/1‑150 (MQ=255)

                                                                                    gAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAat                                                               <  1:1530317/150‑1 (MQ=255)

                                                                                       aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt                                                            <  1:166945/150‑1 (MQ=255)

                                                                                       aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt                                                            <  1:596808/150‑1 (MQ=255)

                                                                                       aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt                                                            <  1:584657/150‑1 (MQ=255)

                                                                                          cATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGTTGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCGATTTTGCCGGTTCCCAATTTTGCa                                                         <  2:467917/150‑1 (MQ=255)

                                                                                              ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTg                                                     <  2:1598784/150‑1 (MQ=255)

                                                                                               tCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                                                    <  1:1384418/150‑1 (MQ=255)

                                                                                                     gCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCg                                              <  2:134359/150‑1 (MQ=255)

                                                                                                       tACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTg                                            <  1:251874/150‑1 (MQ=255)

                                                                                                        aCGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGc                                           <  1:1013325/150‑1 (MQ=255)

                                                                                                         cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCa                                          <  1:1081457/150‑1 (MQ=255)

                                                                                                           ccTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCaaa                                        >  2:1585777/1‑150 (MQ=255)

                                                                                                               cGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCgg                                    <  2:382350/150‑1 (MQ=255)

                                                                                                                  aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCg                                 <  2:601060/150‑1 (MQ=255)

                                                                                                                   cGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGt                                >  1:2856/1‑150 (MQ=255)

                                                                                                                       ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                            <  1:264060/150‑1 (MQ=255)

                                                                                                                       ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                            <  1:1284474/150‑1 (MQ=255)

                                                                                                                        tAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCa                           >  2:1579681/1‑150 (MQ=255)

                                                                                                                        tAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTGCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCa                           >  1:1202896/1‑150 (MQ=255)

                                                                                                                           aGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTa                        >  2:617273/1‑150 (MQ=255)

                                                                                                                                  aGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCgg                 >  2:1484411/1‑150 (MQ=255)

                                                                                                                                     tttACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATa              >  2:1277637/1‑150 (MQ=255)

                                                                                                                                     tttACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATa              >  1:772571/1‑150 (MQ=255)

                                                                                                                                      gtACCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATaa             >  2:1439989/2‑150 (MQ=255)

                                                                                                                                       tACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAg            >  2:914066/1‑150 (MQ=255)

                                                                                                                                       tACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAg            <  2:426936/150‑1 (MQ=255)

                                                                                                                                        aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGc           >  2:222142/1‑150 (MQ=255)

                                                                                                                                         ccGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGcc          <  1:1277637/150‑1 (MQ=255)

                                                                                                                                         ccGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAATTTGCCGGTTCCAAATATTGCACCTGAGAAACGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGca          >  1:1581585/1‑149 (MQ=255)

                                                                                                                                         ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGcc          >  1:1240783/1‑150 (MQ=255)

                                                                                                                                           gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtg        <  2:1463334/150‑1 (MQ=255)

                                                                                                                                           gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtg        >  2:1302884/1‑150 (MQ=255)

                                                                                                                                           gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtg        <  1:513189/150‑1 (MQ=255)

                                                                                                                                           gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAACCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtg        >  1:1005393/1‑150 (MQ=255)

                                                                                                                                              aTGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGc     <  1:411581/150‑1 (MQ=255)

                                                                                                                                                 gCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGt  <  1:921147/150‑1 (MQ=255)

                                                                                                                                                 gCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGt  <  1:926781/150‑1 (MQ=255)

AGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGT  >  NC_000913/1926742‑1927036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`