Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,954,996 T→A 5.2% Q671L (CAG→CTG)  torZ ← trimethylamine N‑oxide reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,954,9960TA 5.2% 248.0 / 2.3 77Q671L (CAG→CTG) torZtrimethylamine N‑oxide reductase 2
Reads supporting (aligned to +/- strand):  ref base T (41/32);  new base A (2/2);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACC  >  NC_000913/1954848‑1955142
                                                                                                                                                    |                                                                                                                                                  
cACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTg                                                                                                                                                   >  1:884592/1‑150 (MQ=255)
    aGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAAc                                                                                                                                               <  1:977957/150‑1 (MQ=255)
    aGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAAc                                                                                                                                               <  1:1211164/150‑1 (MQ=255)
    aGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAAc                                                                                                                                               <  1:939340/150‑1 (MQ=255)
      aTCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGCGATTGGTTCACGATCTGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTg                                                                                                                                             <  2:127966/150‑1 (MQ=255)
       tCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGc                                                                                                                                            >  1:969747/1‑150 (MQ=255)
        cGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCt                                                                                                                                           >  2:921569/1‑150 (MQ=255)
          ccATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTtc                                                                                                                                         <  1:567227/150‑1 (MQ=255)
           cATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTtct                                                                                                                                        <  1:906748/150‑1 (MQ=255)
           cATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTtct                                                                                                                                        >  2:1254180/1‑150 (MQ=255)
            aTTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTtctc                                                                                                                                       >  2:592389/1‑150 (MQ=255)
              tCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCAGTTACAGCTtctgac                                                                                                                                     <  2:434760/150‑4 (MQ=255)
              tCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCAGTTACAGCTtctgac                                                                                                                                     <  1:407225/150‑4 (MQ=255)
               cGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCtcgtc                                                                                                                                    >  2:777208/1‑150 (MQ=255)
                     aCCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGt                                                                                                                              <  2:1106581/150‑1 (MQ=255)
                     aCCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGt                                                                                                                              >  2:1533249/1‑150 (MQ=255)
                      ccAAAGCGTGAAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTa                                                                                                                             >  2:842510/1‑150 (MQ=255)
                       cAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTAc                                                                                                                            <  1:55891/150‑1 (MQ=255)
                        aaaGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTAcc                                                                                                                           <  2:1470500/150‑1 (MQ=255)
                            cGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTc                                                                                                                       <  1:1217367/150‑1 (MQ=255)
                                   caTCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATtcat                                                                                                                <  2:329863/150‑1 (MQ=255)
                                    aTCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATtcatc                                                                                                               <  2:333937/150‑1 (MQ=255)
                                       ttCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAgg                                                                                                            >  1:920106/1‑150 (MQ=255)
                                          ggTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTGATCAGGagc                                                                                                         >  2:1310329/1‑150 (MQ=255)
                                          ggTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGagc                                                                                                         >  1:793166/1‑150 (MQ=255)
                                           gtgtGAATAGTGATTGGTTCACGCTCGGCGATCGCATATTTTTTACGCAGTTCCGCAGAGTTAAGCTGACTGTGTAAACGGTGTGACGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGagcc                                                                                                        >  2:438166/1‑150 (MQ=255)
                                             gtgAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGagccag                                                                                                      <  1:146697/150‑1 (MQ=255)
                                             gtgAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGagccag                                                                                                      >  1:749374/1‑150 (MQ=255)
                                               gAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGagccagcc                                                                                                    >  1:1150542/1‑150 (MQ=255)
                                                aaTAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAgccagcca                                                                                                   <  1:343130/150‑1 (MQ=255)
                                                  tAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAgg                                                                                                 >  2:28421/1‑150 (MQ=255)
                                                   aGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTAATCAGGAGCCAGCCAGGt                                                                                                >  2:885239/1‑150 (MQ=255)
                                                      gATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTgg                                                                                             <  2:1162975/150‑1 (MQ=255)
                                                      gATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTgg                                                                                             >  1:1011000/1‑150 (MQ=255)
                                                       aTTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTggg                                                                                            <  2:793166/150‑1 (MQ=255)
                                                          ggTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCAt                                                                                                                    >  2:999711/1‑123 (MQ=255)
                                                          ggTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCAt                                                                                                                    <  1:999711/123‑1 (MQ=255)
                                                              cACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCg                                                                                     >  1:947457/1‑150 (MQ=255)
                                                                cGATCGGCGATCTCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCggg                                                                                   >  2:625887/1‑150 (MQ=255)
                                                                cGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCggg                                                                                   >  1:49415/1‑150 (MQ=255)
                                                                cGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGCATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCggg                                                                                   >  2:1358064/1‑150 (MQ=255)
                                                                 gATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTTCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGc                                                                                  <  2:1436326/150‑1 (MQ=255)
                                                                 gATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGc                                                                                  <  1:921569/150‑1 (MQ=255)
                                                                 gATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGc                                                                                  <  2:1298119/150‑1 (MQ=255)
                                                                 gATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGGTGGGTGTGCCGGGc                                                                                  <  1:842510/150‑1 (MQ=255)
                                                                    cGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCGGACTGTGTAAACGGGGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGGGTGCCGGGCAAt                                                                               <  1:1225750/150‑1 (MQ=255)
                                                                     ggCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAgccagcca                                                                                                   >  1:147111/1‑129 (MQ=255)
                                                                     ggCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAgccagcca                                                                                                   <  2:147111/129‑1 (MQ=255)
                                                                       cGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCt                                                                            <  2:920106/150‑1 (MQ=255)
                                                                         aTCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTta                                                                          >  2:412501/1‑150 (MQ=255)
                                                                          tCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTtat                                                                         <  1:99061/150‑1 (MQ=255)
                                                                           cGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTtata                                                                        <  1:1358064/150‑1 (MQ=255)
                                                                              atatTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGACGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTTTGCCGGGCAATCATTATAGcc                                                                     >  1:1394022/1‑150 (MQ=255)
                                                                               tatTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCTGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCa                                                                    >  1:429096/1‑150 (MQ=255)
                                                                               tatTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCa                                                                    >  1:1267186/1‑150 (MQ=255)
                                                                                atTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGc                                                                                  >  1:1244871/1‑135 (MQ=255)
                                                                                atTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGc                                                                                  <  2:1244871/135‑1 (MQ=255)
                                                                                        cGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTcc                                                           <  2:884592/150‑1 (MQ=255)
                                                                                                  cATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTAATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTg                                                 <  1:885239/150‑1 (MQ=255)
                                                                                                    tAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGaa                                               >  2:5603/1‑150 (MQ=255)
                                                                                                      gTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATa                                             >  2:672060/1‑150 (MQ=255)
                                                                                                      gTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATa                                             >  2:816130/1‑150 (MQ=255)
                                                                                                       ttAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAg                                            >  1:1504678/1‑150 (MQ=255)
                                                                                                       ttAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAg                                            >  2:1223791/1‑150 (MQ=255)
                                                                                                                   tgtAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTg                                <  1:68356/150‑1 (MQ=255)
                                                                                                                      aaaCGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCg                             >  2:1506040/1‑150 (MQ=255)
                                                                                                                              tgCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGt                     >  1:177793/1‑150 (MQ=255)
                                                                                                                               gCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGCCGTa                    >  2:554787/1‑150 (MQ=255)
                                                                                                                                        ccgacgaGAAGCAGTTACAGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCCGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCa           >  2:321889/7‑150 (MQ=255)
                                                                                                                                         cGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAt          >  2:407264/1‑150 (MQ=255)
                                                                                                                                          ggTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAtt         <  1:816130/150‑1 (MQ=255)
                                                                                                                                          gacgaGAAGCAGTTACAGCTTCTCGTCGGCGGTAGCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGGGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAtt         >  2:516367/5‑150 (MQ=255)
                                                                                                                                           gTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCC‑GGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAtttt       >  1:981536/1‑150 (MQ=255)
                                                                                                                                           gTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCC‑GGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAtttt       >  1:975632/1‑150 (MQ=255)
                                                                                                                                             cAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAttttt      >  2:373865/1‑150 (MQ=255)
                                                                                                                                              aGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCAttttta     >  1:684040/1‑149 (MQ=255)
                                                                                                                                                 aGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCAcc  <  1:1533249/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACC  >  NC_000913/1954848‑1955142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑