Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,670 T→A 8.9% intergenic (‑145/+34) yeeE ← / ← plaP inner membrane protein YeeE/putrescine:H(+) symporter PlaP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6700TA 8.9% 257.5 / 10.6 89intergenic (‑145/+34)yeeE/plaPinner membrane protein YeeE/putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand):  ref base T (39/42);  new base A (4/4);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA  >  NC_000913/2085522‑2085819
                                                                                                                                                    |                                                                                                                                                     
aCATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGtta                                                                                                                                                      >  1:1566044/1‑149 (MQ=255)
 cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCa                                                                                                                                                     <  2:697574/150‑1 (MQ=255)
 cATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCa                                                                                                                                                     <  2:441363/150‑1 (MQ=255)
   tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCTGAGCCAAATGTTCAtt                                                                                                                                                   <  2:604316/150‑1 (MQ=255)
   tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                                   <  2:598717/150‑1 (MQ=255)
    aaaTTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTc                                                                                                                                                  <  2:401669/150‑1 (MQ=255)
     aattaattAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCg                                                                                                                                                 >  2:355135/1‑150 (MQ=255)
     aattaattAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCg                                                                                                                                                 >  2:319559/1‑150 (MQ=255)
         aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATAAAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                             >  1:1491536/1‑150 (MQ=255)
          attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAAGAATAAAAAAACCGGAGCCAAAGGTTAATTCGACTcc                                                                                                                                            >  2:1280503/1‑150 (MQ=255)
            taaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCgg                                                                                                                                          >  2:144612/1‑150 (MQ=255)
            taaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCgg                                                                                                                                          >  1:810615/1‑150 (MQ=255)
                  gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                                    >  1:1095237/1‑150 (MQ=255)
                    tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGGCGAATGAACATTTGGCTCCGGttttttta                                                                                                                                  <  1:1221861/150‑3 (MQ=38)
                    tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                                  >  1:789894/1‑150 (MQ=255)
                      aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAATTTTGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGGCAAATGTTCATTCGACTAAGGTttattattt                                                                                                                                >  1:1036443/1‑149 (MQ=255)
                      aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGCACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAACCCGGAGTCGAATGAACATTTGGCTCCGGtttttttatt                                                                                                                                <  2:1053045/150‑5 (MQ=38)
                      aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatta                                                                                                                                <  1:1464227/150‑1 (MQ=255)
                        tGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATTGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGa                                                                                                                              <  1:598254/150‑1 (MQ=255)
                        tGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGtttttttattct                                                                                                                              <  2:576959/150‑7 (MQ=38)
                          ttAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGaaa                                                                                                                            >  2:1623484/1‑150 (MQ=255)
                            aaTGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAg                                                                                                                          <  1:1205968/150‑1 (MQ=255)
                               gAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatg                                                                                                                       >  2:140590/1‑150 (MQ=255)
                                aGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGATTATTATTAGAAAAgatga                                                                                                                      <  2:887785/150‑1 (MQ=255)
                                 ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                                     >  2:651543/1‑150 (MQ=255)
                                 ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATGCTCAAAATTAGAACAAATGGCTATAAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCTACTCCGGTTTATTATTAGAAAAgatgat                                                                                                                     <  2:548066/150‑1 (MQ=255)
                                 ggAAATTATTCATGACTGGTGGAATGCAGACCAAAAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGTAGCCAAATGTTCATTAGACTCCGGTTTATTATTAGAAAAgatgat                                                                                                                     >  1:1400391/1‑150 (MQ=255)
                                         ttCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAc                                                                                                             >  2:1053714/1‑150 (MQ=255)
                                              gACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCtt                                                                                                        <  2:1574569/150‑1 (MQ=255)
                                              gACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCtt                                                                                                        <  2:1095237/150‑1 (MQ=255)
                                               acgggtgtAATTCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGCGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTc                                                                                                       <  1:1280503/147‑1 (MQ=255)
                                               aCTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTc                                                                                                       >  1:37903/1‑150 (MQ=255)
                                                cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                      >  2:1453416/1‑150 (MQ=255)
                                                cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                      >  1:1114960/1‑150 (MQ=255)
                                                  ggtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGttttttt                                                                                                                                   <  2:75226/119‑2 (MQ=37)
                                                  ggtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGttttttt                                                                                                                                   >  1:75226/1‑118 (MQ=37)
                                                  ggtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTa                                                                                                    >  2:419985/1‑150 (MQ=255)
                                                        aTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCgg                                                                                              <  1:260392/150‑1 (MQ=255)
                                                        aTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCgg                                                                                              <  2:810615/150‑1 (MQ=255)
                                                          gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGaa                                                                                            >  1:832655/1‑150 (MQ=255)
                                                            aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAcc                                                                                          >  1:1132008/1‑150 (MQ=255)
                                                             gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTATTCGTACTGCGGAACCg                                                                                         >  2:468115/1‑150 (MQ=255)
                                                               ccAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGa                                                                                       >  2:90855/1‑150 (MQ=255)
                                                                   tAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAc                                                                                   >  2:638512/1‑150 (MQ=255)
                                                                    aaCCAAATTCTCTAAATTAGAACATATGGTTAGTAATGAGGAGTTCGCTACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTTTTTGATAAGAGGATTATGCGACGGCTTAGTCGTGCGGAACGGGATTACg                                                                                  >  2:453307/1‑150 (MQ=255)
                                                                     aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACgg                                                                                 <  1:303278/150‑1 (MQ=255)
                                                                     aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACgg                                                                                 <  2:421373/150‑1 (MQ=255)
                                                                     aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTAATTCGAATCCGGTTTATTATTAGAAAAGATGATTATGATACGTATTCTTAATGCGTAACCGGATTACgg                                                                                 >  2:732978/1‑150 (MQ=255)
                                                                      ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                                >  1:479167/1‑150 (MQ=255)
                                                                       cAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGaa                                                                               <  1:319559/150‑1 (MQ=255)
                                                                       cAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGaa                                                                               <  2:1105739/150‑1 (MQ=255)
                                                                        aaaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCTGAGCCAAATGTTCATTCTACTCCTTTTTATTATTAGAAAAGATGATTATGCTACTTCTTCGTACTGCGGAACCGGATTACGGAAg                                                                              <  2:267039/150‑1 (MQ=255)
                                                                            tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGctct                                                                          <  1:1465274/150‑1 (MQ=255)
                                                                                tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                                      <  1:544234/150‑1 (MQ=255)
                                                                                        aaCAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAg                                                              >  1:923554/1‑150 (MQ=255)
                                                                                        aaCAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAg                                                              <  2:1378798/150‑1 (MQ=255)
                                                                                          cAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGcc                                                            >  1:1320869/1‑150 (MQ=255)
                                                                                          cAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGcc                                                            <  1:355135/150‑1 (MQ=255)
                                                                                           aaaTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCa                                                           <  1:1240978/150‑1 (MQ=255)
                                                                                            aaTGGTTATTAATGAGGAGTTCGATACAAAAGAATAGAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                      >  2:1253022/1‑106 (MQ=255)
                                                                                            aaTGGTTATTAATGAGGAGTTCGATACAAAAGAATAGAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                      <  1:1253022/106‑1 (MQ=255)
                                                                                            aaTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAg                                                          >  1:195166/1‑150 (MQ=255)
                                                                                             aTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAgg                                                         <  1:871254/150‑1 (MQ=255)
                                                                                             aTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAgg                                                         <  2:789894/150‑1 (MQ=255)
                                                                                             aTGGTTATAAATGAGGATTTCTATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAgg                                                         <  2:1036443/150‑1 (MQ=255)
                                                                                                 ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                                     <  1:559962/150‑1 (MQ=255)
                                                                                                   attaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATc                                                   <  2:37903/150‑1 (MQ=255)
                                                                                                    ttaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                                  <  2:313540/150‑1 (MQ=255)
                                                                                                     taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                                 <  1:1287259/150‑1 (MQ=255)
                                                                                                      aaTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGATGCAAGCCAGGTAAATCAGa                                                >  2:1170748/1‑150 (MQ=255)
                                                                                                      aaTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGa                                                >  2:415338/1‑150 (MQ=255)
                                                                                                         taggacTTCGATACAAAACAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCg                                             <  1:732978/149‑1 (MQ=255)
                                                                                                                 cGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTAGGTGACGCAAGCCAGGTAAATCAGACCGATagcagc                                     >  2:967315/1‑150 (MQ=255)
                                                                                                                      cAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGa                                >  1:1392255/1‑150 (MQ=255)
                                                                                                                        aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGCCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCACGCCAGGTAAATCAGACCGATCGCAGCCCAGCTc                              >  1:634379/1‑150 (MQ=255)
                                                                                                                        aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                              <  1:1053714/150‑1 (MQ=255)
                                                                                                                         aaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCa                             <  1:17349/150‑1 (MQ=255)
                                                                                                                           gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                           >  1:1026209/1‑150 (MQ=255)
                                                                                                                            aaTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                          <  1:90855/150‑1 (MQ=255)
                                                                                                                            aaTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAAGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                          >  2:913365/1‑150 (MQ=255)
                                                                                                                             aataataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                         >  1:1234427/7‑150 (MQ=38)
                                                                                                                                aaaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAg                      <  1:144612/150‑1 (MQ=255)
                                                                                                                                   aaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAc                   <  1:945438/150‑1 (MQ=255)
                                                                                                                                    aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc                  >  1:1385038/1‑150 (MQ=255)
                                                                                                                                    aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc                  <  1:1453416/150‑1 (MQ=255)
                                                                                                                                       cGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAtt               >  1:808979/1‑150 (MQ=255)
                                                                                                                                         gAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGa             <  1:940142/150‑1 (MQ=255)
                                                                                                                                          aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg            <  1:1388585/150‑1 (MQ=255)
                                                                                                                                           gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc           >  1:357033/1‑150 (MQ=255)
                                                                                                                                                aTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTttct      >  1:614140/1‑150 (MQ=255)
                                                                                                                                                    tCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCa  >  1:303375/1‑150 (MQ=255)
                                                                                                                                                    tCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCa  <  2:1114960/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA  >  NC_000913/2085522‑2085819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑