Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,392,500 C→G 5.7% S181T (AGT→ACT)  nuoM ← NADH:quinone oxidoreductase subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,392,5000CG 5.7% 298.0 / 6.6 88S181T (AGT→ACT) nuoMNADH:quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base C (42/41);  new base G (3/2);  total (45/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

ATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGG  >  NC_000913/2392356‑2392649
                                                                                                                                                |                                                                                                                                                     
aTGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGcc                                                                                                                                                  <  2:879503/150‑1 (MQ=255)
 tGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTAATAAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCt                                                                                                                                                 >  2:213375/1‑150 (MQ=255)
    agaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTggg                                                                                                                                              <  2:832622/150‑1 (MQ=255)
    agaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTAGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTggg                                                                                                                                              >  1:1191176/1‑150 (MQ=255)
      aagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgtg                                                                                                                                            <  2:1514388/150‑1 (MQ=255)
       agCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgtgt                                                                                                                                           >  2:1480053/1‑150 (MQ=255)
        gCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTa                                                                                                                                          >  1:1431033/1‑150 (MQ=255)
         cccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCGAGTGGCCTggtgatgt                                                                                                                                         <  2:1517261/150‑7 (MQ=255)
          ccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaaa                                                                                                                                        >  1:843513/1‑150 (MQ=255)
           cAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAAt                                                                                                                                       <  1:62656/150‑1 (MQ=255)
            aGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCATCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAATACCAGCGCCAGGATGTCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATg                                                                                                                                      <  1:1473161/150‑1 (MQ=255)
            aGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATg                                                                                                                                      <  1:831807/150‑1 (MQ=255)
                ttAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaaga                                                                                                                                  <  2:728765/150‑1 (MQ=255)
                ttAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaaga                                                                                                                                  <  2:1140835/150‑1 (MQ=255)
                    cAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt                                                                                                                              >  2:1109097/1‑150 (MQ=255)
                        tATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGtt                                                                                                                          <  2:741553/150‑1 (MQ=255)
                                accacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGt                                                                                                                  <  2:670415/150‑1 (MQ=255)
                                  cacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGACGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa                                                                                                                >  1:528320/1‑150 (MQ=255)
                                    cTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATa                                                                                                              <  1:547770/150‑1 (MQ=255)
                                      ggACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcg                                                                                                            >  2:275391/1‑150 (MQ=255)
                                         cATTGGCGTATTCAGCAGCGCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACGAGGCCACTCGCCTGTGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt                                                                                                         <  2:1212236/150‑1 (MQ=255)
                                                 tATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGt                                                                                                 <  1:568697/150‑1 (MQ=255)
                                                  aTTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTc                                                                                                <  1:952223/150‑1 (MQ=255)
                                                   ttCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCa                                                                                               >  1:860103/1‑150 (MQ=255)
                                                      agcagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                                                                                            >  2:1477623/1‑150 (MQ=255)
                                                          gcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt                                                                                        <  1:440490/150‑1 (MQ=255)
                                                            tctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa                                                                                      <  1:775165/150‑1 (MQ=255)
                                                             ctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                     >  2:229064/1‑150 (MQ=255)
                                                             ctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                     <  1:474152/150‑1 (MQ=255)
                                                               tCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGc                                                                                   >  2:950821/1‑150 (MQ=255)
                                                                 aTAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGccc                                                                                 <  1:146339/150‑1 (MQ=255)
                                                                    gTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCca                                                                              >  1:1099964/1‑150 (MQ=255)
                                                                       gAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg                                                                           >  2:720634/1‑150 (MQ=255)
                                                                       gAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg                                                                           >  2:513285/1‑150 (MQ=255)
                                                                             ccAGACGCCGGTCGCGTTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGAt                                                                     <  2:1358346/150‑1 (MQ=255)
                                                                              cAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATc                                                                    <  1:275391/150‑1 (MQ=255)
                                                                              cAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATc                                                                    <  2:1191176/150‑1 (MQ=255)
                                                                               aGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCa                                                                   <  2:860103/150‑1 (MQ=255)
                                                                                 aCGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGCTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                                                 <  1:563401/150‑1 (MQ=255)
                                                                                  cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                                >  1:1476255/1‑150 (MQ=255)
                                                                                  cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                                <  1:190819/150‑1 (MQ=255)
                                                                                  cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                                >  1:1069311/1‑150 (MQ=255)
                                                                                   gCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                                >  2:417022/1‑149 (MQ=255)
                                                                                   gCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                                <  1:417022/149‑1 (MQ=255)
                                                                                    ccGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                                              <  2:99618/150‑1 (MQ=255)
                                                                                    ccGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                                              >  1:513522/1‑150 (MQ=255)
                                                                                      ggTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTa                                                            <  1:1044839/150‑1 (MQ=255)
                                                                                          gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATc                                                        <  2:762787/150‑1 (MQ=255)
                                                                                           cATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCg                                                       >  1:415863/1‑150 (MQ=255)
                                                                                              tGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCa                                                    <  1:85410/150‑1 (MQ=255)
                                                                                                tAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAcc                                                  >  2:41232/1‑150 (MQ=255)
                                                                                                tAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAcc                                                  <  1:213375/150‑1 (MQ=255)
                                                                                                 aGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                                 <  2:843513/150‑1 (MQ=255)
                                                                                                 aGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                                 >  2:70218/1‑150 (MQ=255)
                                                                                                      aCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatc                                            >  1:1244544/1‑150 (MQ=255)
                                                                                                      aCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatc                                            >  1:1340034/1‑150 (MQ=255)
                                                                                                       cAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatca                                           >  1:1255175/1‑150 (MQ=255)
                                                                                                       cAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatca                                           >  2:1340396/1‑150 (MQ=255)
                                                                                                        aaaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatcat                                          >  1:1190821/1‑150 (MQ=255)
                                                                                                           aaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTc                                       >  1:1064376/1‑150 (MQ=255)
                                                                                                            aCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                      >  1:199952/1‑150 (MQ=255)
                                                                                                               aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCag                                   <  2:1190821/150‑1 (MQ=255)
                                                                                                               aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCag                                   <  1:225616/150‑1 (MQ=255)
                                                                                                                 cgcCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaa                                 <  1:1109097/150‑1 (MQ=255)
                                                                                                                    cAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                      <  2:689993/142‑1 (MQ=255)
                                                                                                                    cAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                      >  1:689993/1‑142 (MQ=255)
                                                                                                                        aTGGCAATCAACATCACCAGGCCACTCGCCTGTGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGTGTTTTTATGCCGCCTGAGTGCTATCAGGTAGTACATCGTGACCAGTATCATTTGCCagaagtagatg                          <  2:1530702/150‑3 (MQ=255)
                                                                                                                         tcgCAATCAACATCACAAGGCAACTCCCCTGGGTATAAATGAATAACTTGCTTGCCGACGTAATACGAGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaagaaga                         >  2:1346481/3‑150 (MQ=255)
                                                                                                                         tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaagaaga                         >  1:782504/1‑150 (MQ=255)
                                                                                                                           gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAAc                       <  1:456171/150‑1 (MQ=255)
                                                                                                                           gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTT‑CCAGAAGAAGAAGAACa                      >  1:902994/1‑150 (MQ=255)
                                                                                                                            cAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                      <  2:513522/150‑1 (MQ=255)
                                                                                                                              aTCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAgg                    >  2:1397905/1‑150 (MQ=255)
                                                                                                                                 aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAAc                 >  2:422860/1‑150 (MQ=255)
                                                                                                                                     cacccAGGCGAGTGGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGt             >  1:282800/4‑150 (MQ=255)
                                                                                                                                      cACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                                 <  2:1229929/113‑1 (MQ=255)
                                                                                                                                      cACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                                 >  1:1229929/1‑113 (MQ=255)
                                                                                                                                      acccAGGCGAGTGGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaag                             >  1:173769/3‑133 (MQ=255)
                                                                                                                                      acccAGGCGAGTGGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaag                             <  2:173769/131‑1 (MQ=255)
                                                                                                                                       cccAGGCGAGTGGCCTGGGTGTAAATGAAGCACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGCGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCg           >  2:392650/2‑150 (MQ=255)
                                                                                                                                        ccAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGa          >  1:778509/1‑150 (MQ=255)
                                                                                                                                        ccAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGa          >  1:1151821/1‑150 (MQ=255)
                                                                                                                                         cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGAt         >  1:151034/1‑150 (MQ=255)
                                                                                                                                          aGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATg        <  1:1480053/150‑1 (MQ=255)
                                                                                                                                             ccACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCa     >  2:92518/1‑150 (MQ=255)
                                                                                                                                             ccACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCa     >  1:597551/1‑150 (MQ=255)
                                                                                                                                              cACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCaa    <  2:1255175/150‑1 (MQ=255)
                                                                                                                                              cACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGCCACCAGCAACATTTCCCAGAAGAAGAAGAACAGGACCATGTAGATGGCaa    >  2:729760/1‑150 (MQ=255)
                                                                                                                                                cTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAgg  <  1:70218/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGG  >  NC_000913/2392356‑2392649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑