Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,413,009 T→A 5.5% K42* (AAG→TAG) ‡ yfbV ← UPF0208 membrane protein YfbV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,413,0090TA 5.5% 348.5 / 7.1 109K42* (AAG→TAG) ‡yfbVUPF0208 membrane protein YfbV
Reads supporting (aligned to +/- strand):  ref base T (49/54);  new base A (2/4);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

Only 100 of 114 total aligned reads displayed.

GCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAG  >  NC_000913/2412860‑2413158
                                                                                                                                                     |                                                                                                                                                     
gCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCtt                                                                                                                                                       <  2:1323686/150‑1 (MQ=255)
   cAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGAt                                                                                                                                                    <  2:320841/150‑1 (MQ=255)
   cAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTG‑CAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATa                                                                                                                                                   <  2:769147/150‑1 (MQ=255)
    aGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATa                                                                                                                                                   <  1:1108108/150‑1 (MQ=255)
    aGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATa                                                                                                                                                   <  1:884632/150‑1 (MQ=255)
     gCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATaa                                                                                                                                                  >  1:465811/1‑150 (MQ=255)
     gCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATaa                                                                                                                                                  >  2:682651/1‑150 (MQ=255)
       caccacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGAGCGCCGGCATAAAACGCAAGCCATAACGCGGCAGATTGATAAcc                                                                                                                                                >  2:1153664/1‑149 (MQ=255)
        accacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAAcgc                                                                                                                                               <  2:180704/150‑1 (MQ=255)
           acaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGTCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgtt                                                                                                                                            <  2:339834/150‑3 (MQ=255)
             aaTCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttat                                                                                                                                          <  1:168145/150‑5 (MQ=255)
              aTCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttatg                                                                                                                                         <  1:646589/150‑6 (MQ=255)
               tCCCTGCATGGGTAAACTTACGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATACCGCGATTTTc                                                                                                                                        <  2:919631/150‑1 (MQ=255)
               tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTc                                                                                                                                        <  1:616406/150‑1 (MQ=255)
                cccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttatgcg                                                                                                                                       <  1:1389002/150‑8 (MQ=255)
                 ccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGa                                                                                                                                      <  1:1117000/150‑1 (MQ=255)
                  cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                                                                                                                     <  1:300172/150‑1 (MQ=255)
                   tGCATGGGTAAACTTAAGGCGAACAGTGCAGTGGCAACTGCCGTGCCAATCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGTCGTCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCTATTTTCGACa                                                                                                                                    <  2:1183280/150‑1 (MQ=255)
                         ggTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGAcc                                                                                                                              <  2:194595/150‑1 (MQ=255)
                          gTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCCTAACGCGTCATCTTGATACAGCGATTTTCGAGAAAGACCg                                                                                                                             >  1:561571/1‑150 (MQ=255)
                          gTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGAt                                                                                                                                                    >  1:1429523/1‑127 (MQ=255)
                          gTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGAt                                                                                                                                                    <  2:1429523/127‑1 (MQ=255)
                                  aaGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAgg                                                                                                                     >  2:1178328/1‑150 (MQ=255)
                                     gCGAACAGGGCAGTGGCAACTGCGGGCCCAAGCGGACCGCCCAGGGCAATCTGGCAGCAGAGAGTAAATACGGCGATGGGCGGCATAAAACGTATCGCATAACTCGTCATCTTTATAACGCGATTTTCGACAAAGACCGGGGCAAGTCGt                                                                                                                  <  2:602088/150‑1 (MQ=255)
                                      cGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATTCGGCGCTCGGCGGCATAAAACGGATCGCATAACGCGTCCTCTTGATAACGCGATTTTCGACACAGACCGGGGCAAGGCggt                                                                                                                 >  1:1109430/1‑148 (MQ=255)
                                      cGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGtt                                                                                                                 <  2:1197126/150‑1 (MQ=255)
                                      cGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGtt                                                                                                                 <  2:1309720/150‑1 (MQ=255)
                                       gAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttt                                                                                                                >  1:198624/1‑150 (MQ=255)
                                         ggccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                              >  2:1165135/5‑150 (MQ=255)
                                          gccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTc                                                                                                             >  1:453516/4‑150 (MQ=255)
                                            gggCAGTGGCGACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCa                                                                                                           >  2:1399714/1‑150 (MQ=255)
                                            gggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCa                                                                                                           >  1:856244/1‑150 (MQ=255)
                                             ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg                                                                                                          <  2:1127299/150‑1 (MQ=255)
                                             ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg                                                                                                          <  1:895517/150‑1 (MQ=255)
                                               cAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCg                                                                                                        <  1:656045/150‑1 (MQ=255)
                                                 gTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                              <  1:480839/142‑1 (MQ=255)
                                                 gTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                              >  2:480839/1‑142 (MQ=255)
                                                  tGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGcc                                                                                                     <  2:1039325/150‑1 (MQ=255)
                                                     cAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACTGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCTGCCACg                                                                                                  <  2:611774/150‑1 (MQ=255)
                                                        cTGCCGGCCCAAGCTGACCGCCCAGGACAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCt                                                                                               >  1:805218/1‑150 (MQ=255)
                                                          gCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCttt                                                                                             >  2:247112/1‑150 (MQ=255)
                                                           ccGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                            <  2:1129660/150‑1 (MQ=255)
                                                             ggCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAg                                                                                          <  2:744531/150‑1 (MQ=255)
                                                              gCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGt                                                                                         <  1:177059/150‑1 (MQ=255)
                                                                 cAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAAt                                                                                      >  2:890180/1‑150 (MQ=255)
                                                                   aGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGc                                                                                    >  1:1485654/1‑150 (MQ=255)
                                                                            cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACATAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                           <  1:1209510/150‑1 (MQ=255)
                                                                            cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                           <  1:512612/150‑1 (MQ=255)
                                                                            cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                           <  2:1494784/150‑1 (MQ=255)
                                                                            cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                           <  1:1306253/150‑1 (MQ=255)
                                                                              cAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCg                                                                         <  1:1178328/150‑1 (MQ=255)
                                                                                  gCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAAc                                                                     >  2:36595/1‑150 (MQ=255)
                                                                                   cAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCGTAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACa                                                                    >  2:232518/1‑150 (MQ=255)
                                                                                     aTCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAg                                                                  >  1:1470132/1‑150 (MQ=255)
                                                                                     aTCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAg                                                                  <  2:1470132/150‑1 (MQ=255)
                                                                                        tGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTa                                                               >  2:291435/1‑150 (MQ=255)
                                                                                            agcagAGAGTAAATACGGCGATCGGCGGCATAAAAAGGATCGCATAACGCGTCATATTGATAAAGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaa                                                           >  2:292044/1‑150 (MQ=255)
                                                                                             gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                          >  2:431204/1‑150 (MQ=255)
                                                                                             gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                          >  2:171387/1‑150 (MQ=255)
                                                                                             gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                          >  2:643878/1‑150 (MQ=255)
                                                                                             gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                          >  2:845933/1‑150 (MQ=255)
                                                                                                    gTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACa                                                   >  2:464410/1‑150 (MQ=255)
                                                                                                      aaaTACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGa                                                 >  1:1039185/1‑150 (MQ=255)
                                                                                                       aaTACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGaa                                                >  1:1192526/1‑150 (MQ=255)
                                                                                                           cGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGCCCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGAt                                            >  1:1305725/1‑150 (MQ=255)
                                                                                                           cGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAACTTAACAGAACGAt                                            <  2:1276804/150‑1 (MQ=255)
                                                                                                              cGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTAt                                         >  1:1141189/1‑150 (MQ=255)
                                                                                                                   ggcggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGc                                    >  1:1576438/1‑150 (MQ=255)
                                                                                                                    gcggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCg                                   <  2:840416/150‑1 (MQ=255)
                                                                                                                      ggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGtt                                 <  1:1153664/150‑1 (MQ=255)
                                                                                                                        cATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGa                               <  2:856244/150‑1 (MQ=255)
                                                                                                                           aaaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACAt                            >  1:1104077/1‑150 (MQ=255)
                                                                                                                           aaaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACAt                            >  2:774624/1‑150 (MQ=255)
                                                                                                                           aaaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTATTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACAt                            >  1:1284923/1‑150 (MQ=255)
                                                                                                                            aaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATg                           <  2:705394/150‑1 (MQ=255)
                                                                                                                              aCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGGCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCt                         <  2:237133/150‑1 (MQ=255)
                                                                                                                                ggATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTc                       <  1:682651/150‑1 (MQ=255)
                                                                                                                                 gATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATcc                                       <  2:1146032/133‑1 (MQ=255)
                                                                                                                                 gATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATcc                                       >  1:1146032/1‑133 (MQ=255)
                                                                                                                                  aTCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCAc                     >  1:864245/1‑150 (MQ=255)
                                                                                                                                   tCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCAcc                    <  1:859152/150‑1 (MQ=255)
                                                                                                                                   tCGCATAACGCGTCATCTTGATAACGCGATTTTAGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCGAAGTCTTTGAGTAATGCTGTACCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCAcc                    <  2:716570/150‑1 (MQ=255)
                                                                                                                                     gaaaatcgcgTTATCAAGATGACGCGATTTTCGACAAAGACCGGGGCAAGGCTTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAAAAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTGGACATTCTTCACCTc                  >  2:589632/7‑150 (MQ=255)
                                                                                                                                     gaaaatcgcgTTATCAAGATGACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  2:1395297/7‑150 (MQ=255)
                                                                                                                                     gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  2:1111910/1‑150 (MQ=255)
                                                                                                                                     gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  2:1365780/1‑150 (MQ=255)
                                                                                                                                     gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  2:478209/1‑150 (MQ=255)
                                                                                                                                      cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                 <  1:1399714/150‑1 (MQ=255)
                                                                                                                                      cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                 <  2:172202/150‑1 (MQ=255)
                                                                                                                                      cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                 <  2:1039185/150‑1 (MQ=255)
                                                                                                                                        tAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAAc               >  2:903859/1‑150 (MQ=255)
                                                                                                                                        tAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAAc               <  1:890180/150‑1 (MQ=255)
                                                                                                                                               tCATCTTGATAACGCGATTTTCGACAAAGACCGGTGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACat        <  2:1192526/150‑1 (MQ=255)
                                                                                                                                                  tCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACAtata     <  1:431204/150‑1 (MQ=255)
                                                                                                                                                  tCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACAtata     <  1:643878/150‑1 (MQ=255)
                                                                                                                                                  tCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACAtata     >  2:1477433/1‑150 (MQ=255)
                                                                                                                                                   cTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATaa    >  1:1220381/1‑150 (MQ=255)
                                                                                                                                                    ttGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATaaa   >  2:917214/1‑150 (MQ=255)
                                                                                                                                                     tGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAg  <  2:356669/150‑1 (MQ=255)
                                                                                                                                                     tGATAACGCGAATTTCGACAAAGACCGGGGCAAGGCGTGTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCCCCTCCAGTGCACAGATAAAg  >  2:487523/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAG  >  NC_000913/2412860‑2413158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑