Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,413,013 A→G 5.6% V40V (GTT→GTC)  yfbV ← UPF0208 membrane protein YfbV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,413,0130AG 5.6% 330.7 / 4.6 106V40V (GTT→GTC) yfbVUPF0208 membrane protein YfbV
Reads supporting (aligned to +/- strand):  ref base A (50/50);  new base G (2/4);  total (52/54)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTC  >  NC_000913/2412863‑2413162
                                                                                                                                                      |                                                                                                                                                     
cAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTG‑CAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATa                                                                                                                                                       <  2:769147/150‑1 (MQ=255)
 aGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATa                                                                                                                                                       <  1:1108108/150‑1 (MQ=255)
  gCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATaa                                                                                                                                                      >  2:682651/1‑150 (MQ=255)
  gCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATaa                                                                                                                                                      >  1:465811/1‑150 (MQ=255)
    caccacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGAGCGCCGGCATAAAACGCAAGCCATAACGCGGCAGATTGATAAcc                                                                                                                                                    >  2:1153664/1‑149 (MQ=255)
     accacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAAcgc                                                                                                                                                   <  2:180704/150‑1 (MQ=255)
        acaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGTCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgtt                                                                                                                                                <  2:339834/150‑3 (MQ=255)
          aaTCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttat                                                                                                                                              <  1:168145/150‑5 (MQ=255)
           aTCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttatg                                                                                                                                             <  1:646589/150‑6 (MQ=255)
            tCCCTGCATGGGTAAACTTACGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATACCGCGATTTTc                                                                                                                                            <  2:919631/150‑1 (MQ=255)
            tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTc                                                                                                                                            <  1:616406/150‑1 (MQ=255)
              ccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGa                                                                                                                                          <  1:1117000/150‑1 (MQ=255)
               cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                                                                                                                         <  1:300172/150‑1 (MQ=255)
                tGCATGGGTAAACTTAAGGCGAACAGTGCAGTGGCAACTGCCGTGCCAATCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGTCGTCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCTATTTTCGACa                                                                                                                                        <  2:1183280/150‑1 (MQ=255)
                       gTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCCTAACGCGTCATCTTGATACAGCGATTTTCGAGAAAGACCg                                                                                                                                 >  1:561571/1‑150 (MQ=255)
                            cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCa                                                                                                                            >  1:1328962/1‑150 (MQ=255)
                               aaGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAgg                                                                                                                         >  2:1178328/1‑150 (MQ=255)
                                  gCGAACAGGGCAGTGGCAACTGCGGGCCCAAGCGGACCGCCCAGGGCAATCTGGCAGCAGAGAGTAAATACGGCGATGGGCGGCATAAAACGTATCGCATAACTCGTCATCTTTATAACGCGATTTTCGACAAAGACCGGGGCAAGTCGt                                                                                                                      <  2:602088/150‑1 (MQ=255)
                                   cGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATTCGGCGCTCGGCGGCATAAAACGGATCGCATAACGCGTCCTCTTGATAACGCGATTTTCGACACAGACCGGGGCAAGGCggt                                                                                                                     >  1:1109430/1‑148 (MQ=255)
                                   cGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGtt                                                                                                                     <  2:1197126/150‑1 (MQ=255)
                                   cGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGtt                                                                                                                     <  2:1309720/150‑1 (MQ=255)
                                      ggccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                                  >  2:1165135/5‑150 (MQ=255)
                                       gccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTc                                                                                                                 >  1:453516/4‑150 (MQ=255)
                                         gggCAGTGGCGACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCa                                                                                                               >  2:1399714/1‑150 (MQ=255)
                                         gggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCa                                                                                                               >  1:856244/1‑150 (MQ=255)
                                         gggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCACCAGAGAGTAAATACGCCGATCGGCGGCATAAAACGGATCGCATAACTCGTCATCATCATAACGCGATTTTCGACAAAGAACGGCGCAAGGCGTTTTTACa                                                                                                               >  1:716570/1‑150 (MQ=255)
                                          ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg                                                                                                              <  2:1127299/150‑1 (MQ=255)
                                          ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg                                                                                                              <  1:895517/150‑1 (MQ=255)
                                            cAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCg                                                                                                            <  1:656045/150‑1 (MQ=255)
                                              gTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                                  <  1:480839/142‑1 (MQ=255)
                                              gTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                                  >  2:480839/1‑142 (MQ=255)
                                               tGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGcc                                                                                                         <  2:1328962/150‑1 (MQ=255)
                                               tGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGcc                                                                                                         <  2:1039325/150‑1 (MQ=255)
                                                  cAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACTGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCTGCCACg                                                                                                      <  2:611774/150‑1 (MQ=255)
                                                     cTGCCGGCCCAAGCTGACCGCCCAGGACAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCt                                                                                                   >  1:805218/1‑150 (MQ=255)
                                                       gCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCttt                                                                                                 >  2:247112/1‑150 (MQ=255)
                                                        ccGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                                <  2:1129660/150‑1 (MQ=255)
                                                          ggCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAg                                                                                              <  2:744531/150‑1 (MQ=255)
                                                           gCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGt                                                                                             <  1:177059/150‑1 (MQ=255)
                                                              cAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAAt                                                                                          >  2:890180/1‑150 (MQ=255)
                                                                aGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGc                                                                                        >  1:1485654/1‑150 (MQ=255)
                                                                        gCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCg                                                                                <  1:497295/150‑1 (MQ=255)
                                                                         cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACATAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                               <  1:1209510/150‑1 (MQ=255)
                                                                         cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                               <  1:1306253/150‑1 (MQ=255)
                                                                         cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                               <  1:512612/150‑1 (MQ=255)
                                                                         cccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCgg                                                                               <  2:1494784/150‑1 (MQ=255)
                                                                           cAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCg                                                                             <  1:1178328/150‑1 (MQ=255)
                                                                                cAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCGTAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACa                                                                        >  2:232518/1‑150 (MQ=255)
                                                                                  aTCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAg                                                                      >  1:1470132/1‑150 (MQ=255)
                                                                                  aTCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAg                                                                      <  2:1470132/150‑1 (MQ=255)
                                                                                     tGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTa                                                                   >  2:291435/1‑150 (MQ=255)
                                                                                         agcagAGAGTAAATACGGCGATCGGCGGCATAAAAAGGATCGCATAACGCGTCATATTGATAAAGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaa                                                               >  2:292044/1‑150 (MQ=255)
                                                                                          gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                              >  2:845933/1‑150 (MQ=255)
                                                                                          gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                              >  2:643878/1‑150 (MQ=255)
                                                                                          gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                              >  2:431204/1‑150 (MQ=255)
                                                                                          gcagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaa                                                              >  2:171387/1‑150 (MQ=255)
                                                                                             gagagTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAAtt                                                           >  1:898987/1‑150 (MQ=255)
                                                                                                 gTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACa                                                       >  2:464410/1‑150 (MQ=255)
                                                                                                   aaaTACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGa                                                     >  1:1039185/1‑150 (MQ=255)
                                                                                                    aaTACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGaa                                                    >  1:1192526/1‑150 (MQ=255)
                                                                                                        cGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGCCCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGAt                                                >  1:1305725/1‑150 (MQ=255)
                                                                                                        cGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAACTTAACAGAACGAt                                                <  2:1276804/150‑1 (MQ=255)
                                                                                                           cGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTAt                                             >  1:1141189/1‑150 (MQ=255)
                                                                                                                ggcggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGc                                        >  1:1576438/1‑150 (MQ=255)
                                                                                                                 gcggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCg                                       >  1:172202/1‑150 (MQ=255)
                                                                                                                 gcggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCg                                       <  2:840416/150‑1 (MQ=255)
                                                                                                                   ggcATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGtt                                     <  1:1153664/150‑1 (MQ=255)
                                                                                                                     cATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGa                                   <  2:856244/150‑1 (MQ=255)
                                                                                                                        aaaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACAt                                >  2:774624/1‑150 (MQ=255)
                                                                                                                        aaaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACAt                                >  1:1104077/1‑150 (MQ=255)
                                                                                                                        aaaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTATTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACAt                                >  1:1284923/1‑150 (MQ=255)
                                                                                                                         aaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATg                               <  2:705394/150‑1 (MQ=255)
                                                                                                                           aCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGGCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCt                             <  2:237133/150‑1 (MQ=255)
                                                                                                                             ggATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTc                           <  1:682651/150‑1 (MQ=255)
                                                                                                                              gATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATcc                                           >  1:1146032/1‑133 (MQ=255)
                                                                                                                               aTCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCAc                         >  1:864245/1‑150 (MQ=255)
                                                                                                                                tCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCAcc                        <  1:859152/150‑1 (MQ=255)
                                                                                                                                tCGCATAACGCGTCATCTTGATAACGCGATTTTAGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCGAAGTCTTTGAGTAATGCTGTACCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCAcc                        <  2:716570/150‑1 (MQ=255)
                                                                                                                                 cGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCt                       <  2:278885/150‑1 (MQ=255)
                                                                                                                                  gaaaatcgcgTTATCAAGATGACGCGATTTTCGACAAAGACCGGGGCAAGGCTTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAAAAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTGGACATTCTTCACCTc                      >  2:589632/7‑150 (MQ=255)
                                                                                                                                  gaaaatcgcgTTATCAAGATGACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                      >  2:1395297/7‑150 (MQ=255)
                                                                                                                                  gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                      >  2:478209/1‑150 (MQ=255)
                                                                                                                                  gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                      >  2:1111910/1‑150 (MQ=255)
                                                                                                                                   cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                     <  2:1039185/150‑1 (MQ=255)
                                                                                                                                   cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                     <  2:172202/150‑1 (MQ=255)
                                                                                                                                   cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                     <  1:1399714/150‑1 (MQ=255)
                                                                                                                                     tAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAAc                   >  2:903859/1‑150 (MQ=255)
                                                                                                                                     tAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAAc                   <  1:890180/150‑1 (MQ=255)
                                                                                                                                          cgTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTcac              <  2:453516/150‑1 (MQ=255)
                                                                                                                                            tCATCTTGATAACGCGATTTTCGACAAAGACCGGTGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACat            <  2:1192526/150‑1 (MQ=255)
                                                                                                                                               tCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACAtata         >  2:1477433/1‑150 (MQ=255)
                                                                                                                                               tCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACAtata         <  1:431204/150‑1 (MQ=255)
                                                                                                                                                cTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATaa        >  1:1220381/1‑150 (MQ=255)
                                                                                                                                                 ttGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATaaa       >  2:917214/1‑150 (MQ=255)
                                                                                                                                                  tGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAg      <  2:356669/150‑1 (MQ=255)
                                                                                                                                                  tGATAACGCGAATTTCGACAAAGACCGGGGCAAGGCGTGTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCCCCTCCAGTGCACAGATAAAg      >  2:487523/1‑150 (MQ=255)
                                                                                                                                                   gATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGa     >  1:1056851/1‑150 (MQ=255)
                                                                                                                                                   gATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGa     >  2:658165/1‑150 (MQ=255)
                                                                                                                                                     tAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGAtt   <  1:171387/150‑1 (MQ=255)
                                                                                                                                                      aaCGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTc  >  2:855588/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTC  >  NC_000913/2412863‑2413162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑