BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,434,097	T→C	12.9%	A215A (GCA→GCG)	dedA ←	DedA family protein DedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,097	0	T	C	12.9%	252.5 / 26.3	101	A215A (GCA→GCG)	dedA	DedA family protein DedA
Reads supporting (aligned to +/- strand): ref base T (46/42); new base C (6/7); total (52/49)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-02

Only 100 of 102 total aligned reads displayed.

CTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACA  >  NC_000913/2433948‑2434245

cTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGt                                                                                                                                                      >  1:181603/1‑150 (MQ=255)

     tCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGc                                                                                                                                                 >  1:1233156/1‑150 (MQ=255)

      cGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCa                                                                                                                                                >  1:1465893/1‑150 (MQ=255)

      cGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCa                                                                                                                                                >  2:343635/1‑150 (MQ=255)

       gATGCCAGTTTTATCTTTGGGGACACATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAg                                                                                                                                               >  1:1574579/1‑150 (MQ=255)

         tGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                                                                             <  1:1029785/150‑4 (MQ=255)

         tGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                                                                             <  1:374020/150‑4 (MQ=255)

          gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATACCAGCCCGAAAGTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaat                                                                                                                                            <  2:456957/150‑5 (MQ=255)

          gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCCCGCGCCGCaaaat                                                                                                                                            <  1:1531491/150‑5 (MQ=255)

          gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaat                                                                                                                                            <  1:497143/150‑5 (MQ=255)

          gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaat                                                                                                                                            <  1:996278/150‑5 (MQ=255)

           ccAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGcgcg                                                                                                                                           >  2:1116934/1‑150 (MQ=255)

              gTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGttt                                                                                                                                        <  2:913594/150‑1 (MQ=255)

               ttttATCTTTTGGTAAGCATAATGCCATTTTTGCCCCCAAAAGACCATGAATGTTGCACATTAAAACATAACATCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCTCGTGCCGCATCGCGTTtg                                                                                                                                       <  1:1536659/150‑1 (MQ=255)

               ttttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtg                                                                                                                                       <  2:982654/150‑1 (MQ=255)

               ttttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtg                                                                                                                                       <  1:1542419/150‑1 (MQ=255)

                 ttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtgtg                                                                                                                                     <  1:569932/150‑1 (MQ=255)

                   aTCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGAc                                                                                                                                   >  1:108329/1‑150 (MQ=255)

                    tCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACg                                                                                                                                  >  1:206928/1‑150 (MQ=255)

                     cTTTGGGGACGCATAATGCCATTTTTGGCCCGAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAATAGTGGTCGTACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACgg                                                                                                                                 <  2:156047/150‑1 (MQ=255)

                     cTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACgg                                                                                                                                 >  2:856419/1‑150 (MQ=255)

                     cTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACgg                                                                                                                                 >  1:1271573/1‑150 (MQ=255)

                         ggggACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGatta                                                                                                                             >  2:1523669/1‑150 (MQ=255)

                            gACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGGCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTAttt                                                                                                                          <  1:1350848/150‑1 (MQ=255)

                                cATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTTGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGAt                                                                                                                      >  1:1477535/1‑150 (MQ=255)

                                     tGCCATTTTTGCCCCCACCAGACCATGCATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAACAACGTGGTCGCACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGGGACGGAGTATTTCGATGCCGc                                                                                                                 >  2:671332/1‑150 (MQ=255)

                                     tGCCATTTTTGCCCCCAACCGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACGTTGGATAAAAAAGGGGTCGACCCGCGGAGGTACTTTTTATTTTGCGGCGCGTGCCGCCGCGCGGTTGTGCCGGATTATTTCGATGCCGc                                                                                                                 >  2:666025/1‑150 (MQ=255)

                                      gCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAATTGGTCGAACCCCGAATTTAATTTTAATTTTGCGCCGCGTCCCCCACCCCTTTTGTGCCGTATTATTACGATGACGcc                                                                                                                >  2:310326/1‑150 (MQ=255)

                                         aTTTTTGCCCCCAACAGACCATGAATGTTGCACATTTACAGATAACAGCCCGAAACTTTGTATAAAAAAGTGGTCGAACCGCGGAGTTTCTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCgg                                                                                                             <  1:1098741/150‑1 (MQ=255)

                                         aTTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCgg                                                                                                             <  1:320439/150‑1 (MQ=255)

                                          tttttGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGc                                                                                                            <  2:489342/150‑1 (MQ=255)

                                              tGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaaaa                                                                                                        <  1:1195907/150‑1 (MQ=255)

                                               gCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAAt                                                                                                       <  1:780075/150‑1 (MQ=255)

                                                cccccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATa                                                                                                      >  1:168917/1‑150 (MQ=255)

                                                  cccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGa                                                                                                    >  2:562533/1‑150 (MQ=255)

                                                      aCAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAAcc                                                                                                <  1:1523669/150‑1 (MQ=255)

                                                         gACCATGAATGTTGCACATTAAAACATAACAGCCCGAAGCTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACcaca                                                                                             >  1:992246/1‑150 (MQ=255)

                                                                aaTGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATcc                                                                                      >  2:815331/1‑150 (MQ=255)

                                                                 aTGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATccc                                                                                     <  1:1129275/150‑1 (MQ=255)

                                                                   gTTGCACATTAAAACATAACAGACAGAAACTTTGGATAAAAAAGTGGTCGAACAGAGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcga                                                                                   >  2:485123/1‑150 (MQ=255)

                                                                    ttGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgac                                                                                  <  1:343635/150‑1 (MQ=255)

                                                                       cacaTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGAt                                                                               >  1:239970/1‑150 (MQ=255)

                                                                        acaTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATc                                                                              <  2:1271573/150‑1 (MQ=255)

                                                                            tAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcagca                                                                          <  2:1521842/150‑1 (MQ=255)

                                                                             aaaaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcagcag                                                                         >  1:149809/1‑150 (MQ=255)

                                                                                 cATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTa                                                                     >  2:1353847/1‑150 (MQ=255)

                                                                                      cAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGtt                                                                >  1:1471063/1‑150 (MQ=255)

                                                                                        gCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTAt                                                              >  1:1167104/1‑150 (MQ=255)

                                                                                         cccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGtatta                                                             >  1:1604629/1‑146 (MQ=255)

                                                                                          ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc                                                            >  1:1499225/1‑150 (MQ=255)

                                                                                            gAAACTTTTGATAAAAAAGTGGTCTAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCTTACGATCAGTAGCTTAAGGTTATCCTg                                                          <  2:693876/150‑1 (MQ=255)

                                                                                            gAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTg                                                          >  1:1247906/1‑150 (MQ=255)

                                                                                               aCTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAAc                                                       <  1:598244/150‑1 (MQ=255)

                                                                                                cTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAAcc                                                      >  1:150759/1‑150 (MQ=255)

                                                                                                cTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAAcc                                                      >  2:217820/1‑150 (MQ=255)

                                                                                                   tGGCTAAAAAAGGGGGCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCTTTTGTTACGGATTATTTCGATGACGCCCGGCAAAATAGAAAACAGAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATc                                                   <  2:1465893/150‑1 (MQ=255)

                                                                                                         aaaaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGAtt                                             >  2:885351/1‑150 (MQ=255)

                                                                                                         aaaaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCAAGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGAtt                                             >  2:417856/1‑150 (MQ=255)

                                                                                                           aaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGt                                           <  2:1233156/150‑1 (MQ=255)

                                                                                                            aaGTGGTCGAACCGCGGAGTTACTTTTAATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGCGATTGTa                                          >  2:1138621/1‑150 (MQ=255)

                                                                                                              gTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTAcc                                        <  2:1076315/150‑1 (MQ=255)

                                                                                                              gTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTAcc                                        >  1:1599469/1‑150 (MQ=255)

                                                                                                                ggTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCTCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGCTCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCga                                      >  2:1530522/1‑150 (MQ=255)

                                                                                                                ggTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCga                                      >  2:19152/1‑150 (MQ=255)

                                                                                                                 gTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaa                                     <  1:519702/150‑1 (MQ=255)

                                                                                                                 gTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaa                                     >  1:313656/1‑150 (MQ=255)

                                                                                                                  tCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaag                                    <  1:598959/150‑1 (MQ=255)

                                                                                                                  tCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaag                                    <  2:1599469/150‑1 (MQ=255)

                                                                                                                  tCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaag                                    <  2:181603/150‑1 (MQ=255)

                                                                                                                      aCCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAAt                                <  1:1284443/150‑1 (MQ=255)

                                                                                                                       ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATa                               >  2:1565632/1‑150 (MQ=255)

                                                                                                                         gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATTGGGATTGTACCGAAGAAATAGc                             >  2:1120096/1‑150 (MQ=255)

                                                                                                                         gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGc                             >  2:1384818/1‑150 (MQ=255)

                                                                                                                         gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGc                             <  1:249232/150‑1 (MQ=255)

                                                                                                                         gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGc                             >  2:1531719/1‑150 (MQ=255)

                                                                                                                            gAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTg                          <  2:1494157/150‑1 (MQ=255)

                                                                                                                                tACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAgg                                                                  >  2:25604/1‑106 (MQ=255)

                                                                                                                                tACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAgg                                                                  <  1:25604/106‑1 (MQ=255)

                                                                                                                                tACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTa                      <  1:1557835/150‑1 (MQ=255)

                                                                                                                                tACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTa                      <  1:1353847/150‑1 (MQ=255)

                                                                                                                                tACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTa                      >  1:1345756/1‑150 (MQ=255)

                                                                                                                                   tttttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGt                   <  1:310326/150‑1 (MQ=255)

                                                                                                                                    ttttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTa                  <  1:1116934/150‑1 (MQ=255)

                                                                                                                                    ttttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTa                  <  1:815331/150‑1 (MQ=255)

                                                                                                                                       tATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaa               >  1:24997/1‑150 (MQ=255)

                                                                                                                                       cgcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaa               >  2:194605/6‑150 (MQ=255)

                                                                                                                                        gcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa              >  1:642625/5‑150 (MQ=255)

                                                                                                                                        aTTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa              >  1:207055/1‑150 (MQ=255)

                                                                                                                                        aTTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa              >  2:608157/1‑150 (MQ=255)

                                                                                                                                        aTTTTGCGGCCCGTGCCGAACCCCTTTTGTGACGGATTATTTAGATGACGCCCGGCAAAATAGAAACCACAATAATCCAGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa              >  2:540693/1‑150 (MQ=255)

                                                                                                                                         ttttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAg             <  2:25155/150‑1 (MQ=255)

                                                                                                                                         cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaacg             >  2:1474805/4‑148 (MQ=255)

                                                                                                                                         cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAg             >  2:207287/4‑150 (MQ=255)

                                                                                                                                         cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAg             >  2:1074463/4‑150 (MQ=255)

                                                                                                                                         cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGACTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAg             >  1:1467346/4‑150 (MQ=255)

                                                                                                                                            tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCTATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAg          <  1:666025/150‑1 (MQ=255)

                                                                                                                                            tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTCAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAg          <  1:671332/150‑1 (MQ=255)

                                                                                                                                            tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAg          <  1:19152/150‑1 (MQ=255)

                                                                                                                                                gcgcGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTAcc      <  2:108329/150‑1 (MQ=255)

                                                                                                                                                    gTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCcaca  <  1:1608196/150‑1 (MQ=255)

CTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACA  >  NC_000913/2433948‑2434245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`