Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,491,266 C→G 7.8% E229Q (GAA→CAA) ‡ oxc ← oxalyl‑CoA decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,491,2660CG 7.8% 288.8 / 12.8 90E229Q (GAA→CAA) ‡oxcoxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (46/37);  new base G (5/2);  total (51/39)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCTT  >  NC_000913/2491118‑2491415
                                                                                                                                                    |                                                                                                                                                      
ccAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                                       >  1:1280218/1‑150 (MQ=255)
  aacaaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatc                                                                                                                                                     >  1:1056065/1‑150 (MQ=255)
          tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                                            <  2:409581/150‑1 (MQ=255)
          tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                                            <  1:55634/150‑1 (MQ=255)
             cAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAAt                                                                                                                                         >  2:978990/1‑150 (MQ=255)
               aCGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAc                                                                                                                                       <  1:1270753/150‑1 (MQ=255)
                  cgttcgtttGCCCTGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgcc                                                                                                                                    >  1:1001951/7‑150 (MQ=255)
                  cgttcgtttGCCCTGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgcc                                                                                                                                    >  1:31026/7‑150 (MQ=255)
                   gttcgtttGCCCTGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgccg                                                                                                                                   >  2:1532077/6‑150 (MQ=255)
                   cAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgccg                                                                                                                                   >  2:1314087/1‑150 (MQ=255)
                   cAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCAC‑AAGCTGATGAACAGCTTCGTGAAtttattg                                                                                                                                   <  2:353682/150‑7 (MQ=38)
                   cAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCAC‑AAGCTGATGAACAGCTTCGTGAAtttattg                                                                                                                                   <  2:419229/150‑7 (MQ=38)
                     gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCcgcg                                                                                                                                 <  1:82055/150‑1 (MQ=255)
                     gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCcgcg                                                                                                                                 >  2:1237606/1‑150 (MQ=255)
                          tGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCttt                                                                                                                            >  1:593809/1‑150 (MQ=255)
                           gCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTg                                                                                                                           <  2:1263709/150‑1 (MQ=255)
                           gCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTg                                                                                                                           <  2:373270/150‑1 (MQ=255)
                            ccAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGc                                                                                                                          >  1:1411995/1‑150 (MQ=255)
                             cAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGcc                                                                                                                         <  2:300601/150‑1 (MQ=255)
                               gggCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCTATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCaa                                                                                                                       >  2:181861/1‑150 (MQ=255)
                                 gCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAgg                                                                                                                     <  2:1179256/150‑1 (MQ=255)
                                   aaaCGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAg                                                                                                                      <  1:634561/147‑1 (MQ=255)
                                   aaaCGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAg                                                                                                                      >  2:634561/1‑147 (MQ=255)
                                    cacgaacgCTCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGCCATTGGAAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGAGCCTTTGCCAAGGAta                                                                                                                  >  2:649690/2‑150 (MQ=255)
                                    aacgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAta                                                                                                                  >  2:1393420/1‑150 (MQ=255)
                                      cgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaat                                                                                                                <  2:303958/150‑1 (MQ=255)
                                      cgaacgCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaat                                                                                                                >  2:1128609/1‑150 (MQ=255)
                                           gcgcAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATg                                                                                                           <  2:1311147/150‑1 (MQ=255)
                                            cgcAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATgg                                                                                                          >  2:1450900/1‑150 (MQ=255)
                                              cAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGcc                                                                                                        >  2:200338/1‑150 (MQ=255)
                                                 cTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGtt                                                                                                     >  1:1608699/1‑150 (MQ=255)
                                                    ccGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAg                                                                                                  >  2:512213/1‑150 (MQ=255)
                                                    ccGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAg                                                                                                  <  2:566492/150‑1 (MQ=255)
                                                       cAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCtt                                                                                               >  1:131197/1‑150 (MQ=255)
                                                            aGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCt                                                                                          <  2:1056065/150‑1 (MQ=255)
                                                             gTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGc                                                                                                 >  1:692941/1‑142 (MQ=255)
                                                             gTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGc                                                                                                 <  2:692941/142‑1 (MQ=255)
                                                             gTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTa                                                                                         <  1:489381/150‑1 (MQ=255)
                                                              tGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACATTCAATAAATTCACGAAGCTGTTCATCAGCGT‑GGGAATACGCCGCGCCTTTGCCAAGGCTAATTAAGGGCCGTGCAGCTTTTGCTaa                                                                                        >  2:329366/1‑150 (MQ=255)
                                                                gATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaaa                                                                                      >  1:421541/1‑150 (MQ=255)
                                                                 aTGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAg                                                                                     <  2:1280218/150‑1 (MQ=255)
                                                                   gCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCg                                                                                   >  2:52193/1‑150 (MQ=255)
                                                                    cGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGa                                                                                  <  1:1237606/150‑1 (MQ=255)
                                                                    cGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGa                                                                                  >  1:332261/1‑150 (MQ=255)
                                                                        tCTTCAAGGATCCCTTTCGCCATAGACATTGGAAGGAATGGAATCTGAGCACTTTCAATAAATTCAAGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAAtt                                                                              >  1:1467513/1‑150 (MQ=255)
                                                                         cTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCATTTGCCAAGGATAATTAATGGCCGTTAAGCTTTTGCTAAAAGCGAAATTg                                                                             >  1:24251/1‑150 (MQ=255)
                                                                          ttCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgc                                                                            >  1:1331417/1‑150 (MQ=255)
                                                                          ttCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgc                                                                            >  2:1116276/1‑150 (MQ=255)
                                                                           tCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTgcg                                                                           <  1:1532077/150‑1 (MQ=255)
                                                                                  tCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtga                                                                    >  2:1379708/1‑150 (MQ=255)
                                                                                  tCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtga                                                                    >  2:530236/1‑150 (MQ=255)
                                                                                     cTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactg                                                                 >  2:1532073/1‑150 (MQ=255)
                                                                                       ttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactgac                                                               >  1:107395/1‑150 (MQ=255)
                                                                                             aTAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCggg                                                         >  2:1115915/1‑150 (MQ=255)
                                                                                             aTAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCggg                                                         >  2:1609113/1‑150 (MQ=255)
                                                                                              tAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGc                                                        <  2:1428582/150‑1 (MQ=255)
                                                                                              tAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGc                                                        >  1:498072/1‑150 (MQ=255)
                                                                                               aGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCa                                                       <  2:107395/150‑1 (MQ=255)
                                                                                                gACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGc                                                                                    <  2:853441/120‑1 (MQ=255)
                                                                                                gACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGc                                                                                    >  1:853441/1‑120 (MQ=255)
                                                                                                gACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCAt                                                      >  1:1415332/1‑150 (MQ=255)
                                                                                                 aCATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATg                                                     <  2:972853/150‑1 (MQ=255)
                                                                                                   aTTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGc                                                   <  1:1128609/150‑1 (MQ=255)
                                                                                                   aTTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGc                                                   <  2:360259/150‑1 (MQ=255)
                                                                                                    ttGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCa                                                  <  1:542718/150‑1 (MQ=255)
                                                                                                    ttGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCa                                                  <  2:421541/150‑1 (MQ=255)
                                                                                                        cAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCaataa                                              >  2:1052747/1‑150 (MQ=255)
                                                                                                                 aatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGa                                     >  2:1412230/1‑150 (MQ=255)
                                                                                                                      gAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAt                                >  2:996130/1‑150 (MQ=255)
                                                                                                                        gCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAttt                              <  2:827602/150‑1 (MQ=255)
                                                                                                                          aCTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTc                            <  1:1052747/150‑1 (MQ=255)
                                                                                                                             ttCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAAc                         <  1:1532073/150‑1 (MQ=255)
                                                                                                                              tCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACt                        <  1:1393420/150‑1 (MQ=255)
                                                                                                                              tCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACt                        >  1:377456/1‑150 (MQ=255)
                                                                                                                                ggcgtaTTCAC‑AAGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttt                      >  1:749553/6‑150 (MQ=255)
                                                                                                                                ggcgtaTTCAC‑AAGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttt                      >  2:1172389/6‑150 (MQ=255)
                                                                                                                                aaTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttt                      >  2:342037/1‑150 (MQ=255)
                                                                                                                                 gcgtaTTCAC‑AAGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTa                     >  1:317047/5‑150 (MQ=255)
                                                                                                                                  tAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTaa                    >  1:988604/1‑150 (MQ=255)
                                                                                                                                   aaaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAAc                   <  1:181861/150‑1 (MQ=255)
                                                                                                                                    aaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACa                  <  2:1608699/150‑1 (MQ=255)
                                                                                                                                        cACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCg              >  2:959058/1‑150 (MQ=255)
                                                                                                                                         aCGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGt             <  2:1497213/150‑1 (MQ=255)
                                                                                                                                          cGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTg            >  2:1448306/1‑150 (MQ=255)
                                                                                                                                          cGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTg            >  1:554755/1‑150 (MQ=255)
                                                                                                                                          cGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTg            >  2:278372/1‑150 (MQ=255)
                                                                                                                                          acaaGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGt             >  1:986489/3‑150 (MQ=255)
                                                                                                                                          acaaGCTGATGAACAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTg            >  2:453704/3‑150 (MQ=255)
                                                                                                                                           gAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTgg           <  2:1432602/150‑1 (MQ=255)
                                                                                                                                           gAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTgg           <  1:959058/150‑1 (MQ=255)
                                                                                                                                             aGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGtt         <  2:332261/150‑1 (MQ=255)
                                                                                                                                                tGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAAc      <  1:329366/150‑1 (MQ=255)
                                                                                                                                                    catcaGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCtt  >  1:204836/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTAACAATCGTGGTTAACGCTT  >  NC_000913/2491118‑2491415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑